scholarly journals Genetic diversity among Asparagus species using morphological characteristics and RAPD markers in Pakistan

2019 ◽  
Vol 5 (1) ◽  
pp. 13-24
Author(s):  
M Irshad ◽  
M Idrees ◽  
A Tariq ◽  
ML Pathak ◽  
M Hanif ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Field Experiments ◽  
Morphological Characteristics ◽  
Dry Weight ◽  
Phenotypic Variance ◽  
Neighbor Joining ◽  
Comparative Performance ◽  
Shoot Height

The aim of present research was to study the genetic diversity among Asparagus species and its cultivars using morphological characteristics and RAPD markers. In-vitro and field experiments of 14 germplasm sources of Asparagus species and its cultivars were conducted at the Kohat University of Science and Technology to estimate the comparative performance. Highest genotypic variance, phenotypic variance, genotypic and phenotypic coefficient of variance were observed in shoot height in shoot related traits were 318.40, 320.30, 939.34 and 944.96, respectively, whereas highest values for root related traits were observed in root length that were 21.84, 22.35, 141.60 and 144.91 respectively. Among the shoot related traits maximum heritability, genetic advance and genetic gain were calculated for shoot length, while in root related traits these characters were highest for root dry weight. RAPD markers were used to evaluate genetic diversity analysis of 14 germplasm sources of Asparagus species and its cultivars. RAPD markers generated a total of 247 bands, of these 239 bands were polymorphic with average of 34.1 bands per primer. Cluster analysis based on Neighbor Joining methods showed that wild species (A. adsendens, A. densiflorus, A. capitatus, A. gracelus, A. plumosus, A. racemosus and A. setaceus) were genetically distant from A. officinalis and its cultivars (Abril, Apollo, Gersengum, Huchel, Para seletion and Taranga). The results of the present investigations could be particularly used for authentic identification and would be useful for evaluation of genetic improvement of Asparagus species and its cultivars. J. Biodivers. Conserv. Bioresour. Manag. 2019, 5(1): 13-24

scholarly journals Two new species of Undifilum, fungal endophytes of Astragalus (locoweeds) in the United States

Botany ◽  
2012 ◽  
Vol 90 (9) ◽  
pp. 866-875 ◽  
Author(s):  
Deana L. Baucom ◽  
Marie Romero ◽  
Robert Belfon ◽  
Rebecca Creamer
Keyword(s):  
New Species ◽  
New Mexico ◽  
Random Amplified Polymorphic Dna ◽  
Phylogenetic Analyses ◽  
Fungal Endophytes ◽  
Morphological Characteristics ◽  
Its Region ◽  
The United States ◽  
Neighbor Joining

New species of Undifilum , from locoweeds Astragalus lentiginosus Vitman and Astragalus mollissimus Torr., are described using morphological characteristics and molecular phylogenetic analyses as Undifilum fulvum Baucom & Creamer sp. nov. and Undifilum cinereum Baucom & Creamer sp. nov. Fungi were isolated from dried plants of A. lentiginosus var. araneosus , diphysus , lentiginosus , and wahweapensis collected from Arizona, Oregon, and Utah, USA, and A. mollissimus var. biglovii , earleii , and mollissimus collected from New Mexico, Oklahoma, and Texas, USA. Endophytic fungi from Astragalus locoweeds were compared to Undifilum oxytropis isolates obtained from dried plant material of Oxytropis lamberteii from New Mexico and Oxytropis sericea from Arizona, Colorado, New Mexico, Utah, and Wyoming. Extremely slow growth in vitro was observed for all, and conidia, if present, were ellipsoid with transverse septa. However, in vitro color, growth on four different media, and conidium size differed between fungi from Astragalus spp. and U. oxytropis. Neighbor-joining analyses of internal transcribed spacer (ITS) region and glyceraldehyde-3-phosphate dehydrogenase (GPD) gene sequences revealed that U. fulvum and U. cinereum formed a clade distinct from U. oxytropis. This was supported by neighbor-joining analyses of results generated from random amplified polymorphic DNA (RAPD) fragments using two different primers.

scholarly journals Random Amplification of Polymorphic DNA Analysis for Genetic Characterization of Two Breeds of Dogs, German Shepherd and Japanese Spitz

