scholarly journals Unlike canonical bacterial models for cell division, cyanobacteria possess extensive thylakoid membranes. In the cyanobacterium Synechococcus elongatus PCC 7942, MinCD regulates FtsZ positioning via dynamic oscillations that require thylakoid membrane perforations for access of MinD and MinE to the plasma membrane. For details see the article by MacCready et al . on pp. 483–503 of this issue.

2017 ◽  
Vol 103 (3) ◽  
Keyword(s):  
Plasma Membrane ◽  
Cell Division ◽  
Thylakoid Membrane ◽  
Thylakoid Membranes ◽  
Synechococcus Elongatus ◽  
Synechococcus Elongatus Pcc 7942 ◽  
Pcc 7942

Complete Replacement of the Galactolipid Biosynthesis Pathway with a Plant-Type Pathway in the Cyanobacterium Synechococcus elongatus PCC 7942

2020 ◽  
Vol 61 (9) ◽  
pp. 1661-1668
Author(s):  
Egi Tritya Apdila ◽  
Shukumi Inoue ◽  
Mie Shimojima ◽  
Koichiro Awai
Keyword(s):  
Chlorophyll Content ◽  
Membrane Lipids ◽  
Thylakoid Membranes ◽  
Synechococcus Elongatus ◽  
Biosynthesis Pathway ◽  
Plant Type ◽  
Complete Replacement ◽  
Essential Components ◽  
Synechococcus Elongatus Pcc 7942 ◽  
Pcc 7942

Abstract Monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) are the major components of thylakoid membranes and well-conserved from cyanobacteria to chloroplasts. However, cyanobacteria and chloroplasts synthesize these galactolipids using different pathways and enzymes, but they are believed to share a common ancestor. This fact implies that there was a replacement of the cyanobacterial galactolipid biosynthesis pathway during the evolution of a chloroplast. In this study, we first replaced the cyanobacterial MGDG biosynthesis pathway in a model cyanobacterium, Synechococcus elongatus PCC 7942, with the corresponding plant-type pathway. No obvious phenotype was observed under the optimum growth condition, and the content of membrane lipids was not largely altered in the transformants. We next replaced the cyanobacterial DGDG biosynthesis pathway with the corresponding plant-type pathway using the strain described above and isolated the strain harboring the replaced plant-type pathway instead of the whole galactolipid biosynthesis pathway. This transformant, SeGPT, can grow photoautotrophically, indicating that cyanobacterial galactolipid biosynthesis pathways can be functionally complemented by the corresponding plant-type pathways and that the lipid products MGDG and DGDG, and not biosynthesis pathways, are important. While SeGPT does not show strong growth retardation, the strain has low cellular chlorophyll content but it retained a similar oxygen evolution rate per chlorophyll content compared with the wild type. An increase in total membrane lipid content was observed in SeGPT, which was caused by a significant increase in DGDG content. SeGPT accumulated carotenoids from the xanthophyll groups. These results suggest that cyanobacteria have the capacity to accept other pathways to synthesize essential components of thylakoid membranes.

scholarly journals Probing the biogenesis pathway and dynamics of thylakoid membranes

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Tuomas Huokko ◽  
Tao Ni ◽  
Gregory F. Dykes ◽  
Deborah M. Simpson ◽  
Philip Brownridge ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Photosystem I ◽  
Spatial Organization ◽  
Electron Flux ◽  
Protein Complexes ◽  
Thylakoid Membranes ◽  
Thylakoid Biogenesis ◽  
Photosynthetic Complexes ◽  
Photosynthetic Machinery ◽  
Pcc 7942

AbstractHow thylakoid membranes are generated to form a metabolically active membrane network and how thylakoid membranes orchestrate the insertion and localization of protein complexes for efficient electron flux remain elusive. Here, we develop a method to modulate thylakoid biogenesis in the rod-shaped cyanobacterium Synechococcus elongatus PCC 7942 by modulating light intensity during cell growth, and probe the spatial-temporal stepwise biogenesis process of thylakoid membranes in cells. Our results reveal that the plasma membrane and regularly arranged concentric thylakoid layers have no physical connections. The newly synthesized thylakoid membrane fragments emerge between the plasma membrane and pre-existing thylakoids. Photosystem I monomers appear in the thylakoid membranes earlier than other mature photosystem assemblies, followed by generation of Photosystem I trimers and Photosystem II complexes. Redistribution of photosynthetic complexes during thylakoid biogenesis ensures establishment of the spatial organization of the functional thylakoid network. This study provides insights into the dynamic biogenesis process and maturation of the functional photosynthetic machinery.

