MORPHOLOGICAL AND QUANTITATIVE CHANGES IN THE POPULATION OF CELL ORGANELLES DURING MICROSPOROGENESIS OF PINUS SYLVESTRIS L. III. MORPHOLOGICAL CHANGES DURING THE TETRAD STAGE AND IN THE YOUNG MICROSPORE. A QUANTITATIVE APPROACH TO THE CHANGES IN THE POPUL

1971 ◽  
Vol 20 (5) ◽  
pp. 498-523 ◽  
Author(s):  
M. Th. M. Willemse
Keyword(s):  
Pinus Sylvestris ◽  
Morphological Changes ◽  
Quantitative Approach ◽  
Cell Organelles ◽  
Tetrad Stage ◽  
Quantitative Changes

scholarly journals Quantifying the Rate, Degree, and Heterogeneity of Morphological Change during an Epithelial to Mesenchymal Transition Using Digital Holographic Cytometry

2020 ◽  
Vol 10 (14) ◽  
pp. 4726
Author(s):  
Sofia Kamlund ◽  
Birgit Janicke ◽  
Kersti Alm ◽  
Robert L. Judson-Torres ◽  
Stina Oredsson
Keyword(s):  
Cell Line ◽  
Morphological Change ◽  
Efficient Method ◽  
Mammalian Cells ◽  
Epithelial To Mesenchymal Transition ◽  
Morphological Changes ◽  
Cell Populations ◽  
Label Free ◽  
Mesenchymal Transition ◽  
Quantitative Changes

Cells in complex organisms can transition between epithelial and mesenchymal phenotypes during both normal and malignant physiological events. These two phenotypes are not binary, but rather describe a spectrum of cell states along an axis. Mammalian cells can undergo dynamic and heterogenous bidirectional interconversions along the epithelial–mesenchymal phenotypic (EMP) spectrum, and such transitions are marked by morphological change. Here, we exploit digital holographic cytometry (DHC) to develop a tractable method for monitoring the degree, kinetics, and heterogeneity of epithelial and mesenchymal phenotypes in adherent mammalian cell populations. First, we demonstrate that the epithelial and mesenchymal states of the same cell line present distinct DHC-derived morphological features. Second, we identify quantitative changes in these features that occur hours after induction of the epithelial to mesenchymal transition (EMT). We apply this approach to achieve label-free tracking of the degree and the rate of EMP transitions. We conclude that DHC is an efficient method to investigate morphological changes during transitions between epithelial and mesenchymal states.

scholarly journals Alterations of morphology of lymphoid organs and peripheral blood indicators under the influence of gold nanoparticles in rats

2016 ◽  
Vol 09 (01) ◽  
pp. 1640004 ◽  
Author(s):  
Alla B. Bucharskaya ◽  
Svetlana S. Pakhomy ◽  
Olga V. Zlobina ◽  
Galina N. Maslyakova ◽  
Olga V. Matveeva ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Peripheral Blood ◽  
Morphological Changes ◽  
Cancer Diagnostics ◽  
Lymphoid Organs ◽  
Male Rats ◽  
Laboratory Animals ◽  
Peroral Administration ◽  
Proliferation And Differentiation ◽  
Quantitative Changes

At present, gold nanoparticles (GNPs) are widely used in biomedical applications such as cancer diagnostics and therapy. Accordingly, the potential toxicity hazards of these nanomaterials and human safety concerns are gaining significant attention. Here, we report the effects of prolonged peroral administration of GNPs with different sizes (2, 15 and 50[Formula: see text]nm) on morphological changes in lymphoid organs and indicators of peripheral blood of laboratory animals. The experiment was conducted on 24 white mongrel male rats weighing 180–220[Formula: see text]g, gold nanospheres sizes 2, 15 and 50[Formula: see text]nm were administered orally for 15 days at a dosage of 190[Formula: see text][Formula: see text]g/kg of animal body weight. The GNPs were conjugated with polyethylene glycol to increase their biocompatibility and bioavailability. The size-dependent decrease of the number of neutrophils and lymphocytes was noted in the study of peripheral blood, especially pronounced after administration of GNPs with size of 50[Formula: see text]nm. The stimulation of myelocytic germ of hematopoiesis was recorded at morphological study of the bone marrow. The signs of strengthening of the processes of differentiation and maturation of cellular elements were found in lymph nodes, which were showed as the increasing number of immunoblasts and large lymphocytes. The quantitative changes of cellular component morphology of lymphoid organs due to activation of migration, proliferation and differentiation of immune cells indicate the presence of immunostimulation effect of GNPs.

scholarly journals MEDIA PEMBELAJARAN MOBILE LEARNING UNTUK MENINGKATKAN MOTIVASI DAN KEMAMPUAN PRAKTIKUM MAHASISWA STIKES KARSA HUSADA GARUT