2013 ◽  
Vol 13 (2) ◽  
pp. 73-78
Author(s):  
Jarina Joshsi ◽  
Lumanti Manandhar ◽  
Patima Shrestha ◽  
Rani Gupta ◽  
Rojlina Manadhar ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Dna Analysis ◽  
Genetic Characterization ◽  
Random Amplified Polymorphic Dna ◽  
Neighbor Joining ◽  
German Shepherd ◽  
Polymorphic Loci ◽  
Random Amplification

Random amplified polymorphic DNA (RAPD) markers were used to study genetic diversity in dog samples belonging to populations of German Shepherd and Japanese Spitz. A total of twelve samples were typed using eight RAPD primers. Out of eight primers, three primers gave result in six individuals of dogs. The phylogenetic tree constructed by the neighbor joining method based on Nei. Original measures revealed highest genetic identity found in German Shepherd as 0.9444 and highest genetic distance as 1.2809. The analysis predicts the number of polymorphic loci as 15 and the percentage of polymorphic loci as 83.3. Nepal Journal of Science and Technology Vol. 13, No. 2 (2012) 73-78 DOI: http://dx.doi.org/10.3126/njst.v13i2.7717

Digestibility and chemical composition of fractions of lucerne during spring and summer

1970 ◽  
Vol 75 (2) ◽  
pp. 213-222 ◽  
Author(s):  
K. R. Christian ◽  
D. B. Jones ◽  
M. Freer
Keyword(s):  
Chemical Composition ◽  
Dry Matter ◽  
Dry Weight ◽  
Chemical Components ◽  
Shoot Height ◽  
Time Of Year ◽  
Highly Correlated ◽  
Leaf Composition

SUMMARYBulk harvests of primary growth were made at intervals during the spring and summer of 2 consecutive years from an ungrazed area of lucerne (Medicago sativa) and the digestibility by sheep was measured. At the same times, shoots of known length representative of the sward were collected, divided into 7·5 cm lengths, and separated into leaf and stem for the determination of ash, nitrogen, cellulose, and in vitro organic matter digestibility.Digestibility and leaf: stem ratio of the bulk harvests decreased and dry matter increased as the season advanced. Leaf composition changed little, but stem increased in lignin and decreased in ash and in vitro digestibility. In vitro digestibility and leaf: stem ratio were highly correlated with sheep digestibility.Chemical composition of shoots of known heights was similar to that of the bulk harvests. Leaf weights/shoot tended to increase linearly with height of shoot, whereas stem increased nearly as the square of the height. Dry matter of leaf and stem were inversely related to leaf:stem ratio. Stem digestibility decreased with leaf:stem ratio and with increase in shoot height.Chemical components of shoot fractions were associated with each other and were largely dependent on the distance from the top of the shoot at which they had been taken, irrespective of shoot height or time of year. Bottom stems had lower ash, nitrogen and digestibility but higher cellulose, dry weight and dry matter than top stems, whose composition approached that of leaves. Leaf fractions showed little consistent trend with position on the shoot.Some of the difficulties in studying changes in plant composition under field conditions are discussed.

In vitro culture of Chrysanthemum plantlets using light-emitting diodes

2008 ◽  
Vol 3 (2) ◽  
pp. 161-167 ◽  
Author(s):  
Anželika Kurilčik ◽  
Renata Miklušytė-Čanova ◽  
Stasė Dapkūnienė ◽  
Silva Žilinskaitė ◽  
Genadij Kurilčik ◽  
...  
Keyword(s):  
Light Emitting Diodes ◽  
Spectral Component ◽  
Dry Weight ◽  
Chrysanthemum Morifolium ◽  
Photon Flux ◽  
Photon Flux Density ◽  
Shoot Height ◽  
Light Emitting ◽  
Blue Component

