scholarly journals Complete Sequence of a KPC-Producing IncN Multidrug-Resistant Plasmid from an Epidemic Escherichia coli Sequence Type 131 Strain in China

2014 ◽  
Vol 58 (4) ◽  
pp. 2422-2425 ◽  
Author(s):  
Liang Chen ◽  
Hongyan Hu ◽  
Kalyan D. Chavda ◽  
Shulong Zhao ◽  
Renkun Liu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Multidrug Resistant ◽  
Complete Sequence ◽  
Sequence Type ◽  
Potential Threat ◽  
Content Type ◽  
E Coli ◽  
Rifampin Resistance ◽  
Beijing China

ABSTRACTWe report here the nucleotide sequence of a novelblaKPC-2-harboring incompatibility group N (IncN) plasmid, pECN580, from a multidrug-resistantEscherichia colisequence type 131 (ST131) isolate recovered from Beijing, China. pECN580 harbors β-lactam resistance genesblaKPC-2,blaCTX-M-3, andblaTEM-1; aminoglycoside acetyltransferase geneaac(6′)-Ib-cr; quinolone resistance geneqnrS1; rifampin resistance genearr-3; and trimethoprim resistance genedfrA14. The emergence of ablaKPC-2-harboring multidrug-resistant plasmid in an epidemicE. coliST131 clone poses a significant potential threat in community and hospital settings.

scholarly journals Draft Genomic Sequences of Three Escherichia coli Sequence Type 131 Isolates (H45, H43ii, and H43iii) from Patients in Lagos, Nigeria

2020 ◽  
Vol 9 (17) ◽  
Author(s):  
Yishan Yang ◽  
Christopher H. Sommers ◽  
Eyitayo O. Adenipekun ◽  
Marina Ceruso ◽  
Charlene R. Jackson ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Bloodstream Infections ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Urine Samples ◽  
Genomic Sequences ◽  
Content Type ◽  
E Coli

Escherichia coli sequence type 131 (ST131) has recently emerged as a leading multidrug-resistant pathogen that causes urinary tract and bloodstream infections in humans. Here, we report the draft genomic sequences of three E. coli ST131 isolates, H45, H43ii, and H43iii, from urine samples of patients in Lagos, Nigeria.

scholarly journals Draft Genome Sequence of a Multidrug-Resistant Escherichia coli Sequence Type 1193 Pandemic Clone Isolated from Wastewater in Austria

2021 ◽  
Vol 10 (37) ◽  
Author(s):  
Adriana Cabal ◽  
Nadine Peischl ◽  
Gerhard Rab ◽  
Anna Stöger ◽  
Burkhard Springer ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genome Sequence ◽  
Draft Genome ◽  
Treatment Plant ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Draft Genome Sequence ◽  
Fluoroquinolone Resistance ◽  
Content Type ◽  
E Coli

Extraintestinal Escherichia coli sequence type 1193 (ST1193) is an important source of fluoroquinolone resistance, which has emerged in recent years. We report the first draft genome sequence and annotation of a multidrug-resistant E. coli ST1193 strain obtained from a wastewater treatment plant in Austria.

scholarly journals Rapid Emergence, Subsidence, and Molecular Detection of Escherichia coli Sequence Type 1193-fimH64, a New Disseminated Multidrug-Resistant Commensal and Extraintestinal Pathogen

2019 ◽  
Vol 57 (5) ◽  
Author(s):  
James R. Johnson ◽  
Brian D. Johnston ◽  
Stephen B. Porter ◽  
Connie Clabots ◽  
Tricia L. Bender ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Medical Center ◽  
Virulence Gene ◽  
The United States ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Cross Sectional ◽  
Content Type ◽  
E Coli ◽  
Multiplex Pcr Assay

