scholarly journals Analysis of the Lactobacillus Metabolic Pathway

2010 ◽  
Vol 76 (21) ◽  
pp. 7299-7301 ◽  
Author(s):  
Masahiro Kuratsu ◽  
Yoshimitsu Hamano ◽  
Tohru Dairi
Keyword(s):  
Amino Acids ◽  
Folic Acid ◽  
Metabolic Pathway ◽  
Alternative Pathway ◽  
Lactobacillus Fermentum ◽  
Acid Biosynthesis

ABSTRACT We performed analyses of the phenotypic and genotypic relationships focusing on biosyntheses of amino acids, purine/pyrimidines, and cofactors in three Lactobacillus strains. We found that Lactobacillus fermentum IFO 3956 perhaps synthesized para-aminobenzoate (PABA), an intermediate of folic acid biosynthesis, by an alternative pathway.

scholarly journals Incorporation of alternative amino acids into cyanophycin by different cyanophycin synthetases heterologously expressed in Corynebacterium glutamicum

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ramona Wördemann ◽  
Lars Wiefel ◽  
Volker F. Wendisch ◽  
Alexander Steinbüchel
Keyword(s):  
Amino Acids ◽  
Dry Weight ◽  
Growth Conditions ◽  
Amino Acid Biosynthesis ◽  
Water Soluble ◽  
Lysine Content ◽  
Polyaspartic Acid ◽  
Acid Biosynthesis ◽  
Cyanophycin Synthetase ◽  
Potential Precursor

AbstractCyanophycin (multi-l-arginyl-poly-l-aspartic acid; also known as cyanophycin grana peptide [CGP]) is a biopolymer that could be used in various fields, for example, as a potential precursor for the synthesis of polyaspartic acid or for the production of CGP-derived dipeptides. To extend the applications of this polymer, it is therefore of interest to synthesize CGP with different compositions. A recent re-evaluation of the CGP synthesis in C. glutamicum has shown that C. glutamicum is a potentially interesting microorganism for CGP synthesis with a high content of alternative amino acids. This study shows that the amount of alternative amino acids can be increased by using mutants of C. glutamicum with altered amino acid biosynthesis. With the DM1729 mutant, the lysine content in the polymer could be increased up to 33.5 mol%. Furthermore, an ornithine content of up to 12.6 mol% was achieved with ORN2(Pgdh4). How much water-soluble or insoluble CGP is synthesized is strongly related to the used cyanophycin synthetase. CphADh synthesizes soluble CGP exclusively. However, soluble CGP could also be isolated from cells expressing CphA6308Δ1 or CphA6308Δ1_C595S in addition to insoluble CGP in all examined strains. The point mutation in CphA6308Δ1_C595S partially resulted in a higher lysine content. In addition, the CGP content could be increased to 36% of the cell dry weight under optimizing growth conditions in C. glutamicum ATCC13032. All known alternative major amino acids for CGP synthesis (lysine, ornithine, citrulline, and glutamic acid) could be incorporated into CGP in C. glutamicum.

Stability of Folic Acid in 25% Dextrose, 3.5% Amino Acids, and Multivitamin Solution

1984 ◽  
Vol 8 (4) ◽  
pp. 421-426 ◽  
Author(s):  
Nancy Louie ◽  
Douglass J. Stennett
Keyword(s):  
Amino Acids ◽  
Folic Acid

Effects of an Electric Field of Sinusoidal Waves on the Amino Acid Biosynthesis by Azotobacter

1980 ◽  
Vol 35 (3-4) ◽  
pp. 258-261
Author(s):  
A. Martin Gonzalez ◽  
M. T. Izquierdo
Keyword(s):  
Amino Acids ◽  
Nitrogen Fixation ◽  
Amino Acid ◽  
Electric Field ◽  
Electric Fields ◽  
Culture Medium ◽  
Azotobacter Vinelandii ◽  
Amino Acid Biosynthesis ◽  
Medium Composition ◽  
Acid Biosynthesis

Abstract Electric Field Electric fields of sinusoidal waves have been applied in cultures of Azotobacter vinelandii, with potentials between 0 V and 10 V, intensities from 0 mA to 16 mA and frequencies between 5 Hz and 200 KHz. The influence of the electric field of sinusoidal waves on the nitrogen fixation on the post­ culture medium composition has a maximum at 5 V, 8 mA and 20 Hz. The rate of synthesis of specific amino acids by Azotobacter depends on the frequency and potential of the electric field applied. The concentration of each amino acid present in the post-culture medium is increased according to the electric field employed and the amino acid biosynthesis in culture medium is activated during the first days of incubation.

