scholarly journals Genome Sequence ofRhizobium sullaeHCNT1 Isolated fromHedysarum coronariumNodules and Featuring Peculiar Denitrification Phenotypes

2018 ◽  
Vol 6 (4) ◽  
Author(s):  
B. de Diego-Diaz ◽  
L. Treu ◽  
S. Campanaro ◽  
V. da Silva Duarte ◽  
M. Basaglia ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Reductase Activity ◽  
Root Nodules ◽  
High Sensitivity ◽  
Anaerobic Conditions ◽  
Hedysarum Coronarium

ABSTRACTThe genome sequence ofRhizobium sullaestrain HCNT1, isolated from root nodules of the legumeHedysarum coronariumgrowing in wild stands in Tuscany, Italy, is described here. Unlike otherR. sullaestrains, this isolate features a truncated denitrification pathway lacking NO/N2O reductase activity and displaying high sensitivity to nitrite under anaerobic conditions.

scholarly journals Chromate Reduction by a Pseudomonad Isolated from a Site Contaminated with Chromated Copper Arsenate

2001 ◽  
Vol 67 (3) ◽  
pp. 1076-1084 ◽  
Author(s):  
Jeff McLean ◽  
Terry J. Beveridge
Keyword(s):  
Reductase Activity ◽  
Wood Preservation ◽  
Chromate Reduction ◽  
Anaerobic Conditions ◽  
Toxic Heavy Metal ◽  
Metal Metal ◽  
A Site ◽  
Anaerobic Reduction ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

ABSTRACT A pseudomonad (CRB5) isolated from a decommissioned wood preservation site reduced toxic chromate [Cr(VI)] to an insoluble Cr(III) precipitate under aerobic and anaerobic conditions. CRB5 tolerated up to 520 mg of Cr(VI) liter−1 and reduced chromate in the presence of copper and arsenate. Under anaerobic conditions it also reduced Co(III) and U(VI), partially internalizing each metal. Metal precipitates were also found on the surface of the outer membrane and (sometimes) on a capsule. The results showed that chromate reduction by CRB5 was mediated by a soluble enzyme that was largely contained in the cytoplasm but also found outside of the cells. The crude reductase activity in the soluble fraction showed aKm of 23 mg liter−1 (437 μM) and a V max of 0.98 mg of Cr h−1 mg of protein−1 (317 nmol min−1 mg of protein−1). Minor membrane-associated Cr(VI) reduction under anaerobiosis may account for anaerobic reduction of chromate under nongrowth conditions with an organic electron donor present. Chromate reduction under both aerobic and anaerobic conditions may be a detoxification strategy for the bacterium which could be exploited to bioremediate chromate-contaminated or other toxic heavy metal-contaminated environments.

A new water-soluble bacterial NADH: fumarate oxidoreductase

2020 ◽  
Vol 367 (20) ◽  
Author(s):  
Yulia V Bertsova ◽  
Ilya P Oleynikov ◽  
Alexander V Bogachev
Keyword(s):  
Nadh Dehydrogenase ◽  
Reductase Activity ◽  
Domain Architecture ◽  
Water Soluble ◽  
Fumarate Reductase ◽  
Anaerobic Conditions ◽  
New Type ◽  
Prosthetic Groups ◽  
Low Rate ◽  
Covalently Bound

ABSTRACT The cytoplasmic fumarate reductase of Klebsiella pneumoniae (FRD) is a monomeric protein which contains three prosthetic groups: noncovalently bound FMN and FAD plus a covalently bound FMN. In the present work, NADH is revealed to be an inherent electron donor for this enzyme. We found that the fumarate reductase activity of FRD significantly exceeds its NADH dehydrogenase activity. During the catalysis of NADH:fumarate oxidoreductase reaction, FRD turnover is limited by a very low rate (∼10/s) of electron transfer between the noncovalently and covalently bound FMN moieties. Induction of FRD synthesis in K. pneumoniae cells was observed only under anaerobic conditions in the presence of fumarate or malate. Enzymes with the FRD-like domain architecture are widely distributed among various bacteria and apparently comprise a new type of water-soluble NADH:fumarate oxidoreductases.

scholarly journals Bio-chemical characterization of rhizobia isolated from root nodules of Velvet bean (Mucuna pruriens L.)

