Flow cytometry analysis of DNA ploidy levels and protein profiles distinguish between populations ofLumbriculus(Annelida: Clitellata)

2016 ◽  
Vol 135 (4) ◽  
pp. 385-399 ◽  
Author(s):  
Kay A. Tweeten ◽  
Samantha J. Morris
Keyword(s):  
Flow Cytometry ◽  
Dna Ploidy ◽  
Protein Profiles ◽  
Flow Cytometry Analysis ◽  
Ploidy Levels

scholarly journals MICROPROPAGATION AND PLOIDY STABILITY OF Lippia lacunosa Mart. & Schauer: AN ENDANGERED BRAZILIAN MEDICINAL PLANT

2019 ◽  
Vol 6 (1) ◽  
pp. 1-7
Author(s):  
Diego Pandeló José ◽  
José Marcello Salabert De Campos ◽  
Lyderson Facio Viccini ◽  
Emilly Ruas Alkimim ◽  
Marcelo De Oliveira Santos
Keyword(s):  
Flow Cytometry ◽  
Medicinal Plant ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Naphthalene Acetic Acid ◽  
Flow Cytometry Analysis ◽  
Ploidy Levels

Lippia lacunosa is a Brazilian savanna plant that belongs to the Verbenaceae family. It has been used in folk medicine as a treatment for different diseases. This species represents an endangered Brazilian medicinal plant, and this is the first report documenting a reliable protocol for the in vitro propagation and regeneration of L. lacunosa. Axenic explants were cultivated in MS medium containing different concentrations of naphthalene acetic acid (NAA) to induce root growth. The mean shoot length and the number of roots were highest with 0.06 mg·L-1 NAA. The highest number of buds in shoot regeneration was induced with 2 mg·L-1 6-benzylaminopurine (BA). To obtain a long-term culture, the dwarf shoots were elongated on MS media containing 0.5 mg·L-1 BA alternated with MS containing 2 mg·L-1 BA every 40 days. In the present protocol, the long-term shoots retained the ability to root even after long periods of BA treatment. In addition, we evaluated the nuclear DNA content and ploidy levels, including the occurrence of endopolyploidy, in long-term micropropagated plant leaves using flow cytometry analysis. The plants propagated in vitro over several years possessed nuclear DNA contents ranging from 2.940 to 3.095 pg, and no differences in DNA content were found among in vitro plants or between these plants and the control (L. lacunosa from a greenhouse with a DNA content of 3.08 pg). The flow cytometry analysis also demonstrated that there was no polyploidization. The present study will be useful for biotechnological approaches and provides the first estimate of the nuclear DNA content of this species using flow cytometry.

scholarly journals Differences in Winter Browning among Japanese-cedar Cultivars Are Not Due to Variation in Ploidy Levels

HortScience ◽  
2011 ◽  
Vol 46 (11) ◽  
pp. 1465-1467
Author(s):  
Ryan N. Contreras ◽  
Ron Determann ◽  
Mara Friddle
Keyword(s):  
Flow Cytometry ◽  
Enzyme Activity ◽  
Quantitative Analysis ◽  
Growth Form ◽  
Japanese Cedar ◽  
Antioxidant Enzyme Activity ◽  
Flow Cytometry Analysis ◽  
Chromosome Counts ◽  
Ploidy Levels ◽  
Number Of Authors

There is a great deal of variation among japanese-cedar cultivars with regard to growth form, foliar characteristics, and winter browning. Differences in winter browning have been observed and documented by a number of authors. Previous research has established that there are differences in winter foliage color between cultivars included in the current study; however, no quantitative analysis under standardized conditions was conducted. Because of a previous report that tetraploid forms of japanese-cedar remain green during winter as a result of increased antioxidant enzyme activity, we hypothesized that cultivars that exhibit reduced winter browning were polyploids. We screened 56 accessions of japanese-cedar using flow cytometry analysis of 4′,6-diamidino-2-phenylindole (DAPI)-stained nuclei and performed chromosome counts on three cultivars. All accessions were diploid (2n = 2x = 22), although there were significant differences in genome sizes among the cultivars. Holoploid genome sizes ranged from 18.9 pg for var. sinensis JCRA to 22.3 pg for ‘Viridis’ with a mean of 20.1 pg. Chromosome counts for cultivars Ogon, Oye Keme, and Viridis supported the flow cytometry results. Although the underlying cause of the variability in morphology and winter browning among cultivars is unclear, our results show that differences in ploidy level are not responsible, because all tested genotypes were diploid. Chemical name: 4′,6-diamidino-2-phenylindole (DAPI).

