scholarly journals Coproduction of KPC-2 and IMP-10 in Carbapenem-Resistant Serratia marcescens Isolates from an Outbreak in a Brazilian Teaching Hospital

2015 ◽  
Vol 53 (7) ◽  
pp. 2324-2328 ◽  
Author(s):  
Kesia Esther Silva ◽  
Rodrigo Cayô ◽  
Cecilia Godoy Carvalhaes ◽  
Flávia Patussi Correia Sacchi ◽  
Fernanda Rodrigues-Costa ◽  
...  
Keyword(s):  
Antimicrobial Agent ◽  
Serratia Marcescens ◽  
Teaching Hospital ◽  
Class 1 Integron ◽  
Content Type ◽  
New Class ◽  
Carbapenem Resistant ◽  
Class 1

We describe an outbreak caused by KPC-2- and IMP-10-producingSerratia marcescensisolates in a Brazilian teaching hospital. Tigecycline was the only active antimicrobial agent tested. TheblaIMP-10gene was located in a new class 1 integron, namedIn990, carried by a nonconjugative plasmid, in contrast toblaKPC-2.

scholarly journals OXA-198, an Acquired Carbapenem-Hydrolyzing Class D β-Lactamase from Pseudomonas aeruginosa

2011 ◽  
Vol 55 (10) ◽  
pp. 4828-4833 ◽  
Author(s):  
Farid El Garch ◽  
Pierre Bogaerts ◽  
Carine Bebrone ◽  
Moreno Galleni ◽  
Youri Glupczynski
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Carbapenem Resistance ◽  
Amino Acid Identity ◽  
Ventilator Associated Pneumonia ◽  
Class 1 Integron ◽  
Content Type ◽  
New Class ◽  
Carbapenem Resistant ◽  
Class D ◽  
Class 1

ABSTRACTA carbapenem-resistantPseudomonas aeruginosastrain (PA41437) susceptible to expanded-spectrum cephalosporins was recovered from several consecutive lower-respiratory-tract specimens of a patient who developed a ventilator-associated pneumonia while hospitalized in an intensive care unit. Cloning experiments identified OXA-198, a new class D β-lactamase which was weakly related (less than 45% amino acid identity) to other class D β-lactamases. Expression inEscherichia coliTOP10 and inP. aeruginosaPAO1 led to transformants that were resistant to ticarcillin and showed reduced susceptibility to carbapenems and cefepime. TheblaOXA-198gene was harbored by a class 1 integron carried by a ca. 46-kb nontypeable plasmid. This study describes a novel class D β-lactamase involved in carbapenem resistance inP. aeruginosa.

scholarly journals SMB-1, a Novel Subclass B3 Metallo-β-Lactamase, Associated with ISCR1and a Class 1 Integron, from a Carbapenem-Resistant Serratia marcescens Clinical Isolate

2011 ◽  
Vol 55 (11) ◽  
pp. 5143-5149 ◽  
Author(s):  
Jun-ichi Wachino ◽  
Hiroyuki Yoshida ◽  
Kunikazu Yamane ◽  
Satowa Suzuki ◽  
Mari Matsui ◽  
...  
Keyword(s):  
Serratia Marcescens ◽  
Clinical Isolate ◽  
Apple Orchard ◽  
Resistance Pattern ◽  
Binding Motif ◽  
Class 1 Integron ◽  
Uncultured Bacterium ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACTA carbapenem-resistantSerratia marcescensstrain, 10mdr148, was identified in a Japanese hospital in 2010. The carbapenem resistance of this strain was attributed to the production of a novel metallo-β-lactamase (MBL), named SMB-1 (Serratiametallo-β-lactamase). SMB-1 possessed a zinc binding motif, H(Q)XHXDH (residues 116 to 121), H196, and H263 and was categorized as a member of subclass B3 MBL. SMB-1 has 75% amino acid identity with the most closely related MBL, AMO1, of uncultured bacterium, recently identified through the metagenomic analysis of apple orchard soil. The introduction ofblaSMB-1intoEscherichia coliconferred resistance to a variety of β-lactam antibiotics, penicillins, cephalosporins, and carbapenems, but not aztreonam, a resistance pattern consistent with those of other MBLs. SMB-1 demonstrated highkcatvalues of >500 s−1for carbapenems, resulting in the highest hydrolyzing efficiency (kcat/Km) among the agents tested. The hydrolyzing activity of SMB-1 was well inhibited by chelating agents. TheblaSMB-1gene was located on the chromosome ofS. marcescensstrain 10mdr148 and at the 3′ end of the ISCR1element in complex with a typical class 1 integron carryingaac(6′)-IbandcatB3gene cassettes. Downstream ofblaSMB-1, the second copy of the 3′conserved segment and ISCR1were found. To our knowledge, this is the first subclass B3 MBL gene associated with an ISCR1element identified in anEnterobacteriaceaeclinical isolate. A variety of antibiotic resistance genes embedded with ISCR1have been widely spread amongEnterobacteriaceaeclinical isolates, thus the further dissemination ofblaSMB-1mediated by ISCR1transposition activity may become a future concern.

