Videogrammetric Methods in Velocimetry

1998 ◽  
Vol 51 (6) ◽  
pp. 387-413 ◽  
Author(s):  
Th. Dracos ◽  
A. Gruen
Keyword(s):  
High Speed ◽  
Imaging Techniques ◽  
Three Dimensional ◽  
Light Sheet ◽  
Digital Data ◽  
Simultaneous Imaging ◽  
Observation Volume ◽  
Stage Of Development ◽  
Long Time ◽  
Lagrangian Measurements

Photogrammetry has been successfully applied in surveying and mapping for a long time. New imaging techniques, digital data acquisition and storage and powerful computing facilities transformed photogrammetry into videogrammetry, a technique applied in many fields for precise and reliable position measurements. Two applications of videogrammetry in fluid mechanics are presented in this article. Both are at their present stage of development suited for three-dimensional velocity measurements in liquids. One is based on tracking particles seeded in the liquid by using three to four synchronized video-cameras (PTV). It allows one to determine accurately the velocity vectors at a large number of points inside a thick observation volume and also to follow the trajectories of these particles for sufficiently long time periods. It is especially well suited for Lagrangian measurements in flows. The other tracks small three-dimensional patterns in flows of liquids tagged by fluorescent dye (LIFV). The three-dimensional Laser Induced Fluorescence images needed are obtained by sweeping rapidly a thin laser-light sheet with simultaneous imaging using a high speed solid-state camera. The method yields the shift and deformations of the liquid volumes associated with these patterns and allows one to determine velocity vectors and their derivatives simultaneously and accurately at a large number of points inside the observation volume. This review article includes 100 references.

scholarly journals SPATIO-TEMPORAL DATA ANALYSIS WITH NON-LINEAR FILTERS: BRAIN MAPPING WITH fMRI DATA

2011 ◽  
Vol 19 (3) ◽  
pp. 189
Author(s):  
Karsten Rodenacker ◽  
Klaus Hahn ◽  
Gerhard Winkler ◽  
Dorothea P Auer
Keyword(s):  
Signal To Noise Ratio ◽  
Three Dimensional ◽  
Digital Data ◽  
Linear Filters ◽  
Brain Functions ◽  
Non Linear ◽  
Long Time ◽  
Spatio Temporal ◽  
Sensory Activation ◽  
Temporal Data Analysis

Spatio-temporal digital data from fMRI (functional Magnetic Resonance Imaging) are used to analyse and to model brain activation. To map brain functions, a well-defined sensory activation is offered to a test person and the hemodynamic response to neuronal activity is studied. This so-called BOLD effect in fMRI is typically small and characterised by a very low signal to noise ratio. Hence the activation is repeated and the three dimensional signal (multi-slice 2D) is gathered during relatively long time ranges (3-5 min). From the noisy and distorted spatio-temporal signal the expected response has to be filtered out. Presented methods of spatio-temporal signal processing base on non-linear concepts of data reconstruction and filters of mathematical morphology (e.g. alternating sequential morphological filters). Filters applied are compared by classifications of activations.

scholarly journals Fast Objective Coupled Planar Illumination Microscopy

2018 ◽  
Author(s):  
Cody Greer ◽  
Timothy E. Holy
Keyword(s):  
Fluorescence Microscopy ◽  
High Speed ◽  
Imaging Techniques ◽  
Light Sheet ◽  
Frame Rate ◽  
Three Dimensions ◽  
Two Photon Microscopy ◽  
Image Sharing ◽  
Scanning Rate ◽  
Fast Light

Among optical imaging techniques light sheet fluorescence microscopy stands out as one of the most attractive for capturing high-speed biological dynamics unfolding in three dimensions. The technique is potentially millions of times faster than point-scanning techniques such as two-photon microscopy. However current-generation light sheet microscopes are limited by volume scanning rate and/or camera frame rate. We present speed-optimized Objective Coupled Planar Illumination (OCPI) microscopy, a fast light sheet technique that avoids compromising image quality or photon efficiency. We increase volume scanning rate to 40 Hz for volumes up to 700 µm thick and introduce Multi-Camera Image Sharing (MCIS), a technique to scale imaging rate by parallelizing acquisition across cameras. Finally, we demonstrate fast calcium imaging of the larval zebrafish brain and find a heartbeat-induced artifact that can be removed by filtering when the imaging rate exceeds 15 Hz. These advances extend the reach of fluorescence microscopy for monitoring fast processes in large volumes.

