Easy Strategy To Protect Antimicrobial Peptides from Fast Degradation in Serum

ABSTRACT Antimicrobial peptides are promising novel peptide leads, but their low serum stability often limits their further consideration in drug development programs. Here, we describe a generally applicable strategy to stabilize peptides against serum proteases by replacing arginine residues with α-amino-3-guanidino-propionic acid (Agp). Peptide NH2-RRWRIVVIRVRR-CONH2 was nearby totally degraded after 8 h in mouse serum, whereas the variant with Agp substituted was degraded less than 20%. The antimicrobial activity was not affected.

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Plant antimicrobial peptides: structures, functions, and applications

Botanical Studies ◽

10.1186/s40529-021-00312-x ◽

2021 ◽

Vol 62 (1) ◽

Author(s):

Junpeng Li ◽

Shuping Hu ◽

Wei Jian ◽

Chengjian Xie ◽

Xingyong Yang

Keyword(s):

Antimicrobial Activity ◽

Antimicrobial Peptides ◽

Agricultural Production ◽

Food Additives ◽

Antimicrobial Activities ◽

Gram Positive Bacteria ◽

Potential Applications ◽

Potential Applicability ◽

Bacteria And Fungi ◽

Positively Charged

AbstractAntimicrobial peptides (AMPs) are a class of short, usually positively charged polypeptides that exist in humans, animals, and plants. Considering the increasing number of drug-resistant pathogens, the antimicrobial activity of AMPs has attracted much attention. AMPs with broad-spectrum antimicrobial activity against many gram-positive bacteria, gram-negative bacteria, and fungi are an important defensive barrier against pathogens for many organisms. With continuing research, many other physiological functions of plant AMPs have been found in addition to their antimicrobial roles, such as regulating plant growth and development and treating many diseases with high efficacy. The potential applicability of plant AMPs in agricultural production, as food additives and disease treatments, has garnered much interest. This review focuses on the types of plant AMPs, their mechanisms of action, the parameters affecting the antimicrobial activities of AMPs, and their potential applications in agricultural production, the food industry, breeding industry, and medical field.

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The effect of structural modification of antimicrobial peptides on their antimicrobial activity, hemolytic activity, and plasma stability

Journal of Peptide Science ◽

10.1002/psc.3306 ◽

2021 ◽

Author(s):

Taoran Wang ◽

Cunbin Zou ◽

Na Wen ◽

Xingdong Liu ◽

Zhao Meng ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antimicrobial Peptides ◽

Hemolytic Activity ◽

Structural Modification ◽

Plasma Stability

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Protein-Engineered Polymers Functionalized with Antimicrobial Peptides for the Development of Active Surfaces

Applied Sciences ◽

10.3390/app11125352 ◽

2021 ◽

Vol 11 (12) ◽

pp. 5352

Author(s):

Ana Margarida Pereira ◽

Diana Gomes ◽

André da Costa ◽

Simoni Campos Dias ◽

Margarida Casal ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antimicrobial Peptides ◽

Recombinant Dna ◽

Biological Properties ◽

Resistant Bacteria ◽

Recombinant Dna Technology ◽

Free Standing ◽

Microbial Cell Factories ◽

Cell Factories ◽

Antimicrobial Materials

Antibacterial resistance is a major worldwide threat due to the increasing number of infections caused by antibiotic-resistant bacteria with medical devices being a major source of these infections. This suggests the need for new antimicrobial biomaterial designs able to withstand the increasing pressure of antimicrobial resistance. Recombinant protein polymers (rPPs) are an emerging class of nature-inspired biopolymers with unique chemical, physical and biological properties. These polymers can be functionalized with antimicrobial molecules utilizing recombinant DNA technology and then produced in microbial cell factories. In this work, we report the functionalization of rPBPs based on elastin and silk-elastin with different antimicrobial peptides (AMPs). These polymers were produced in Escherichia coli, successfully purified by employing non-chromatographic processes, and used for the production of free-standing films. The antimicrobial activity of the materials was evaluated against Gram-positive and Gram-negative bacteria, and results showed that the polymers demonstrated antimicrobial activity, pointing out the potential of these biopolymers for the development of new advanced antimicrobial materials.

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2, 3-Bis(5-alkyl-2-thiono-1, 3, 5-thiadiazin-3-yl) Propionic Acid: One-PotDomino Synthesis and Antimicrobial Activity

Archiv der Pharmazie ◽

10.1002/ardp.200400906 ◽

2005 ◽

Vol 338 (1) ◽

pp. 38-43 ◽

Cited By ~ 19

Author(s):

Serry A. A. El Bialy ◽

Ali M. Abdelal ◽

Abdel-Nasser El-Shorbagi ◽

Samy M. M. Kheira

Keyword(s):

Antimicrobial Activity ◽

Propionic Acid

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Primary Structure Analysis of Antifungal Peptides from Cultivated and Wild Cereals

Plants ◽

10.3390/plants7030074 ◽

2018 ◽

Vol 7 (3) ◽

pp. 74 ◽

Cited By ~ 4

Author(s):

Eugene Rogozhin ◽

Dmitry Ryazantsev ◽

Alexey Smirnov ◽

Sergey Zavriev

Keyword(s):