scholarly journals Photophysiological and Photosynthetic Complex Changes during Iron Starvation in Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e59861 ◽  
Author(s):  
Jared M. Fraser ◽  
Sarah E. Tulk ◽  
Jennifer A. Jeans ◽  
Douglas A. Campbell ◽  
Thomas S. Bibby ◽  
...  
Keyword(s):  
Synechococcus Elongatus ◽  
Iron Starvation ◽  
Synechococcus Elongatus Pcc 7942 ◽  
Pcc 7942 ◽  
Synechocystis Sp ◽  
Pcc 6803

Stability of the Synechococcus elongatus PCC 7942 circadian clock under directed anti-phase expression of the kai genes

Microbiology ◽  
2005 ◽  
Vol 151 (8) ◽  
pp. 2605-2613 ◽  
Author(s):  
Jayna L. Ditty ◽  
Shannon R. Canales ◽  
Breanne E. Anderson ◽  
Stanly B. Williams ◽  
Susan S. Golden
Keyword(s):  
Circadian Clock ◽  
Expression Patterns ◽  
Phase Relationship ◽  
Synechococcus Elongatus ◽  
Cycle Period ◽  
Rhythmic Expression ◽  
Core Components ◽  
Synechococcus Elongatus Pcc 7942 ◽  
Time Required ◽  
Pcc 7942

The kaiA, kaiB and kaiC genes encode the core components of the cyanobacterial circadian clock in Synechococcus elongatus PCC 7942. Rhythmic expression patterns of kaiA and of the kaiBC operon normally peak in synchrony. In some mutants the relative timing of peaks (phase relationship) between these transcription units is altered, but circadian rhythms persist robustly. In this study, the importance of the transcriptional timing of kai genes was examined. Expressing either kaiA or kaiBC from a heterologous promoter whose peak expression occurs 12 h out of phase from the norm, and thus 12 h out of phase from the other kai locus, did not affect the time required for one cycle (period) or phase of the circadian rhythm, as measured by bioluminescence reporters. Furthermore, the data confirm that specific cis elements within the promoters of the kai genes are not necessary to sustain clock function.

scholarly journals Putative extracellular α-class carbonic anhydrase, EcaA, of Synechococcus elongatus PCC 7942 is an active enzyme: a sequel to an old story

Microbiology ◽  
2018 ◽  
Vol 164 (4) ◽  
pp. 576-586 ◽  
Author(s):  
Elena V. Kupriyanova ◽  
Maria A. Sinetova ◽  
Vladimir S. Bedbenov ◽  
Natalia A. Pronina ◽  
Dmitry A. Los
Keyword(s):  
Carbonic Anhydrase ◽  
Synechococcus Elongatus ◽  
Active Enzyme ◽  
Synechococcus Elongatus Pcc 7942 ◽  
Pcc 7942

scholarly journals Role of KaiC phosphorylation in the circadian clock system of Synechococcus elongatus PCC 7942

2004 ◽  
Vol 101 (38) ◽  
pp. 13927-13932 ◽  
Author(s):  
T. Nishiwaki ◽  
Y. Satomi ◽  
M. Nakajima ◽  
C. Lee ◽  
R. Kiyohara ◽  
...  
Keyword(s):  
Circadian Clock ◽  
Synechococcus Elongatus ◽  
Clock System ◽  
Synechococcus Elongatus Pcc 7942 ◽  
Pcc 7942

scholarly journals Functional characterisation of the peroxiredoxin gene family members of Synechococcus elongatus PCC 7942

2008 ◽  
Vol 191 (2) ◽  
pp. 141-151 ◽  
Author(s):  
Tina Stork ◽  
Miriam Laxa ◽  
Marina S. Dietz ◽  
Karl-Josef Dietz
Keyword(s):  
Gene Family ◽  
Family Members ◽  
Synechococcus Elongatus ◽  
Functional Characterisation ◽  
Synechococcus Elongatus Pcc 7942 ◽  
Pcc 7942
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