scholarly journals Wake Region Measurements of a Highly Three-Dimensional Nozzle Guide Vane Tested at DRA Pyestock Using Particle Image Velocimetry

1994 ◽  
Author(s):  
M. Funes-Gallanzi ◽  
P. J. Bryanston-Cross ◽  
K. S. Chana
Keyword(s):  
Flow Field ◽  
High Speed ◽  
Guide Vane ◽  
Three Dimensional ◽  
Light Sheet ◽  
Test Facility ◽  
Velocity Mapping ◽  
Nozzle Guide Vane ◽  
Nozzle Guide ◽  
Annular Cascade

The quantitative whole field flow visualization technique of PIV has over the last few years been successfully demonstrated for transonic flow applications. A series of such measurements has been made at DRA Pyestock. Several of the development stages critical to a full engine application of the work have now been achieved using the Isentropic Light Piston Cascade (ILPC) test facility operating with high inlet turbulence levels: • A method of seeding the flow with 0.5μm diameter styrene particles has provided an even coverage of the flow field. • A method of projecting a 1 mm thick high power Nd/YAG laser light sheet within the turbine stator cascade. This has enabled a complete instantaneous intra-blade velocity mapping of the flow field to be visualized, by a specially developed diffraction-limited optics arrangement. • Software has been developed to automatically analyze the data. Due to the sparse nature of the data obtained, a spatial approach to the extraction of the velocity vector data was employed. • Finally, a comparison of the experimental results with those obtained from a three-dimensional viscous flow program of Dawes; using the Baldwin-Lomax model for eddy viscosity and assuming fully turbulent flow. The measurements provide an instantaneous quantitative whole field visualization of a high-speed unsteady region of flow in a highly three-dimensional nozzle guide vane; which has been successfully compared with a full viscous calculation. This work represents the first such measurements to be made in a full-size transonic annular cascade at engine representative conditions.

Simple mechanisms organise orientation of escape swimming in embryos and hatchling tadpoles of Xenopus laevis

2000 ◽  
Vol 203 (12) ◽  
pp. 1869-1885 ◽  
Author(s):  
A. Roberts ◽  
N.A. Hill ◽  
R. Hicks
Keyword(s):  
Mathematical Model ◽  
Xenopus Laevis ◽  
High Speed ◽  
Three Dimensional ◽  
Longitudinal Axis ◽  
The Body ◽  
Video Recordings ◽  
Stage Of Development ◽  
Physical Features ◽  
Neutrally Buoyant

Many amphibian tadpoles hatch and swim before their inner ears and sense of spatial orientation differentiate. We describe upward and downward swimming responses in hatchling Xenopus laevis tadpoles from stages 32 to 37/38 in which the body rotates about its longitudinal axis. Tadpoles are heavier than water and, if touched while lying on the substratum, they reliably swim upwards, often in a tight spiral. This response has been observed using stroboscopic photography and high-speed video recordings. The sense of the spiral is not fixed for individual tadpoles. In ‘more horizontal swimming’ (i.e. in directions within +/−30 degrees of the horizontal), the tadpoles usually swim belly-down, but this position is not a prerequisite for subsequent upward spiral swimming. Newly hatched tadpoles spend 99 % of their time hanging tail-down from mucus secreted by a cement gland on the head. When suspended in mid-water by a mucus strand, tadpoles from stage 31 to 37/38 tend to swim spirally down when touched on the head and up when touched on the tail. The three-dimensional swimming paths of stage 33/34 tadpoles were plotted using simultaneous video images recorded from the side and from above. Tadpoles spiralled for 70 % of the swimming time, and the probability of spiralling increased to 1 as swim path angles became more vertical. Tadpoles were neutrally buoyant in Percoll/water mixtures at 1.05 g cm(−)(3), in which anaesthetised tadpoles floated belly-down and head-up at 30 degrees. In water, their centre of mass was ventral to the muscles in the yolk mass. A simple mathematical model suggests that the orientation of tadpoles during swimming is governed by the action of two torques, one of which raises the head (i.e. increases the pitch) and the other rotates (rolls) the body. Consequently, tadpoles (i) swim belly-down when the body is approximately horizontal because the body is ballasted by dense yolk, and (ii) swim spirally at more vertical orientations when the ballasting no longer stabilises orientation. Measurements in tethered tadpoles show that dorsal body flexion, which could produce a dorsal pitch torque, is present during swimming and increases with tailbeat frequency. We discuss how much of the tadpole's behaviour can be explained by our mathematical model and suggest that, at this stage of development, oriented swimming responses may depend on simple touch reflexes, the organisation of the muscles and physical features of the body, rather than on vestibular reflexes.

