Approach to Design Loading Protocols for Cartilage Tissue Engineering: Hypothesis, Experiment and Model

2010 ◽  
Author(s):  
C. C. van Donkelaar ◽  
M. Khoshgoftar ◽  
L. M. Kock ◽  
K. Ito
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Native Cartilage ◽  
Native Collagen ◽  
Nutritional Effects ◽  
Proteoglycan Content ◽  
Gradual Variation ◽  
Engineered Cartilage

Tissue engineered cartilage has reached the level of maturity where the cells, either chondrocytes, BMSC’s or other cells are stimulated to produce a tissue of which the biochemical content qualitatively resembles that of native cartilage. Quantitatively, the proteoglycan content approaches that of native content in long term cultures, but to obtain native collagen fractions is still challenging. Engineered cartilage matrix is either homogeneously distributed, or shows gradual variation from the periphery to the center, caused by nutritional effects.

The Response of Tissue Engineered Cartilage to the Temporal Application of Transforming and Insulin-Like Growth Factors

2007 ◽  
Author(s):  
Christopher J. O’Conor ◽  
Kenneth W. Ng ◽  
Lindsay E. Kugler ◽  
Gerard A. Ateshian ◽  
Clark T. Hung
Keyword(s):  
Tissue Engineering ◽  
Growth Factors ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Mechanical Stimuli ◽  
Tissue Development ◽  
Engineering Research ◽  
Engineered Cartilage

Agarose has been used as an experimental scaffold for cartilage tissue engineering research due to its biocompatibility with chondrocytes, support of cartilage tissue development, and ability to transmit mechanical stimuli [1–3]. Tissue engineering studies have demonstrated that the temporal application of transforming growth factor (TGF) β3 for only 2 weeks elicits rapid tissue development that results in mechanical properties approaching native values [4]. However, it is not known whether this response to a 2-week exposure to growth factors is unique to TGF-β3. Therefore, the present study characterizes the response of tissue engineered cartilage to the temporal application of the anabolic growth factors TGF-β1, TGF-β3, and insulin-like growth factor I (IGF-I).

Strains in Stratified Agarose Constructs as Determined by Displacement-Encoded MRI

2012 ◽  
Author(s):  
Adam Griebel ◽  
C. C. van Donkelaar ◽  
Corey P. Neu
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Mechanical Stimuli ◽  
Healthy Cartilage ◽  
Mechanical Quality ◽  
Different Strains ◽  
Engineered Cartilage ◽  
External Stimuli

Osteoarthritis (OA) is a debilitating disease for which no satisfactory treatment exists. Tissue engineering-based strategies have shown considerable potential for repair. Agarose is frequently used as a scaffold material, as chondrocytes maintain their phenotype and cells remain responsive to mechanical stimuli. To improve the mechanical quality of tissue engineered cartilage, recent studies aimed to reproduce the depth-dependent structure of healthy cartilage. One approach to achieve this is by applying depth-dependent mechanical stimuli via cyclically sliding a glass cylinder over the cell-seeded agarose construct [1,2]. The different strains applied to the surface and the deeper regions are expected to induce stratified matrix synthesis and therefore stratified tissue stiffness. Consequently, with the same external stimuli, the internal strain distribution may alter with ongoing tissue development. Such effect is important to understand in order to optimize mechanical loading regimes for cartilage tissue engineering.

scholarly journals Carboxymethyl Cellulose Entrapped in a Poly(vinyl) Alcohol Network: Plant-Based Scaffolds for Cartilage Tissue Engineering

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 578
Author(s):  
Jirapat Namkaew ◽  
Panitporn Laowpanitchakorn ◽  
Nuttapong Sawaddee ◽  
Sirinee Jirajessada ◽  
Sittisak Honsawek ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Carboxymethyl Cellulose ◽  
Cell Attachment ◽  
Cartilage Tissue Engineering ◽  
Crosslinking Density ◽  
Cartilage Tissue ◽  
Poly Vinyl Alcohol ◽  
Porous Scaffolds ◽  
Swelling Properties ◽  
Engineered Cartilage

Cartilage has a limited inherent healing capacity after injury, due to a lack of direct blood supply and low cell density. Tissue engineering in conjunction with biomaterials holds promise for generating cartilage substitutes that withstand stress in joints. A major challenge of tissue substitution is creating a functional framework to support cartilage tissue formation. Polyvinyl alcohol (PVA) was crosslinked with glutaraldehyde (GA), by varying the mole ratios of GA/PVA in the presence of different amounts of plant-derived carboxymethyl cellulose (CMC). Porous scaffolds were created by the freeze-drying technique. The goal of this study was to investigate how CMC incorporation and crosslinking density might affect scaffold pore formation, swelling behaviors, mechanical properties, and potential use for engineered cartilage. The peak at 1599 cm−1 of the C=O group in ATR–FTIR indicates the incorporation of CMC into the scaffold. The glass transition temperature (Tg) and Young’s modulus were lower in the PVA/CMC scaffold, as compared to the PVA control scaffold. The addition of CMC modulates the pore architecture and increases the swelling ratio of scaffolds. The toxicity of the scaffolds and cell attachment were tested. The results suggest that PVA/CMC scaffolding material can be tailored in terms of its physical and swelling properties to potentially support cartilage formation.

