ANALYSIS OF GAMMA IRRADIATED FOURTH GENERATION MUTANT OF RODENT TUBER (TYPHONIUM FLAGELLIFORME LODD.) BASED ON MORPHOLOGY AND RAPD MARKERS

2016 ◽  
Vol 78 (5-6) ◽  
Author(s):  
Nesti Fronika Sianipar ◽  
Ragapadmi Purnamaningsih ◽  
Devie Lolita Gumanti ◽  
Rosaria Rosaria ◽  
Merry Vidianty
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Gamma Ray ◽  
Dry Weight ◽  
Genetic Mutation ◽  
The Other ◽  
Fourth Generation ◽  
Genetic Profile ◽  
Polymorphic Bands ◽  
Putative Mutant

Rodent tuber (Typhonium flagelliforme Lodd.) is an herbal medicinal plant with anticancer activity. The genetic diversity of rodent tuber is low due to vegetative propagation. Somatic cell population of rodent tuber from Bogor had been irradiated with gamma ray to increase genetic diversity. There were 37 clones of first generation putative mutant (MV1) which had been analyzed based on morphological and RAPD markers. Out of those 37 MV1 clones, there were 17 clones which had undergone genetic mutation and had a diversified genetic profile. MV1 had been regenerated to fourth generation putative mutant clones (MV4). This research was aimed to analyze the mutation stability of MV4 based on morphological and RAPD markers. Clone 6-1-2 had the highest increase of the number of shoots and leaves than control and the other MV4 clones, with 4.7 and 19.7 shoots and leaves, respectively. Clone 6-1-1-6 obtained the highest increase in plant height than control and the other MV4 clones, i.e. 25.2 cm. Clone 6-9-5 had the weightiest fresh and dry weight, i.e. 41.67 gram and 12.01 gram respectively. RAPD molecular marker analysis of MV4 by using 15 primers had produced 64 polymorphic DNA bands out of 146 total bands. OPD-10 primer produced the highest number of polymorphic bands, i.e. 15 polymorphic bands out of 17 total bands with sizes 200-2000 bp. RAPD profile of MV4 had showed 5 main clusters at similiarity coefficient cut-off 0.91. Morphological characterization and RAPD analysis had proved the stability of genetic mutation of MV4.

scholarly journals Evaluation of Potato Cultivars Grown in Saudi Arabia by Morphological Characters and RAPD Markers

2007 ◽  
Vol 12 ◽  
pp. 35
Author(s):  
Abd-Rahman Mohamed M. Al-Moshileh ◽  
Mohamed Zaky El-Shinawy ◽  
Mohamed Ibrahim Motawei
Keyword(s):  
Genetic Diversity ◽  
Genetic Similarity ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Dry Weight ◽  
Morphological Characters ◽  
Potato Cultivars ◽  
The Other ◽  
Polymerase Chain ◽  
Pair Wise Comparison

Genetic diversity of 10 potato cultivars was investigated at the DNA level with the random amplified polymorphic DNA (RAPD) procedure and at the phenotypic level using morphological characters. The results indicated that there were considerable variations among the different studied cultivars. Cultivars Victoria, Frizia and Safaren had the highest chlorophyll content compared to the other cultivars in both seasons. The largest leaf area and leaf dry weight were measured in cultivar Safaren and Mondial in both seasons. Cultivars Aboulx and Mondia produced the highest tuber yield in both seasons. On the other hand, cultivars Victoria and Edward were characterized by their considerably lower yield than other studied cultivars. Specific gravity varied considerably among the different potato cultivars. Thirteen random decamer primers were used to amplify DNA via the polymerase chain reaction and 75 RAPDs were generated. The RAPD profiles obtained were successfully used to differentiate potato cultivars. Based on the pair-wise comparison of amplification products, genetic similarity was estimated. The genetic similarity among all potato cultivars ranged from 50 to 92 %. Cultivars Victoria and Etfadoal presented the least similarity (0.50) while cultivars Mondial and Citrix had the greatest similarity (0.92). Etfadoal cultivar displayed the greatest genetic diversity of all cultivars. A dendrogram was constructed using UPGMA analysis. On the basis of this analysis, the cultivars were grouped into three clusters. The polymorphism detected suggests that RAPD markers are reliable for identification of potato cultivars and could be exploited in genetic mapping of populations to tag economically important traits. 