AbstractEffects of illumination spectrum on the morphogenesis of chrysanthemum plantlets (Chrysanthemum morifolium Ramat. ‘Ellen’) grown in vitro were studied using an illumination system consisting of four groups of light-emitting diodes (LEDs) in the following spectral regions: blue (450nm), red (640nm), red (660nm), and far-red (735nm). Taking into account all differences in shoot height, root length, and fresh and dry weight (FW and DW, respectively), observed while changing the total photon flux density (PFD), the optimal total PFD for growth of chrysanthemum plantlets in vitro was estimated. For 16 h photoperiod and typical fractions of the spectral components (14%, 50%, 28%, and 8%, respectively), the optimal total PFD was found to be 40 µmol m−2 s−1. Our study shows that the blue component in the illumination spectrum inhibits the plantlet extension and formation of roots and simultaneously increases the DW to FW ratio and content of photosynthetic pigments. We demonstrate photomorphogenetic effects in the blue region and its interaction with the fractional PFD of the far-red spectral component. Under constant fractional PFD of the blue component, the root number, length of roots and stems, and fresh weight of the plantlets have a correlated nonmonotonous dependence on the fractional PFD of the far-red component.

Genetic diversity in in vitro-conserved germplasm of Curcuma L. as revealed by RAPD markers

2008 ◽  
Vol 52 (4) ◽  
pp. 627-633 ◽  
Author(s):  
Z. Hussain ◽  
R. K. Tyagi ◽  
R. Sharma ◽  
A. Agrawal
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers

scholarly journals Influence of culture vessel volume and ventilation on in vitro culture and adenosine accumulation of Anoectochilus setaceus Blume

2018 ◽  
Vol 15 (2) ◽  
pp. 307-317
Author(s):  
Vũ Quốc Luận ◽  
Nguyễn Bá Nam ◽  
Vũ Thị Hiền ◽  
Nguyễn Phúc Huy ◽  
Hoàng Thanh Tùng ◽  
...  
Keyword(s):  
Liquid Medium ◽  
Dry Weight ◽  
Culture Conditions ◽  
Culture Vessel ◽  
Shoot Height ◽  
Factors Affecting ◽  
Vessel Volume ◽  
Plastic Bag ◽  
Tlc Analysis

Anoectochilus setaceus is a medicinal plant used as a traditional medicine as well as nutritious products for health care in China and many Asian countries. In conventional propagation, the the vessel type, material and volume lead to differences in the moisture content between the internal and external conditions which result in great impact on the quality of in vitro plantlets as well as subsequent growth at nursery. In this study, several factors affecting the multiplication and regeneration of A. setaceus cultured in vitro were investigated. The highest percentage of shoot regeneration (52.88%) from ex vitro stem nodes was obtained when explants were cultured on SH medium supplemented with 1 mg/l BA, 30 g/l sucrose with cotton wool plug as the substrate and pH of the medium was adjusted to 5.8 prior to autoclaving. In the present study, culture vessel volume and ventilation culture conditions indicated to have effect on shoot quality. Results showed that 3-liter Erlenmeyer flasks in combination with ventilation caps resulted in better shoot growth and development (shoot height: 10.75 cm; 9.45 leaves per shoot; fresh- and dry- weight of 2.45 and 0.219 g per shoot, respectively; leaf diameter: 2.85 cm and SPAD: 39.99). Results of chromatography (TLC) analysis indicated that explants gave the same Rf value as that of authentic Adenosine. Qualifying Adenosine using TLC showed that extracts of shoots cultured on solid and liquid medium (ventilation plastic bag), and that of shoots cultured in 3-liter vessel with liquid medium (with and without ventilation) gave the same pattern in the respects of color and Rf value.

scholarly journals (447) Response of Two Salt-stressed Cultivars of Sweetpotato to Changes in Calcium Levels under In Vitro Conditions

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1035A-1035
Author(s):  
Devi Prasad V. Potluri ◽  
Nkechiyere Nwami
Keyword(s):  
Salt Stress ◽  
Dry Weight ◽  
Proline Accumulation ◽  
Metabolic Changes ◽  
Soluble Carbohydrate ◽  
Ms Medium ◽  
Shoot Height ◽  
Weight Number ◽  
Adverse Affects

