scholarly journals Immune Complexes Isolated from Patients with Pulmonary Tuberculosis Modulate the Activation and Function of Normal Granulocytes

2012 ◽  
Vol 19 (12) ◽  
pp. 1965-1971 ◽  
Author(s):  
P. Senbagavalli ◽  
J. Nancy Hilda ◽  
V. D. Ramanathan ◽  
V. Kumaraswami ◽  
Thomas B. Nutman ◽  
...  
Keyword(s):  
Immune Response ◽  
Pulmonary Tuberculosis ◽  
Intracellular Calcium ◽  
Immune Complexes ◽  
Oxidative Burst ◽  
Human Neutrophil ◽  
Platelet Activating Factor ◽  
The Other ◽  
And Function ◽  
Granulocyte Function

ABSTRACTCirculating immune complexes (ICs) are associated with the pathogenesis of several diseases. Very little is known about the effect of ICs on the host immune response in patients with tuberculosis (TB). The effects of ICs isolated from patients with TB in modulating the release of calcium, cytokines, and granular proteins were studied in normal granulocytes, as were their chemotactic, phagocytic, and oxidative burst processes. ICs from TB patients induced decreased production of cytokines and platelet-activating factor (PAF) from normal granulocytes. ICs from TB patients also induced enhanced chemotaxis and phagocytosis but caused diminished oxidative burst. This was accompanied by an increased release in intracellular calcium. On the other hand, ICs from TB patients induced increased release of the granular proteins human neutrophil peptides 1 to 3 (HNP1–3). Thus, ICs from patients with TB exhibit a profound effect on granulocyte function with activation of certain effector mechanisms and dampening of others.

scholarly journals CELLULAR DIFFERENTIATION OF THE IMMUNE SYSTEM OF MICE

1969 ◽  
Vol 129 (5) ◽  
pp. 935-951 ◽  
Author(s):  
G. M. Shearer ◽  
G. Cudkowicz
Keyword(s):  
Immune Response ◽  
Immune System ◽  
Cellular Differentiation ◽  
Marrow Cell ◽  
The Other ◽  
Cell Type ◽  
Series Of Experiments ◽  
And Function ◽  
Thymus Cells ◽  
Marrow Cells

Marrow cell suspensions of unprimed donor mice have been transplanted into X-irradiated syngeneic hosts. 5–46 days later, bone cavities and spleens contained regenerated cells of the immune system which required interaction with thymocytes (from intact donors) and antigen (SRBC) to form antigen-sensitive units (ASU) and to generate mature immunocytes. These cells were capable of differentiating either into direct or indirect hemolytic plaque-forming cells (PFC). The precursors of PFC regenerated earlier than the other cell type necessary for immunocompetence, the antigen-reactive cell (ARC). The latter was not found until 10 or more days after transplantation. Availability of ARC was inferred from PFC responses elicited by grafted mice challenged with SRBC at varying intervals. In a second series of experiments, graded numbers of marrow cells (ranging from 107 to 5 x 107) were transplanted with 5 x 107 or 108 thymocytes into irradiated mice, and SRBC were given 18 hr later. After 9–12 days the recipient spleens contained all or some of the following immunocytes: direct and indirect PFC, and hemagglutinating cluster-forming cells. The frequency of each immune response varied independently of the others, but in relation to the number of marrow cells grafted. This was interpreted to indicate that ASU formed in irradiated mice by interaction of marrow and thymus cells were similar to those of intact mice. In particular, they were specialized for the molecular class (IgM or IgG) and function (lysis or agglutination) of the antibody to be secreted by their descendent immunocytes. Hence, class-differentiation appeared to be conferred upon ASU by their marrow-derived components.

scholarly journals The impact of immune disturbances on the failure of antituberculosis treatment

2016 ◽  
Vol 89 (4) ◽  
pp. 493-498 ◽  
Author(s):  
Evelina Lesnic ◽  
Serghei Ghinda ◽  
Carmen Monica Pop
Keyword(s):  
Immune Response ◽  
T Cell ◽  
Pulmonary Tuberculosis ◽  
Treatment Failure ◽  
Humoral Immunity ◽  
Cellular Immunity ◽  
Immune Complexes ◽  
Treatment Initiation ◽  
Circulating Immune Complexes ◽  
Antituberculosis Treatment