Our Nature ◽  
2017 ◽  
Vol 15 (1-2) ◽  
pp. 7-12 ◽  
Author(s):  
Som Prasad Paudyal ◽  
Vimal NP Gupta
Keyword(s):  
Rhizobium Meliloti ◽  
Root Nodules ◽  
Chemical Characterization ◽  
High Sensitivity ◽  
Mucuna Pruriens ◽  
Velvet Bean ◽  
Trigonella Foenum Graecum ◽  
Antibiotics Sensitivity ◽  
The Himalaya

Rhizobia are the symbiotic bacteria found in the soil which have potential ability to convert atmospheric di-nitrogen into usable form. A total of ten rhizobial strains were isolated from the root nodules of a medicinal legume Mucuna pruriens (L.) that commonly grow in the foothills of the Himalaya. All the ten   strains isolated from different locations of same area were morphologically, biochemically and physiologically characterized based on the Bergey’s Manual of systematic Bacteriology. They were tested for the antibiotics sensitivity. The isolates showed high sensitivity to amoxicillin and least to erythromycin. Authentication test was done in eleven legumes but shown nodulations only in Trigonella foenum-graecum, Mucuna pruriens and Medicago sativa. The morphology, physiology, biochemical and infection test studies carried out justifies that the bacteria isolated belonged to the species of Rhizobium meliloti.

scholarly journals Unusual Aldehyde Reductase Activity for Production of Full-length Fatty Alcohol by Cyanobacterial Aldehyde Deformylating Oxygenase  

2020 ◽  
Author(s):  
Supacha Buttranon ◽  
Pattarawan Intasian ◽  
Nidar Treesukkasem ◽  
Juthamas Jaroensuk ◽  
Somchart Maenpuen ◽  
...  
Keyword(s):  
Binding Site ◽  
Reductase Activity ◽  
Md Simulations ◽  
Full Length ◽  
Fatty Alcohols ◽  
Aldehyde Reductase ◽  
Anaerobic Conditions ◽  
Alcohol Production ◽  
Reduction Activity ◽  
Fatty Aldehydes

Abstract Background: Aldehyde-deformylating oxygenase (ADO) is a non-heme di-iron enzyme that catalyzes deformylation of aldehydes to generate alkanes/alkenes. In this study, we report for the first time that under anaerobic or limited oxygen conditions, Prochlorococcus marinus (PmADO) can generate full-length fatty alcohols from fatty aldehydes without eliminating a carbon unit. Results: Unlike the native activity of ADO which requires electrons from the Fd/FNR electron transfer complex, the aldehyde reduction activity of ADO requires only NADPH. Our results demonstrated that yield of alcohol products can be affected by oxygen concentration and type of aldehyde. Under O2-scant conditions (10-15%), yields of octanol and dodecanol were around 40-60% and could be increased up to 80% under strict anaerobic conditions (>0.0004%). Unexpectedly, Fe2+ cofactor is not involved in the aldehyde reductase activity of PmADO because yields of alcohols obtained from holo- and apo-enzymes were similar under anaerobic conditions. The direct hydride transfer activity of PmADO is highly specific to substrates; NADPH not NADH can be used as a reductant to reduce medium-chain fatty aldehydes (C6-C10) with decanal as the most preferred substrate (the highest kcat/Km value with 98% bioconversion yield). Molecular dynamics (MD) simulations was used to identify a binding site of NADPH which is located close to the aldehyde binding site. In the metabolic engineered cells containing PmADO, dual activities of alkane and alcohol production could be detected. Conclusion: The findings reported herein highlight a new activity of PmADO which may be applied as a biocatalyst for industrial synthesis of fatty alcohols in the future.

scholarly journals Characterisation of rhizobia nodulating Galega officinalis (goats rue) and Hedysarum coronarium (sulla)

2012 ◽  
Vol 65 ◽  
pp. 192-196 ◽  
Author(s):  
W.Y.Y. Liu ◽  
H.J. Ridgway ◽  
T.K. James ◽  
M. Premaratne ◽  
M. Andrews
Keyword(s):  
New Zealand ◽  
Root Nodules ◽  
Housekeeping Genes ◽  
Symbiotic Bacteria ◽  
Legume Species ◽  
Bacterial Strains ◽  
Agrobacterium Vitis ◽  
Galega Officinalis ◽  
Hedysarum Coronarium ◽  
Low Nitrogen

Many legumes can colonise low nitrogen (N) soils due to their ability to fix atmospheric N2 via symbiotic bacteria in root nodules Galega officinalis and Hedysarum coronarium are legumes that have naturalised and become common weeds in New Zealand Previous work outside of New Zealand indicated that they only form effective nodules with their respective symbionts Rhizobium galegae and R sullae Here analysis of 16S rRNA and housekeeping genes and plant nodulation tests were carried out on five selected bacterial strains isolated from root nodules of both legumes sampled at one site each Only Rhizobium galegae strains were isolated from G officinalis and selected strains induced effective nodules when reinoculated onto the host plant Agrobacterium vitis R galegae and R sullae strains were isolated from nodules of H coronarium but only R sullae induced effective nodules on this plant Results agree with previous reports that these legume species are highly specific in the rhizobia they form effective nodules with but further work is required to confirm this

Whole-genome sequence-guided PCR for the rapid identification of thePseudomonas aeruginosa ST175 high-risk clone directly from clinical samples

