scholarly journals Draft Genome Sequence of the First Confirmed Isolate of Multidrug-Resistant Mycobacterium tuberculosis in Tasmania

2017 ◽  
Vol 5 (44) ◽  
Author(s):  
Sanjay S. Gautam ◽  
Micheál Mac Aogáin ◽  
Ronan F. O’Toole
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Genome Sequence ◽  
Draft Genome ◽  
Multidrug Resistant ◽  
Whole Genome Sequence ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Content Type ◽  
Global Challenge ◽  
Mdr Tb

ABSTRACT The spread of multidrug-resistant (MDR) tuberculosis (TB) has become a major global challenge. In 2016, Tasmania recorded its first known incidence of MDR-TB. Here, we report the draft whole-genome sequence of the Mycobacterium tuberculosis isolate from this case, TASMDR1, and describe single-nucleotide polymorphisms associated with its drug resistance.

scholarly journals Draft Genome Sequence of Invasive Salmonella enterica Serovar Cannstatt Harboring mcr-1.1, Isolated from a Fatal Sepsis Case

2021 ◽  
Vol 10 (8) ◽  
Author(s):  
Wantana Paveenkittiporn ◽  
Watcharaporn Kamjumphol ◽  
Anusak Kerdsin
Keyword(s):  
Genome Sequence ◽  
Salmonella Enterica ◽  
Draft Genome ◽  
Genomic Analysis ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Whole Genome Sequence ◽  
Content Type ◽  
Fatal Sepsis ◽  
M 14

ABSTRACT Here, we report the whole-genome sequence of multidrug-resistant Salmonella enterica serovar Cannstatt harboring mcr-1.1, isolated from a fatal sepsis case. Genomic analysis revealed that the isolate was sequence type 2390 carrying mcr-1.1, blaCTX-M-14, aac(3)IId, aac(6′)Iaa, floR, qnrS1, sul2, tetA, and tetM. Three Inc plasmids were observed, including the IncX4 plasmid containing mcr-1.1.

scholarly journals Draft Genome Sequence of an Isolate of Extensively Drug-Resistant Mycobacterium tuberculosis from Nepal

2020 ◽  
Vol 9 (4) ◽  
Author(s):  
Sanjay S. Gautam ◽  
Kelvin W. C. Leong ◽  
Manoj Pradhan ◽  
Y. Ibotomba Singh ◽  
Sagar K. Rajbhandari ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Drug Resistant ◽  
Single Nucleotide ◽  
Content Type ◽  
Extensively Drug Resistant ◽  
Drug Resistant Phenotype ◽  
First Time

Extensively drug-resistant (XDR) Mycobacterium tuberculosis has become a challenge to the treatment of tuberculosis (TB) in several countries, including Nepal. Here, we report for the first time the draft genome sequence of an isolate of XDR-TB collected in Nepal and describe single-nucleotide variations associated with its extensively drug-resistant phenotype.

scholarly journals Draft Genome Sequence of Multidrug-Resistant Strain Citrobacter portucalensis MBTC-1222, Isolated from Uziza ( Piper guineense ) Leaves in Nigeria

2018 ◽  
Vol 6 (9) ◽  
Author(s):  
Etinosa O. Igbinosa ◽  
Jana Rathje ◽  
Diana Habermann ◽  
Erik Brinks ◽  
Gyu-Sung Cho ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Draft Genome ◽  
Noncoding Rnas ◽  
Multidrug Resistant ◽  
Whole Genome Sequence ◽  
Piper Guineense ◽  
Protein Coding ◽  
Antibiotic Resistant ◽  
Content Type ◽  
Multidrug Resistant Strain

ABSTRACT In this work, we report the draft whole-genome sequence of the multiply antibiotic-resistant Citrobacter portucalensis strain MBTC-1222 isolated from the uziza leafy vegetable in Nigeria. Sequence analysis showed the assembled genome size to be 4,881,935 bp, containing 4,603 protein-coding genes, 131 pseudogenes, 7 rRNAs, 74 tRNAs, and 9 noncoding RNAs (ncRNAs).

