Investigations on the Haemoglobin and Cell Volume in Certain Pathological Conditions in the Small Child.

Our previous studies have demonstrated that abnormally high amounts of calcium (Ca) and zinc (Zn) can be accumulated in human retina-choroid under pathological conditions and that barium (Ba), which was not detected in the eyes of healthy individuals, is deposited in the retina pigment epithelium (RPE), and to a lesser extent in the sensory retina and iris. In an attempt to understand how these cations can be accumulated in the vertebrate eye, a morphological and microanalytical study of the uptake and loss of specific cations (K, Ca,Ba,Zn) was undertaken with incubated Rana catesbiana isolated retina and RPE preparations. Large frogs (650-800 gms) were dark adapted, guillotined and their eyes enucleated in deep ruby light. The eyes were hemisected behind the ora serrata and the anterior portion of the eye removed. The eyecup was bisected along the plane of the optic disc and the two segments of retina peeled away from the RPE and incubated.

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An Ultrastructural Study of the Proliferation Patterns of the Reserve Cells of the Human Cervix Uteri

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100093651 ◽

1972 ◽

Vol 30 ◽

pp. 80-81

Author(s):

Takanori Sohda ◽

Hiroshi Saito ◽

Goro Asano ◽

Katsunari Fukushi ◽

Katsuya Suzuki ◽

...

Keyword(s):

Ultrastructural Study ◽

Age Groups ◽

Point Of View ◽

University Hospital ◽

Cervix Uteri ◽

Morphological Aspect ◽

Functional Aspect ◽

Pathological Conditions ◽

Human Cervix ◽

Morphological Patterns

Recently, the functional aspect as well as morphological aspect of the reserve cells in the cervix uteri drew much attention in view of the carcinogenesis in squamocolumunar junction. In this communication, the authors elucidate the ultrastructural features of the reserve cells in patients of various age groups visiting our university hospital and affiliated hospital.From conventional light microscopic point of view, the reserve cells tend to be pronounced in various pathological conditions, such as the persisting inflammation, proliferative disorders and irritation of hormones. The morphological patterns of the reserve cells from various stage and degree of irritation were observed.

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Surface Ultrastructure of Human Ectocervix in Certain Pathological Conditions

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100119636 ◽

1985 ◽

Vol 43 ◽

pp. 570-571

Author(s):

S. Mukherjee ◽

T. Guha ◽

B. Chakrabarti ◽

P. Chakrabarti

Keyword(s):

Epithelial Cells ◽

Marked Reduction ◽

Squamous Epithelium ◽

Malignant Tumour ◽

Surface Ultrastructure ◽

Normal Tissues ◽

Pathological Conditions ◽

Hexagonal Shape ◽

Columnar Cells ◽

Scanning Electron

The cervix is an important organ in reproduction. Its malfunction is frequently a factor for infertility. Ectocervix region does not appear to have received much attention although many studies have been reported on the endocervix. We report here our SEM observations on ectocervix in certain pathological conditions compared to normal ectocervix.Ectocervix specimens from human females with specific pathological disorders were processed for Scanning Electron Microscopy by conventional method and they were examined in a Philips SEM.The normal ectocervix is lined by flat layer of squamous epithelial cells with microridges (Fig. 1). These cells are known to be formed from columnar cells through metaplastic transformation. The cells of carcinoma-bearing ectocervix show a disorganised appearance (Fig. 2). In non-malignant tumour surface some cuboidal and few columnar cells were seen (Fig. 3). A cyst appears like an overgrowth on the surface of the squamous epithelium (Fig. 4). In ulcerated ectocervix a marked reduction of epithelial cells are observed (Fig. 5); the cells are devoid of microridges and, the large polygonal cells, as observed in normal tissues, have somehow acquired comparatively small hexagonal shape

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Minimally Invasive Approach for Wilms Tumor Treatment in a Small Child - A Case Report

Urology ◽

10.1016/j.urology.2020.12.003 ◽

2020 ◽

Author(s):

Alexandre Azevedo Ziomkowski ◽

João Rafael Silva Simões Estrela ◽

Nilo Jorge Carvalho Leão Barretto ◽

Nilo César Leão Barretto

Keyword(s):