2020 ◽  
Author(s):  
Gabriel Cabot ◽  
Paula Lara-Esbrí ◽  
Xavier Mulet ◽  
Antonio Oliver
Keyword(s):  
High Risk ◽  
Sensitivity And Specificity ◽  
Genome Sequence ◽  
Treatment Strategies ◽  
High Sensitivity ◽  
Whole Genome Sequence ◽  
Clinical Samples ◽  
Whole Genome ◽  
Pcr Assay ◽  
Pcr Targeting

Abstract Objectives Pseudomonas aeruginosa frequently show MDR/XDR profiles, which are associated with worldwide-disseminated high-risk clones (HRCs). We developed a PCR assay for the detection in clinical samples of ST175, an HRC that is widespread in European countries. Methods The whole-genome sequence was obtained for one ST175 isolate using a PacBio RSII sequencer. Reads from multiple isolates belonging to ST175 and the PAO1 reference strain were mapped against the ST175 genome to identify potentially specific regions. Once curated, using the BLAST database to search for the presence of those regions in any other organism, we designed a specific PCR for the detection of ST175. Results Assembly of the ST175 PacBio-sequenced genome resulted in three contigs with a total length of 7 087 985 bases, encoding 6566 coding sequences. Specific regions for ST175 genomes were detected and a PCR targeting a 318 bp fragment located within a 3177 bp ORF coding for a putative reverse transcriptase was designed. The PCR test was first evaluatedin silico against 229 XDRP. aeruginosa genomes (73 ST175) from two multicentre studies, yielding 100% sensitivity and specificity. Then, the PCR was evaluatedin vitro in 25 isolates (12 ST175) and in 120 clinical samples (30 urine samples, 30 blood cultures, 30 sputum samples and 30 rectal swabs) of which 10% contained ST175, yielding again 100% sensitivity and specificity. Conclusions The PCR assay developed, showing high sensitivity and specificity for the detection of the ST175 HRC directly from clinical samples, could become a useful tool for guiding infection control and treatment strategies in areas with a high prevalence of this clone.

scholarly journals Draft Genome Sequence of Magnetospirillum sp. Strain 15-1, a Denitrifying Toluene Degrader Isolated from a Planted Fixed-Bed Reactor

2017 ◽  
Vol 5 (32) ◽  
Author(s):  
Ingrid Meyer-Cifuentes ◽  
Stefan Fiedler ◽  
Jochen A. Müller ◽  
Uwe Kappelmeyer ◽  
Ines Mäusezahl ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Genome Assembly ◽  
Draft Genome ◽  
Fixed Bed ◽  
Fixed Bed Reactor ◽  
Draft Genome Sequence ◽  
Toluene Degradation ◽  
Anaerobic Conditions ◽  
Coding Sequences

ABSTRACT Here, we report the draft genome sequence of Magnetospirillum sp. 15-1. This strain was isolated from a planted fixed-bed reactor based on its ability to degrade toluene under anaerobic conditions. The genome assembly consists of 5.4 Mb in 28 contigs and 5,095 coding sequences containing the genes involved in anaerobic toluene degradation.

scholarly journals Genome Sequence of Rhizobium etli CNPAF512, a Nitrogen-Fixing Symbiont Isolated from Bean Root Nodules in Brazil

2011 ◽  
Vol 193 (12) ◽  
pp. 3158-3159 ◽  
Author(s):  
M. Fauvart ◽  
A. Sanchez-Rodriguez ◽  
S. Beullens ◽  
K. Marchal ◽  
J. Michiels
Keyword(s):  
Genome Sequence ◽  
Root Nodules ◽  
Nitrogen Fixing ◽  
Rhizobium Etli ◽  
Bean Root

scholarly journals Permanent Draft Genome Sequence forFrankiasp.Strain EI5c, a Single-Spore Isolate of a Nitrogen-Fixing Actinobacterium, Isolated from the Root Nodules ofElaeagnus angustifolia

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Timothy D’Angelo ◽  
Rediet Oshone ◽  
Feseha Abebe-Akele ◽  
Stephen Simpson ◽  
Krystalynne Morris ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Root Nodules ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Nitrogen Fixing ◽  
Single Spore ◽  
Single Spore Isolate ◽  
Protein Encoding ◽  
Candidate Protein ◽  
Encoding Genes

Frankiasp. strain EI5c is a member ofFrankialineage III, which is able to reinfect plants of theEleagnaceae,Rhamnaceae,Myricaceae, andGymnostoma, as well as the genusAlnus. Here, we report the 6.6-Mbp draft genome sequence ofFrankiasp. strain EI5c with a G+C content of 72.14 % and 5,458 candidate protein-encoding genes.

Sign in / Sign up

Export Citation Format

Share Document