scholarly journals Molecular Characterization of Escherichia coli Isolates Carrying mcr-1, fosA3, and Extended-Spectrum-β-Lactamase Genes from Food Samples in China

2017 ◽  
Vol 61 (6) ◽  
Author(s):  
Xiaobo Liu ◽  
Ruichao Li ◽  
Zhiwei Zheng ◽  
Kaichao Chen ◽  
Miaomiao Xie ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Molecular Characterization ◽  
Food Products ◽  
Multidrug Resistant ◽  
Food Samples ◽  
Content Type ◽  
E Coli ◽  
Extended Spectrum

ABSTRACT This study surveyed the prevalence of mcr-1 in extended-spectrum-β-lactamase (ESBL)-producing Escherichia coli strains of food origin in China and identified strains that carried mcr-1, fosA3, and ESBL genes, which were carried in various plasmids. The mcr-1 and ESBL genes could be cotransferred by one or more types of plasmids. The presence of these multidrug-resistant E. coli strains in food products might pose a huge threat to public health.

Characterization of extended-spectrum and CMY-2 ß-lactamases, and associated virulence genes in Escherichia coli from food of animal origin in México

2018 ◽  
Vol 120 (7) ◽  
pp. 1457-1473 ◽  
Author(s):  
Edwin Barrios-Villa ◽  
Gerardo Cortés-Cortés ◽  
Patricia Lozano Zarain ◽  
Sergio Romero-Romero ◽  
Norarizbeth Lara Flores ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Virulence Genes ◽  
Food Samples ◽  
Animal Origin ◽  
Content Type ◽  
E Coli ◽  
Extended Spectrum ◽  
Food Of Animal Origin ◽  
Cephalosporin Resistance

Purpose Broad-spectrum cephalosporin resistance is rapidly increasing in Escherichia coli, representing a food safety problem. The purpose of this paper is to characterize eight extended-spectrum-ß-lactamase (ESBL) and acquired AmpC ß-lactamase-producing E. coli isolates and virotypes associated, obtained from chicken and pork food samples in Puebla, Mexico. Design/methodology/approach Samples (36 from chicken and 10 from pork) were cultured on Levine agar plates supplemented with cefotaxime (2 mg/L) for isolation of cefotaxime-resistant (CTXR) E. coli. CTXR-E. coli isolates were detected in 33 of 46 samples (72 percent), and one isolate/sample was characterized (28 from chicken and 5 from pork), for ESBL production, phylogenetic group, sequence typing, resistance and virulence genes by PCR and sequencing. Findings Results showed 16 ESBL-E. coli (35 percent) (12/16 belonging to phylogroup B1) and 8 CMY-2-E. coli (17 percent). ESBL detected were as follows (number of isolates): CTX-M-2 (8); CTX-M-1 (2); CTX-M-15 (1); SHV-2a (4) and TEM-52c (1). In total, 20 different sequence types (STs) were identified among the ESBL- or CMY-2-producing E. coli strains, which included four new ones. The CTX-M-15 β-lactamase was detected in one E. coli ST617-ST10 Cplx-B1 strain that also carried ibeA gene. One CMY-2-positive strain of lineage ST224-B2 was detected and it carried the qnrA1 gene. Originality/value In this study, a ST131-based virotyping scheme for strains from food of animal origin was established since this kind of strains constitutes an important vehicle of virulent ESBL- and CMY-2-producing E. coli isolates, which could be transmitted to humans by direct contact or through the food chain.

scholarly journals Houseflies (Musca domestica) as Vectors for Extended-Spectrum β-Lactamase-Producing Escherichia coli on Spanish Broiler Farms

2015 ◽  
Vol 81 (11) ◽  
pp. 3604-3611 ◽  
Author(s):  
Marc Solà-Ginés ◽  
Juan José González-López ◽  
Karla Cameron-Veas ◽  
Nuria Piedra-Carrasco ◽  
Marta Cerdà-Cuéllar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Ecological Niches ◽  
Potential Contribution ◽  
Content Type ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum

ABSTRACTFlies may act as potential vectors for the spread of resistant bacteria to different environments. This study was intended to evaluate the presence ofEscherichia colistrains resistant to cephalosporins in flies captured in the areas surrounding five broiler farms. Phenotypic and molecular characterization of the resistant population was performed by different methods: MIC determination, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and phylotyping. The presence of extended-spectrum beta-lactamase (ESBL) genes, their plasmid location, and the mobile genetic elements involved in their mobilization were studied. Additionally, the presence of 35 genes associated with virulence was evaluated. Out of 682 flies captured, 42 yielded ESBL-producingE. coli. Of these isolates, 23 containedblaCTX-M-1, 18 containedblaCTX-M-14, and 1 containedblaCTX-M-9. ESBL genes were associated mainly with the presence of the IncI1 and IncFIB replicons. Additionally, all the strains were multiresistant, and five of them also harboredqnrS. Identical PFGE profiles were found forE. coliisolates obtained from flies at different sampling times, indicating a persistence of the same clones in the farm environment over months. According to their virulence genes, 81% of the isolates were considered avian-pathogenicE. coli(APEC) and 29% were considered extraintestinal pathogenicE. coli(ExPEC). The entrance of flies into broiler houses constitutes a considerable risk for colonization of broilers with multidrug-resistantE. coli. ESBLs in flies reflect the contamination status of the farm environment. Additionally, this study demonstrates the potential contribution of flies to the dissemination of virulence and resistance genes into different ecological niches.

scholarly journals Characterization of Multidrug-Resistant Escherichia coli Isolates from Animals Presenting at a University Veterinary Hospital

2011 ◽  
Vol 77 (20) ◽  
pp. 7104-7112 ◽  
Author(s):  
Maria Karczmarczyk ◽  
Yvonne Abbott ◽  
Ciara Walsh ◽  
Nola Leonard ◽  
Séamus Fanning
Keyword(s):  
Escherichia Coli ◽  
Molecular Mechanisms ◽  
Gene Array ◽  
Multidrug Resistant ◽  
Genetic Determinants ◽  
Gene Encoding ◽  
Content Type ◽  
E Coli ◽  
Class 1

ABSTRACTIn this study, we examined molecular mechanisms associated with multidrug resistance (MDR) in a collection ofEscherichia coliisolates recovered from hospitalized animals in Ireland. PCR and DNA sequencing were used to identify genes associated with resistance. Class 1 integrons were prevalent (94.6%) and contained gene cassettes recognized previously and implicated mainly in resistance to aminoglycosides, β-lactams, and trimethoprim (aadA1,dfrA1-aadA1,dfrA17-aadA5,dfrA12-orfF-aadA2,blaOXA-30-aadA1,aacC1-orf1-orf2-aadA1,dfr7). Class 2 integrons (13.5%) contained thedfrA1-sat1-aadA1gene array. The most frequently occurring phenotypes included resistance to ampicillin (97.3%), chloramphenicol (75.4%), florfenicol (40.5%), gentamicin (54%), neomycin (43.2%), streptomycin (97.3%), sulfonamide (98.6%), and tetracycline (100%). The associated resistance determinants detected includedblaTEM,cat,floR,aadB,aphA1,strA-strB,sul2, andtet(B), respectively. TheblaCTX-M-2gene, encoding an extended-spectrum β-lactamase (ESβL), andblaCMY-2, encoding an AmpC-like enzyme, were identified in 8 and 18 isolates, respectively. The mobility of the resistance genes was demonstrated using conjugation assays with a representative selection of isolates. High-molecular-weight plasmids were found to be responsible for resistance to multiple antimicrobial compounds. The study demonstrated that animal-associated commensalE. coliisolates possess a diverse repertoire of transferable genetic determinants. Emergence of ESβLs and AmpC-like enzymes is particularly significant. To our knowledge, theblaCTX-M-2gene has not previously been reported in Ireland.

