Crosstalk between protein kinase A and the HOG pathway under impaired biosynthesis of complex sphingolipids in budding yeast

FEBS Journal ◽  
2021 ◽  
Author(s):  
Atsuya Urita ◽  
Yohei Ishibashi ◽  
Ryotaro Kawaguchi ◽  
Yukimi Yanase ◽  
Motohiro Tani
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Budding Yeast ◽  
Hog Pathway ◽  
Kinase A ◽  
Complex Sphingolipids

scholarly journals Protein kinase A controls yeast growth in visible light

BMC Biology ◽  
2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Mikael Molin ◽  
Katarina Logg ◽  
Kristofer Bodvard ◽  
Ken Peeters ◽  
Annabelle Forsmark ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Visible Light ◽  
Protein Kinase A ◽  
Rna Polymerase Ii ◽  
Gene Deletion ◽  
Uv Light ◽  
Light Exposure ◽  
Light Stress ◽  
Hog Pathway ◽  
Kinase A

Abstract Background A wide variety of photosynthetic and non-photosynthetic species sense and respond to light, having developed protective mechanisms to adapt to damaging effects on DNA and proteins. While the biology of UV light-induced damage has been well studied, cellular responses to stress from visible light (400–700 nm) remain poorly understood despite being a regular part of the life cycle of many organisms. Here, we developed a high-throughput method for measuring growth under visible light stress and used it to screen for light sensitivity in the yeast gene deletion collection. Results We found genes involved in HOG pathway signaling, RNA polymerase II transcription, translation, diphthamide modifications of the translational elongation factor eEF2, and the oxidative stress response to be required for light resistance. Reduced nuclear localization of the transcription factor Msn2 and lower glycogen accumulation indicated higher protein kinase A (cAMP-dependent protein kinase, PKA) activity in many light-sensitive gene deletion strains. We therefore used an ectopic fluorescent PKA reporter and mutants with constitutively altered PKA activity to show that repression of PKA is essential for resistance to visible light. Conclusion We conclude that yeast photobiology is multifaceted and that protein kinase A plays a key role in the ability of cells to grow upon visible light exposure. We propose that visible light impacts on the biology and evolution of many non-photosynthetic organisms and have practical implications for how organisms are studied in the laboratory, with or without illumination.

scholarly journals A prion-like domain of Tpk2 catalytic subunit of protein kinase A modulates P-body formation in response to stress in budding yeast

2021 ◽  
Vol 1868 (1) ◽  
pp. 118884
Author(s):  
Carla E. Barraza ◽  
Clara A. Solari ◽  
Jimena Rinaldi ◽  
Lucas Ojeda ◽  
Silvia Rossi ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Budding Yeast ◽  
Catalytic Subunit ◽  
Body Formation ◽  
Response To Stress ◽  
Kinase A

Expression and mutation analysis of regulatory subunit Iα of protein kinase A in undifferentiated and anaplastic thyroid carcinoma

2004 ◽  
Vol 112 (S 1) ◽  
Author(s):  
M Lippert ◽  
SY Sheu ◽  
K Worm ◽  
M Wichert ◽  
KW Schmid ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Thyroid Carcinoma ◽  
Protein Kinase A ◽  
Mutation Analysis ◽  
Anaplastic Thyroid Carcinoma ◽  
Regulatory Subunit ◽  
Kinase A

Regulation of spontaneous and induced resumption of meiosis in mouse oocytes by different intracellular pathways

Reproduction ◽  
2000 ◽  
pp. 377-383 ◽  
Author(s):  
L Leonardsen ◽  
A Wiersma ◽  
M Baltsen ◽  
AG Byskov ◽  
CY Andersen
Keyword(s):  
Signal Transduction ◽  
Protein Kinase ◽  
Protein Kinase A ◽  
Mitogen Activated Protein Kinase ◽  
Meiotic Maturation ◽  
Mouse Oocytes ◽  
Mitogen Activated Protein ◽  
Dependent Signal ◽  
Kinase Pathway ◽  
Kinase A

The mitogen-activated protein kinase-dependent and the cAMP-protein kinase A-dependent signal transduction pathways were studied in cultured mouse oocytes during induced and spontaneous meiotic maturation. The role of the mitogen-activated protein kinase pathway was assessed using PD98059, which specifically inhibits mitogen-activated protein kinase 1 and 2 (that is, MEK1 and MEK2), which activates mitogen-activated protein kinase. The cAMP-dependent protein kinase was studied by treating oocytes with the protein kinase A inhibitor rp-cAMP. Inhibition of the mitogen-activated protein kinase pathway by PD98059 (25 micromol l(-1)) selectively inhibited the stimulatory effect on meiotic maturation by FSH and meiosis-activating sterol (that is, 4,4-dimethyl-5alpha-cholest-8,14, 24-triene-3beta-ol) in the presence of 4 mmol hypoxanthine l(-1), whereas spontaneous maturation in the absence of hypoxanthine was unaffected. This finding indicates that different signal transduction mechanisms are involved in induced and spontaneous maturation. The protein kinase A inhibitor rp-cAMP induced meiotic maturation in the presence of 4 mmol hypoxanthine l(-1), an effect that was additive to the maturation-promoting effect of FSH and meiosis-activating sterol, indicating that induced maturation also uses the cAMP-protein kinase A-dependent signal transduction pathway. In conclusion, induced and spontaneous maturation of mouse oocytes appear to use different signal transduction pathways.

Regulation of Hepatic Glucose Production by Transforming Growth Factor Beta 1 via Protein Kinase A

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 2441-PUB ◽  
Author(s):  
QUAN PAN ◽  
YUNMEI CHEN ◽  
HUI YAN ◽  
WANBAO YANG ◽  
ZHENG SHEN ◽  
...  
Keyword(s):  
Growth Factor ◽  
Protein Kinase ◽  
Protein Kinase A ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Hepatic Glucose Production ◽  
Glucose Production ◽  
Hepatic Glucose ◽  
Growth Factor Beta ◽  
Kinase A
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