ABSTRACT Escherichia coli sequence type 1193 (ST1193) is an emerging multidrug-resistant pathogen. We performed longitudinal and cross-sectional surveillance for ST1193 among clinical and fecal E. coli isolates from Minneapolis Veterans Affairs Medical Center (VAMC) patients and their household members, other Minnesota centers, and national VAMCs and compared these ST1193 isolates with archival human and canine ST1193 isolates from Australia (2008). We also developed and extensively validated a novel multiplex PCR assay for ST1193 and its characteristic fimH64 (type 1 fimbrial adhesin) allele. We found that ST1193-H64 (where “H64” refers to a phylogenetic subdivision within ST1193 that is characterized by the fimH64 allele), which was uniformly fluoroquinolone resistant, appeared to emerge in the United States in a geographically staggered fashion beginning around 2011. Its prevalence among clinical and fecal E. coli isolates at the Minneapolis VAMC rose rapidly beginning in 2013, peaked in early 2017, and then plateaued or declined. In comparison with other ST14 complex (STc14) isolates, ST1193-H64 isolates were more extensively multidrug resistant, whereas their virulence genotypes were less extensive but included (uniquely) K1 capsule and fimH64. Pulsed-field gel electrophoresis separated ST1193-H64 isolates from other STc14 isolates and showed genetic commonality between archival Australian versus recent U.S. isolates, fecal versus clinical isolates, and human versus canine isolates. Three main ST1193 pulsotypes differed significantly in resistance profiles and capsular types; emergent pulsotype 2123 was associated with trimethoprim-sulfamethoxazole resistance and K1 (versus K5) capsule. These findings clarify ST1193-H64’s temporal prevalence trends as a fluoroquinolone-resistant pathogen and commensal; document clonal subsets with distinctive geographic, temporal, resistance, and virulence gene associations; and establish a new laboratory tool for rapid and simple detection of ST1193-H64.

scholarly journals Emergence of NDM-5- and MCR-1-Producing Escherichia coli Clones ST648 and ST156 from a Single Muscovy Duck (Cairina moschata)

2016 ◽  
Vol 60 (11) ◽  
pp. 6899-6902 ◽  
Author(s):  
Run-Shi Yang ◽  
Youjun Feng ◽  
Xiao-Yue Lv ◽  
Jia-Hong Duan ◽  
Jing Chen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Food Chain ◽  
Intestinal Flora ◽  
Sequence Type ◽  
Muscovy Duck ◽  
Potential Threat ◽  
Content Type ◽  
E Coli ◽  
Cairina Moschata ◽  
Chain Transmission

ABSTRACTTwoEscherichia coliclones (sequence type 648 [ST648] and ST156) that coproduce NDM-5 and MCR-1 were detected from a single fowl in China. TheblaNDM-5gene was found on the two indistinguishable IncX3 plasmids from the two differentE. coliisolates, whereas themcr-1gene was located on IncHI2 and IncI2 plasmids, respectively, suggesting thatblaNDM-5andmcr-1have spread in avian intestinal flora. Also, the two strains harborblaTEM-1,blaCTX-M-55,fosA3, andaac(6′)-Ib. The multiresistantE. colistrains (especially the epidemic clone ST648) might raise a potential threat to human health via food chain transmission.

scholarly journals OXA-181-Producing Extraintestinal Pathogenic Escherichia coli Sequence Type 410 Isolated from a Dog in Portugal

2020 ◽  
Vol 64 (4) ◽  
Author(s):  
Michael Brilhante ◽  
Juliana Menezes ◽  
Adriana Belas ◽  
Claudia Feudi ◽  
Stefan Schwarz ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Virulence Genes ◽  
Skin Infection ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Whole Genome ◽  
Content Type ◽  
E Coli ◽  
Pathogenic Escherichia Coli ◽  
Tract Infections

ABSTRACT Two multidrug-resistant and carbapenemase-producing Escherichia coli clones of sequence type 410 were isolated from fecal samples of a dog with skin infection on admission to an animal hospital in Portugal and 1 month after discharge. Whole-genome sequencing revealed a 126,409-bp Col156/IncFIA/IncFII multidrug resistance plasmid and a 51,479-bp IncX3 blaOXA-181-containing plasmid. The chromosome and plasmids carried virulence genes characteristic for uropathogenic E. coli, indicating that dogs may carry multidrug-resistant E. coli isolates related to those causing urinary tract infections in humans.

