scholarly journals Sensitive Bioassay for Determination of Fluconazole Concentrations in Plasma Using a Candida albicans Mutant Hypersusceptible to Azoles

2001 ◽  
Vol 45 (3) ◽  
pp. 696-700 ◽  
Author(s):  
Oscar Marchetti ◽  
Paul A. Majcherczyk ◽  
Michel P. Glauser ◽  
Jacques Bille ◽  
Philippe Moreillon ◽  
...  
Keyword(s):  
High Performance ◽  
Correlation Coefficients ◽  
Experimental Treatment ◽  
Cost Effective ◽  
Clinical Settings ◽  
Multidrug Efflux ◽  
Analytical Method Validation ◽  
Accuracy And Precision ◽  
Cost Effective Method ◽  
New Bioassay

ABSTRACT The antifungal agent fluconazole (FLC) is widely used in clinical practice. Monitoring FLC levels is useful in complicated clinical settings and in experimental infection models. A bioassay usingCandida pseudotropicalis, a simple and cost-effective method, is validated only for FLC levels ranging from 5 to 40 mg/liter. An extension of the analytical range is needed to cover most yeast MICs. A new bioassay in RPMI agar containing methylene blue was developed using C. albicans DSY1024, a mutant rendered hypersusceptible to FLC constructed by the deletion of the multidrug efflux transporter genes CDR1, CDR2, CaMDR1, andFLU1. Reproducible standard curves were obtained with FLC concentrations in plasma ranging from 1 to 100 mg/liter (quadratic regression coefficient > 0.997). The absolute sensitivity was 0.026 μg of FLC. The method was internally validated according to current guidelines for analytical method validation. Both accuracy and precision lied in the required ±15% range. FLC levels measured by bioassay and by high-performance liquid chromatography (HPLC) performed with 62 plasma samples from humans and rats showed a strong correlation (coefficients, 0.979 and 0.995, respectively; percent deviations of bioassay from HPLC values, 0.44% ± 15.31% and 2.66% ± 7.54%, respectively). In summary, this newly developed bioassay is sensitive, simple, rapid, and inexpensive. It allows nonspecialized laboratories to determine FLC levels in plasma to within the clinically relevant concentration range and represents a useful tool for experimental treatment models.

scholarly journals Flavonoid Quantification in Onion by Spectrophotometric and High Performance Liquid Chromatography Analysis

HortScience ◽  
2002 ◽  
Vol 37 (4) ◽  
pp. 682-685 ◽  
Author(s):  
Kevin A. Lombard ◽  
Emmanuel Geoffriau ◽  
Ellen Peffley
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Hplc Analysis ◽  
Correlation Coefficients ◽  
Cost Effective ◽  
Chromatography Analysis ◽  
Cost Effective Method ◽  
Performance Liquid Chromatography Analysis

Direct spectrophotometric determination of quercetin content in onions (Allium cepa L.) was investigated as a possible alternative to high-performance liquid chromatography (HPLC) analysis. Quercetin content in five onion varieties was monitored at 362 nm and quantified using simple spectrophotometric and HPLC methods. HPLC revealed that 3,4'-Qdg and 4'-Qmg comprised up to 93% of total flavonol content detected in the studied varieties. These major quercetin conjugates combined (3,4'-Qdg + 4'-Qmg) and total flavonol conjugates quantified by HPLC correlated closely with spectrophotometer values. Correlation coefficients were 0.96 (P < 0.0001) for 3,4'-Qdg + 4'-Qmg and 0.97 (P < 0.0001) for total flavonol conjugates in onion. Simple spectrophotometric procedure proved to be a valid, efficient, and cost-effective method for the quantification of total quercetin in onion. Chemical names used: quercetin-3,4'-O-diglucoside (3,4'-Qdg); quercetin-4'-O-glucoside (4'-Qmg).

scholarly journals Rapid Detection and Quantification of Patulin and Citrinin Contamination in Fruits