Fatty acid recycling in adipocytes: a role for glyceroneogenesis and phosphoenolpyruvate carboxykinase

2003 ◽  
Vol 31 (6) ◽  
pp. 1125-1129 ◽  
Author(s):  
C. Forest ◽  
J. Tordjman ◽  
M. Glorian ◽  
E. Duplus ◽  
G. Chauvet ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Amino Acids ◽  
Adipose Tissue ◽  
Fatty Acid ◽  
White Adipose Tissue ◽  
Metabolic Pathway ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Important Pathway ◽  
Low Activity

FA (fatty acid) recycling in adipose tissue appears to be an important pathway for regulating FA release into the blood during fasting. Re-esterification requires G3P (glycerol 3-phosphate), which cannot be synthesized from glucose because glycolysis is much reduced under such circumstances. In addition, G3P can scarcely originate from glycerol since glycerol kinase has a very low activity in white adipose tissue. It was shown about 35 years ago that a metabolic pathway named glyceroneogenesis, which allows G3P synthesis from non-carbohydrate precursors like pyruvate, lactate or amino acids, is activated during fasting. The major enzyme in this pathway was shown to be PEPCK-C [cytosolic phosphoenolpyruvate carboxykinase (GTP); EC 4.1.1.32]. The present review analyses the mechanisms by which a series of hormones and nutrients affect PEPCK-C gene transcription and glyceroneogenesis and describes evidence for dysregulation of this pathway in type 2 diabetes.

scholarly journals Regulation of indole-3-acetic acid biosynthesis by branched-chain amino acids inEnterobacter cloacaeUW5

2015 ◽  
Vol 362 (18) ◽  
pp. fnv153 ◽  
Author(s):  
Cassandra V. Parsons ◽  
Danielle M. M. Harris ◽  
Cheryl L. Patten
Keyword(s):  
Amino Acids ◽  
Acetic Acid ◽  
Branched Chain Amino Acids ◽  
Acid Biosynthesis ◽  
Branched Chain ◽  
Indole 3 Acetic Acid

scholarly journals Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

10.3791/3583 ◽  
2012 ◽  
Author(s):  
Le You ◽  
Lawrence Page ◽  
Xueyang Feng ◽  
Bert Berla ◽  
Himadri B. Pakrasi ◽  
...  
Keyword(s):  
Amino Acids ◽  
Metabolic Pathway

The excystation of Entamoeba histolytica without bacteria in microcultures

Parasitology ◽  
1950 ◽  
Vol 40 (3-4) ◽  
pp. 338-342 ◽  
Author(s):  
Charles W. Rees ◽  
Lucy V. Reardon ◽  
Ida Louise Bartgis
Keyword(s):  
Carbon Dioxide ◽  
Amino Acids ◽  
Folic Acid ◽  
Vitamin C ◽  
Trypanosoma Cruzi ◽  
Organic Compounds ◽  
Entamoeba Histolytica ◽  
Plasmodium Knowlesi ◽  
Sodium Potassium ◽  
Potassium Magnesium

1. Formulae developed by Anfinsen et al. (1946) for a medium used in the cultivation of Plasmodium knowlesi were used for media in which excystation of Entamoeba histolytica without bacteria was investigated.2. The following media were used: (i) an inorganic fluid containing carbon dioxide and chlorides, phosphates, and bicarbonates, of sodium, potassium, magnesium, and calcium; (ii) an inorganic fluid of chlorides and phosphates of the above metals without bicarbonates; (iii) the same respective fluids plus glucose, and (iv) the same plus enrichments with B vitamins, cocarboxylase, vitamin C, purines, pyrimidines, folic acid, glucosamine, cholesterol, and amino-acids. The oxygen content of all media was lowered either by cysteine or glutathione.3. Some excystation occurred in all media, the percentages were lowest in the inorganic fluid without bicarbonates, highest in fluid with bicarbonates plus all of the listed organic compounds, and intermediate in the inorganic bicarbonate fluid plus glucose. Amino-acids were not required for good percentages of excystation.4. Excystation occurred when cysts were isolated in medium with Trypanosoma cruzi.5. The data show that organic compounds in the medium are necessary for high percentages of excystation.

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