Antimicrobial Activity ◽

Amino Acid ◽

Antifungal Activity ◽

Antimicrobial Peptides ◽

Structure Analysis ◽

Primary Structure ◽

Biologically Active ◽

Amino Acid Residues ◽

Wild Cereals ◽

Determining Factor

Cereal-derived bioactive peptides with antimicrobial activity have been poorly explored compared to those from dicotyledonous plants. Furthermore, there are a few reports addressing the structural differences between antimicrobial peptides (AMPs) from cultivated and wild cereals, which may shed light on significant varieties in the range and level of their antimicrobial activity. We performed a primary structure analysis of some antimicrobial peptides from wild and cultivated cereals to find out the features that are associated with the much higher antimicrobial resistance characteristic of wild plants. In this review, we identified and analyzed the main parameters determining significant antifungal activity. They relate to a high variability level in the sequences of C-terminal fragments and a high content of hydrophobic amino acid residues in the biologically active defensins in wild cereals, in contrast to AMPs from cultivated forms that usually exhibit weak, if any, activity. We analyzed the similarity of various physicochemical parameters between thionins and defensins. The presence of a high divergence on a fixed part of any polypeptide that is close to defensins could be a determining factor. For all of the currently known hevein-like peptides of cereals, we can say that the determining factor in this regard is the structure of the chitin-binding domain, and in particular, amino acid residues that are not directly involved in intermolecular interaction with chitin. The analysis of amino acid sequences of alpha-hairpinins (hairpin-like peptides) demonstrated much higher antifungal activity and more specificity of the peptides from wild cereals compared with those from wheat and corn, which may be associated with the presence of a mini cluster of positively charged amino acid residues. In addition, at least one hydrophobic residue may be responsible for binding to the components of fungal cell membranes.

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2,3-Bis(5-alkyl-2-thiono-1,3,5-thiadiazin-3-yl) Propionic Acid: One-Pot Domino Synthesis and Antimicrobial Activity.

ChemInform ◽

10.1002/chin.200522169 ◽

2005 ◽

Vol 36 (22) ◽

Author(s):

Serry A. A. El Bialy ◽

Ali M. Abdelal ◽

Abdel-Nasser El-Shorbagi ◽

Samy M. M. Kheira

Keyword(s):

Antimicrobial Activity ◽

Propionic Acid ◽

One Pot

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FDA Expedited Drug Development Programs

Journal of Pharmacovigilance ◽

10.4172/2329-6887.1000e156 ◽

2016 ◽

Vol 4 (3) ◽

Author(s):

Prathap Nagaraja Shastri

Keyword(s):

Drug Development ◽

Development Programs

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A Review of Antimicrobial Activity of Dental Mesenchymal Stromal Cells: Is There Any Potential?

Frontiers in Oral Health ◽

10.3389/froh.2021.832976 ◽

2022 ◽

Vol 2 ◽

Author(s):

Oleh Andrukhov ◽

Alice Blufstein ◽

Christian Behm

Keyword(s):

Vitamin D ◽

Antimicrobial Activity ◽

Antimicrobial Peptides ◽

Mesenchymal Stromal Cells ◽

Immune Cells ◽

Stromal Cells ◽

Multilineage Differentiation ◽

Differentiation Potential ◽

Health Maintenance ◽

Immunomodulatory Properties

Antimicrobial defense is an essential component of host-microbial homeostasis and contributes substantially to oral health maintenance. Dental mesenchymal stromal cells (MSCs) possess multilineage differentiation potential, immunomodulatory properties and play an important role in various processes like regeneration and disease progression. Recent studies show that dental MSCs might also be involved in antibacterial defense. This occurs by producing antimicrobial peptides or attracting professional phagocytic immune cells and modulating their activity. The production of antimicrobial peptides and immunomodulatory abilities of dental MSCs are enhanced by an inflammatory environment and influenced by vitamin D3. Antimicrobial peptides also have anti-inflammatory effects in dental MSCs and improve their differentiation potential. Augmentation of antibacterial efficiency of dental MSCs could broaden their clinical application in dentistry.

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FDA relations during drug development

Dialogues in Clinical Neuroscience ◽

10.31887/dcns.2000.2.3/jtmitchel ◽

2000 ◽

Vol 2 (3) ◽

pp. 213-217

Keyword(s):

Drug Development ◽

Drug Application ◽

Development Program ◽

The United States ◽

Legal Aspects ◽

Development Programs ◽

New Drug ◽

Regulatory Affairs ◽

New Drug Application ◽

Drug Development Program

Working closely and cooperatively with regulatory authorities during drug development is vital to successful drug development programs. In the United States, the drug development team includes not only members of the key disciplines of drug discovery, clinical research, regulatory affairs, marketing, chemistry, toxicology, and legal aspects, but also the Food and Drug Administration (FDA). New regulations encourage meetings at the pre-investigational new drug (pre-IND), end-of-phase-2, and pre-new drug application (pre-NDA) submission phases. Appropriate informal discussions via fax and telephone are also encouraged. By proactively interacting with the FDA, the pharmaceutical industry increases the probability of a successful drug development program.

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