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4545
Author(s):  
Sudharsan Sadhasivam ◽  
Omer Barda ◽  
Varda Zakin ◽  
Ram Reifen ◽  
Edward Sionov
Keyword(s):  
High Performance ◽  
Cost Effective ◽  
Hplc Method ◽  
Pear Fruit ◽  
Cost Effective Method ◽  
Fruit Samples ◽  
Pome Fruits ◽  
Reliable Methods ◽  
By Products ◽  
Detection And Quantification

Patulin (PAT) and citrinin (CTN) are the most common mycotoxins produced by Penicillium and Aspergillus species and are often associated with fruits and fruit by-products. Hence, simple and reliable methods for monitoring these toxins in foodstuffs are required for regular quality assessment. In this study, we aimed to establish a cost-effective method for detection and quantification of PAT and CTN in pome fruits, such as apples and pears, using high-performance liquid chromatography (HPLC) coupled with spectroscopic detectors without the need for any clean-up steps. The method showed good performance in the analysis of these mycotoxins in apple and pear fruit samples with recovery ranges of 55–97% for PAT and 84–101% for CTN, respectively. The limits of detection (LOD) of PAT and CTN in fruits were 0.006 µg/g and 0.001 µg/g, while their limits of quantification (LOQ) were 0.018 µg/g and 0.003 µg/g, respectively. The present findings indicate that the newly developed HPLC method provides rapid and accurate detection of PAT and CTN in fruits.

scholarly journals Extractive Spectrophotometric Methods for Determination of Zolmitriptan in Tablets

2007 ◽  
Vol 90 (5) ◽  
pp. 1237-1241 ◽  
Author(s):  
Zeynep Aydogmus ◽  
Ipek Inanli
Keyword(s):  
High Performance ◽  
Correlation Coefficients ◽  
Bromothymol Blue ◽  
Chromatography Method ◽  
Statistical Comparison ◽  
Spectrophotometric Methods ◽  
Accuracy And Precision ◽  
Liquid Chromatography Method ◽  
Comparison Of The Results

Abstract Two simple and sensitive extractive spectrophotometric methods have been developed for determination of zolmitriptan (ZTP) in tablets. These methods are based on the formation of yellow ion-pair complexes between ZTP and tropaeolin OO (TPOO) and bromothymol blue (BTB) in citratephosphate buffer of pH 4.0 and 6.0, respectively. The formed complexes were extracted with dichloromethane and measured at 411.5 and 410 nm for TPOO and BTB, respectively. The best conditions of the reactions were studied and optimized. Beer's law was obeyed in the concentration ranges of 220 and 1.517 g/mL with molar absorptivities of 1.42 104 and 1.60 104 L/mol/cm for the TPOO and BTB methods, respectively. Correlation coefficients were 0.9998 and 0.9999 for TPOO and BTB methods, respectively. Limits of detection of the TPOO and BTB methods were 0.341 and 0.344 g/mL, respectively, and the limits of quantitation were 1.034 and 1.051 g/mL, respectively. Sandell's sensitivity and stability constant were also calculated. The proposed methods have been applied successfully for the analysis of the drug in its dosage forms. No interference was observed from excipients present in tablets. Statistical comparison of the results with those obtained by a high-performance liquid chromatography method showed excellent agreement and indicated no significant differences in accuracy and precision.

scholarly journals Determination of Pesticide Residue in Brinjal Sample Using HPTLC and Developing a Cost-Effective Method Alternative to HPLC

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Muzammil Hussain ◽  
Kiran Aftab ◽  
Munawar Iqbal ◽  
Shafaqat Ali ◽  
Muhammad Rizwan ◽  
...  
Keyword(s):  
High Performance ◽  
Hplc Analysis ◽  
Organophosphorus Pesticide ◽  
Analytical Techniques ◽  
Cost Effective ◽  
Local Market ◽  
Standard Samples ◽  
Residual Concentration ◽  
Food And Agriculture ◽  
Cost Effective Method

