Allelic differences of clustered terpene synthases contribute to correlated intraspecific variation of floral and herbivory‐induced volatiles in a wild tobacco

Abstract Plants not only respond to herbivorous damage but adjust their defense system after egg deposition by pest insects. Thereby, parasitoids use oviposition-induced plant volatiles to locate their hosts. We investigated the olfactory behavioral responses of Trichogramma pretiosum Riley, 1879 (Hymenoptera: Trichogrammatidae) to volatile blends emitted by maize (Zea mays L.) with singular and stacked events after oviposition by Spodoptera frugiperda Smith, 1797 (Hymenoptera: Trichogrammatidae) moths. Additionally, we examined possible variations in gene expression and on oviposition-induced volatiles. We used a Y-tube olfactometer to test for the wasp responses to volatiles released by maize plants oviposited by S. frugiperda and not-oviposited plants. Using the real-time PCR technique (qRT-PCR), we analyzed the expression of lipoxygenase and three terpene synthases genes, which are enzymes involved in the synthesis of volatile compounds that attract parasitoids of S. frugiperda. Olfactometer tests showed that T. pretiosum is strongly attracted by volatiles from transgenic maize emitted by S. frugiperda oviposition (VTPRO 3, more than 75% individuals were attracted). The relative expression of genes TPS10, LOX e STC was higher in transgenic hybrids than in the conventional (isogenic line) hybrids. The GC-MS analysis revealed that some volatile compounds are released exclusively by transgenic maize. This study provides evidence that transgenic hybrids enhanced chemical cues under oviposition-induction and helped to increase T. pretiosum efficiency in S. frugiperda control. This finding shows that among the evaluated hybrids, genetically modified hybrids can improve the biological control programs, since they potentialize the egg parasitoid foraging, integrating pest management.

Download Full-text

Faculty Opinions recommendation of The ecological importance of intraspecific variation.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.732227334.793578012 ◽

2020 ◽

Author(s):

Nelson Hairston Jr

Keyword(s):

Intraspecific Variation ◽

Ecological Importance

Download Full-text

Complete Chloroplast Genome of Japanese Larch (Larix kaempferi): Insights into Intraspecific Variation with an Isolated Northern Limit Population

Forests ◽

10.3390/f11080884 ◽

2020 ◽

Vol 11 (8) ◽

pp. 884

Author(s):

Shufen Chen ◽

Wataru Ishizuka ◽

Toshihiko Hara ◽

Susumu Goto

Keyword(s):

Chloroplast Genome ◽

Intraspecific Variation ◽

Japanese Larch ◽

Monophyletic Group ◽

Phylogenetic Position ◽

Larix Kaempferi ◽

Northern Limit ◽

Complete Chloroplast Genome ◽

Intraspecific Variations ◽

Cpdna Markers

Research Highlights: The complete chloroplast genome for eight individuals of Japanese larch, including from the isolated population at the northern limit of the range (Manokami larch), revealed that Japanese larch forms a monophyletic group, within which Manokami larch can be phylogenetically placed in Japanese larch. We detected intraspecific variation for possible candidate cpDNA markers in Japanese larch. Background and Objectives: The natural distribution of Japanese larch is limited to the mountainous range in the central part of Honshu Island, Japan, with an isolated northern limit population (Manokami larch). In this study, we determined the phylogenetic position of Manokami larch within Japanese larch, characterized the chloroplast genome of Japanese larch, detected intraspecific variation, and determined candidate cpDNA markers. Materials and Methods: The complete genome sequence was determined for eight individuals, including Manokami larch, in this study. The genetic position of the northern limit population was evaluated using phylogenetic analysis. The chloroplast genome of Japanese larch was characterized by comparison with eight individuals. Furthermore, intraspecific variations were extracted to find candidate cpDNA markers. Results: The phylogenetic tree showed that Japanese larch forms a monophyletic group, within which Manokami larch can be phylogenetically placed, based on the complete chloroplast genome, with a bootstrap value of 100%. The value of nucleotide diversity (π) was calculated at 0.00004, based on SNP sites for Japanese larch, suggesting that sequences had low variation. However, we found three hyper-polymorphic regions within the cpDNA. Finally, we detected 31 intraspecific variations, including 19 single nucleotide polymorphisms, 8 simple sequence repeats, and 4 insertions or deletions. Conclusions: Using a distant genotype in a northern limit population (Manokami larch), we detected sufficient intraspecific variation for the possible candidates of cpDNA markers in Japanese larch.