scholarly journals Molecular Characterization of UpaB and UpaC, Two New Autotransporter Proteins of Uropathogenic Escherichia coli CFT073

2011 ◽  
Vol 80 (1) ◽  
pp. 321-332 ◽  
Author(s):  
Luke P. Allsopp ◽  
Christophe Beloin ◽  
Glen C. Ulett ◽  
Jaione Valle ◽  
Makrina Totsika ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Molecular Characterization ◽  
Host Responses ◽  
Upec Strain ◽  
Content Type ◽  
Pcr Screening ◽  
E Coli ◽  
K 12 ◽  
Encoding Genes

ABSTRACTUropathogenicEscherichia coli(UPEC) is the primary cause of urinary tract infection (UTI) in the developed world. The major factors associated with virulence of UPEC are fimbrial adhesins, which mediate specific attachment to host receptors and trigger innate host responses. Another group of adhesins is represented by the autotransporter (AT) subgroup of proteins. The genome-sequenced prototype UPEC strain CFT073 contains 11 putative AT-encoding genes. In this study, we have performed a detailed molecular characterization of two closely related AT adhesins from CFT073: UpaB (c0426) and UpaC (c0478). PCR screening revealed that theupaBandupaCAT-encoding genes are common inE. coli. TheupaBandupaCgenes were cloned and characterized in a recombinantE. coliK-12 strain background. This revealed that they encode proteins located at the cell surface but possess different functional properties: UpaB mediates adherence to several ECM proteins, while UpaC expression is associated with increased biofilm formation. In CFT073,upaBis expressed whileupaCis transcriptionally repressed by the global regulator H-NS. In competitive colonization experiments employing the mouse UTI model, CFT073 significantly outcompeted itsupaB(but notupaC) isogenic mutant strain in the bladder. This attenuated phenotype was also observed in single-challenge experiments, where deletion of theupaBgene in CFT073 significantly reduced early colonization of the bladder.

scholarly journals Molecular Characterization of qnrVC Genes and Their Novel Alleles in Vibrio spp. Isolated from Food Products in China

2018 ◽  
Vol 62 (7) ◽  
pp. e00529-18 ◽  
Author(s):  
Yanyan Zhang ◽  
Zhiwei Zheng ◽  
Edward Wai-Chi Chan ◽  
Ning Dong ◽  
Xiaodong Xia ◽  
...  
Keyword(s):  
Public Health ◽  
Molecular Characterization ◽  
Food Products ◽  
Fluoroquinolone Resistance ◽  
Content Type ◽  
Novel Alleles ◽  
Vibrio Spp

ABSTRACT This study reports the prevalences of qnrVC genes in 74 ciprofloxacin-resistant Vibrio sp. isolates. Two novel functional qnrVC alleles, qnrVC8 and qnrVC9, sharing 98% and 99% nucleotide similarity with qnrVC6 and qnrVC7, respectively, were identified. Our findings suggested that carriage of qnrVC alleles, together with target mutations in gyrA and parC genes, may contribute to the development of fluoroquinolone resistance in Vibrio species, posing a serious threat to public health.

Phenotypic and Molecular Characterization of Extended-Spectrum Beta-Lactamase Producing Escherichia coli from Poultry

2019 ◽  
Vol 15 (01) ◽  
pp. 26-30
Author(s):  
DB Barad ◽  
J H Purohit ◽  
B B Javia ◽  
H H Savsani ◽  
B S Mathapati ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Multidrug Resistant ◽  
Fourth Generation ◽  
Diffusion Method ◽  
Gujarat State ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Esbl Producers