Case Report ◽

Minimally Invasive ◽

Wilms Tumor ◽

Small Child ◽

Tumor Treatment ◽

Minimally Invasive Approach ◽

Invasive Approach

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Non-Linear Dynamics of Cardiovascular Variability Signals

Methods of Information in Medicine ◽

10.1055/s-0038-1634981 ◽

1994 ◽

Vol 33 (01) ◽

pp. 81-84 ◽

Cited By ~ 14

Author(s):

S. Cerutti ◽

S. Guzzetti ◽

R. Parola ◽

M.G. Signorini

Keyword(s):

Dimensional Space ◽

Severe Heart Failure ◽

Parametric Models ◽

Deterministic Systems ◽

Finite Dimensional ◽

Return Maps ◽

Pathological Conditions ◽

Non Linear ◽

Space State

Abstract:Long-term regulation of beat-to-beat variability involves several different kinds of controls. A linear approach performed by parametric models enhances the short-term regulation of the autonomic nervous system. Some non-linear long-term regulation can be assessed by the chaotic deterministic approach applied to the beat-to-beat variability of the discrete RR-interval series, extracted from the ECG. For chaotic deterministic systems, trajectories of the state vector describe a strange attractor characterized by a fractal of dimension D. Signals are supposed to be generated by a deterministic and finite dimensional but non-linear dynamic system with trajectories in a multi-dimensional space-state. We estimated the fractal dimension through the Grassberger and Procaccia algorithm and Self-Similarity approaches of the 24-h heart-rate variability (HRV) signal in different physiological and pathological conditions such as severe heart failure, or after heart transplantation. State-space representations through Return Maps are also obtained. Differences between physiological and pathological cases have been assessed and generally a decrease in the system complexity is correlated to pathological conditions.

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A Computerized Coding System for Processing Basic Histopathological Changes – Application to Vascular Pathology

Methods of Information in Medicine ◽

10.1055/s-0038-1635460 ◽

1986 ◽

Vol 25 (03) ◽

pp. 139-142 ◽

Cited By ~ 2

Author(s):

A. Mauriello ◽

Y. Sambuy ◽

E. Bonanno ◽

A. Orlandi ◽

G. Palmieri ◽

...

Keyword(s):

Vascular Wall ◽

Final Diagnosis ◽

Vascular Pathology ◽

Histopathological Study ◽

Coding System ◽

Histopathological Changes ◽

Histological Changes ◽

Pathological Conditions ◽

Computer Based ◽

Sufficient Space

SummaryAmong the numerous existing computer-based systems for processing pathological data, none contains sufficient space for encoding data on the basic cytological or histological changes of a certain organ or tissue, upon which the final diagnosis is based.An “analytical record” was constructed listing all the basic changes that can be encountered in the various pathological conditions of the vascular wall. The data collected on the “analytical record” were coded by means of an alphanumeric code and stored in an Apple II 48 K minicomputer.The advantages of this system include the computerization of the data by non-specialized personnel and the possibility to’ quantitatively analyze the histocytopathological parameters used for diagnosis in vascular pathology. This coding system may easily be adapted, with minor modifications, to the histopathological study of other organs and tissues.

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The Physician’s Task and the Problem of Documentation

Methods of Information in Medicine ◽

10.1055/s-0038-1636642 ◽

1964 ◽

Vol 03 (01) ◽

pp. 11-19 ◽

Cited By ~ 4

Author(s):

A. Proppe

Keyword(s):

Statistical Evaluation ◽

Age Groups ◽

Diagnostic Methods ◽

Early Age ◽

Case Histories ◽

Lupus Vulgaris ◽

Pathological Conditions ◽

Clinical Methods ◽

Corpus Hippocraticum ◽

High Degree

SummaryCase histories suitable for statistical evaluation can be found even as far back as in the Corpus Hippocraticum. Such simple data as the patient’s age, body weight, size, the date of menarche, etc. are practically always included in the case records, and it is demonstrated that, when such data are recorded in a system of documentation suitable for mechanical sorting, it may enable us to draw conclusions of very great importance. Mechanical registration methods have revealed that, in the determination and recording of data as hitherto carried out. there has been a surprisingly large number of errors and a high degree of unreliability. This view has a considerable influence on modern clinical methods; it renders a more democratic relation between physician and patient necessary and makes clear the need for measures to enhance the reliability of diagnosis and treatment of pathological conditions. The author illustrates this view with reference to the mechanical falsification of the thesis of the proneness of early age groups to lupus vulgaris, furthermore with reference to the mechanical rationalization of modern routine diagnostic methods, to the constant surveillance of adverse effects on public health and to the protection against allergic reactions with the aid of recording systems of personal allergy and intolerance data with mechanical sorting and computer techniques.