Electrically tunable lenses – eliminating mechanical axial movements during high-speed 3D live imaging

2021 ◽  
Vol 134 (16) ◽  
Author(s):  
Christoforos Efstathiou ◽  
Viji M. Draviam
Keyword(s):  
Cell Biology ◽  
High Speed ◽  
Imaging Techniques ◽  
Three Dimensional ◽  
Cost Effective ◽  
High Speed Imaging ◽  
Liquid Lenses ◽  
Live Cell Microscopy ◽  
Tunable Lenses ◽  
Cell Microscopy

ABSTRACT The successful investigation of photosensitive and dynamic biological events, such as those in a proliferating tissue or a dividing cell, requires non-intervening high-speed imaging techniques. Electrically tunable lenses (ETLs) are liquid lenses possessing shape-changing capabilities that enable rapid axial shifts of the focal plane, in turn achieving acquisition speeds within the millisecond regime. These human-eye-inspired liquid lenses can enable fast focusing and have been applied in a variety of cell biology studies. Here, we review the history, opportunities and challenges underpinning the use of cost-effective high-speed ETLs. Although other, more expensive solutions for three-dimensional imaging in the millisecond regime are available, ETLs continue to be a powerful, yet inexpensive, contender for live-cell microscopy.

scholarly journals Fast objective coupled planar illumination microscopy

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Cody J. Greer ◽  
Timothy E. Holy
Keyword(s):  
Fluorescence Microscopy ◽  
High Speed ◽  
Imaging Techniques ◽  
Light Sheet ◽  
Three Dimensions ◽  
Planar Imaging ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Larval Zebrafish ◽  
Fast Light

Abstract Among optical imaging techniques light sheet fluorescence microscopy is one of the most attractive for capturing high-speed biological dynamics unfolding in three dimensions. The technique is potentially millions of times faster than point-scanning techniques such as two-photon microscopy. However light sheet microscopes are limited by volume scanning rate and/or camera speed. We present speed-optimized Objective Coupled Planar Illumination (OCPI) microscopy, a fast light sheet technique that avoids compromising image quality or photon efficiency. Our fast scan system supports 40 Hz imaging of 700 μm-thick volumes if camera speed is sufficient. We also address the camera speed limitation by introducing Distributed Planar Imaging (DPI), a scaleable technique that parallelizes image acquisition across cameras. Finally, we demonstrate fast calcium imaging of the larval zebrafish brain and find a heartbeat-induced artifact, removable when the imaging rate exceeds 15 Hz. These advances extend the reach of fluorescence microscopy for monitoring fast processes in large volumes.

The Use of Virtuality in Car Development

2000 ◽  
Vol 44 (38) ◽  
pp. 828-831
Author(s):  
Ernst Assinann ◽  
Human Ramezani
Keyword(s):  
Laser Scanning ◽  
Early Stage ◽  
Three Dimensional ◽  
Car Industry ◽  
Stage Of Development ◽  
Wide Range ◽  
Long Time ◽  
Development Processes ◽  
Virtual Experiences ◽  
Application Fields

For many years in car development the future customer was first represented by templates. Today and in fact for a long time now CAD tools are solely used for designing a car and therefore man had to be integrated into that environment as well. 1986 the German car industry joined in a research program to produce a common man model for the use in automotive design. This program called RAMSIS has been in practical use at BMW since the early nineties. All “static” situations can be assessed with RAMSIS today including dynamic movements of arms and legs. For entry and egress and for the final confirmation, three dimensional mock-ups are tested by a number of in-house test subjects. Their body dimensions have to be known in order to compare their assessments with the customer population. Therefore we regularly measure members of the research and development center using all methods, from the conventional yardstick to current laser scanning techniques. Hand in hand with DMU methods Virtual Reality has gained access to development processes. The goal of DMU, to eliminate the time consuming and expensive hardware loops and replace them as much as possible by digital models, is effectively supported by VR techniques that speed up processes by enhancing the man-machine-interaction. There is a wide range of application fields to use these techniques, e.g. design review, assembly and maintenance simulation and training. RAMSIS is integrated in the BMW VR environment with special extensions to allow immersive ergonomic research. Mixed mock-up applications are used to have virtual experiences, to verify or to train assembly procedures at an early stage of development and therefore eliminate problems as soon as possible. New concepts can be evaluated and assessed taking ergonomic aspects and disturbing influences into account.

