Study of influence of biopreparate «Microfit» for the condition internal organs of laboratory rats

2017 ◽  
pp. 37-40
Author(s):  
K.D. Zakarya ◽  
◽  
Z.S. Sarmurzina ◽  
R.T. Dospaeva ◽  
G.N. Bissenova ◽  
...  
Keyword(s):  
Internal Organs ◽  
Laboratory Rats

scholarly journals Histological study of the drug PEG-Filstim sub-acute toxicity

2018 ◽  
pp. 80-88
Author(s):  
V. L. Karbovskyy ◽  
I. A. Shevchuk ◽  
O. V. Kurkina ◽  
T. Ye. Makovska
Keyword(s):  
Defect Formation ◽  
Histological Study ◽  
Subcutaneous Administration ◽  
Toxic Effects ◽  
Internal Organs ◽  
Brain Vessels ◽  
Laboratory Rats ◽  
Applied Dose ◽  
Reticular Tissue ◽  
The Brain

One of the critical steps in development of safe and efficient drugs during their pre-clinical trials are toxicity studies. Therefore, the aim of our work was to study PEG-Filstim toxic effects on animal internal organs and tissues. Toxicity study of PEG-Filstim was performed in 50 white wild-type rats of both sexes with body weight of 170 to 230 g on daily (28 days) subcutaneous administration in the doses of 0.5, 1.0 and 2.0 mg/kg. In all groups of animals, after completing the experiment careful pathomorphologic and histological examination was performed. PEG-Filstim has been shown to possess no toxic effects on internal organs of laboratory rats and does not cause specific changes in the heart, kidneys and mucous coat of stomach on daily subcutaneous administration in the doses of 0.5, 1.0, and 2.0 mg/kg within 28 days. In the maximum applied dose of 2.0 mg/kg, the studied drug causes pronounced acute splenic hyperplasia, related to hyper-proliferation of the reticular tissue, leads to functional strain of the liver due to formation of hematopoietic foci in it, as well as impaired integrity of the respiratory epithelium and congestive signs in the lungs, swelling of the brain tissues, abnormalities in the gray matter structure and hyperemia of the brain vessels. These effects were not observed in the animals, to which the drug was administered in the doses of 0.5 and 1.0 mg/kg. Administration of PEG-Filstim (in all studied doses) results in increasing the size of the ankle joint in rats, which is related to hyper-proliferation of the reticular tissue, leading to bone defect formation in the form of perforation with subsequent filling the periosteum with reticular tissue and formation of hematopoietic foci within its boundaries.

scholarly journals Histostructure of the internal organs and skin of rats for the long-term effect of the drug “VetMikoDerm”

2019 ◽  
Vol 21 (94) ◽  
pp. 136-141
Author(s):  
V. P. Martynyshyn
Keyword(s):  
Skin Toxicity ◽  
Total Surface Area ◽  
Control Group ◽  
Histological Structure ◽  
Renal Tubules ◽  
Internal Organs ◽  
Laboratory Rats ◽  
Long Term Effect ◽  
Long Time ◽  
Experimental Group

The investigation of the skin toxicity of the liniment “VetMikoDerm” for prolonged use was performed on young and healthy laboratory rats with intact skin, body weight 200–220 g. The investigated environment was applied to a clean, non-woolly area (dorsal/lateral surface) of at least 10% of the total surface area. The spotted thistle oil was applied to the pre-prepared area of the skin of the animals of the control group, and the other two experimental groups of animals got the studied drug at the doses of 50 (I) and 500 mg/kg (II), respectively. The drug was applied to the skin daily for 28 days. At the end of the experiment, after the decapitation of the rats conducted under the light etheric anesthesia, a complete pathoanatomical dissection was performed, the coefficients of the mass of the internal organs were determined, samples of liver, kidney and skin tissues were taken for their histological examination. According to the results it was established that the liver and kidneys histology, and skin of rats of the 1st experimental group were similar to those that were of the control animals. At the same time, receiving the maximum dose (10 times the therapeutic dose) of the drug “VetMikoDerm”, the majority of rats of the 2nd group showed a discomplication of the lamellar structure of the liver lobules, hepatocytes were placed in separate groups, sinusoidal capillaries were expanded. The presence of hepatocytes with heterogeneous, granular and weakly colored cytoplasm was observed in the central parts of the lobules, the nuclei of individual hepatocytes were increased, indicating the development of granular protein degeneration of the parenchyma. Histologically, under these conditions, foci of granular dystrophy of the epithelium of the vorticular and direct renal tubules with the expansion of their lumen were found in the structure of kidney of rats. The kernels of individual nephropyelitis had signs of karyopicnosis and cariorexis. The histological structure of skin of rats in all experimental groups was of the same type and consisted of the epidermis, dermis and hypodermis. Animals of the 2nd experimental group which repeatedly and for a long time were applied a “VetMikoDerm” lineulation in a dose that exceeded its optimal amount by 10 times showed the epidermal thickening, the presence of small-mesenchymal polymorphocytic infiltration, microcirculatory corneal disorder and sebaceous gland hyperplasia  glands in the dermis that indicated the development of a compensatory-adaptive reaction in places of application of a 10-fold dose of the investigational medicinal product.

