Reactive Oxygen Species Selectively Deplete Normal T Lymphocytes Via a Hydroxyl Radical Dependent Mechanism

Recently, the toxic hydroxyl radical (·OH) has received wide interest in inducing cell apoptosis by increasing the intracellular reactive oxygen species (ROS) levels. Herein, a cationic polymer (MV-PAH) was rationally...

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JP-8 Induces Immune Suppression via a Reactive Oxygen Species NF-κβ–Dependent Mechanism

Toxicological Sciences ◽

10.1093/toxsci/kfn262 ◽

2008 ◽

Vol 108 (1) ◽

pp. 100-109 ◽

Cited By ~ 7

Author(s):

Gerardo Ramos ◽

Alberto Y. Limon-Flores ◽

Stephen E. Ullrich

Keyword(s):

Reactive Oxygen Species ◽

Immune Suppression ◽

Reactive Oxygen ◽

Oxygen Species ◽

Dependent Mechanism

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Doxorubicin activates nuclear factor of activated T-lymphocytes and Fas ligand transcription: role of mitochondrial reactive oxygen species and calcium

Biochemical Journal ◽

10.1042/bj20050285 ◽

2005 ◽

Vol 389 (2) ◽

pp. 527-539 ◽

Cited By ~ 119

Author(s):

Shasi V. Kalivendi ◽

Eugene A. Konorev ◽

Sonya Cunningham ◽

Sravan K. Vanamala ◽

Eugene H. Kaji ◽

...

Keyword(s):

Reactive Oxygen Species ◽

T Lymphocytes ◽

Fas Ligand ◽

Reactive Oxygen ◽

Nuclear Factor ◽

Oxygen Species ◽

Rat Cardiomyocytes ◽

Activated T Lymphocytes ◽

Pre Treatment ◽

Fas L

Doxorubicin (DOX), a widely used antitumour drug, causes dose-dependent cardiotoxicity. Cardiac mitochondria represent a critical target organelle of toxicity during DOX chemotherapy. Proposed mechanisms include generation of ROS (reactive oxygen species) and disturbances in mitochondrial calcium homoeostasis. In the present study, we probed the mechanistic link between mitochondrial ROS and calcium in the embryonic rat heart-derived H9c2 cell line and in adult rat cardiomyocytes. The results show that DOX stimulates calcium/calcineurin-dependent activation of the transcription factor NFAT (nuclear factor of activated T-lymphocytes). Pre-treatment of cells with an intracellular calcium chelator abrogated DOX-induced nuclear NFAT translocation, Fas L (Fas ligand) expression and caspase activation, as did pre-treatment of cells with a mitochondria-targeted antioxidant, Mito-Q (a mitochondria-targeted antioxidant consisting of a mixture of mitoquinol and mitoquinone), or with adenoviral-over-expressed antioxidant enzymes. Treatment with GPx-1 (glutathione peroxidase 1), MnSOD (manganese superoxide dismutase) or a peptide inhibitor of NFAT also inhibited DOX-induced nuclear NFAT translocation. Pre-treatment of cells with a Fas L neutralizing antibody abrogated DOX-induced caspase-8- and -3-like activities during the initial stages of apoptosis. We conclude that mitochondria-derived ROS and calcium play a key role in stimulating DOX-induced ‘intrinsic and extrinsic forms’ of apoptosis in cardiac cells with Fas L expression via the NFAT signalling mechanism. Implications of ROS- and calcium-dependent NFAT signalling in DOX-induced apoptosis are discussed.

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Manganese-Oxidizing Antarctic Bacteria (Mn-Oxb) Release Reactive Oxygen Species (ROS) as Secondary Mn(II) Oxidation Mechanisms to Avoid Toxicity

Biology ◽

10.3390/biology10101004 ◽

2021 ◽

Vol 10 (10) ◽

pp. 1004

Author(s):

Ignacio Jofré ◽

Francisco Matus ◽

Daniela Mendoza ◽

Francisco Nájera ◽

Carolina Merino

Keyword(s):