scholarly journals High speed rotary system for catheter based 3-D imaging with optical coherence tomography (OCT)

2021 ◽  
Author(s):  
Antonio Mauro
Keyword(s):  
Optical Coherence Tomography ◽  
High Speed ◽  
Imaging Techniques ◽  
Three Dimensional ◽  
Biological Tissues ◽  
Optical Coherence ◽  
X Rays ◽  
Sound Waves ◽  
Acquisition Rate

Optical Coherence Tomography (OCT) is an addition to the other tomographic imaging techniques of x-ray computed tomography, magnetic resonance imaging, and ultrasound imaging. OCT uses optical reflections of biological tissues as opposed to x-rays, RF fields, and sound waves to obtain images. A rotary and pullback system has been developed for use with OCT. The system was developed to facilitate the three dimensional imaging of various lumens in humans and animals. The system is capable of rotating at a rate of 200 Hz. At this rate the rotary system will allow for a frame acquisition rate of 200 fps which is significantly higher than the highest published acquisition rate to date of 108 fps. The probes used with the system were modeled after the Intravascular Ultrasound (IVUS) miniature torque cable design. The probes can be sealed and sterilized between subjects without being damaged; unlike the single use IVUS probes. The rotary system was used to image the outer ear of a mouse in vivo. A lateral slice from the resulting three dimensional image was compared to the general histology of a mouse ear. The image compared well to the general anatomy as found on the histology.

Three-Dimensional Particle Tracking Velocimetry With Laser-Light Sheet Scannings

1996 ◽  
Vol 118 (2) ◽  
pp. 352-357 ◽  
Author(s):  
Satoru Ushijima ◽  
Nobukazu Tanaka
Keyword(s):  
Particle Tracking ◽  
High Speed ◽  
Particle Tracking Velocimetry ◽  
Laser Light ◽  
Three Dimensional ◽  
Numerical Procedure ◽  
Light Sheet ◽  
Optical Scanners ◽  
Particle Images

This paper describes three-dimensional particle tracking velocimetry (3D PTV), which enables us to obtain remarkably larger number of velocity vectors than previous techniques. Instead of the usual stereoscopic image recordings, the present 3D PTV visualizes an entire three-dimensional flow with the scanning laser-light sheets generated from a pair of optical scanners and the images are taken by a high-speed video system synchronized with the scannings. The digital image analyses to derive velocity components are based on the numerical procedure (Ushijima and Tanaka, 1994), in which several improvements have been made on the extraction of particle images, the determination of their positions, the derivation of velocity components and others. The present 3D PTV was applied to the rotating fluids, accompanied by Ekman boundary layers, and their complicated secondary flow patterns, as well as the primary circulations, are quantitatively captured.

High-Speed, High-Resolution 3D Imaging Using Projector Defocusing

2011 ◽  
pp. 121-140
Author(s):  
Song Zhang ◽  
Yuanzheng Gong
Keyword(s):  
High Speed ◽  
3D Imaging ◽  
Imaging Techniques ◽  
Three Dimensional ◽  
Projection System ◽  
Digital Fringe Projection ◽  
System Nonlinearity ◽  
Projection Techniques ◽  
Fringe Patterns ◽  
3D Scene

With the advance of software and hardware, three-dimensional (3D) scene digitization becomes increasingly important. Over the years, numerous 3D imaging techniques have been developed. Among these techniques, the methods based on analyzing sinusoidal structured (fringe) patterns stand out due to their achievable speed and resolution. With the development of digital video display technologies, digital fringe projection techniques emerge as a mainstream for 3D imaging. However, developing such a system is not easy especially when an off-the-shelf projector is used. The major challenging problems are: (1) the projection system nonlinearity; (2) the precise synchronization requirement; and (3) the projection system speed limit. This chapter will present an alternative route for 3D imaging while reducing these problems. The fundamentals of the proposed technique will be introduced, the analytical and experimental results will be shown, and its advantages and limitations will be addressed.

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