Two cultivars of sweetpotato, `Commensal' and `Salyboro', were subjected to salt stress using axillary bud cultures. The salt levels ranged from 0 to 10 g·L-1. After the initial experiments, levels of calcium in the medium were changed from 3 mm in the MS medium to 1.5, 6, and 12 mm. After 10 weeks of growth, plantlet shoot height, dry weight, number of nodes, levels of proline, soluble carbohydrate, and protein were measured. `Commensal' was tolerant to salt levels up to 4 g·L-1, but `Salyboro' was sensitive to concentrations of salt even at lower concentrations as evidenced by the growth and dry weight. Proline accumulation was higher in the shoot than in the root. The protein: carbohydrate ratios did not change much in `Commensal', but levels of carbohydrates increased in `Salyboro'. Reduction in calcium levels had a synergistic affect on salt-stressed cultivars. Enhanced levels of calcium reduced the inhibitory affects of salt stress. This was more pronounced in `Salyboro', which was susceptible. Proline levels were higher in plants subjected to salt stress and higher levels of calcium than controls, but lower than the plants subjected to salt stress. These and other metabolic changes suggest that calcium can reduce the adverse affects of salt stress in these two sweetpotato cultivars.

scholarly journals (295) Relationship Between Macromolecule and Metal Ion Accumulation in Two Salt-stressed Cultivars of Sweetpotato under In Vitro Conditions

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 1079A-1079
Author(s):  
Devi Prasad V. Potluri
Keyword(s):  
Salt Stress ◽  
Metal Ion ◽  
Ipomoea Batatas ◽  
Dry Weight ◽  
Soluble Carbohydrates ◽  
Soluble Carbohydrate ◽  
Shoot Height ◽  
Sodium Accumulation ◽  
Carbohydrate Levels

Two cultivars of sweetpotato [Ipomoea batatas (L.) Lam.], Commensal and Salyboro, were subjected to salt stress using axillary bud cultures. The salt levels ranged from 0–150 mM. After 10 weeks of growth, plantlet shoot height, dry weight, number of nodes, levels of proline, soluble carbohydrate, and protein; and metal ions sodium, potassium, magnesium, and calcium, were measured. In both cultivars, proline accumulation was higher in the shoot. There was a positive correlation between the increase in soluble carbohydrates and proteins in `Commnesal', but not in `Salyboro'. More sodium accumulated in the shoots of `Salyboro' compared to `Commensal'. The accumulation of sodium reduced the calcium and potassium, but not magnesium levels. Increase in sodium levels correlated with the increase in soluble carbohydrate levels is `Salyboro', but not in `Commensal'. A similar trend was evident with praline and sodium accumulation. Based on these and previous results, the cultivar `Salyboro' appears to be more susceptible to salt stress.

Morphological variation among hemp dogbane (Apocynum cannabinum) populations

Weed Science ◽  
1998 ◽  
Vol 46 (1) ◽  
pp. 71-75 ◽  
Author(s):  
Corey V. Ransom ◽  
James J. Kells ◽  
Loyd M. Wax ◽  
Michael S. Orfanedes
Keyword(s):  
Morphological Variation ◽  
Leaf Shape ◽  
Morphological Characteristics ◽  
Dry Weight ◽  
Degree Days ◽  
Shoot Height ◽  
Shoot Dry Weight ◽  
Shoot Number ◽  
Emergence Date

Experiments were conducted to examine morphological variation among hemp dogbane populations. Rootstocks collected from 16 sites throughout Michigan and Illinois were used to establish nurseries in East Lansing, MI, and Champaign, IL. Growth, stem characteristics, and leaf characteristics were measured for each ecotype at both nurseries. Differences among ecotypes were observed for all measurements except emergence date and growing degree days to emergence. The number of shoots per plot produced by the ecotypes ranged from 5 to 54 and shoot height ranged from 69 to 126 cm. Ecotypes spread laterally at different rates, with the most aggressive covering 19 times more ground area than the least aggressive. Total shoot dry weight accumulation varied greatly among ecotypes. Some ecotypes could be identified from others by their unique leaf shape. Shoot number, shoot height, and ground area covered were greater for plants grown in Michigan than in Illinois. Differences in growth and morphological characteristics among ecotypes were not correlated with the geographical region where they were collected. Differences in measurements between the two nurseries illustrate the role of environment and genetics in the growth and morphology of this plant species.

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