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Human Platelets Exposed to Mechanical Stresses Express a Potent Lipoxygenase Product

Thrombosis and Haemostasis ◽

10.1055/s-0038-1646322 ◽

1992 ◽

Vol 68 (05) ◽

pp. 589-594 ◽

Cited By ~ 9

Author(s):

Alon Margalit ◽

Avinoam A Livne

Keyword(s):

Regulatory Volume Decrease ◽

Human Platelets ◽

Mechanical Stresses ◽

Arterial Stenosis ◽

Platelet Suspension ◽

Suspension Flows ◽

Lipoxygenase Product ◽

Pathological Conditions ◽

K Permeability ◽

Volume Decrease

SummaryHuman platelets exposed to hypotonicity undergo regulatory volume decrease (RVD), controlled by a potent, yet labile, lipoxygenase product (LP). LP is synthesized and excreted during RVD affecting selectively K+ permeability. LP is assayed by its capacity to reconstitute RVD when lipoxygenase is blocked. Centrifugation for preparing washed platelets (1,550 × g, 10 min) is sufficient to express LP activity, with declining potency in repeated centrifugations, indicating that it is not readily replenish-able. When platelet suspension flows in a vinyl tubing (1 mm i.d.), at physiological velocity, controlled at 90–254 cm/s, LP formation increases as a function of velocity but declines as result of increasing the tubing length. Stirring the platelets in an aggregometer cuvette for 30 s, yields no LP unless the stirring is intermittent. No associated platelet lysis or aggregation are observed following the mechanical stress applications. These results demonstrate that although mechanical stresses result in LP production, the mode of its application plays a major role. These results may indicate that LP is synthesized under pathological conditions and could be of relevance to platelets behavior related to arterial stenosis.

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Contribution of large bacteria to bacterial biomass in a deep freshwater lake (Lake Biwa, Japan)

Aquatic Microbial Ecology ◽

10.3354/ame01949 ◽

2020 ◽

Vol 85 ◽

pp. 131-139

Author(s):

S Shen ◽

Y Shimizu

Keyword(s):

Cell Volume ◽

Bacterial Cell ◽

Lake Biwa ◽

Bacterial Biomass ◽

Spatial Variations ◽

Freshwater Lake ◽

Heterotrophic Nanoflagellates ◽

Bacterial Productivity ◽

Transmission Electron ◽

Seasonal And Spatial Variations

Despite the importance of bacterial cell volume in microbial ecology in aquatic environments, literature regarding the effects of seasonal and spatial variations on bacterial cell volume remains scarce. We used transmission electron microscopy to examine seasonal and spatial variations in bacterial cell size for 18 mo in 2 layers (epilimnion 0.5 m and hypolimnion 60 m) of Lake Biwa, Japan, a large and deep freshwater lake. During the stratified period, we found that the bacterial cell volume in the hypolimnion ranged from 0.017 to 0.12 µm3 (median), whereas that in the epilimnion was less variable (0.016 to 0.033 µm3, median) and much lower than that in the hypolimnion. Additionally, in the hypolimnion, cell volume during the stratified period was greater than that during the mixing period (up to 5.7-fold). These differences in cell volume resulted in comparable bacterial biomass in the hypolimnion and epilimnion, despite the fact that there was lower bacterial abundance in the hypolimnion than in the epilimnion. We also found that the biomass of larger bacteria, which are not likely to be grazed by heterotrophic nanoflagellates, increased in the hypolimnion during the stratified period. Our data suggest that estimation of carbon flux (e.g. bacterial productivity) needs to be interpreted cautiously when cell volume is used as a constant parametric value. In deep freshwater lakes, a difference in cell volume with seasonal and spatial variation may largely affect estimations.

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