scholarly journals Whole-Genome Sequence of Acinetobacter baumannii Strain NUBRI-A, Isolated from a Hospitalized Patient in Khartoum, Sudan

2019 ◽  
Vol 8 (32) ◽  
Author(s):  
Sofia B. Mohamed ◽  
Mohamed Hassan ◽  
Abdalla Munir ◽  
Sumaya Kambal ◽  
Nusiba I. Abdalla ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Genome Sequence ◽  
Genomic Sequence ◽  
Draft Genome ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Hospital Patient ◽  
Content Type

Acinetobacter baumannii has emerged as an important pathogen leading to multiple nosocomial outbreaks. Here, we describe the genomic sequence of a multidrug-resistant Acinetobacter baumannii sequence type 164 (ST164) isolate from a hospital patient in Sudan. To our knowledge, this is the first reported draft genome of an A. baumannii strain isolated from Sudan.

scholarly journals Draft Genome Sequence of a Highly Heterozygous Yeast Strain from the Metschnikowia pulcherrima Subclade, UCD127

2018 ◽  
Vol 6 (25) ◽  
Author(s):  
Anjan Venkatesh ◽  
Anthony L. Murray ◽  
Adrian B. Boyle ◽  
Lisa Quinn Farrington ◽  
Timothy J. Maher ◽  
...  
Keyword(s):  
Mitochondrial Genome ◽  
Single Nucleotide Polymorphisms ◽  
Genome Sequence ◽  
Ribosomal Dna ◽  
Yeast Strain ◽  
Draft Genome ◽  
Nucleotide Polymorphisms ◽  
Diploid Strain ◽  
Single Nucleotide ◽  
Content Type

ABSTRACT Metschnikowia strain UCD127 was isolated from soil in Ireland and sequenced. It is a highly heterozygous diploid strain with 385,000 single nucleotide polymorphisms (SNPs). Its ribosomal DNA has the highest similarity to that of M. chrysoperlae, but its ACT1 and TEF1 loci and mitochondrial genome show affinity to those of M. fructicola, whose genome is significantly larger.

scholarly journals Whole-Genome Sequence Analysis of Multidrug-Resistant Enterobacter hormaechei Isolated from Imported Retail Shrimp

2020 ◽  
Vol 9 (50) ◽  
Author(s):  
Nagaraju Indugu ◽  
Laxmi Sharma ◽  
Charlene R. Jackson ◽  
Prashant Singh
Keyword(s):  
Genome Sequence ◽  
Draft Genome ◽  
Antibiotic Resistance Genes ◽  
Multidrug Resistant ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Genome Sequence Analysis ◽  
Content Type ◽  
Shrimp Sample ◽  
Enterobacter Hormaechei

ABSTRACT Here, we announce the draft genome sequence of Enterobacter hormaechei 2B-MC1, isolated from a shrimp sample collected from a farmer’s market in Atlanta, Georgia. The assembled genome sequence observed was 4,661,561 bp long with a G+C content of 55.3%. The isolate harbored sul1, sul2, qnrA1, oqxB, dfrA23, blaACT, floR, fosA, tet(A), aph(6)-Id, and aph(3″)-Ib antibiotic resistance genes.

scholarly journals Draft Genome Sequence of Nitrosococcus oceani Strain NS58, a Marine Ammonia-Oxidizing Gammaproteobacterium Isolated from Tokyo Bay Sediment

2019 ◽  
Vol 8 (35) ◽  
Author(s):  
Hideo Dohra ◽  
Kentaro Arai ◽  
Hidetoshi Urakawa ◽  
Taketomo Fujiwara
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Tokyo Bay ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Content Type

We report a draft genome sequence of Nitrosococcus oceani strain NS58, isolated from Tokyo Bay sediment. The genome sequence of strain NS58 was nearly identical (>99.99%) to those of other strains of N. oceani isolated from different ocean regions. Only nine single-nucleotide polymorphisms were identified between N. oceani ATCC 19707T and NS58.

scholarly journals Whole-Genome Sequence of a Reemerging Listeria monocytogenes Serovar 1/2a Strain in Central Italy