Evaluation of Genetic Diversity in Castor (Ricinus communis L.) using RAPD Markes

2011 ◽  
Vol 343-344 ◽  
pp. 981-987
Author(s):  
Feng Juan Li ◽  
Chang Lu Wang ◽  
Dong He ◽  
Ya Qiong Liu ◽  
Mian Hua Chen ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Tree ◽  
Genetic Distance ◽  
Oil Content ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Ricinus Communis ◽  
Major Group ◽  
Ricinus Communis L ◽  
Polymorphic Bands

RAPD markers are used to study the genetic diversity of the main planting on 37 castor varieties widely cultivated in china according to the oil content and other characteristic of different castor varieties. Genetic distance of 37 Chinese castor varieties is studied by RAPD markers analysis. RAPD analysis shows that a total of 122 bands are amplified from random primers of 20 S series, including 71 polymorphic bands with polymorphic rate of 58.20%. 37 castor beans are divided into four major groups in the phylogenetic tree. One castor germplasm is included in1, 2, 3 groups respectively, and two sub-groups are included in the 4 major group.

scholarly journals Genetic diversity among Asparagus species using morphological characteristics and RAPD markers in Pakistan

2019 ◽  
Vol 5 (1) ◽  
pp. 13-24
Author(s):  
M Irshad ◽  
M Idrees ◽  
A Tariq ◽  
ML Pathak ◽  
M Hanif ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Field Experiments ◽  
Morphological Characteristics ◽  
Dry Weight ◽  
Phenotypic Variance ◽  
Neighbor Joining ◽  
Comparative Performance ◽  
Shoot Height

The aim of present research was to study the genetic diversity among Asparagus species and its cultivars using morphological characteristics and RAPD markers. In-vitro and field experiments of 14 germplasm sources of Asparagus species and its cultivars were conducted at the Kohat University of Science and Technology to estimate the comparative performance. Highest genotypic variance, phenotypic variance, genotypic and phenotypic coefficient of variance were observed in shoot height in shoot related traits were 318.40, 320.30, 939.34 and 944.96, respectively, whereas highest values for root related traits were observed in root length that were 21.84, 22.35, 141.60 and 144.91 respectively. Among the shoot related traits maximum heritability, genetic advance and genetic gain were calculated for shoot length, while in root related traits these characters were highest for root dry weight. RAPD markers were used to evaluate genetic diversity analysis of 14 germplasm sources of Asparagus species and its cultivars. RAPD markers generated a total of 247 bands, of these 239 bands were polymorphic with average of 34.1 bands per primer. Cluster analysis based on Neighbor Joining methods showed that wild species (A. adsendens, A. densiflorus, A. capitatus, A. gracelus, A. plumosus, A. racemosus and A. setaceus) were genetically distant from A. officinalis and its cultivars (Abril, Apollo, Gersengum, Huchel, Para seletion and Taranga). The results of the present investigations could be particularly used for authentic identification and would be useful for evaluation of genetic improvement of Asparagus species and its cultivars. J. Biodivers. Conserv. Bioresour. Manag. 2019, 5(1): 13-24

Short Communication: Genetic variation of Coelogyne pandurate, C. rumphii and their hybrids based on RAPD markers

2020 ◽  
Vol 21 (10) ◽  
Author(s):  
Sri Hartati ◽  
ENDANG S. MULIAWATI
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Rapd Markers ◽  
Short Communication ◽  
F1 Hybrids ◽  
Polymorphic Bands ◽  
Large Clusters

Abstract. Hartati S, Muliawati ES. 2020. Short Communication: Genetic variation of Coelogyne pandurata, C. rumphii and their hybrids based on RAPD markers. Biodiversitas 21: 4709-4713. One effort to increase the genetic diversity of orchids is by crossing. This research aims to assess the genetic variation of a hybrid orchid obtained by crossing Coelogyne pandurata and C. rumphii and their hybrids based on RAPD markers. In this research, both parents were analyzed in three replications, while the hybrid was done in 10 replications. The study was conducted by analyzing DNA bands using RAPD markers with six primers, i.e. OPA 02, OPA 07, OPA 13, OPB 12, OPB 17, and OPD 08. Identification of the parents and their F1 hybrids showed 95.83% polymorphic bands with 43 bands measuring 200-2100 bp. The parents of C. rumphii, C. pandurata, and their hybrids showed similarity range of 0.16-1.00. The crossing of C. rumphii and C. pandurata resulted in a similarity of 0.5, shown in two large clusters. The first cluster consisted only of C. pandurata males and the second cluster consisted of C. rumphii females, together with all hybrid individuals. This study succeeded in creating new hybrids of orchids that have different characters from their parents, having a genetic variation of 23%.

scholarly journals Genetic diversity and productivity of Gracilaria coronopifolia as alternative for food resource based on RAPD marker

2019 ◽  
Vol 20 (12) ◽  
Author(s):  
Gut Windarsih ◽  
DWINITA WIKAN UTAMI ◽  
SITI YURIYAH
Keyword(s):  
Genetic Diversity ◽  
Rapd Marker ◽  
Food Resource ◽  
Dry Weight ◽  
Selection Marker ◽  
Genetic Profile ◽  
High Productivity ◽  
Band Size ◽  
Three Samples ◽  
Group B