scholarly journals A Multispecies Cluster of VIM-1 Carbapenemase-Producing Enterobacterales Linked by a Novel, Highly Conjugative, and Broad-Host-Range IncA Plasmid Forebodes the Reemergence of VIM-1

2020 ◽  
Vol 64 (4) ◽  
Author(s):  
Gabriele Arcari ◽  
Federica Maria Di Lella ◽  
Giulia Bibbolino ◽  
Fabio Mengoni ◽  
Marzia Beccaccioli ◽  
...  
Keyword(s):  
Host Range ◽  
Bacterial Species ◽  
Klebsiella Oxytoca ◽  
Gene Cassette ◽  
University Hospital ◽  
Broad Host Range ◽  
Class 1 Integron ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACT In this study, we investigated VIM-1-producing Escherichia coli, Klebsiella oxytoca, Klebsiella pneumoniae, Citrobacter freundii, and Enterobacter cloacae strains, isolated in 2019 during a period of active surveillance of carbapenem-resistant Enterobacterales in a large university hospital in Italy. VIM-1-producing strains colonized the gut of patients, with up to three different VIM-1-positive bacterial species isolated from a single rectal swab, but also caused bloodstream infection in one colonized patient. In the multispecies cluster, blaVIM-1 was identified in a 5-gene cassette class 1 integron, associated with several genetic determinants, including the blaSHV-12, qnrS1, and mph(A) genes, located on a highly conjugative and broad-host-range IncA plasmid. The characteristics and origin of this IncA plasmid were studied.

scholarly journals Infrequent Finding of Metallo-β-Lactamase VIM-2 in Carbapenem-Resistant Pseudomonas aeruginosa Strains from Croatia

2012 ◽  
Vol 56 (5) ◽  
pp. 2746-2749 ◽  
Author(s):  
Sanda Sardelic ◽  
Branka Bedenic ◽  
Céline Colinon-Dupuich ◽  
Stjepan Orhanovic ◽  
Zrinka Bosnjak ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Coastal Region ◽  
Large Hospital ◽  
Class 1 Integron ◽  
Content Type ◽  
Variable Part ◽  
Carbapenem Resistant ◽  
Class 1 ◽  
Sequence Types

ABSTRACTOne hundred sixty-nine nonreplicate imipenem-resistantPseudomonas aeruginosastrains isolated in a large hospital on the coastal region of Croatia were studied. The most active antibiotics were colistin and amikacin. Most of the isolates were multiresistant. The most prevalent serotype was O12, followed by O11. Six strains carried theblaVIM-2gene located in a novel class 1 integron composed in its variable part of theblaVIM-2-blaoxa-10-ΔqacF-aacA4genes. Metallo-β-lactamase-producing strains belonged to sequence types ST235 and ST111.

scholarly journals First Report ofblaIMP-14on a Plasmid Harboring Multiple Drug Resistance Genes in Escherichia coli Sequence Type 131

