scholarly journals ISEcp1-Mediated Transposition and Homologous Recombination Can Explain the Context of blaCTX-M-62 Linked to qnrB2

2010 ◽  
Vol 54 (7) ◽  
pp. 3039-3042 ◽  
Author(s):  
Zhiyong Zong ◽  
Sally R. Partridge ◽  
Jonathan R. Iredell
Keyword(s):  
Homologous Recombination ◽  
Klebsiella Pneumoniae ◽  
Conjugative Plasmid ◽  
Adjacent Gene ◽  
Class 1 Integron ◽  
Class 1

ABSTRACT bla CTX-M-62, a C508T variant of bla CTX-M-3b, was transferred from Klebsiella pneumoniae JIE137 on a conjugative plasmid together with a class 1 integron containing the dfrA12-gcuF-aadA2 cassette array, ISCR1, and qnrB2. bla CTX-M-62 lies between intact and rearranged copies of ISEcp1 in a configuration that can be explained by a combination of transposition and homologous recombination and which also illustrates the ability of ISEcp1 to mobilize an adjacent gene as part of transposition units of different sizes.

scholarly journals Probable Interspecies Transfer of the blaVIM-4 Gene between Enterobacter cloacae and Klebsiella pneumoniae in a Single Infant Patient

2015 ◽  
Vol 64 (4) ◽  
pp. 387-389 ◽  
Author(s):  
Katarzyna Piekarska ◽  
Katarzyna Zacharczuk ◽  
Magdalena Rzeczkowska ◽  
Tomasz Wołkowicz ◽  
Aleksandra Januszkiewicz ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Nucleotide Sequence ◽  
Klebsiella Pneumoniae ◽  
Enterobacter Cloacae ◽  
Conjugative Plasmid ◽  
High Homology ◽  
Class 1 Integron ◽  
Potential Source ◽  
Class 1 ◽  
Show Evidence

We report the interspecies transfer of the blaVIM-4 gene in MBL-producing Enterobacter cloacae and Klebsiella pneumoniae isolates from a newborn patient who had received meropenem therapy. We show evidence that gene blaVIM-4 was transmitted as a part of the class‑1 integron on a ca. ~90 kb conjugative plasmid. High homology of nucleotide sequence was observed between the integron found in VIM-4 producing E. cloacae and K. pneumoniae strains tested and class‑1 integrons previously reporteded in Pseudomonas aeruginosa from Hungary and Poland. This finding may suggest P. aeruginosa as a potential source of acquired VIM-4 in Enterobacteriaceae.

scholarly journals In34, a Complex In5 Family Class 1 Integron Containing orf513 and dfrA10

2003 ◽  
Vol 47 (1) ◽  
pp. 342-349 ◽  
Author(s):  
Sally R. Partridge ◽  
Ruth M. Hall
Keyword(s):  
Homologous Recombination ◽  
Resistance Genes ◽  
Multiple Drug Resistance ◽  
Gene Cassette ◽  
Multidrug Resistant ◽  
Conjugative Plasmid ◽  
Common Region ◽  
Class 1 Integron ◽  
Class 1 ◽  
The Common

ABSTRACT A complex class 1 integron, In34, found in a conjugative plasmid from a multidrug-resistant Klebsiella pneumoniae strain isolated in 1997 at a hospital in Sydney, Australia, was shown to have a backbone related to that of In2, which belongs to the In5 family. In In34, the aadB gene cassette replaces the aadA1a cassette in In2, and two additional resistance genes, dfrA10 and aphA1, that are not part of a gene cassette are present. The aphA1 gene is in a Tn4352-like transposon that is located in the tniA gene. The dfrA10 gene lies adjacent to a 2,154-bp DNA segment, known as the common region, that contains an open reading frame predicting a product of 513 amino acids (Orf513). Orf513 is 66 and 55% identical to the products of two further open reading frames that, like the common region, are found adjacent to antibiotic resistance genes. A 27-bp conserved sequence was found at one end of each type of common region. The loss of dfrA10 due to homologous recombination between flanking direct repeats and incorporation of the excised circle by homologous recombination were demonstrated. Part of In34 is identical to the sequenced portion of In7, which is from a multidrug-resistant Escherichia coli strain that had been isolated 19 years earlier in the same hospital. In34 and In7 are in plasmids that contain the same six resistance genes conferring resistance to ampicillin, chloramphenicol, gentamicin, kanamycin, neomycin, tobramycin, trimethoprim, and sulfonamides, but the plasmid backbones appear to be unrelated, suggesting that translocation of a multiple-drug-resistance-determining region as well as horizontal transfer may have occurred.