DNA ploidy level variability of some fescues (Festuca subg. Festuca, Poaceae) from Central and Southern Europe measured in fresh plants and herbarium specimens

Biologia ◽  
2008 ◽  
Vol 63 (3) ◽  
Author(s):  
Petr Šmarda
Keyword(s):  
Flow Cytometry ◽  
Czech Republic ◽  
Ploidy Level ◽  
New Records ◽  
Dna Ploidy ◽  
Herbarium Specimens ◽  
Southern Europe ◽  
Ploidy Levels ◽  
The Czech Republic ◽  
First Time

AbstractUsing flow cytometry in fresh plants and herbarium vouchers, DNA ploidy levels for 411 individuals of 44 taxa of the genus Festuca, including 4 natural hybrids, originating from 237 sites in Austria, Bulgaria, Croatia, Czech Republic, Estonia, Germany, Hungary, Italy, Poland, Romania, Slovakia, Slovenia, and Switzerland were estimated. The following taxa and DNA ploidy levels are reported: F. airoides (2n ≈ 2x), F. alpestris (2n ≈ 2x), F. alpina s.l. (2n ≈ 2x), F. amethystina subsp. amethystina (2n ≈ 4x), F. bosniaca subsp. bosniaca (2n ≈ 2x), F. brevipila (2n ≈ 6x), F. bucegiensis (2n ≈ 2x), F. carnuntina (2n ≈ 6x), F. csikhegyensis (2n ≈ 4x), F. csikhegyensis × F. eggleri (2n ≈ 4x), F. dalmatica (2n ≈ 4x), F. duvalii (2n ≈ 4x), F. eggleri (2n ≈ 2x, 4x), F. filiformis (2n ≈ 2x), F. glauca (2n ≈ 6x), F. heterophylla (2n ≈ 4x), F. inops (2n ≈ 2x), F. laevigata (2n ≈ 8x), F. laxa (2n ≈ 4x), F. lemanii (2n ≈ 6x), F. norica (2n ≈ 2x), F. ovina subsp. ovina (2n ≈ 2x), F. ovina subsp. guesfalica (2n ≈ 4x), F. ovina × F. pallens (2n ≈ 4x), F. pallens (2n ≈ 2x, 3x), F. pallens × F. pseudodalmatica (2n ≈ 3x, 4x), F. pirinica (2n ≈ 2x), F. polesica (2n ≈ 2x), F. psammophila subsp. dominii (2n ≈ 2x), F. pseudodalmatica (2n ≈ 4x), F. pseudovina (2n ≈ 2x), F. quadriflora (2n ≈ 4x), F. rupicola (2n ≈ 6x), F. rupicola × F. vaginata (2n ≈ 3x, 4x), F. saxatilis (2n ≈ 6x), F. stricta subsp. bauzanina (2n ≈ 8x), F. supina (2n ≈ 4x), F. tatrae (2n ≈ 2x), F. valesiaca (2n ≈ 2x), F. versicolor subsp. pallidula (2n ≈ 2x), F. versicolor subsp. versicolor (2n ≈ 2x), F. violacea subsp. puccinellii (2n ≈ 2x), F. wagneri (2n ≈ 4x), F. xanthina (2n ≈ 2x). In F. pallens, up to 12-year-old herbarium specimens were proved to be suitable for DNA ploidy level measurements with flow cytometry.DNA ploidy levels of F. bucegiensis, F. bosniaca, and F. versicolor subsp. pallidula are reported here for the first time. The taxonomy of some polyploid complexes and several records of mixed ploidy level populations are briefly discussed. Festuca pseudodalmatica and its hybrid F. × krizoviensis were first recognised as native to the Czech Republic, and F. brevipila as native to Hungary. Also some new records of F. filiformis, F. brevipila, and F. wagneri from Slovakia are reported.