Download Full-text

Variation of the human mu-opioid receptor (OPRM1) gene predicts vulnerability to frustration

Scientific Reports ◽

10.1038/s41598-020-78783-4 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Alan M. Daniel ◽

Brenda G. Rushing ◽

Karla Y. Tapia Menchaca

Keyword(s):

Individual Differences ◽

Animal Models ◽

Opioid Receptor ◽

Receptor Gene ◽

Emotional Reaction ◽

Mu Opioid Receptor ◽

Reward Processing ◽

Physical Pain ◽

Mu Opioid ◽

Allelic Differences

AbstractUnderstanding the emotional reaction to loss, or frustration, is a critical problem for the field of mental health. Animal models of loss have pointed to the opioid system as a nexus of frustration, physical pain, and substance abuse. However, few attempts have been made to connect the results of animal models of loss to human behavior. Allelic differences in the human mu opioid receptor gene, notably the A118G single nucleotide polymorphism, have been linked to individual differences in pain sensitivity, depressive symptoms, and reward processing. The present study explored the relationship between A118G and behavior in two frustrating tasks in humans. Results showed that carriers of the mutant G-allele were slower to recover behavior following a reward downshift and abandoned a frustrating task earlier than those without the mutation. Additionally, G-carriers were more sensitive to physical pain. These results highlight the overlap between frustration and pain, and suggest that genetic variation in opioid tone may contribute to individual differences in vulnerability and resilience following emotional disturbances.

Download Full-text

Effects of heavy metal-mediated intraspecific variation in leaf litter on the feeding preferences of stream detritivores

The Science of The Total Environment ◽

10.1016/j.scitotenv.2020.144591 ◽

2021 ◽

Vol 763 ◽

pp. 144591

Author(s):

Run Liu ◽

Yueting Pan ◽

You Fang ◽

Lu Pang ◽

Jiachen Shen ◽

...

Keyword(s):

Heavy Metal ◽

Leaf Litter ◽

Intraspecific Variation ◽

Feeding Preferences

Download Full-text

Intraspecific variation in the Cambrian: new observations on the morphology of the Chengjiang euarthropod Sinoburius lunaris

BMC Ecology and Evolution ◽

10.1186/s12862-021-01854-1 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Michel Schmidt ◽

Yu Liu ◽

Xianguang Hou ◽

Joachim T. Haug ◽

Carolin Haug ◽

...

Keyword(s):

Intraspecific Variation ◽

Southwest China ◽

3D Models ◽

Morphological Characters ◽

Open Questions ◽

New Findings ◽

Unique Shape ◽

Variable Species ◽

Chengjiang Biota ◽

3D Software

Abstract Background The Chengjiang biota from southwest China (518-million-years old, early Cambrian) has yielded nearly 300 species, of which more than 80 species represent early chelicerates, crustaceans and relatives. The application of µCT-techniques combined with 3D software (e.g., Drishti), has been shown to be a powerful tool in revealing and analyzing 3D features of the Chengjiang euarthropods. In order to address several open questions that remained from previous studies on the morphology of the xandarellid euarthropod Sinoburius lunaris, we reinvestigated the µCT data with Amira to obtain a different approach of visualization and to generate new volume-rendered models. Furthermore, we used Blender to design 3D models showing aspects of intraspecific variation. Results New findings are: (1) antennulae consist of additional proximal articles that have not been detected before; (2) compared to other appendages, the second post-antennular appendage has a unique shape, and its endopod is comprised of only five articles (instead of seven); (3) the pygidium bears four pairs of appendages which are observed in all specimens. On the other hand, differences between specimens also have been detected. These include the presence/absence of diplotergites resulting in different numbers of post-antennular appendages and tergites and different distances between the tip of the hypostome and the anterior margin of the head shield. Conclusions Those new observations reveal intraspecific variation among Chengjiang euarthropods not observed before and encourage considerations about possible sexual dimorphic pairs or ontogenetic stages. Sinoburius lunaris is a variable species with respect to its morphological characters, cautioning that taxon-specific variabilities need to be considered when exploring new species.