2016 ◽  
Vol 60 (8) ◽  
pp. 5068-5071 ◽  
Author(s):  
Nicole Stoesser ◽  
Anna E. Sheppard ◽  
Gisele Peirano ◽  
Robert P. Sebra ◽  
Tarah Lynch ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Multiple Drug Resistance ◽  
Carbapenem Resistance ◽  
Sequence Type ◽  
Multiple Drug ◽  
Class 1 Integron ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACTTheblaIMP-14carbapenem resistance gene has largely previously been observed inPseudomonas aeruginosaandAcinetobacterspp. As part of global surveillance and sequencing of carbapenem-resistantEscherichia coli, we identified a sequence type 131 strain harboringblaIMP-14within a class 1 integron, itself nested within an ∼54-kb multidrug resistance region on an epidemic IncA/C2plasmid. The emergence ofblaIMP-14in this context in the ST131 lineage is of potential clinical concern.

scholarly journals First Report ofblaIMP-8in Raoultella planticola

2013 ◽  
Vol 58 (1) ◽  
pp. 593-595 ◽  
Author(s):  
Sung-Pin Tseng ◽  
Jann-Tay Wang ◽  
Chih-Yuan Liang ◽  
Pei-Shan Lee ◽  
Yee-Chun Chen ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Protein Loss ◽  
Class 1 Integron ◽  
Raoultella Planticola ◽  
Content Type ◽  
First Report ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACTTwo carbapenem-resistantRaoultella planticolaclinical isolates were isolated from patients with pneumonia and Port-A catheter-related bacteremia, respectively, in Taiwan. These isolates remained susceptible to fluoroquinolone, aminoglycoside, and colistin. Though the two isolates had the same antibiogram, plasmidic carbapenemaseblaIMP-8, class 1 integron cassette (dfrA12-orfF-aadA2), andqnrB2, they had different pulsed-field gel electrophoresis patterns, plasmid sizes, and outer membrane protein loss profiles. To our knowledge, this is the first report ofblaIMP-8found inR. planticola. Interestingly,blaIMP-8is the most common carbapenemase found inKlebsiella pneumoniaein Taiwan. In the literature, carbapenemase genes inR. planticolain each country were also found in carbapenem-resistantEnterobacteriaceaein the same country.

scholarly journals Clinical Carbapenem-Resistant Acinetobacter baylyi Strain CoharboringblaSIM-1andblaOXA-23from China

2011 ◽  
Vol 55 (11) ◽  
pp. 5347-5349 ◽  
Author(s):  
Zhihui Zhou ◽  
Xiaoxing Du ◽  
Li Wang ◽  
Qing Yang ◽  
Yiqi Fu ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Acinetobacter Baumannii ◽  
Clonal Complex ◽  
Gene Cassette ◽  
Large Plasmid ◽  
Acinetobacter Baylyi ◽  
Class 1 Integron ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACTblaSIM-1andblaOXA-23were codetected in clinical carbapenem-resistantAcinetobacter baylyistrain NB09A30. Both of carbapenemase genes were located on a large plasmid (ca. 360 kb).blaSIM-1was found as a gene cassette inserted into a class 1 integron identical to that determined inAcinetobactersp. isolates from South Korea. The genetic structure ofblaOXA-23in NB09A30 was different from that in the prevalentAcinetobacter baumanniiof clonal complex 92 (CC92) from the same hospital.

scholarly journals Complete Sequence of pOZ176, a 500-Kilobase IncP-2 Plasmid Encoding IMP-9-Mediated Carbapenem Resistance, from Outbreak Isolate Pseudomonas aeruginosa 96

2013 ◽  
Vol 57 (8) ◽  
pp. 3775-3782 ◽  
Author(s):  
Jianhui Xiong ◽  
David C. Alexander ◽  
Jennifer H. Ma ◽  
Maxime Déraspe ◽  
Donald E. Low ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Carbapenem Resistance ◽  
Complete Sequence ◽  
Open Reading Frames ◽  
Content Type ◽  
Conserved Sequence ◽  
Carbapenem Resistant ◽  
Class 1 ◽  
Usage Analysis ◽  
Type 3