2016 ◽  
Vol 14 (2) ◽  
Author(s):  
N. Ai Erlinawati ◽  
Uman Suherman ◽  
Deni Darmawan
Keyword(s):  
Experimental Method ◽  
Mobile Learning ◽  
Learning Motivation ◽  
Quantitative Approach ◽  
Motivation To Learn ◽  
Cell Organelles ◽  
Lecture Material ◽  
And Function ◽  
Biology Lab

Appropriate learning media do not support this lack of motivation to learn and the ability of students in the biology lab suspected in addition to the abstract nature of the study, as well as lectures. This research will study whether use of m-Learning  Mobile learning can increase learning motivation and the ability of student practicum STIKes Karsa Husada on the function and cells animal and plant?. The study was conducted and using a   quantitative approach with a   purely experimental method. This  Biology (Cell and Function in  Animals and  Plants)  using  Mobile-Learning  Media can improve student practicum, good skills preparation tool and material, work MFI, make preparations, observation preparations, identifying the cell, as well as skills distinguishing cell organelles. The results of these studies recommended to the faculty; it would be better if the Mobile learning is also used to deliver other materials. Because by using this method, the students are not only enthusiastic (motivated) to interact in lectures but also able to increase their works. Some other mediums of learning, besides Mobile-Learning, it suggested for the next researchers that they can examine the various advantages and accuracy of each medium of learning from the aspects of the lecture material. Keywords: Mobile-Learning, Motivation, Practicum

Needle ultrastructure and starch content in Scots pine and Norway spruce after ozone fumigation

1996 ◽  
Vol 74 (1) ◽  
pp. 67-76 ◽  
Author(s):  
Toini Holopainen ◽  
Seija Anttonen ◽  
Virpi Palomäki ◽  
Pirjo Kainulainen ◽  
Jarmo K. Holopainen
Keyword(s):  
Picea Abies ◽  
Pinus Sylvestris ◽  
Norway Spruce ◽  
Scots Pine ◽  
Starch Content ◽  
Ultrastructural Changes ◽  
Cell Organelles ◽  
Mesophyll Cells ◽  
Nursery Stock ◽  
Chloroplast Stroma

The ultrastructure of current and previous year needles of Scots pine (Pinus sylvestris L.) and Norway spruce (Picea abies L.) Karst. was studied after exposures to several different ozone concentrations (100 to 600 nL∙L−1) and doses in a chamber system. The ultrastructural changes observed in different experiments in both of these species were qualitatively very similar. Changes were observed in the mesophyll tissue, in which increased density of chloroplast stroma and an increase of ribosome-like granules were the early symptoms, followed by a decrease of the size of chloroplasts and starch grains. Simultaneously a decline of needle starch content could be measured. After fumigation with the lower concentrations, hypertrophy of microbodies were also apparent. When the seedlings were fumigated with the highest concentration (600 nL∙L−1) a subsequent disintegration of cytoplasm and other cell organelles and finally a collapse of the contents of the mesophyll cells, leading to visible injuries, was found. Scots pine nursery stock seedlings appeared to be about equal in sensitivity to ozone as Norway spruce clonal stock. The spruce clones showed differences in sensitivity to the ozone fumigations. Since the observed symptoms are consistent in two species through a concentration gradient of ozone, and also agree with the information dealing with ozone injuries in literature, it is possible to use ultrastructural observations for diagnosing the presence of ozone injuries. Keywords: ozone, Picea abies, Pinus sylvestris, needle ultrastructure, starch.

scholarly journals THE MORHOLOGIC EVALUATION OF MICROGLIAL REACTIONS IN WHITE MATTER OF THE BRAIN IN PATIENTS WITH CHRONIC HCV INFECTION

2016 ◽  
pp. 22-27
Author(s):  
A. M. Maybogin ◽  
M. K. Nedzvedz ◽  
A. I. Kurochkina
Keyword(s):  
White Matter ◽  
Morphological Changes ◽  
Hcv Infection ◽  
The Central Nervous System ◽  
Chronic Hcv Infection ◽  
Quantitative Changes ◽  
Chronic Hcv ◽  
The Brain ◽  
Excitotoxic Lesion ◽  
Different Parts

Disorders of the central nervous system observed in patients with chronic HCV infection seem to be associated with excitotoxic lesion of neurons caused by infected microglial cells. Despite the high interest in this problem, the morphological changes of infected microglia remain uninvestigated. The paper presents the results of the morphologic study of microglial reactions in white matter of the brain in 40 patients who died of chronic HCV-infection. The study found the structural and quantitative changes of infected microglia in different parts of the brain, which is caused by the activation of these cells by HCV.

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