Reactive Oxygen Species ◽

Reactive Oxygen ◽

Soil Formation ◽

Ros Production ◽

Oxygen Species ◽

Ros Scavenging ◽

Cell Protection ◽

Temperature Dependent ◽

Antarctic Soils ◽

Dependent Mechanism

Manganese (Mn) oxidation is performed through oxidative Mn-oxidizing bacteria (MnOxb) as the main bio-weathering mechanism for Mn(III/IV) deposits during soil formation. However, with an increase in temperature, the respiration rate also increases, producing Reactive Oxygen Species (ROS) as by-products, which are harmful to microbial cells. We hypothesize that bacterial ROS oxidize Mn(II) to Mn(III/IV) as a secondary non-enzymatic temperature-dependent mechanism for cell protection. Fourteen MnOxb were isolated from Antarctic soils under the global warming effect, and peroxidase (PO) activity, ROS, and Mn(III/IV) production were evaluated for 120 h of incubation at 4 °C, 15 °C, and 30 °C. ROS contributions to Mn oxidation were evaluated in Arthrobacter oxydans under antioxidant (Trolox) and ROS-stimulated (menadione) conditions. The Mn(III/IV) concentration increased with temperature and positively correlated with ROS production. ROS scavenging with Trolox depleted the Mn oxidation, and ROS-stimulant increased the Mn precipitation in A. oxydans. Increasing the Mn(II) concentration caused a reduction in the membrane potential and bacterial viability, which resulted in Mn precipitation on the bacteria surface. In conclusion, bacterial ROS production serves as a complementary non-enzymatic temperature-dependent mechanism for Mn(II) oxidation as a response in warming environments.

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Decrease in generation of reactive oxygen species by neutrophils from patients with infectious mononucleosis: role of suppressor T lymphocytes

Blood ◽

10.1182/blood.v64.5.994.bloodjournal645994 ◽

1984 ◽

Vol 64 (5) ◽

pp. 994-999

Author(s):

Y Niwa ◽

T Sakane ◽

Y Miyachi ◽

T Kanoh ◽

K Somiya

Keyword(s):

Reactive Oxygen Species ◽

T Lymphocytes ◽

Infectious Mononucleosis ◽

Disease Onset ◽

Reactive Oxygen ◽

Control Group ◽

Ros Generation ◽

Oxygen Species ◽

Suppressor T Lymphocytes ◽

Subsets Of T Lymphocytes

We assessed the generation of reactive oxygen species (ROS: O2-, H2O2, OH . , chemiluminescence) by neutrophils and monocytes from six patients with infectious mononucleosis, ten patients with other viral diseases, and ten normal controls. Neutrophils from infectious mononucleosis patients showed markedly decreased generation of all reactive oxygen species, compared with the two control groups; this abnormality persisted for four to eight weeks after disease onset. Monocytes from these patients generated normal levels of ROS. Normal neutrophils incubated with T lymphocytes from infectious mononucleosis patients generated significantly less of each ROS than did those incubated with T cells from either control group. T cell-mediated suppression of ROS generation required both OKT4+ cells from infectious mononucleosis patients and OKT8+ cells from either patients or normals. We conclude that the generation of reaction oxygen species in neutrophils is suppressed in patients with infectious mononucleosis, at least in part, by interacting subsets of T lymphocytes.

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Role of mitochondrial superoxide dismutase in contraction-induced generation of reactive oxygen species in skeletal muscle extracellular space

AJP Cell Physiology ◽

10.1152/ajpcell.00322.2003 ◽

2004 ◽

Vol 286 (5) ◽

pp. C1152-C1158 ◽

Cited By ~ 51

Author(s):