scholarly journals Salmonella enterica Serovar Typhi Strains Isolated in Korea Containing a Multidrug Resistance Class 1 Integron

2003 ◽  
Vol 47 (6) ◽  
pp. 2006-2008 ◽  
Author(s):  
Hyunjoo Pai ◽  
Jeong-hum Byeon ◽  
Sunmi Yu ◽  
Bok Kwon Lee ◽  
Shukho Kim
Keyword(s):  
Multidrug Resistance ◽  
Resistance Genes ◽  
Salmonella Enterica ◽  
Multidrug Resistant ◽  
Conjugative Plasmid ◽  
The Novel ◽  
Class 1 Integron ◽  
Salmonella Enterica Serovar Typhi ◽  
Class 1 ◽  
Clonal Spread

ABSTRACT Six strains of Salmonella enterica serovar Typhi which were resistant to ampicillin, chloramphenicol, trimethoprim-sulfamethoxazole, streptomycin, tetracycline, and gentamicin were isolated in Korea. This multidrug resistance was transferred by a conjugative plasmid of about 50 kb. The plasmid harbored a class 1 integron, which included six resistance genes, aacA4b, catB8, aadA1, dfrA1, aac(6′)-IIa, and the novel blaP2, in that order. All of the isolates showed the same-size plasmids and the same ribotyping patterns, which suggests a clonal spread of these multidrug-resistant isolates.

Molecular Detection of Class 1 Integron-Associated Gene Cassettes in KPC-2-Producing Klebsiella pneumoniae Clones by Whole-Genome Sequencing

2019 ◽  
Vol 25 (8) ◽  
pp. 1127-1131 ◽  
Author(s):  
Bruna Fuga ◽  
Sabrina Royer ◽  
Paola Amaral de Campos ◽  
Melina Lorraine Ferreira ◽  
Iara Rossi ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Molecular Detection ◽  
Whole Genome ◽  
Class 1 Integron ◽  
Gene Cassettes ◽  
Class 1

scholarly journals First Detection of a Carbapenem-Hydrolyzing Metalloenzyme in Two Enterobacteriaceae Isolates in Spain

2005 ◽  
Vol 49 (8) ◽  
pp. 3492-3494 ◽  
Author(s):  
M. Teresa Tórtola ◽  
Susana Lavilla ◽  
Elisenda Miró ◽  
Juan José González ◽  
Nieves Larrosa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Gene Cassette ◽  
Class 1 Integron ◽  
Class 1 ◽  
First Time

ABSTRACT Two strains of Enterobacteriaceae, Escherichia coli and Klebsiella pneumoniae, producing VIM-1 were isolated for the first time in Spain. In both strains, bla VIM-1 was found to be carried on a gene cassette inserted into a class 1 integron. The bla VIM-1-containing integron was located on a transferable plasmid.

scholarly journals Characterization of an IncR Plasmid with Two Copies of ISCR-Linked qnrB6 from ST968 Klebsiella pneumoniae

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Changrui Qian ◽  
Xinyi Zhu ◽  
Junwan Lu ◽  
Kai Shen ◽  
Qianqian Chen ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Resistance Genes ◽  
Genomic Analysis ◽  
The Other ◽  
Comparative Genomic ◽  
Clear Understanding ◽  
Class 1 Integron ◽  
Bacterial Chromosomes ◽  
Class 1 ◽  
Plasmid Backbone