Abstract. Windarsih G, Utami DW, Yuriyah S. 2019. Genetic diversity and productivity of Gracilaria coronopifolia as alternative for food resource based on RAPD marker. Biodiversitas 20: 3758-3765. Gracilaria coronopifolia is one of potential seaweed as an alternative for food resources, especially for agar source. The information about the genetic profile of G. coronopifolia will be useful for assisting in conservation and breeding efforts. RAPD marker is expected to be utilized for characterization of the species with high productivity based on genetic profile. This research aims to determine the genetic diversity and productivity of G. coronopifolia based on RAPD marker. The samples were collected from Anyer Beach, Banten, Indonesia i.e. Tanjung Tum Beach, Mambruk Hotel Beach, and Patra Comfort Anyer Beach, as well as the cultivation ponds of seaweed in Lontar Village, Banten, Indonesia. Five RAPD primers (OPA-01, OPA-16, OPT-05, OPR-02, and UBC-594) were screened to characterize the genetic profile of samples. The results showed that all primers produced polymorphic bands. Based on the genetic profile, the samples of G. coronopifolia were separated into two main clusters, consisted of group A (Tanjung Tum Beach, Patra Beach, and Mambruk Beach) with a coefficient of similarity of 0.44 and group B (Lontar Village) with a similarity coefficient of 0.43. The sample from Lontar Village was separated outside from the other three samples at the waters of Anyer Beach. The sample from Lontar Village had the highest talus height (18.0 cm) and dry weight (1.250 g/clump). The UBC-594 primer was the most associated with the talus height and dry weight on the band size of 50 bp, thus this primer was assumed can be used as a selection marker for the talus height and dry weight of G. coronopifolia.

scholarly journals Genetic Relationship Among Ten Promising Eggplant Varieties Using RAPD Markers

1970 ◽  
Vol 19 (2) ◽  
pp. 119-126 ◽  
Author(s):  
Md. Sanaullah Biswas ◽  
Md. Abdullah Yousuf Akhond ◽  
Md. Al-Amin ◽  
Mahmuda Khatun ◽  
Muhammed Rezwan Kabir
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Genetic Relationship ◽  
Plant Tissue ◽  
Rapd Markers ◽  
Gene Diversity ◽  
Similarity Index ◽  
The Other ◽  
Upgma Dendrogram ◽  
Potential Tool

RAPD technique was used as a tool for assessing genetic diversity and varietal relationships among ten varieties of eggplant. Out of 21 primers screened four were selected. With these primers 76 clear and bright fragments were obtained of which 44 fragments considered polymorphic. The proportion of polymorphic loci and gene diversity values across all loci were 57.89% and 0.23, respectively. The UPGMA dendrogram based on genetic distance segregated the ten varieties of eggplant into two main clusters. Dohazari, Kazla, Nayantara and ISD-006 were grouped together in cluster I whereas Uttara, Islampuri, Khatkhatia, Singnath, BARI Begun-08 and Eggplant Line-083 into cluster II. Kazla and Nayantara variety pair was very close to each other with the highest intervarietal similarity index (92.54%) and lowest genetic distance (0.14). On the other hand, Khatkhatia and Nayantara pair was the lowest intervarietal similarity index (41.67%) with highest genetic distance (0.48). Therefore, identification of genetically distinct varieties using RAPD markers could be a potential tool for eggplant improvement. Key words: Eggplant, Polymorphism, Genetic relationship, RAPD D.O.I. 10.3329/ptcb.v19i2.5006 Plant Tissue Cult. & Biotech. 19(2): 119-126, 2009 (December)

Estimating genetic diversity in Greek durum wheat landraces with RAPD markers

2005 ◽  
Vol 56 (12) ◽  
pp. 1355 ◽  
Author(s):  
Anna Mantzavinou ◽  
Penelope J. Bebeli ◽  
Pantouses J. Kaltsikes
Keyword(s):  
Genetic Diversity ◽  
Durum Wheat ◽  
Bread Wheat ◽  
Rapd Markers ◽  
Triticum Turgidum ◽  
Random Amplified Polymorphic Dna ◽  
Triticum Aestivum L ◽  
The Other ◽  
Wheat Cultivars ◽  
Arbitrary Primers