A. McArdle ◽

J. van der Meulen ◽

G. L. Close ◽

D. Pattwell ◽

H. Van Remmen ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Hydroxyl Radical ◽

Hydroxyl Radicals ◽

Extracellular Space ◽

Superoxide Anion ◽

Reactive Oxygen ◽

Oxygen Species ◽

Superoxide Anions ◽

Wild Type ◽

Mnsod Activity

Contractions of skeletal muscles produce increases in concentrations of superoxide anions and activity of hydroxyl radicals in the extracellular space. The sources of these reactive oxygen species are not clear. We tested the hypothesis that, after a demanding isometric contraction protocol, the major source of superoxide and hydroxyl radical activity in the extracellular space of muscles is mitochondrial generation of superoxide anions and that, with a reduction in MnSOD activity, concentration of superoxide anions in the extracellular space is unchanged but concentration of hydroxyl radicals is decreased. For gastrocnemius muscles from adult (6–8 mo old) wild-type ( Sod2+/+) mice and knockout mice heterozygous for the MnSOD gene ( Sod2+/-), concentrations of superoxide anions and hydroxyl radical activity were measured in the extracellular space by microdialysis. A 15-min protocol of 180 isometric contractions induced a rapid, equivalent increase in reduction of cytochrome c as an index of superoxide anion concentrations in the extracellular space of Sod2+/+ and Sod2+/- mice, whereas hydroxyl radical activity measured by formation of 2,3-dihydroxybenzoate from salicylate increased only in the extracellular space of muscles of Sod2+/+ mice. The lack of a difference in increase in superoxide anion concentration in the extracellular space of Sod2+/+ and Sod2+/- mice after the contraction protocol supported the hypothesis that superoxide anions were not directly derived from mitochondria. In contrast, the data obtained suggest that the increase in hydroxyl radical concentration in the extracellular space of muscles from wild-type mice after the contraction protocol most likely results from degradation of hydrogen peroxide generated by MnSOD activity.

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Insight on the generation of reactive oxygen species in the CaO2/Fe(II) Fenton system and the hydroxyl radical advancing strategy

Chemical Engineering Journal ◽

10.1016/j.cej.2018.07.124 ◽

2018 ◽

Vol 353 ◽

pp. 657-665 ◽

Cited By ~ 15

Author(s):

Yunfei Xue ◽

Qian Sui ◽

Mark L. Brusseau ◽

Xiang Zhang ◽

Zhaofu Qiu ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Hydroxyl Radical ◽

Reactive Oxygen ◽

Oxygen Species ◽

Fenton System

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Author Correction: FTY720 Induces Autophagy-Associated Apoptosis in Human Oral Squamous Carcinoma Cells, in Part, through a Reactive Oxygen Species/Mcl-1-Dependent Mechanism

Scientific Reports ◽

10.1038/s41598-020-60297-8 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Li-Yuan Bai ◽

Chang-Fang Chiu ◽

Shih-Jiuan Chiu ◽

Po-Chen Chu ◽

Jing-Ru Weng

Keyword(s):

Reactive Oxygen Species ◽

Reactive Oxygen ◽

Squamous Carcinoma ◽

Carcinoma Cells ◽

Oxygen Species ◽

Squamous Carcinoma Cells ◽

Oral Squamous Carcinoma ◽

Dependent Mechanism

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Reactivity of Several Reactive Oxygen Species (ROS) with the Sesquiterpene Cacalol

Natural Product Communications ◽

10.1177/1934578x0800300403 ◽

2008 ◽

Vol 3 (4) ◽

pp. 1934578X0800300

Author(s):

Manuel Jiménez-Estrada ◽

Ricardo Reyes-Chilpa ◽

Arturo Navarro-Ocaña ◽

Daniel Arrieta-Báez

Keyword(s):

Reactive Oxygen Species ◽

Hydroxyl Radical ◽

Singlet Oxygen ◽

Furan Ring ◽

Reactive Oxygen ◽

Antioxidant Effect ◽

Oxygen Species ◽

Site Reaction ◽

Hexanedioic Acid ◽

Antioxidant Effects

To analyze the antioxidant effects of cacalol we determined its reactivity with different reactive oxygen species (ROS). Cacalol gave rise to cacalone by a specific site reaction with a hydroxyl radical. Singlet oxygen reacted only with the double bond of the furan ring, causing its rupture. On the other hand, ozone reacted with all double bonds in cacalol affording 2-methyl-hexanedioic acid as an end product. No reaction was observed with either superoxide or hydrogen peroxide. The potential antioxidant effect of cacalol as a scavenger of hydroxyl radical and singlet oxygen could be related to its function in the plant roots.

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Reactivity of graphene oxide with reactive oxygen species (hydroxyl radical, singlet oxygen, and superoxide anion)

Environmental Science Nano ◽

10.1039/c9en00693a ◽

2019 ◽

Vol 6 (12) ◽

pp. 3734-3744 ◽

Cited By ~ 2

Author(s):

Hsin-Se Hsieh ◽

Richard G. Zepp

Keyword(s):

Reactive Oxygen Species ◽

Graphene Oxide ◽

Hydroxyl Radical ◽

Singlet Oxygen ◽

Superoxide Anion ◽

Reactive Oxygen ◽

Toxic Effects ◽

Oxygen Species ◽

Ecological Risks

Increases in the production and applications of graphene oxide (GO), coupled with reports of its toxic effects, are raising concerns about its health and ecological risks.

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