Background and aim. Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis complex, with an evolution and treatment outcome determined by the interaction between the mycobacterial and human genotypes. Various deficiencies of innate immune response starting from the first encounter of M. tuberculosis with lung cells endanger host infection control due to decreased triggering of cellular immune resistance and disturbed humoral immunity. Disturbed cell mediated immunity, known as the basic immune response in tuberculous infection, contributes to the deficient generation of central necrosis granuloma, consequently being responsible for severe clinical aspects and low final outcome. The tuberculosis patient’s immune assessment is important before treatment initiation, for establishing the risk reduction measures and increasing success rate.Material and methods. The immune study was conducted on 54 new pulmonary tuberculosis cases with treatment failure, 34 new pulmonary tuberculosis cases that successfully ended the treatment and 50 healthy group individuals. Immune assays performed were: blastic transformation of lymphocytes induced by different antigens, quantitatitve assessment of cellular immunity through CD4+ T cell and CD8+ T cell phenotyping, humoral immunity - through immunoglobulin isotyping, innate resistance – through phagocyte activity of neutrophils, the titter of anti-tuberculosis antibodies and the serum level of circulating immune complexes. Investigations were performed at the onset the treatment and at the end of intensive phase of the standard anti-tuberculosis treatment.Results. Immune disturbances evidenced in patients with treatment failure were: important deficiencies of cellular immunity, hyperactivity of humoral immunity and deficiencies of innate immunity. High predictive value for treatment failure showed the indices: deficiency of T lymphocytes count (OR=62.5) and T helper count (OR=12.5), high level of circulating immune complexes (OR=9.801), deficiency of innate resistance (decreased phagocytating index OR=2.875).Conclusions. For increasing the treatment success rate, the study of immune disturbances must be performed before of antituberculosis treatment initiation , especially of cellular immunity for the early start of immune adaptive treatment.

Platelet Suruival and Function in Rats with Enhanced Thrombotic Tendency

1985 ◽  
Vol 54 (04) ◽  
pp. 739-743 ◽  
Author(s):  
Federica Delaini ◽  
Elisabetta Dejana ◽  
Ine Reyers ◽  
Elisa Vicenzi ◽  
Germana De Bellis Vitti ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Nephrotic Syndrome ◽  
Platelet Function ◽  
Laboratory Tests ◽  
Thrombus Formation ◽  
The Other ◽  
Mean Survival Time ◽  
The Mean ◽  
And Function ◽  
Thrombotic Tendency

SummaryWe have investigated the relevance of some laboratory tests of platelet function in predicting conditions of thrombotic tendency. For this purpose, we studied platelet survival, platelet aggregation in response to different stimuli, TxB2 and 6-keto-PGFlα production in serum of rats bearing a nephrotic syndrome induced by adriamycin. These animals show a heavy predisposition to the development of both arterial and venous thrombosis. The mean survival time was normal in nephrotic rats in comparison to controls. As to aggregation tests, a lower aggregating response was found in ADR-treated rats using ADP or collagen as stimulating agents. With arachidonic acid (AA) we observed similar aggregating responses at lower A A concentrations, whereas at higher AA concentrations a significantly lower response was found in nephrotic rats, despite their higher TxB2 production. Also TxB2 and 6-keto-PGFlα levels in serum of nephrotic rats were significantly higher than in controls. No consistent differences were found in PGI2-activity generated by vessels of control or nephrotic rats.These data show that platelet function may appear normal or even impaired in rats with a markedly increased thrombotic tendency. On the other hand, the significance of high TxB2 levels in connection with mechanisms leading to thrombus formation remains a controversial issue.

PARTICULARITIES OF THE IMMUNE RESPONSE IN PERSONS PROFESSIONNALLY EXPOSED TO BERYLLIUM

2016 ◽  
pp. 26-30
Author(s):  
K. I. Stosman ◽  
L. V. Lukovnikova
Keyword(s):  
Immune Response ◽  
Immune Complexes ◽  
Immunoglobulin E ◽  
Circulating Immune Complexes ◽  
Professional Contact ◽  
Beryllium Compounds

An examination was performed of 50 employees at an enterprise where they were in professional contact with beryllium. In most workers, it was detected an increase of interleukine-8, interferon- , growing level of immunoglobulin E and circulating immune complexes. It was shown that the contact with beryllium compounds leads to the interferon- level growth only in women. In men, alterations are identified in the direction of increased concentrations of common immunoglobulin E and circulating immune complexes.

Activation and function of hepatic NK cells in hepatitis B infection: an underinvestigated innate immune response

2005 ◽  
Vol 12 (1) ◽  
pp. 38-45 ◽  
Author(s):  
Y. Chen ◽  
H. Wei ◽  
B. Gao ◽  
Z. Hu ◽  
S. Zheng ◽  
...  
Keyword(s):  
Immune Response ◽  
Hepatitis B ◽  
Nk Cells ◽  
Innate Immune Response ◽  
Innate Immune ◽  
Hepatitis B Infection ◽  
And Function

scholarly journals Decitabine Promotes Modulation in Phenotype and Function of Monocytes and Macrophages That Drive Immune Response Regulation

Cells ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 868
Author(s):  
Fabiana Albani Zambuzi ◽  
Priscilla Mariane Cardoso-Silva ◽  
Ricardo Cardoso Castro ◽  
Caroline Fontanari ◽  
Flavio da Silva Emery ◽  
...  
Keyword(s):  
Immune Response ◽  
Hematological Malignancies ◽  
M2 Macrophage ◽  
M2 Polarization ◽  
Tnf Α ◽  
Monocytes And Macrophages ◽  
Ifn Γ ◽  
And Function ◽  
The Impact

