scholarly journals Mosaic Tetracycline Resistance Genes Are Widespread in Human and Animal Fecal Samples

2006 ◽  
Vol 51 (3) ◽  
pp. 1115-1118 ◽  
Author(s):  
Andrea J. Patterson ◽  
Marco T. Rincon ◽  
Harry J. Flint ◽  
Karen P. Scott
Keyword(s):  
Resistance Genes ◽  
Tetracycline Resistance ◽  
Human Saliva ◽  
Fecal Samples ◽  
Tetracycline Resistance Genes ◽  
Two Samples

ABSTRACT Mosaic tetracycline resistance genes comprising tet(O), tet(W), and tet(32) sequences were abundant in DNA extracted from pig and human fecal samples, accounting for 78% (50/64) and 46% (37/80) of genes amplified with a tet(O) primer set, respectively, in two samples. The nonmosaic tet(32) gene was isolated from a human saliva bacterium.

scholarly journals Detection of a Novel, and Likely Ancestral, Tn916-Like Element from a Human Saliva Metagenomic Library

Genes ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 548
Author(s):  
Liam J. Reynolds ◽  
Muna F. Anjum ◽  
Adam P. Roberts
Keyword(s):  
Resistance Genes ◽  
Metagenomic Library ◽  
Tetracycline Resistance ◽  
Human Saliva ◽  
Fusion Event ◽  
Tetracycline Resistance Genes ◽  
Antimicrobial Resistance Genes ◽  
Genes Encoding ◽  
Gene Fusion Event ◽  
Metagenomic Approach

Tn916 is a conjugative transposon (CTn) and the first reported and most well characterised of the Tn916/Tn1545 family of CTns. Tn916-like elements have a characteristic modular structure and different members of this family have been identified based on similarities and variations in these modules. In addition to carrying genes encoding proteins required for their conjugation, Tn916-like elements also carry accessory, antimicrobial resistance genes; most commonly the tetracycline resistance gene, tet(M). Our study aimed to identify and characterise tetracycline resistance genes from the human saliva metagenome using a functional metagenomic approach. We identified a tetracycline-resistant clone, TT31, the sequencing of which revealed it to encode both tet(M) and tet(L). Comparison of the TT31 sequence with the accessory, regulation, and recombination modules of other Tn916-like elements indicated that a partial Tn916-like element encoding a truncated orf9 was cloned in TT31. Analysis indicated that a previous insertion within the truncated orf9 created the full length orf9 found in most Tn916-like transposons; demonstrating that orf9 is, in fact, the result of a gene fusion event. Thus, we hypothesise that the Tn916-like element cloned in TT31 likely represents an ancestral Tn916.

scholarly journals Antimicrobial Use and Resistance in Swine Waste Treatment Systems

2006 ◽  
Vol 72 (12) ◽  
pp. 7813-7820 ◽  
Author(s):  
Archana Jindal ◽  
Svetlana Kocherginskaya ◽  
Asma Mehboob ◽  
Matthew Robert ◽  
Roderick I. Mackie ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Waste Treatment ◽  
Growth Promotion ◽  
Tetracycline Resistance ◽  
Land Application ◽  
Molecular Methods ◽  
23S Rrna ◽  
Organic Farm ◽  
Tetracycline Resistance Genes ◽  
Fecal Streptococci

ABSTRACT Chlortetracycline and the macrolide tylosin were identified as commonly used antimicrobials for growth promotion and prophylaxis in swine production. Resistance to these antimicrobials was measured throughout the waste treatment processes at five swine farms by culture-based and molecular methods. Conventional farm samples had the highest levels of resistance with both culture-based and molecular methods and had similar levels of resistance despite differences in antimicrobial usage. The levels of resistance in organic farm samples, where no antimicrobials were used, were very low by a culture-based method targeting fecal streptococci. However, when the same samples were analyzed with a molecular method detecting methylation of a specific nucleotide in the 23S rRNA that results in resistance to macrolides, lincosamides, and streptogramin B (MLSB), an unexpectedly high level of resistant rRNA (approximately 50%) was observed, suggesting that the fecal streptococci were not an appropriate target group to evaluate resistance in the overall microbial community and that background levels of MLSB resistance may be substantial. All of the feed samples tested, including those from the organic farm, contained tetracycline resistance genes. Generally, the same tetracycline resistance genes and frequency of detection were found in the manure and lagoon samples for each commercial farm. The levels of tetracycline and MLSB resistance remained high throughout the waste treatment systems, suggesting that the potential impact of land application of treated wastes and waste treatment by-products on environmental levels of resistance should be investigated further.

Abundances of Tetracycline Resistance Genes and Tetracycline Antibiotics during Anaerobic Digestion of Swine Waste

2019 ◽  
Vol 48 (1) ◽  
pp. 171-178 ◽  
Author(s):  
Melanie Couch ◽  
Getahun E. Agga ◽  
John Kasumba ◽  
Rohan R. Parekh ◽  
John H. Loughrin ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Resistance Genes ◽  
Tetracycline Resistance ◽  
Swine Waste ◽  
Tetracycline Antibiotics ◽  
Tetracycline Resistance Genes

scholarly journals Tetracycline Susceptibility Testing and Resistance Genes in Isolates of Acinetobacter baumannii-Acinetobacter calcoaceticus Complex from a U.S. Military Hospital

2009 ◽  
Vol 53 (6) ◽  
pp. 2693-2695 ◽  
Author(s):  
Kevin S. Akers ◽  
Katrin Mende ◽  
Heather C. Yun ◽  
Duane R. Hospenthal ◽  
Miriam L. Beckius ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Resistance Genes ◽  
Susceptibility Testing ◽  
Tetracycline Resistance ◽  
Acinetobacter Calcoaceticus ◽  
Multidrug Resistant ◽  
Military Hospital ◽  
Tetracycline Resistance Genes ◽  
Testing Method

ABSTRACT Infections with multidrug-resistant Acinetobacter baumannii-Acinetobacter calcoaceticus complex bacteria complicate the care of U.S. military personnel and civilians worldwide. One hundred thirty-three isolates from 89 patients at our facility during 2006 and 2007 were tested by disk diffusion, Etest, and broth microdilution for susceptibility to tetracycline, doxycycline, minocycline, and tigecycline. Minocycline was the most active in vitro, with 90% of the isolates tested susceptible. Susceptibilities varied significantly with the testing method. The acquired tetracycline resistance genes tetA, tetB, and tetA(39) were present in the isolates.

scholarly journals Tetracycline Resistance Genes in Campylobacter jejuni and C. coli Isolated From Poultry Carcasses

2014 ◽  
Vol 7 (8) ◽  
Author(s):  
Bahman Abdi Hachesoo ◽  
Rahem Khoshbakht ◽  
Hassan Sharifi Yazdi ◽  
Mohammad Tabatabaei ◽  
Saeid Hosseinzadeh ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Resistance Genes ◽  
Tetracycline Resistance ◽  
Tetracycline Resistance Genes
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