scholarly journals HOP-PLANT CARBONATE EXTRACT. TOXICITY. ORGANIC CHANGES

2020 ◽  
Author(s):  
Tetiana Moiseienko ◽  
◽  
Gennadiy Khrystian ◽  
Inna Torianyk ◽  
◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Study Groups ◽  
The Body ◽  
Sebaceous Glands ◽  
Negative Effects ◽  
Internal Organs ◽  
Inflammatory Reactions ◽  
Laboratory Rats ◽  
Icd 10 ◽  
Severity Of The Disease

Acne is a leading player in the spread of skin diseases. Epidemiological studies conducted in industrialised Western countries have estimated the prevalence of acne in adolescents at between 50% and 95%, depending on the method used to calculate the lesions. Acne, a disease that most often befalls teenage faces, occurs in children after the onset of adrenal and gland androgens production and subsides after growth. However, it may continue to manifest itself in a large proportion of adults, especially women. Even after recovery, negative effects such as scars and pigment spots remain. Acne (L.70, eels, ICD-10) is a chronic polymorphic multifactorial inflammatory disease of the sebaceous glands and periglandular tissue that develops mainly in young people. To date, the causes of etiopathogenesis of BX have not been sufficiently investigated. According to ICD-10, the following factors are important in the development of the disease: 1) increase in skin fat production 2) excessive follicular hyperkeratosis; 3) microflora (Propionibacterium acnes) influence 4) inflammation development. Patients suffering from acne have significantly increased skin fat production, which is usually correlated with the severity of the disease. Changes in the physiological state of the seborrhea glands, which is the basis of seborrhea, develop under the influence of neuroendocrine system dysfunction. Hyperplasia and sebaceous gland hyper-secreting occur, the physical and chemical composition of seborrhea changes and its bactericidal properties are reduced. The imbalance of the autonomic nervous system is important, which leads to a temporary or permanent increase in the tone of the vagus innervation of the sebaceous glands and, as a result, hyperproduction of sebum. A number of researchers consider follicular hyperkeratosis to be one of the leading links in the pathogenesis of acne. The aim of the study was to study the chronic toxicity of a gel with an extract of carbon dioxide hops and to determine the effect of the latter on organ changes in laboratory animals. The methodological basis was microbiological, cultural, morphological and biochemical research methods. The duration of observation was 30 days. Both experimental prototypes of gel preparations and placebo were studied in the study groups. The results in the experiments were compared with intact controls. In an animal experiment (laboratory rats), it was found that gels with carbon dioxide hop extract do not exhibit toxic (chronic) effects on the body. Dynamics of the body mass of the individuals studied, blood parameters, including the constituent elements of the blood serum, CNS functioning parameters, structural and functional state of the urine and kidney systems are the objective evidence base for the use of the medicinal substance after the clinical stage of the studies. Based on the results of a pathomorphological study of the internal organs of experimental animals, it was established that the acne gel for long-term use in the studied dose does not affect the relative mass of the internal organs of rats and does not cause morphological and functional changes in them, does not lead to inflammatory reactions, destructive and degenerative processes and necrosis. Thus, the results of this study provide the basis for the treatment of acne with mudflow medications through their prolonged use. It has been determined that the gel does not have toxic effects on the skin and does not lead to negative effects on the internal organs of animals.

Toxicological characteristics of a medicinal product for veterinary «Ketoprofen 10%» in laboratory rats (subchronic toxicity)

2021 ◽  
Vol 1 (6) ◽  
pp. 34-41
Author(s):  
A. N. Shkatova ◽  
◽  
D. A. Devrishov ◽  
O. B. Litvinov ◽  
V. E. Brylina ◽  
...  
Keyword(s):  
Body Weight ◽  
Medicinal Product ◽  
Intramuscular Injection ◽  
Study Drug ◽  
The Body ◽  
Subchronic Toxicity ◽  
Internal Organs ◽  
Experimental Animals ◽  
Laboratory Rats

In a subchronic experiment with intramuscular injection in rats, it was found that a dose of 70,7 mg/kg is toxic, and doses of 35,4 and 17,2 mg/kg are inactive (safe). It was noted that the 14 day administration of the drug «Ketoprofen 10%» in a dose 70,7 mg/kg caused a decrease in the body weight of experimental animals, contributed to the development of hepatotoxic action, a change in the mass coefficients of internal organs. The above effects were reversible 10 days after discontinuation of the study drug.

scholarly journals Research of acute toxicity of the drug HEXIA

2018 ◽  
pp. 88-94
Author(s):  
V. L. Karbovskyy ◽  
I. A. Shevchuk ◽  
O. V. Kurkina ◽  
T. Ye. Makovska
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Daily Basis ◽  
Relative Mass ◽  
Intragastric Administration ◽  
Internal Organs ◽  
Laboratory Rats ◽  
Rats And Mice ◽  
The Impact ◽  
Female Laboratory