To characterize the molecular structure of IncR plasmid-related sequences, comparative genomic analysis was conducted using 261 IncR plasmid backbone-related sequences. Among the sequences, 257 were IncR plasmids including the multidrug-resistance IncR plasmid pR50-74 from Klebsiella pneumoniae strain R50 of this work, and the other four were from bacterial chromosomes. The IncR plasmids were derived from different bacterial genera or species, mainly Klebsiella pneumoniae (70.82%, 182/257), Escherichia coli (11.28%, 29/257), Enterobacter cloacae (7.00%, 18/257), and Citrobacter freundii (3.50%, 9/257). The bacterial chromosomes carrying IncR plasmid backbone sequences were derived from Proteus mirabilis AOUC-001 and Klebsiella pneumoniae KPN1344, among others. The IncR backbone sequence of P. mirabilis AOUC-001 chromosome shows the highest identity with that of pR50-74. Complex class 1 integrons carrying various copies of ISCR1-sdr-qnrB6-△qacE/sul1 (ISCR1-linked qnrB6 unit) were identified in IncR plasmids. In addition to two consecutive copies of qnrB6-qacE-sul1, the other resistance genes encoded on pR50-74 are all related to mobile genetic elements, such as IS1006, IS26, and the class 1 integron. This study provides a clear understanding of the mobility and plasticity of the IncR plasmid backbone sequence and emphasizes the important role of ISCR in the recruitment of multicopy resistance genes.

scholarly journals Complex Multiple Antibiotic and Mercury Resistance Region Derived from the r-det of NR1 (R100)

2004 ◽  
Vol 48 (11) ◽  
pp. 4250-4255 ◽  
Author(s):  
Sally R. Partridge ◽  
Ruth M. Hall
Keyword(s):  
Homologous Recombination ◽  
Shigella Flexneri ◽  
Mercury Resistance ◽  
Close Relative ◽  
Inverted Repeats ◽  
Large Plasmid ◽  
Class 1 Integron ◽  
Class 1 ◽  
New Location ◽  
Adjacent Sequence

ABSTRACT The sequence of the 45.2-kb multidrug and mercury resistance region of pRMH760, a large plasmid from a clinical isolate of Klebsiella pneumoniae collected in 1997 in Australia, was completed. Most of the modules found in the resistance determinant (r-det), or Tn2670, region of NR1 (also known as R100), isolated from a Shigella flexneri strain in Japan in the late 1950s, were present in pRMH760 but in a different configuration. The location was also different, with the Tn2670-derived region flanked by the transposition module of Tn1696 and a mercury resistance module almost identical to one found in the plasmid pDU1358. This arrangement is consistent with a three-step process. First, the r-det was circularized via homologous recombination between the IS1 elements and reincorporated at a new location, possibly in a different plasmid, via homologous recombination between the 5′-conserved (5′-CS) or 3′-CS of the In34 integron in the r-det and the same region of a second class 1 integron in a Tn1696 relative. Subsequently, resolvase-mediated recombination between the res sites in the r-det and a second mercury resistance transposon removed one end of the Tn1696-like transposon and part of the second transposon. Other events occurring within the r-det-derived portion have also contributed to the formation of the pRMH760 resistance region. Tn2 or a close relative that includes the bla TEM-1b gene had moved into the Tn21 mercury resistance module with subsequent deletion of the adjacent sequence, and all four 38-bp inverted repeats corresponding to Tn21 family transposon termini have been interrupted by an IS4321-like element.

scholarly journals Characterization of multidrug-resistant and extended-spectrum β-lactamase-producing Klebsiella pneumoniae strains from Malaysian hospitals

