Quorum sensing inhibitory effect of hexanal on Autoinducer‐2 (AI‐2) and corresponding impacts on biofilm formation and enzyme activity in Erwinia carotovora and Pseudomonas fluorescens isolated from vegetables

2022 ◽  
Author(s):  
Zhang Ying ◽  
Hang Yu ◽  
Yunfei Xie ◽  
Yahui Guo ◽  
Yuliang Cheng ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Quorum Sensing ◽  
Pseudomonas Fluorescens ◽  
Biofilm Formation ◽  
Erwinia Carotovora ◽  
Inhibitory Effect ◽  
Autoinducer 2

Essential oil components inhibit biofilm formation in Erwinia carotovora and Pseudomonas fluorescens via anti-quorum sensing activity

LWT ◽  
2018 ◽  
Vol 92 ◽  
pp. 133-139 ◽  
Author(s):  
Ying Zhang ◽  
Jie Kong ◽  
Yunfei Xie ◽  
Yahui Guo ◽  
Yuliang Cheng ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Essential Oil ◽  
Pseudomonas Fluorescens ◽  
Biofilm Formation ◽  
Erwinia Carotovora ◽  
Oil Components

scholarly journals Autoinducer-2 Triggers the Oxidative Stress Response in Mycobacterium avium, Leading to Biofilm Formation

2008 ◽  
Vol 74 (6) ◽  
pp. 1798-1804 ◽  
Author(s):  
Henriette Geier ◽  
Serge Mostowy ◽  
Gerard A. Cangelosi ◽  
Marcel A. Behr ◽  
Timothy E. Ford
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Quorum Sensing ◽  
Mycobacterium Avium ◽  
Biofilm Formation ◽  
Transcriptional Response ◽  
Opportunistic Pathogen ◽  
Environmental Stresses ◽  
Oxidative Stress Response ◽  
Autoinducer 2

ABSTRACT Mycobacterium avium is an environmental organism and opportunistic pathogen with inherent resistance to drugs, environmental stresses, and the host immune response. To adapt to these disparate conditions, M. avium must control its transcriptional response to environmental cues. M. avium forms biofilms in various environmental settings, including drinking water pipes and potable water reservoirs. In this study, we investigated the role of the universal signaling molecule autoinducer-2 (AI-2) in biofilm formation by M. avium. The addition of the compound to planktonic M. avium cultures resulted in increased biofilm formation. Microarray and reverse transcriptase PCR studies revealed an upregulation of the oxidative stress response upon addition of AI-2. This suggests that the response to AI-2 might be related to oxidative stress, rather than quorum sensing. Consistent with this model, addition of hydrogen peroxide, a known stimulus of the oxidative stress response, to M. avium cultures resulted in elevated biofilm formation. These results suggest that AI-2 does not act as a quorum-sensing signal in M. avium. Instead, biofilm formation is triggered by environmental stresses of biotic and abiotic origins and AI-2 may exert effects on that level.

Quorum sensing signal autoinducer-2 promotes root colonization of Bacillus velezensis SQR9 by affecting biofilm formation and motility

2020 ◽  
Vol 104 (16) ◽  
pp. 7177-7185
Author(s):  
Qin Xiong ◽  
Di Liu ◽  
Huihui Zhang ◽  
Xiaoyan Dong ◽  
Guishan Zhang ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Root Colonization ◽  
Bacillus Velezensis ◽  
Autoinducer 2

Quorum-sensing inhibition by hexanal in biofilms formed by Erwinia carotovora and Pseudomonas fluorescens

LWT ◽  
2019 ◽  
Vol 109 ◽  
pp. 145-152 ◽  
Author(s):  
Ying Zhang ◽  
Jie Kong ◽  
Yunfei Xie ◽  
Yahui Guo ◽  
Hang Yu ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Pseudomonas Fluorescens ◽  
Erwinia Carotovora ◽  
Quorum Sensing Inhibition

Effect of interspecies quorum sensing on the formation of aerobic granular sludge

2011 ◽  
Vol 64 (6) ◽  
pp. 1284-1290 ◽  
Author(s):  
Sheng-Hua Zhang ◽  
Xin Yu ◽  
Feng Guo ◽  
Zhuo-ying Wu
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Essential Element ◽  
Granular Sludge ◽  
Aerobic Granular Sludge ◽  
Signal Molecules ◽  
Small Signal ◽  
Autoinducer 2

Quorum sensing (QS) is a form of microbial communication that relies on small signal molecules to regulate group behaviors such as biofilm formation in response to population density. In this study, we attempted to apply the paradigm of bacterial QS to aerobic granular sludge (AGS) formation for wastewater treatment. An essential element of interspecies QS signals, boron, was added to a sequencing batch reactor (SBR) to stimulate AGS growth. Bioassays elaborated the activity of autoinducer-2 (AI-2). We found that boron accelerated AGS growth, resulting in improved settlement performance and increased biomass in the SBR. During continuous SBR operation, the AGS showed an obvious increase in AI-2 activity, which implies that interspecies QS was closely associated with AGS formation. Analysis of EPS showed that boron stimulated tryptophan production, and increased the hydrophobia of AGS. From these results, it was speculated that the addition of boron may have promoted the formation of boron complexed to (R)-4, 5-dihydroxy-2,3-pentanedione (DPD) as the precursor of AI-2, which resulted in accelerated interspecies QS. The results also suggested QS as a novel regulation target for the biogranulation process, such as AGS formation.