scholarly journals Fabrication and In Vitro Study of Tissue-Engineered Cartilage Scaffold Derived from Wharton’s Jelly Extracellular Matrix

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Tongguang Xiao ◽  
Weimin Guo ◽  
Mingxue Chen ◽  
Chunxiang Hao ◽  
Shuang Gao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Articular Cartilage ◽  
Growth Factor ◽  
Cartilage Tissue Engineering ◽  
Wharton’S Jelly ◽  
Immunofluorescent Staining ◽  
Cartilage Tissue ◽  
Wharton's Jelly ◽  
Engineered Cartilage

The scaffold is a key element in cartilage tissue engineering. The components of Wharton’s jelly are similar to those of articular cartilage and it also contains some chondrogenic growth factors, such as insulin-like growth factor I and transforming growth factor-β. We fabricated a tissue-engineered cartilage scaffold derived from Wharton’s jelly extracellular matrix (WJECM) and compared it with a scaffold derived from articular cartilage ECM (ACECM) using freeze-drying. The results demonstrated that both WJECM and ACECM scaffolds possessed favorable pore sizes and porosities; moreover, they showed good water uptake ratios and compressive moduli. Histological staining confirmed that the WJECM and ACECM scaffolds contained similar ECM. Moreover, both scaffolds showed good cellular adherence, bioactivity, and biocompatibility. MTT and DNA content assessments confirmed that the ACECM scaffold tended to be more beneficial for improving cell proliferation than the WJECM scaffold. However, RT-qPCR results demonstrated that the WJECM scaffold was more favorable to enhance cellular chondrogenesis than the ACECM scaffold, showing more collagen II and aggrecan mRNA expression. These results were confirmed indirectly by glycosaminoglycan and collagen content assessments and partially confirmed by histology and immunofluorescent staining. In conclusion, these results suggest that a WJECM scaffold may be favorable for future cartilage tissue engineering.

scholarly journals Role of dexamethasone in the long-term functional maturation of MSC-laden hyaluronic acid hydrogels for cartilage tissue engineering

2017 ◽  
Vol 36 (6) ◽  
pp. 1717-1727 ◽  
Author(s):  
Minwook Kim ◽  
Sean T. Garrity ◽  
David R. Steinberg ◽  
George R. Dodge ◽  
Robert L. Mauck
Keyword(s):  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Functional Maturation

scholarly journals CARTILAGE TISSUE ENGINEERING

2005 ◽  
Vol 17 (02) ◽  
pp. 61-71 ◽  
Author(s):  
CHIH-HUNG CHANG ◽  
FENG-HUEI LIN ◽  
TZONG-FU KUO ◽  
HWA-CHANG LIU
Keyword(s):  
Tissue Engineering ◽  
Animal Studies ◽  
Cartilage Tissue Engineering ◽  
In Vivo Studies ◽  
Cartilage Tissue ◽  
Current Status ◽  
Materials Used ◽  
Engineered Cartilage

Tissue engineering is a new approach for articular cartilage repair. The aim of the present article was to review the current status of cartilage tissue engineering researches. The scaffold materials used for cartilage tissue engineering, the in vitro, in vivo studies and the clinical trials were all reviewed. Our researches about in vitro cartilage tissue engineering with new type bioactive scaffold and preliminary animal studies results will also be described. The scaffold was tricopolymer made from gelatin, hyaluronan and chondroitin. Chondrocytes seeded in tricopolymer showed in vitro engineered cartilage formation. The engineered cartilage constructs were implanted into knee joints of miniature pigs for animal study.

scholarly journals 173 LONG-TERM STUDY OF STRATIFIED ALGINATE CONSTRUCT FOR CARTILAGE TISSUE ENGINEERING

2010 ◽  
Vol 18 ◽  
pp. S84
Author(s):  
J. Schiavi-Tritz ◽  
N. Charif ◽  
N. de Isla ◽  
R. Rahouadj ◽  
A. Pinzano ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Term Study ◽  
Long Term Study

Binding and Release Kinetics of Glycosaminoglycans and Collagen in Engineered Cartilage Under TGF-β Supplementation

2013 ◽  
Author(s):  
Robert J. Nims ◽  
Alexander D. Cigan ◽  
Michael B. Albro ◽  
Clark T. Hung ◽  
Gerard A. Ateshian
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Release Kinetics ◽  
Cartilage Tissue Engineering ◽  
Type Ii Collagen ◽  
Cartilage Tissue ◽  
Type Ii ◽  
Matrix Products ◽  
Engineered Cartilage ◽  
Kinetics Of

Cartilage tissue engineering (CTE) is a strategy of great interest and promise for the replacement of osteoarthritic (OA) cartilage. In CTE, chondrocytes are used to synthesize cartilage matrix products (predominantly glycosaminoglycans (GAG) and type II collagen). The aim for CTE is to develop engineered constructs with mechanical properties and biochemical composition comparable to native tissue, to reproduce its functional properties.

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