scholarly journals Isolation and Characterization of Escherichia coli Sequence Type 131 and Other Antimicrobial-Resistant Gram-Negative Bacilli from Clinical Stool Samples from Veterans

2016 ◽  
Vol 60 (8) ◽  
pp. 4638-4645 ◽  
Author(s):  
Muhanad Mohamed ◽  
Connie Clabots ◽  
Stephen B. Porter ◽  
Paul Thuras ◽  
James R. Johnson
Keyword(s):  
Escherichia Coli ◽  
Clinical Laboratory ◽  
Medical Center ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Gram Negative ◽  
Content Type ◽  
E Coli ◽  
Isolation And Characterization ◽  
Stool Samples

ABSTRACTEmerging multidrug-resistant (MDR) Gram-negative bacilli (GNB), includingEscherichia colisequence type 131 (ST131) and its resistance-associatedH30 subclone, constitute an ever-growing public health threat. Their reservoirs and transmission pathways are incompletely defined. To assess diarrheal stools as a potential reservoir for ST131-H30 and other MDR GNB, we cultured 100 clinical stool samples from a Veterans Affairs Medical Center clinical laboratory (October to December 2011) for fluoroquinolone- and extended-spectrum cephalosporin (ESC)-resistantE. coliand other GNB, plus totalE. coli. We then characterized selected resistant and susceptibleE. coliisolates by clonal group, phylogenetic group, virulence genotype, and pulsotype and screened all isolates for antimicrobial resistance. Overall, 79 of 100 stool samples yielded GNB (52E. coli; 48 other GNB). Fifteen samples yielded fluoroquinolone-resistantE. coli(10 were ST131, of which 9 wereH30), 6 yielded ESC-resistantE. coli(2 were ST131, both non-H30), and 31 yielded susceptibleE. coli(1 was ST131, non-H30), for 13 total ST131-positive samples. Fourteen non-E. coliGNB were ESC resistant, and three were fluoroquinolone resistant. Regardless of species, almost half (46%) of the fluoroquinolone-resistant and/or ESC-resistant non-E. coliGNB were resistant to at least three drug classes. Fecal ST131 isolates closely resembled reference clinical ST131 isolates according to virulence genotypes and pulsed-field gel electrophoresis (PFGE) profiles. Thus, a substantial minority (30%) of veterans with diarrhea who undergo stool testing excrete antibiotic-resistant GNB, includingE. coliST131. Consequently, diarrhea may pose transmission risks for more than just diarrheal pathogens and may help disseminate clinically relevant ST131 strains and other MDR GNB within hospitals and the community.

scholarly journals Independent Host Factors and Bacterial Genetic Determinants of the Emergence and Dominance of Escherichia coli Sequence Type 131 CTX-M-27 in a Community Pediatric Cohort Study

2019 ◽  
Vol 63 (7) ◽  
Author(s):  
André Birgy ◽  
Corinne Levy ◽  
Marie-Hélène Nicolas-Chanoine ◽  
Aurélie Cointe ◽  
Claire A. Hobson ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Day Care ◽  
Care Center ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Content Type ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Fecal Carriage ◽  
Recent Emergence

ABSTRACT The recent emergence and diffusion in the community of Escherichia coli isolates belonging to the multidrug-resistant and CTX-M-27-producing sequence type 131 (ST131) C1-M27 cluster makes this cluster potentially as epidemic as the worldwide E. coli ST131 subclade C2 composed of multidrug-resistant isolates producing CTX-M-15. Thirty-five extended-spectrum beta-lactamase (ESBL)-producing ST131 isolates were identified in a cohort of 1,885 French children over a 5-year period. They were sequenced to characterize the ST131 E. coli isolates producing CTX-M-27 recently emerging in France. ST131 isolates producing CTX-M-27 (n = 17), and particularly those belonging to the C1-M27 cluster (n = 14), carried many resistance-encoding genes and predominantly an F1:A2:B20 plasmid type. In multivariate analysis, having been hospitalized since birth (odds ratio [OR], 10.9; 95% confidence interval [CI], 2.4 to 48.8; P = 0.002) and being cared for in a day care center (OR, 9.4; 95% CI, 1.5 to 59.0; P = 0.017) were independent risk factors for ST131 CTX-M-27 fecal carriage compared with ESBL-producing non-ST131 isolates. No independent risk factor was found when comparing CTX-M-15 (n = 11)- and CTX-M-1/14 (n = 7)-producing ST131 isolates with ESBL-producing non-ST131 isolates or with non-ESBL-producing isolates. Several factors may contribute to the increase in fecal carriage of CTX-M-27-producing E. coli isolates, namely, resistance to multiple antibiotics, capacity of the CTX-M-27 enzyme to hydrolyze both cefotaxime and ceftazidime, carriage of a peculiar F-type plasmid, and/or capacity to colonize children who have been hospitalized since birth or who attend day care centers.