Using the random amplified polymorphic DNA (RAPD) method, the genetic diversity of 19 Greek landraces and 9 cultivars of durum wheat [Triticum turgidum L. var. durum (Desf.)] was studied. Two commercial bread wheat (Triticum aestivum L.) cultivars and one genotype of Triticum monococcum L. were also included in the study. Eighty-seven arbitrary primers (10-mer) were evaluated in a preliminary experiment and 15 of them were selected for the main experiments based on the quality and reliability of their amplification and the polymorphism they revealed. A total of 150 DNA bands were obtained, 125 (83.3%) of which were polymorphic. On average, 10 DNA bands were amplified per primer, 8.3 of which were polymorphic. The genetic similarity between all pairs of genotypes was evaluated using the Jaccard’s or Nei and Li’s coefficients; the values of the former ranged from 0.153 to 0.973 while those of the latter were slightly higher (0.265–0.986). Cluster analysis was conducted by the UPGMA and the Njoin methods. Both methods broadly placed 26 durum genotypes into 1 branch while the other branch consisted of 2 subgroups: 1 included the 2 bread wheat cultivars; the other 1 consisted of 2 durum landraces, ‘Kontopouli’ and ‘Mavrotheri-Chios’, which showed an intruiging behaviour sharing bands with the bread wheat cultivars. The T. monococcum cultivar stood apart from all other genotypes.

Genetic diversity in Cinnamomum tamala Nees. accessions through DNA fingerprinting using molecular markers

2016 ◽  
Author(s):  
S Zafar Haider ◽  
Ujjwal . Bhandari ◽  
Gaurav . Naik ◽  
Nirpendra . Chauhan
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Rapd Markers ◽  
Issr Markers ◽  
Initial Screening ◽  
Cinnamomum Tamala ◽  
Upgma Cluster Analysis ◽  
Polymorphic Bands ◽  
Rapd And Issr ◽  
Uttarakhand Himalaya

The present study aims to investigate the genetic variation in Cinnamomum tamala Nees. (Lauraceae) accessions collected from different locations of Uttarakhand Himalaya. The leaves samples were analyzed by using RAPD and ISSR markers. A total 22 primers were used for initial screening in order to select the ones giving good amplification. Seven primers (OPA-12, OPA-18, OPB-10, OPB-17, ISSR-21, ISSR-24 and ISSR-30) were found to be polymorphic in eight accessions. Based on combined profile of ISSR and RAPD markers the dendrogram was constructed by using UPGMA cluster analysis and all the accessions showed discrimination from one another. All the accessions were clustered into two major groups, one containing T1-T4 and second T5-T8. The percentage of polymorphic bands was 86.3 % for three ISSR markers and 70.5 % for four RAPD markers. This emphasize that ISSR markers were found to be the best for genetic variation in the species.

scholarly journals Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers

2010 ◽  
Vol 10 (3) ◽  
pp. 204-210 ◽  
Author(s):  
Elizângela Almeida Rocha ◽  
Luciano Vilela Paiva ◽  
Humberto Henrique de Carvalho ◽  
Claudia Teixeira Guimarães
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Ssr Markers ◽  
Genomic Dna ◽  
Rapd Markers ◽  
Potato Cultivars ◽  
High Information Content ◽  
Commercial Potato ◽  
Polymorphic Bands ◽  
High Information

This study aimed to evaluate the genetic diversity and identify potato cultivars by RAPD and SSR markers. The genomic DNA of 16 potato cultivars was amplified with 25 RAPD primers that generated 92 polymorphic bands and 20 SSR primer pairs that produced 136 polymorphic bands. The dendrograms generated by cluster analysis distinguished the cultivars genetically although the dendrograms were not correlated in the comparison of the two markers used. The PIC values demonstrated the high information content of the primers used and 16 potato varieties were identified based on six RAPD primers and three SSR primer pairs. Thus, by means of RAPD and SSR markers the genetic diversity was assessed and the 16 commercial potato cultivars analyzed in this study were identified.

scholarly journals Genetic Diversity among Witchhazel Cultivars Based on Randomly Amplified Polymorphic DNA Markers

1997 ◽  
Vol 122 (4) ◽  
pp. 529-535 ◽  
Author(s):  
Robert D. Marquard ◽  
Eric P. Davis ◽  
Emily L. Stowe
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
North American ◽  
Dna Markers ◽  
Rapd Markers ◽  
North American Species ◽  
The Other ◽  
Randomly Amplified Polymorphic Dna ◽  
The North ◽  
Sib Families

Forty selections, including 37 cultivars of Hamamelis spp., were evaluated for genetic similarities using randomly amplified polymorphic DNA (RAPD) markers. Cluster analysis identified seven groups, which included three groups of H. ×intermedia cultivars, two groups of H. vernalis, and one group each of H. mollis and H. japonica. Three H. ×intermedia cultivars, `Arnold Promise', `Westerstede', and `Carmine Red', did not group closely with the other 20 cultivars of H. ×intermedia. Selections of the North American species H. vernalis were quite distinct from the Asiatic selections. However, data are presented that suggest hybridization exist between Asiatic Hamamelis spp. and H. vernalis. Genetic similarities between known half-sib families provides evidence that the cultivar pairs `Arnold Promise'—`Winter Beauty' and `Carmine Red'—`Hiltingbury' are, themselves, not likely half-sibs.

Sign in / Sign up

Export Citation Format

Share Document