Diseases of the genitourinary system caused by pathogenic and potentially pathogenic microorganisms, which result into disbiosis of urinary organs, remain an urgent problem of dermatovenereology, gynecology and urology, despite the fact that there is a significant number of available and new medicines to treat them. The aim of the work was to determine the safety of the preparation Hexia within experiments on animals. The acute toxicity of Hexia has been determined on 20 adult female laboratory rats under the conditions of hourly intravaginal administration of the preparation with a dose of 70 mg/kg during 12 hours, as well as on 20 female laboratory rats and 20 female laboratory mice with a single intragastric administration in a dose of 145 mg/kg. The assessment of the impact of the preparation studied was performed on the basis of the following parameters: a) mortality (terms of death of animals in each group, on a daily basis); b) assessment of toxicity development (on a daily basis), including an assessment of the visual environment of the area of injection (the presence of irritation, redness, edema); c) dynamics of body weight changes (in the initial state, on the 4th, 7th and 14th day after application); d) macroscopy of internal organs, mass coefficients of internal organs within rats (on the 14th day). It has been found that intravaginal application and a single intragastric administration of the preparation Hexia in the form of pessaries, which contain chlorhexidine digluconate, does not result into death of rats and mice, brings no effect on body weight gain, integrative parameters of the functional state of laboratory animals as well as on the relative mass of internal organs, which implies the absence of significant toxic effect of the preparation. Thus, the results of the studies conducted indicate that the median lethal dose for Hexia in case of intravaginal application to rats or intragastric administration to rats and mice is beyond the rate of 500 mg/kg. According to the toxicological classification of substances Hexia belongs to the IV class of toxicity – low toxic substances.

Alternative pathways in Angiostrongylus cantonensis (Metastrongyloidea: Angiostrongylidae) transmission

Parasitology ◽  
2020 ◽  
pp. 1-7
Author(s):  
David Modrý ◽  
Barbora Fecková ◽  
Barbora Putnová ◽  
Sheina Macy Manalo ◽  
Domenico Otranto
Keyword(s):  
Rattus Norvegicus ◽  
Angiostrongylus Cantonensis ◽  
Experimental Exposure ◽  
Internal Organs ◽  
Gastropod Species ◽  
Alternative Pathways ◽  
Laboratory Rats ◽  
Third Stage ◽  
Infection Pathways ◽  
Human Infections

Abstract In order to elucidate the infection pathways of third stage larvae (L3) of Angiostrongylus cantonensis, we performed experiments to assess: (i) the shedding of L3 from two species of experimental veronicellid slugs drowned in water and the ratio of emerged larvae, (ii) the transmission of viable L3 from drowned terrestrial gastropods to aquatic snails, and (iii) the transmission of viable L3 between terrestrial snails. Molluscs were experimentally infected by first stage larvae (L1) of A. cantonensis. Significantly more L3 larvae were released from Veronicella cubensis than from Veronicella sloanei. Numerous L3 were observed in the muscular foot, and also in the connective tissue between internal organs. Experimental exposure of P. maculata to L3 of A. cantonensis liberated from other gastropod species led to their infection and the infectivity of larvae after intermediesis was demonstrated by infection of laboratory rats (Rattus norvegicus). The transmission of L3 was observed in three out of four experiment replications and L3 were retrieved from 6 out of 24 Subulina octona snails. The infected synanthropic molluscs represent a key component in the epidemiology of human infections by A. cantonensis. Escape of L3 larvae from bodies of dead snails or slugs and their ability to infect further gastropod hosts (intermediesis) represents a public health risk. Thus, control of molluscs living in peri-domestic environment is an essential part of prevention of human infections.

Context Aversion, Pavlovian Conditioning, and the Psychological Side Effects of Chemotherapy

1997 ◽  
Vol 2 (2) ◽  
pp. 118-124
Author(s):  
Geoffrey Hall
Keyword(s):  
Animal Model ◽  
Side Effects ◽  
Theoretical Analysis ◽  
Conditioned Response ◽  
Behavioral Strategies ◽  
Contextual Cues ◽  
Anticipatory Nausea ◽  
Laboratory Rats ◽  
Anticipatory Nausea And Vomiting ◽  
Parallel Case

Patients who have undergone several sessions of chemotherapy for cancer will sometimes develop anticipatory nausea and vomiting (ANV), these unpleasant side effects occurring as the patients return to the clinic for a further session of treatment. Pavlov's analysis of learning allows that previously neutral cues, such as those that characterize a given place or context, can become associated with events that occur in that context. ANV could thus constitute an example of a conditioned response elicited by the contextual cues of the clinic. In order to investigate this proposal we have begun an experimental analysis of a parallel case in which laboratory rats are given a nausea-inducing treatment in a novel context. We have developed a robust procedure for assessing the acquisition of context aversion in rats given such training, a procedure that shows promise as a possible animal model of ANV. Theoretical analysis of the conditioning processes involved in the formation of context aversions in animals suggests possible behavioral strategies that might be used in the alleviation of ANV, and we report a preliminary experimental test of one of these.

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