Two analytical techniques HPLC (high performance liquid chromatography) and HPTLC (high performance thin layer chromatography) were validated to reveal the quality and quantity of pesticide residues (organophosphorus, organochlorine, and pyrethroids) in brinjal samples collected from a local market of Faisalabad. The HPTLC methods showed linear behavior for standard samples and residue was in the range of 1–130 ng. The organochlorine (α-endosulfan) contaminates the samples at 4, 5, 9, and 10 weeks, and detected quantity was less than MRL (minimum residue level) of the FAO (Food and Agriculture Organization of United Nations), i.e., 0.5 mg·kg−1. The organophosphorus pesticide (chlorpyriphos, methamidophos, monocrotophos, dichlorvos, carbosulfan, profenophos, and dimethoate) residue contaminated the samples and violated the MRL limit. Pyrethroids (deltamethrin, β-cyhalothrin, and cypermethrin) were present at appreciable levels, in samples of 1, 3, 4, 6, 8, and 9 weeks. The concentration of β-cyhalothrin (0.25 mg·kg−1) and cypermethrin (0.205 mg·kg−1) was significantly higher than that of all detected pesticides. The carbosulfan and deltamethrin contaminated all 10-week samples. The HPLC analysis of samples was carried out to confirm the efficiency of HPTLC as cost-effective method. The concentration of α-endosulfan, chlorpyriphos, dimethoate, monocrotophos, profenophos, deltamethrin, and cypermethrin in brinjal samples through the HPTLC method showed similar residual concentration with HPLC analysis.

Water quality monitoring by Thamnotoxkit F™ including cyanobacterial blooms

2000 ◽  
Vol 42 (1-2) ◽  
pp. 381-385 ◽  
Author(s):  
A. Kozma Törökné ◽  
E. László ◽  
I. Chorus ◽  
J. Fastner ◽  
R. Heinze ◽  
...  
Keyword(s):  
High Performance ◽  
Cost Effective ◽  
Water Quality Monitoring ◽  
Cyanobacterial Blooms ◽  
Mouse Bioassay ◽  
Test Mouse ◽  
Cost Effective Method ◽  
Freeze Dried ◽  
Test Kit ◽  
Cyanobacterial Toxicity

The Thamnotoxkit F™ was evaluated for detecting cyanobacterial toxins as they may be hazardous to human health if they reach drinking water networks or if people are exposed through recreational activity. This test kit is a 24-hour bioassay using larvae of the freshwater anostracan crustacean Thamnocephalus platyurus hatched from cysts. Nine freeze-dried Microcystis aeruginosa samples from freshwaters of Hungary, Germany and Brazil were tested with the Thamnotox test, rat hepatocyte test, mouse test and analysed for microcystins by high performance liquid chromatography (HPLC). It can be concluded that the Thamnotox test is an alternative simple, cost-effective method that may replace the mouse bioassay used previously for determination of cyanobacterial toxicity.

scholarly journals An Automated and High-Throughput Immunoaffinity Magnetic Bead-Based Sample Clean-Up Platform for the Determination of Aflatoxins in Grains and Oils Using UPLC-FLD

Toxins ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 583 ◽  
Author(s):  
Zhihong Xuan ◽  
Jin Ye ◽  
Bing Zhang ◽  
Li Li ◽  
Yu Wu ◽  
...  
Keyword(s):  
High Throughput ◽  
High Performance ◽  
Cost Effective ◽  
Magnetic Bead ◽  
The Novel ◽  
Analysis Method ◽  
Automated Method ◽  
Accuracy And Precision ◽  
Optimized Conditions

Sample clean-up remains the most time-consuming and error-prone step in the whole analytical procedure for aflatoxins (AFTs) analysis. Herein, an automated and high-throughput sample clean-up platform was developed with a disposable, cost-effective immunoaffinity magnetic bead-based kit. Under optimized conditions, the automated method takes less than 30 min to simultaneously purify 20 samples without requiring any centrifugation or filtering steps. When coupled to ultra-high performance liquid chromatography with fluorescence detection, this new analysis method displays excellent accuracy and precision as well as outstanding efficiency. Furthermore, an interlaboratory study was performed in six laboratories to validate the novel protocol. Mean recovery, repeatability, reproducibility, and Horwitz ratio values were within 91.9%–107.4%, 2.5%–7.4%, 2.7%–10.6%, and 0.26%–0.90, respectively. Results demonstrate that the developed sample clean-up platform is a reliable alternative to most widely adopted clean-up procedures for AFTs in cereals and oils.