Download Full-text

Identification ofvib-1, a Locus Involved in Vegetative Incompatibility Mediated byhet-cinNeurospora crassa

Genetics ◽

10.1093/genetics/162.1.89 ◽

2002 ◽

Vol 162 (1) ◽

pp. 89-101 ◽

Cited By ~ 6

Author(s):

Qijun Xiang ◽

N Louise Glass

Keyword(s):

Acid Phosphatase ◽

Recognition System ◽

Deletion Mutants ◽

Wild Type ◽

Vegetative Incompatibility ◽

Death Rates ◽

Self Recognition ◽

Allelic Differences ◽

Native Gel ◽

Type Strains

AbstractA non-self-recognition system called vegetative incompatibility is ubiquitous in filamentous fungi and is genetically regulated by het loci. Different fungal individuals are unable to form viable heterokaryons if they differ in allelic specificity at a het locus. To identify components of vegetative incompatibility mediated by allelic differences at the het-c locus of Neurospora crassa, we isolated mutants that suppressed phenotypic aspects of het-c vegetative incompatibility. Three deletion mutants were identified; the deletions overlapped each other in an ORF named vib-1 (vegetative incompatibility blocked). Mutations in vib-1 fully relieved growth inhibition and repression of conidiation conferred by het-c vegetative incompatibility and significantly reduced hyphal compartmentation and death rates. The vib-1 mutants displayed a profuse conidiation pattern, suggesting that VIB-1 is a regulator of conidiation. VIB-1 shares a region of similarity to PHOG, a possible phosphate nonrepressible acid phosphatase in Aspergillus nidulans. Native gel analysis of wild-type strains and vib-1 mutants indicated that vib-1 is not the structural gene for nonrepressible acid phosphatase, but rather may regulate nonrepressible acid phosphatase activity.

Download Full-text

New femoral remains of Nacholapithecus kerioi: Implications for intraspecific variation and Miocene hominoid evolution

Journal of Human Evolution ◽

10.1016/j.jhevol.2021.102982 ◽

2021 ◽

Vol 155 ◽

pp. 102982

Author(s):

Marta Pina ◽

Yasuhiro Kikuchi ◽

Masato Nakatsukasa ◽

Yoshihiko Nakano ◽

Yutaka Kunimatsu ◽

...

Keyword(s):

Intraspecific Variation ◽

Hominoid Evolution

Download Full-text

Metabolic traits in brown trout (Salmo trutta) vary in response to food restriction and intrinsic factors

Conservation Physiology ◽

10.1093/conphys/coaa096 ◽

2020 ◽

Vol 8 (1) ◽

Author(s):

Louise C Archer ◽

Stephen A Hutton ◽

Luke Harman ◽

W Russell Poole ◽

Patrick Gargan ◽

...

Keyword(s):

Life History ◽

Metabolic Rate ◽

Intraspecific Variation ◽

Brown Trout ◽

Environmental Conditions ◽

Food Restriction ◽

Metabolic Rates ◽

Intrinsic Factors ◽

Metabolic Traits ◽

Food Conditions

Abstract Metabolic rates vary hugely within and between populations, yet we know relatively little about factors causing intraspecific variation. Since metabolic rate determines the energetic cost of life, uncovering these sources of variation is important to understand and forecast responses to environmental change. Moreover, few studies have examined factors causing intraspecific variation in metabolic flexibility. We explore how extrinsic environmental conditions and intrinsic factors contribute to variation in metabolic traits in brown trout, an iconic and polymorphic species that is threatened across much of its native range. We measured metabolic traits in offspring from two wild populations that naturally show life-history variation in migratory tactics (one anadromous, i.e. sea-migratory, one non-anadromous) that we reared under either optimal food or experimental conditions of long-term food restriction (lasting between 7 and 17 months). Both populations showed decreased standard metabolic rates (SMR—baseline energy requirements) under low food conditions. The anadromous population had higher maximum metabolic rate (MMR) than the non-anadromous population, and marginally higher SMR. The MMR difference was greater than SMR and consequently aerobic scope (AS) was higher in the anadromous population. MMR and AS were both higher in males than females. The anadromous population also had higher AS under low food compared to optimal food conditions, consistent with population-specific effects of food restriction on AS. Our results suggest different components of metabolic rate can vary in their response to environmental conditions, and according to intrinsic (population-background/sex) effects. Populations might further differ in their flexibility of metabolic traits, potentially due to intrinsic factors related to life history (e.g. migratory tactics). More comparisons of populations/individuals with divergent life histories will help to reveal this. Overall, our study suggests that incorporating an understanding of metabolic trait variation and flexibility and linking this to life history and demography will improve our ability to conserve populations experiencing global change.

Download Full-text