Evaluation of DNA ploidy in dysplastic and Spitz nevi by flow cytometry

1990 ◽  
Vol 17 (6) ◽  
pp. 342-347 ◽  
Author(s):  
Thomas S. Winokur ◽  
Juan P. Palazzo ◽  
Wayne C. Johnson ◽  
Paul H. Duray
Keyword(s):  
Flow Cytometry ◽  
Dna Ploidy ◽  
Spitz Nevi

scholarly journals A Synergic Potential of Antimicrobial Peptides against Pseudomonas syringae pv. actinidiae

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1461
Author(s):  
Nuno Mariz-Ponte ◽  
Laura Regalado ◽  
Emil Gimranov ◽  
Natália Tassi ◽  
Luísa Moura ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Antimicrobial Peptides ◽  
Pseudomonas Syringae ◽  
Pathogenic Bacteria ◽  
High Efficiency ◽  
Bacterial Canker ◽  
Membrane Permeation ◽  
Flow Cytometry Analysis ◽  
Pathogenic Agent ◽  
Peptide Mixtures

Pseudomonas syringae pv. actinidiae (Psa) is the pathogenic agent responsible for the bacterial canker of kiwifruit (BCK) leading to major losses in kiwifruit productions. No effective treatments and measures have yet been found to control this disease. Despite antimicrobial peptides (AMPs) having been successfully used for the control of several pathogenic bacteria, few studies have focused on the use of AMPs against Psa. In this study, the potential of six AMPs (BP100, RW-BP100, CA-M, 3.1, D4E1, and Dhvar-5) to control Psa was investigated. The minimal inhibitory and bactericidal concentrations (MIC and MBC) were determined and membrane damaging capacity was evaluated by flow cytometry analysis. Among the tested AMPs, the higher inhibitory and bactericidal capacity was observed for BP100 and CA-M with MIC of 3.4 and 3.4–6.2 µM, respectively and MBC 3.4–10 µM for both. Flow cytometry assays suggested a faster membrane permeation for peptide 3.1, in comparison with the other AMPs studied. Peptide mixtures were also tested, disclosing the high efficiency of BP100:3.1 at low concentration to reduce Psa viability. These results highlight the potential interest of AMP mixtures against Psa, and 3.1 as an antimicrobial molecule that can improve other treatments in synergic action.

scholarly journals Flow cytometry analysis of anti-polyethylene glycol antibodies in human plasma

2021 ◽  
Vol 8 ◽  
pp. 148-154
Author(s):  
Jia-Long Fang ◽  
Frederick A. Beland ◽  
Yangshun Tang ◽  
Steve R. Roffler
Keyword(s):  
Flow Cytometry ◽  
Polyethylene Glycol ◽  
Human Plasma ◽  
Flow Cytometry Analysis

scholarly journals Flow Cytometry-Based Determination of Ploidy from Dried Leaf Specimens in Genomically Complex Collections of the Tropical Forage Grass Urochloa s. l.

Genes ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 957
Author(s):  
Paulina Tomaszewska ◽  
Till K. Pellny ◽  
Luis M. Hernández ◽  
Rowan A. C. Mitchell ◽  
Valheria Castiblanco ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Germplasm Collection ◽  
Forage Grass ◽  
Breeding Programs ◽  
Robust Identification ◽  
Ploidy Levels ◽  
Sustainable Improvement ◽  
Tropical Forage ◽  
Relative Differences

Urochloa (including Brachiaria, Megathyrus and some Panicum) tropical grasses are native to Africa and are now, after selection and breeding, planted worldwide, particularly in South America, as important forages with huge potential for further sustainable improvement and conservation of grasslands. We aimed to develop an optimized approach to determine ploidy of germplasm collection of this tropical forage grass group using dried leaf material, including approaches to collect, dry and preserve plant samples for flow cytometry analysis. Our methods enable robust identification of ploidy levels (coefficient of variation of G0/G1 peaks, CV, typically <5%). Ploidy of some 348 forage grass accessions (ploidy range from 2x to 9x), from international genetic resource collections, showing variation in basic chromosome numbers and reproduction modes (apomixis and sexual), were determined using our defined standard protocol. Two major Urochloa agamic complexes are used in the current breeding programs at CIAT and EMBRAPA: the ’brizantha’ and ’humidicola’ agamic complexes are variable, with multiple ploidy levels. Some U. brizantha accessions have odd level of ploidy (5x), and the relative differences in fluorescence values of the peak positions between adjacent cytotypes is reduced, thus more precise examination of this species is required. Ploidy measurement of U. humidicola revealed aneuploidy.

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