The present study was undertaken with the objective of phenotypic and molecular characterization of extended-spectrum betalactamase (ESBL) producing E. coli isolates from poultry. A total of 300 cloacal swabs were collected, i.e., 200 from layer birds and 100 from broiler birds from three different farms in and around Junagadh district of Gujarat state. Out of 300 samples, 126 (42.00%) samples yielded E. coli. These isolates belonged to layer 85 (42.50%) and broiler 41 (41.00%) birds. Out of 126 isolates, 27 (21.43%) were confirmed as ESBL producers, i.e., 19 (22.35%) and 8 (19.51%) from layer and broiler birds, respectively, by initial screening of isolates for their susceptibility to any of the third and fourth generation cephalosporins or monobactam antibiotics by disc diffusion method and further confirmation by combination disc method using ESBL identification kits. Out of 27 phenotypically confirmed ESBL E. coli isolates blaTEM was detected in 21 (77.78%) and blaAmpC was detected in 15 (55.56%) isolates, while all the 27 isolates were found negative for the presence of blaSHV and blaCTXM genes. All the 27 isolates were found positive for either blaTEM or blaAmpC gene. Nine (33.33%) out of 27 isolates were found positive for both blaTEM and blaAmpC genes. The findings warrant the need for more strict regulations for usage of antibiotics in veterinary practices in order to prevent the emergence and dissemination of multidrug resistant E. coli pathogens among birds, animals, and humans.

scholarly journals Molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli isolates from chickens in Henan Province, China

2009 ◽  
Vol 58 (11) ◽  
pp. 1449-1453 ◽  
Author(s):  
Li Yuan ◽  
Jian-Hua Liu ◽  
Gong-Zheng Hu ◽  
Yu-Shan Pan ◽  
Zhi-Ming Liu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Molecular Characterization ◽  
Antimicrobial Agents ◽  
Animal Health ◽  
Henan Province ◽  
E Coli ◽  
Nucleotide Base ◽  
Extended Spectrum ◽  
M 14

Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli has spread rapidly worldwide and poses a serious threat to human and animal health. This study collected 51 non-replicate E. coli isolates from 14 different chicken farms in Henan Province in China from December 2007 to August 2008. The prevalence of ESBL-producing E. coli, molecular characterization of the ESBL-related bla genes, including bla TEM, bla SHV and bla CTX-M, and the susceptibilities of these bacteria to various antimicrobial agents were determined. Thirty-one of the 51 isolates were positive for an ESBL phenotype and 29 of these isolates carried one or more bla genes. Twenty-two isolates harboured bla TEM genes and 15 isolates carried bla CTX-M genes (one CTX-M-14, three CTX-M-24 and 11 CTX-M-65). One isolate carried bla TEM -57; the remaining bla TEM isolates carried bla TEM-1 with one silent nucleotide base variation (T18C). We believe that this is the first study to report TEM-57 in E. coli isolates. All isolates harbouring bla CTX-M-24 and bla CTX-M-14 and five of the bla CTX-M-65 isolates also harboured the bla TEM-1 gene. To our knowledge, this study is the first to describe detection of CTX-M-65-producing E. coli isolated from chickens. None of the isolates contained the bla SHV gene. Conjugation experiments demonstrated that bla CTX-M and bla TEM genes could be transferred to E. coli DH5α. The results indicate that ESBL frequency has reached an alarming level in chicken isolates in China, with TEM-1 and CTX-M-65 enzymes being the two predominant β-lactamases detected.

scholarly journals Diversification of Colonization Factors in a Multidrug-Resistant Escherichia coli Lineage Evolving under Negative Frequency-Dependent Selection

mBio ◽  
2019 ◽  
Vol 10 (2) ◽  
Author(s):  
Alan McNally ◽  
Teemu Kallonen ◽  
Christopher Connor ◽  
Khalil Abudahab ◽  
David M. Aanensen ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Anaerobic Metabolism ◽  
Multidrug Resistant ◽  
Frequency Dependent ◽  
Frequency Dependent Selection ◽  
Content Type ◽  
E Coli ◽  
Negative Frequency Dependent Selection ◽  
Tract Infections