High performance work practices and competitive advantage in the Irish hospitality sector

2007 ◽  
Vol 19 (3) ◽  
pp. 201-210 ◽  
Author(s):  
Pauline Connolly ◽  
Geraldine McGing
Keyword(s):  
High Performance ◽  
Management Practices ◽  
Cost Effective ◽  
Work Practices ◽  
Performance Practices ◽  
Content Type ◽  
High Performing ◽  
Competitive Edge ◽  
Cost Effective Method ◽  
High Performance Work Practices

PurposeFailte Ireland argues that high performance work practices will provide the Irish tourist industry with the necessary competitive edge. The main focus of this study is to explore the extent of these practices in the Irish hospitality industry with particular emphasis on the practices of staff empowerment and participation.Design/methodology/approachA questionnaire was chosen as the most appropriate means of obtaining information from managers because respondents could be targeted in a cost effective method. A total of 71 questionnaires were mailed to managers in all three, four and five‐star hotels in the Dublin and greater Dublin area.FindingsThe data show that Dublin‐based hotels display some of the human resource practices associated with high performing work practices. There are, however, very low levels of employee participation, which many authors argue are the cornerstone of high performance practices.Practical implicationsThe Irish hotel industry is undergoing fundamental change, not least of which is the increasingly demanding and discerning clients. Customers are demanding quality products and services, speed and reliability of service, price competitiveness and innovation. If Irish hotels are to survive and remain competitive, the issue of staff representation, participation and the channels of communication will have to be addressed. Recognising, utilizing and developing their human resources may be the most significant challenge that management faces, and it is posited that those organisations that excel at this will be the industry leaders.Originality/valueThe paper offers insights into the realities of management practices in hotels in an Irish setting.

Sodium modified molybdenum sulfide via molten salt electrolysis as an anode material for high performance sodium-ion batteries

2016 ◽  
Vol 18 (4) ◽  
pp. 3204-3213 ◽  
Author(s):  
Shuai Wang ◽  
Jiguo Tu ◽  
Yan Yuan ◽  
Rui Ma ◽  
Shuqiang Jiao
Keyword(s):  
Molten Salt ◽  
Anode Material ◽  
High Performance ◽  
Cost Effective ◽  
Molybdenum Sulfide ◽  
Sodium Ion ◽  
Sodium Ion Batteries ◽  
Molten Salt Electrolysis ◽  
Cost Effective Method

The paper reports a facile and cost effective method for fabricating sodium molybdenum sulfide nanoparticles through using MoS2sheets as the precursor by sodium-modification.

scholarly journals Cost Effective, Efficient and Stability indicating analytical method validation for Ranolazine related by Reverse Phase High Performance Liquid Chromatography in drug substances

2020 ◽  
Vol 10 (6-s) ◽  
pp. 45-54
Author(s):  
Pushpendra Sharma ◽  
KLV Satyanarayana ◽  
G. Sri Hari
Keyword(s):  
Method Validation ◽  
Mobile Phase ◽  
High Performance ◽  
Cost Effective ◽  
Hplc Method ◽  
Pharmaceutical Dosage Forms ◽  
Column Temperature ◽  
Analytical Method Validation ◽  
Drug Substances ◽  
Ich Guidelines

A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of Ranolazine in drug substances. Isocratic elution at a flow rate of 1.4ml/min was employed on Hypersil BDS C18, 150 x 4.6 mm, 5µm or Equivalent at 40°C column temperature. The mobile phase consisted of Mobile phase-A: Mobile phase-B (55:45) (Disodium hydrogen orthophosphate buffer with pH 7.0 and Acetonitrile). The UV detection wavelength was at 205 nm. Linearity was observed in concentration range of 0.07-0.82 ppm for ranolazine impurity-I and concentration range of 0.07-0.78 ppm for ranolazine impurity-II. The retention time for Ranolazine was 7.6 min. The method was validated for validation parameter like specificity, force degradation, linearity, accuracy, precision and robustness as per the ICH guidelines. The proposed method can be successfully applied for the estimation of Ranolazine in pharmaceutical dosage forms. Keywords: Ranolazine, Method Validation, Drug Substances, HPLC

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