ABSTRACTPseudomonas aeruginosa96 (PA96) was isolated during a multicenter surveillance study in Guangzhou, China, in 2000. Whole-genome sequencing of this outbreak strain facilitated analysis of its IncP-2 carbapenem-resistant plasmid, pOZ176. The plasmid had a length of 500,839 bp and an average percent G+C content of 57%. Of the 618 predicted open reading frames, 65% encode hypothetical proteins. The pOZ176 backbone is not closely related to any plasmids thus far sequenced, but some similarity to pQBR103 ofPseudomonas fluorescensSBW25 was observed. Two multiresistant class 1 integrons and several insertion sequences were identified. TheblaIMP-9-carrying integron containedaacA4→blaIMP-9→aacA4, flanked upstream by Tn21 tnpMRAand downstream by a completetnioperon of Tn402and amermodule, named Tn6016. The second integron carriedaacA4→catB8a→blaOXA-10and was flanked by Tn1403-liketnpRAand asul1-type 3′ conserved sequence (3′-CS), named Tn6217. Other features include three resistance genes similar to those of Tn5, a tellurite resistance operon, and twopiloperons. The replication and maintenance systems exhibit similarity to a genomic island ofRalstonia solanacearumGM1000. Codon usage analysis suggests the recent acquisition ofblaIMP-9. The origins of the integrons on pOZ176 indicated separate horizontal gene transfer events driven by antibiotic selection. The novel mosaic structure of pOZ176 suggests that it is derived from environmental bacteria.

scholarly journals Molecular Characterization ofblaNDM-1in a Sequence Type 235 Pseudomonas aeruginosa Isolate from France

2013 ◽  
Vol 57 (7) ◽  
pp. 3408-3411 ◽  
Author(s):  
Frédéric Janvier ◽  
Katy Jeannot ◽  
Sophie Tessé ◽  
Marjorie Robert-Nicoud ◽  
Hervé Delacour ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Insertion Sequence ◽  
Variable Region ◽  
Sequence Type ◽  
Common Region ◽  
Class 1 Integron ◽  
Genetic Studies ◽  
Content Type ◽  
Class 1 ◽  
Previous Hospitalization

ABSTRACTAn NDM-1 carbapenemase-producingPseudomonas aeruginosaisolate was recovered from a patient hospitalized in France after a previous hospitalization in Serbia. Genetic studies revealed that theblaNDM-1gene was surrounded by insertion sequence ISAba125and a truncated bleomycin resistance gene. ThisblaNDM-1region was a part of the variable region of a new complex class 1 integron bearing IS common region 1 (ISCR1). The presence of ISPa7upstream of this integron suggests insertion in a chromosomally located Tn402-like structure.

scholarly journals Diversity and Evolution of AbaR Genomic Resistance Islands in Acinetobacter baumannii Strains of European Clone I

2011 ◽  
Vol 55 (7) ◽  
pp. 3201-3206 ◽  
Author(s):  
Lenka Krizova ◽  
Lenie Dijkshoorn ◽  
Alexandr Nemec
Keyword(s):  
Acinetobacter Baumannii ◽  
Selective Advantage ◽  
Multidrug Resistant ◽  
Original Form ◽  
Class 1 Integron ◽  
Content Type ◽  
Atpase Gene ◽  
Class 1 ◽  
Major Clone ◽  
Derivatives Of

ABSTRACTTo assess the diversity of AbaR genomic resistance islands inAcinetobacter baumanniiEuropean clone I (MLST clonal complex 1), we investigated 26 multidrug-resistant strains of this major clone isolated from hospitals in 21 cities of 10 European countries between 1984 and 2005. Each strain harbored an AbaR structure integrated at the same position in the chromosomal ATPase gene. AbaR3, including four subtypes based on variations in class 1 integron cassettes, and AbaR10 were found in 15 and 2 strains, respectively, whereas a new, unique AbaR variant was discovered in each of the other 9 strains. These new variants, designated AbaR11 to AbaR19 (19.8 kb to 57.5 kb), seem to be truncated derivatives of AbaR3, likely resulting from the deletions of its internal parts mediated by either IS26elements (AbaR12 to AbaR19) or homologous recombination (AbaR11). AbaR3 was detected in all 10 strains isolated in 1984 to 1991, while AbaR11 to AbaR19 were carried only by strains isolated since 1997. Our results and those from previous publications suggest that AbaR3 is the original form of AbaR in European clone I, which may have provided strains of the lineage with a selective advantage facilitating their spread in European hospitals in the 1980s or before.

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