scholarly journals Role of the luxS Quorum-Sensing System in Biofilm Formation and Virulence of Staphylococcus epidermidis

2006 ◽  
Vol 74 (1) ◽  
pp. 488-496 ◽  
Author(s):  
Lin Xu ◽  
Hualin Li ◽  
Cuong Vuong ◽  
Viveka Vadyvaloo ◽  
Jianping Wang ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Mutant Strain ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Bacterial Pathogens ◽  
Sensing System ◽  
Autoinducer 2 ◽  
Enhanced Production ◽  
Quorum Sensing System ◽  
Biofilm Development

ABSTRACT Nosocomial infections caused by Staphylococcus epidermidis are characterized by biofilm formation on implanted medical devices. Quorum-sensing regulation plays a major role in the biofilm development of many bacterial pathogens. Here, we describe luxS, a quorum-sensing system in staphylococci that has a significant impact on biofilm development and virulence. We constructed an isogenic ΔluxS mutant strain of a biofilm-forming clinical isolate of S. epidermidis and demonstrated that luxS signaling is functional in S. epidermidis. The mutant strain showed increased biofilm formation in vitro and enhanced virulence in a rat model of biofilm-associated infection. Genetic complementation and addition of autoinducer 2-containing culture filtrate restored the wild-type phenotype, demonstrating that luxS repressed biofilm formation through a cell-cell signaling mechanism based on autoinducer 2 secretion. Enhanced production of the biofilm exopolysaccharide polysaccharide intercellular adhesin in the mutant strain is presumably the major cause of the observed phenotype. The agr quorum-sensing system has previously been shown to impact biofilm development and biofilm-associated infection in a way similar to that of luxS, although by regulation of different factors. Our study indicates a general scheme of quorum-sensing regulation of biofilm development in staphylococci, which contrasts with that observed in many other bacterial pathogens.

scholarly journals Inhibition of AI-2 Quorum Sensing and Biofilm Formation in Campylobacter jejuni by Decanoic and Lauric Acids

2022 ◽  
Vol 12 ◽  
Author(s):  
Shenmiao Li ◽  
Kelvin Ka-wan Chan ◽  
Marti Z. Hua ◽  
Greta Gölz ◽  
Xiaonan Lu
Keyword(s):  
Fatty Acids ◽  
Quorum Sensing ◽  
Campylobacter Jejuni ◽  
Lauric Acid ◽  
Biofilm Formation ◽  
Control Strategies ◽  
Decanoic Acid ◽  
Poultry Production ◽  
Bacterial Inactivation ◽  
Autoinducer 2

Campylobacter jejuni is a major bacterial cause of human diarrheal diseases worldwide. Despite its sensitivity to environmental stresses, C. jejuni ubiquitously distributes throughout poultry production chains. Biofilm formation mediated by quorum sensing is suggested to be critical to the survival of C. jejuni in agroecosystem. C. jejuni possesses LuxS, the enzyme involved in the production of autoinducer-2 (AI-2) signaling molecules. In this study, two fatty acids, namely decanoic acid and lauric acid, were identified to be effective in inhibiting AI-2 activity of C. jejuni. Both decanoic acid and lauric acid at 100 ppm inhibited ∼90% AI-2 activity (P < 0.05) of C. jejuni without bacterial inactivation. The biofilm biomass of two C. jejuni strains was reduced by 10–50% (P < 0.05) after treatment by both fatty acids, while increased biofilm formation was observed for one C. jejuni strain. In addition, both fatty acids effectively reduced the motility of all tested C. jejuni strains. These findings can aid in developing alternative C. jejuni control strategies in agri-food and clinical settings.

scholarly journals Autoinducer 2 Affects Biofilm Formation by Bacillus cereus

2006 ◽  
Vol 72 (1) ◽  
pp. 937-941 ◽  
Author(s):  
Sandrine Auger ◽  
Evelyne Krin ◽  
Stéphane Aymerich ◽  
Michel Gohar
Keyword(s):  
Bacillus Cereus ◽  
Biofilm Formation ◽  
Inhibitory Effect ◽  
Strain Atcc ◽  
Photorhabdus Luminescens ◽  
Autoinducer 2 ◽  
Exogenous Addition

ABSTRACT Cell-free supernatants from growing Bacillus cereus strain ATCC 10987 induced luminescence in a Photorhabdus luminescens ΔluxS mutant, indicating the production of functional autoinducer 2 (AI-2). The exogenous addition of in vitro synthesized AI-2 had an inhibitory effect on biofilm formation by B. cereus and promoted release of the cells from a preformed biofilm.

Sign in / Sign up

Export Citation Format

Share Document