Decitabine is an approved hypomethylating agent used for treating hematological malignancies. Although decitabine targets altered cells, epidrugs can trigger immunomodulatory effects, reinforcing the hypothesis of immunoregulation in treated patients. We therefore aimed to evaluate the impact of decitabine treatment on the phenotype and functions of monocytes and macrophages, which are pivotal cells of the innate immunity system. In vitro decitabine administration increased bacterial phagocytosis and IL-8 release, but impaired microbicidal activity of monocytes. In addition, during monocyte-to-macrophage differentiation, treatment promoted the M2-like profile, with increased expression of CD206 and ALOX15. Macrophages also demonstrated reduced infection control when exposed to Mycobacterium tuberculosis in vitro. However, cytokine production remained unchanged, indicating an atypical M2 macrophage. Furthermore, when macrophages were cocultured with lymphocytes, decitabine induced a reduction in the release of inflammatory cytokines such as IL-1β, TNF-α, and IFN-γ, maintaining IL-10 production, suggesting that decitabine could potentialize M2 polarization and might be considered as a therapeutic against the exacerbated immune response.

scholarly journals Relationship between the Geotourism Potential and Function in the Polish Part of the Roztocze Transboundary Biosphere Reserve

Geosciences ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 120
Author(s):  
Teresa Brzezińska-Wójcik
Keyword(s):  
Sustainable Development ◽  
Biosphere Reserve ◽  
The Other ◽  
Partial Indices ◽  
Function Index ◽  
Intensive Exploitation ◽  
Potential Indicator ◽  
Current Product ◽  
And Function ◽  
Further Development

The Polish part of the Roztocze Transboundary Biosphere Reserve area is characterized by diversified geotourism resources with relatively high value. However, their potential seems not to be fully used in the current product offer. The aim of the study was therefore to assess the spatial variability of the geotourism potential and function and to determine their interrelations in view of further development of geotourism in the Roztocze TBR and the perspective of creation of the “Kamienny Las na Roztoczu” geopark. The study was carried out with the use of the taxonomic method of multidimensional comparative analysis consisting of calculation and analysis of general, total, and partial indices of the geotourism potential and function in 22 communes. The results showed the highest total indicator of geotourism potential in two communes, i.e. Józefów and Krasnobród, and the highest value of the total geotourism function index in Krasnobród. The results of the analysis of the relationships between the geotourism potential and function indicate that the geotourism resources and products are fully used in terms of the development of the function only in Krasnobród commune. In turn, the value of the total geotourism function index in the Zwierzyniec commune exceeds the geotourism potential indicator, which implies that this area is overloaded by tourist movement. The total indicators of geotourism potential in the other communes, especially Józefów, Krasnobród, Lubycza Królewska, and Susiec, indicate the possibility of more intensive exploitation of geotourism resources in preparation of interesting products in compliance with the principles of sustainable development and, consequently, the development of the geotourism function.

Human neutrophil swelling induced by immune complexes and aggregated IgG

Inflammation ◽  
1981 ◽  
Vol 5 (3) ◽  
pp. 213-222 ◽  
Author(s):  
Eric R. Hurd ◽  
Yoshimi Hashimoto
Keyword(s):  
Immune Complexes ◽  
Human Neutrophil

Immune responses of Texel sheep to excretory/secretory products of adult Haemonchus contortus

Parasitology ◽  
1994 ◽  
Vol 108 (3) ◽  
pp. 351-357 ◽  
Author(s):  
H. D. F. H. Schallig ◽  
M. A. W. van Leeuwen ◽  
W. M. L. Hendrikx
Keyword(s):  
Immune Response ◽  
Immune Responses ◽  
Haemonchus Contortus ◽  
Lymphocyte Proliferation ◽  
The Other ◽  
Proliferation Index ◽  
Serum Samples ◽  
Immunoblotting Analysis ◽  
Molecular Weights ◽  
Secretory Products

SUMMARYThe excretory/secretory (E/S) products of adult Haemonchus contortus comprise of at least 15 polypeptides with molecular weights ranging from 10 to > 100 kDa. These E/S products induce an immune response in infected Texel sheep, as demonstrated by specific IgGI levels and a significant lymphocyte proliferation index. Moreover, immunoblotting analysis revealed that sera of primary H. contortus-infected sheep specifically recognize a 24 kDa E/S product. In addition, sera of challenged sheep react strongly with a 15 kDa E/S product. The other E/S products of H. contortus showed immunoreactivity with serum samples of Haemonchus-infected sheep as well as with samples of sheep harbouring other trichostrongylid infections. These cross-reacting epitopes are the main cause of the lack of specificity of an E/S material- based ELISA. This ELISA can differentiate Haemonchus infections from Nematodirus battus infections, but not from Ostertagia circumcincta or Trichostrongylus colubriformis infections.

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