2018 ◽  
Vol 7 (23) ◽  
Author(s):  
Massimiliano Orsini ◽  
Marina Torresi ◽  
Claudio Patavino ◽  
Patrizia Centorame ◽  
Antonio Rinaldi ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Single Nucleotide Polymorphisms ◽  
Genome Sequence ◽  
Central Italy ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Content Type

We report the whole-genome sequence of a Listeria monocytogenes strain isolated from a child in central Italy. Interestingly, the sequence showed a difference of only 13 single-nucleotide polymorphisms (SNPs) from a strain responsible for a severe listeriosis outbreak that occurred between January 2015 and March 2016 in the same region.

scholarly journals Comparison of In Vitro Activity and MIC Distributions between the Novel Oxazolidinone Delpazolid and Linezolid against Multidrug-Resistant and Extensively Drug-Resistant Mycobacterium tuberculosis in China

2018 ◽  
Vol 62 (8) ◽  
Author(s):  
Zhaojing Zong ◽  
Wei Jing ◽  
Jin Shi ◽  
Shu'an Wen ◽  
Tingting Zhang ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Novel Mutation ◽  
Multidrug Resistant ◽  
23S Rrna ◽  
Drug Resistant ◽  
Content Type ◽  
Extensively Drug Resistant ◽  
Significant Difference ◽  
Mdr Tb

ABSTRACT Oxazolidinones are efficacious in treating mycobacterial infections, including tuberculosis (TB) caused by drug-resistant Mycobacterium tuberculosis. In this study, we compared the in vitro activities and MIC distributions of delpazolid, a novel oxazolidinone, and linezolid against multidrug-resistant TB (MDR-TB) and extensively drug-resistant TB (XDR-TB) in China. Additionally, genetic mutations in 23S rRNA, rplC, and rplD genes were analyzed to reveal potential mechanisms underlying the observed oxazolidinone resistance. A total of 240 M. tuberculosis isolates were included in this study, including 120 MDR-TB isolates and 120 XDR-TB isolates. Overall, linezolid and delpazolid MIC90 values for M. tuberculosis isolates were 0.25 mg/liter and 0.5 mg/liter, respectively. Based on visual inspection, we tentatively set epidemiological cutoff (ECOFF) values for MIC determinations for linezolid and delpazolid at 1.0 mg/liter and 2.0 mg/liter, respectively. Although no significant difference in resistance rates was observed between linezolid and delpazolid among XDR-TB isolates (P > 0.05), statistical analysis revealed a significantly greater proportion of linezolid-resistant isolates than delpazolid-resistant isolates within the MDR-TB group (P = 0.036). Seven (53.85%) of 13 linezolid-resistant isolates were found to harbor mutations within the three target genes. Additionally, 1 isolate exhibited an amino acid substitution (Arg126His) within the protein encoded by rplD that contributed to high-level resistance to linezolid (MIC of >16 mg/liter), compared to a delpazolid MIC of 0.25. In conclusion, in vitro susceptibility testing revealed that delpazolid antibacterial activity was comparable to that of linezolid. A novel mutation within rplD that endowed M. tuberculosis with linezolid, but not delpazolid, resistance was identified.

scholarly journals Draft Genome Sequence of Acholeplasma laidlawii Isolated from the Conjunctiva of a Heifer with Infectious Bovine Keratoconjunctivitis

2021 ◽  
Vol 10 (4) ◽  
Author(s):  
Gloria Gioia ◽  
Maria F. Addis ◽  
Laura B. Goodman ◽  
Patrick K. Mitchell ◽  
Belinda Thompson ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Content Type ◽  
Infectious Bovine Keratoconjunctivitis ◽  
Acholeplasma Laidlawii

ABSTRACT Acholeplasma laidlawii can be isolated from cattle environments and different body sites of bovines. It is still under evaluation if A. laidlawii acts as a primary pathogen. Here, we present the whole-genome sequence of A. laidlawii isolated from the conjunctiva of a heifer with infectious bovine keratoconjunctivitis.

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