scholarly journals Plasmids of Diverse Inc Groups Disseminate the Fosfomycin Resistance Gene fosA3 among Escherichia coli Isolates from Pigs, Chickens, and Dairy Cows in Northeast China

2017 ◽  
Vol 61 (9) ◽  
Author(s):  
Xiu-Mei Wang ◽  
Zhimin Dong ◽  
Stefan Schwarz ◽  
Yao Zhu ◽  
Xin Hua ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Dairy Cows ◽  
Resistance Gene ◽  
Northeast China ◽  
Sequence Type ◽  
Content Type ◽  
E Coli ◽  
Conjugative Plasmids ◽  
Fosfomycin Resistance

ABSTRACT Thirty-nine fosfomycin-resistant Escherichia coli isolates carrying fosA3 were obtained from pigs, chickens, dairy cows, and staff in four northeastern provinces of China between June 2015 and April 2016. The fosA3 gene was colocated with bla CTX-M genes on conjugative plasmids of the incompatibility groups IncN (n = 12), IncN-F33:A−:B−(n = 2), IncF33:A−:B−(n = 14), IncF14:A−:B−(n = 2), and IncI1/sequence type 136 (ST136) (n = 9). Four different genetic contexts of fosA3 were detected among the 39 E. coli isolates. Three potential epidemic plasmids circulated among E. coli strains from this region.

scholarly journals Genome Sequencing of an Escherichia coli Sequence Type 617 Strain Isolated from Beach Ghost Shrimp (Callichirus major) from a Heavily Polluted Ecosystem Reveals a Wider Resistome against Heavy Metals and Antibiotics

2019 ◽  
Vol 8 (3) ◽  
Author(s):  
Daniel F. Monte ◽  
Fábio P. Sellera ◽  
Miriam R. Fernandes ◽  
Quézia Moura ◽  
Mariza Landgraf ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Coastal Waters ◽  
Draft Genome ◽  
Genomic Analysis ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Comparative Genomic ◽  
Content Type ◽  
E Coli ◽  
Ghost Shrimp

Here, we present the draft genome sequence of a multidrug-resistant (MDR) Escherichia coli strain belonging to sequence type 617 (ST617), isolated from beach ghost shrimp from polluted coastal waters in Brazil. These data provide valuable information for comparative genomic analysis, related to the dissemination of MDR E. coli in marine ecosystems.

scholarly journals A Novel Trimethoprim Resistance Gene, dfrA36, Characterized from Escherichia coli from Calves

mSphere ◽  
2019 ◽  
Vol 4 (3) ◽  
Author(s):  
Dominik Wüthrich ◽  
Michael Brilhante ◽  
Anna Hausherr ◽  
Jens Becker ◽  
Mireille Meylan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Multidrug Resistant ◽  
Content Type ◽  
Genetic Link ◽  
E Coli ◽  
Global Spread ◽  
Different Sources ◽  
Disinfectant Resistance

The presence of dfrA36 associated with ISCR2 in Escherichia coli from animals, as well as its presence in other E. coli strains from different sources and countries and in Acinetobacter, highlights the global spread of this gene and its potential for further dissemination. The genetic link of ISCR2-dfrA36 with other antibiotic and disinfectant resistance genes showed that multidrug-resistant E. coli may be selected and maintained by the use of either one of several antimicrobials.

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