ABSTRACT Escherichia coli is a major cause of bloodstream and urinary tract infections globally. The wide dissemination of multidrug-resistant (MDR) strains of extraintestinal pathogenic E. coli (ExPEC) poses a rapidly increasing public health burden due to narrowed treatment options and increased risk of failure to clear an infection. Here, we present a detailed population genomic analysis of the ExPEC ST131 clone, in which we seek explanations for its success as an emerging pathogenic strain beyond the acquisition of antimicrobial resistance (AMR) genes. We show evidence for evolution toward separate ecological niches for the main clades of ST131 and differential evolution of anaerobic metabolism, key colonization, and virulence factors. We further demonstrate that negative frequency-dependent selection acting across accessory loci is a major mechanism that has shaped the population evolution of this pathogen. IMPORTANCE Infections with multidrug-resistant (MDR) strains of Escherichia coli are a significant global public health concern. To combat these pathogens, we need a deeper understanding of how they evolved from their background populations. By understanding the processes that underpin their emergence, we can design new strategies to limit evolution of new clones and combat existing clones. By combining population genomics with modelling approaches, we show that dominant MDR clones of E. coli are under the influence of negative frequency-dependent selection, preventing them from rising to fixation in a population. Furthermore, we show that this selection acts on genes involved in anaerobic metabolism, suggesting that this key trait, and the ability to colonize human intestinal tracts, is a key step in the evolution of MDR clones of E. coli.

scholarly journals Genomic Comparison Reveals Natural Occurrence of Clinically Relevant Multidrug-Resistant Extended-Spectrum-β-Lactamase-ProducingEscherichia coliStrains

2019 ◽  
Vol 85 (13) ◽  
Author(s):  
Lin Teng ◽  
Shinyoung Lee ◽  
Amber Ginn ◽  
Sarah M. Markland ◽  
Raies A. Mir ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Negative Impact ◽  
Severe Disease ◽  
Functional Characterization ◽  
Multidrug Resistant ◽  
Natural Occurrence ◽  
Genomic Comparison ◽  
Content Type ◽  
E Coli ◽  
Extended Spectrum

ABSTRACTThe effectiveness of antibiotics has been challenged by the increasing frequency of antimicrobial resistance (AMR), which has emerged as a major threat to global health. Despite its negative impact on the development of AMR, there are few effective strategies for reducing AMR in food-producing animals. Using whole-genome sequencing and comparative genomics of 36 multidrug-resistant (MDR)Escherichia colistrains isolated from beef cattle with no previous exposure to antibiotics, we obtained results suggesting that the occurrence of MDRE. colialso arises in animals with no antibiotic selective pressure. Extended-spectrum-β-lactamase-producingE. colistrains with enhanced virulence capacities for toxin production and adherence have evolved, which implies important ramifications for animal and human health. Gene exchanges by conjugative plasmids and insertion elements have driven widespread antibiotic resistance in clinically relevant pathogens. Phylogenetic relatedness ofE. colistrains from various geographic locations and hosts, such as animals, environmental sources, and humans, suggests that transmission of MDRE. colistrains occurs intercontinentally without host barriers.IMPORTANCEMultidrug-resistant (MDR)Escherichia coliisolates pose global threats to public health due to the decreasing availability of treatment options. To better understand the characteristics of MDRE. coliisolated from food-producing animals with no antibiotic exposure, we employed genomic comparison, high-resolution phylogenetics, and functional characterization. Our findings highlight the potential capacity of MDRE. colito cause severe disease and suggest that these strains are widespread intercontinentally. This study underlines the occurrence of MDRE. coliin food-producing animals raised without antibiotic use, which has alarming, critical ramifications within animal and human medical practice.

Sign in / Sign up

Export Citation Format

Share Document