2009 ◽  
Vol 58 (11) ◽  
pp. 1463-1469 ◽  
Author(s):  
King Ting Lim ◽  
Chew Chieng Yeo ◽  
Rohani Md Yasin ◽  
Ganeswrie Balan ◽  
Kwai Lin Thong
Keyword(s):  
Klebsiella Pneumoniae ◽  
Dna Fingerprinting ◽  
Antibiotic Resistance Gene ◽  
Multidrug Resistant ◽  
Pcr Detection ◽  
Management Problem ◽  
Class 1 Integron ◽  
Extended Spectrum ◽  
Class 1 ◽  
Encoding Genes

The emergence of multidrug-resistant (MDR) and extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae poses a serious antibiotic management problem as resistance genes are easily transferred from one organism to another. Fifty-one strains of K. pneumoniae isolated from sporadic cases in various hospitals throughout Malaysia were analysed by antimicrobial susceptibility testing, PCR detection of ESBL-encoding genes and DNA fingerprinting. Although 27 of the 51 K. pneumoniae strains were MDR (i.e. resistant to three or more classes of antibiotics), the majority of the strains (98 %) were sensitive to imipenem. PCR detection using ESBL gene-specific primers showed that 46 of the K. pneumoniae strains harboured bla SHV, 19 harboured bla CTX-M, 5 harboured bla OXA-1 and 4 harboured bla TEM-1. Class 1 integron-encoded intI1 integrase was detected in 21 of the 51 K. pneumoniae strains and amplification of the integron 5′CS region showed the presence of several known antibiotic resistance gene cassettes of various sizes. Results of conjugation and transformation experiments indicated that some of the ESBL-encoding genes (i.e. bla SHV, bla CTX-M and bla TEM-1) were transmissible and were likely plasmid-encoded. DNA fingerprinting using PFGE and PCR-based methods indicated that the 51 K. pneumoniae strains were genetically diverse and heterogeneous.

scholarly journals Klebsiella pneumoniae blaNDM-1 carrying a class 1 integron causing a hospital outbreak in a Mexican attention center

2021 ◽  
Vol 15 (05) ◽  
pp. 657-664
Author(s):  
Iliana Alejandra Cortés-Ortíz ◽  
Julio Cesar Juárez-Gómez ◽  
Concepción Cu-Quijano ◽  
Rocio Flores-Paz ◽  
Emilio Mariano Durán-Manuel ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Variable Region ◽  
Epidemiological Surveillance ◽  
Resistant Bacteria ◽  
Rrna Gene ◽  
High Morbidity ◽  
Class 1 Integron ◽  
Genetic Characteristics ◽  
Extended Spectrum ◽  
Class 1

Introduction: Infections acquired in hospitals are the cause of high morbidity and mortality and with the emergence of resistant bacteria, the problem is greater. The aim of this work was to determine the genetic characteristics and timeline of Klebsiella pneumoniae blaNDM-1 carrying a class 1 integron involved in an intrahospital outbreak. Methodology: Investigation was made from the first detection of K. pneumoniae blaNDM-1, strain “466”, and the last clone “423”. 16S rRNA gene analysis showed that 466 strain and clones were related to K. pneumoniae. Extended-spectrum β-lactamases (ESBL) was detected according to the Clinical and Laboratory Standards Institute (CLSI) and real time-PCR. Typing of K. pneumoniae blaNDM-1 strains was carried by ERIC-PCR and sequencing the variable region of the integrons were performed. Results: A cluster of six resistant isolates of K. pneumoniae blaNDM-1 was detected in intensive care unit (ICU), internal medicine (IM) and orthopedics (OT). Timeline revealed that the first bacterial identification was in ICU and the last clone in OT service. The array genetic of variable region was “IntI/aadA5-drfA17/qacEΔ1-Sul1”. Conclusions: The evidences highlight the importance of the epidemiological surveillance of Extended-spectrum β-lactamases (ESBL) strains, as well as the need for molecular epidemiological studies to identify the routes of transmission and the contamination sources within health personnel.

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