scholarly journals Local and Systemic Responses in Matrix Metalloproteinase 8-Deficient Mice during Porphyromonas gingivalis-Induced Periodontitis

2008 ◽  
Vol 77 (2) ◽  
pp. 850-859 ◽  
Author(s):  
Heidi Kuula ◽  
Tuula Salo ◽  
Emma Pirilä ◽  
Anita M. Tuomainen ◽  
Matti Jauhiainen ◽  
...  
Keyword(s):  
Bone Loss ◽  
Matrix Metalloproteinase ◽  
Porphyromonas Gingivalis ◽  
Alveolar Bone ◽  
Inflammatory Responses ◽  
Density Lipoprotein ◽  
Alveolar Bone Loss ◽  
Type I ◽  
Site Specific ◽  
Vldl Particle

ABSTRACT Periodontitis is a bacterium-induced chronic inflammation that destroys tissues that attach teeth to jaw bone. Pathologically excessive matrix metalloproteinase 8 (MMP-8) is among the key players in periodontal destruction by initiating type I collagen degradation. We studied MMP-8 in Porphyromonas gingivalis-induced periodontitis by using MMP-8-deficient (MMP8 −/− ) and wild-type (WT) mice. Alveolar bone loss, inflammatory mediator expression, serum immunoglobulin, and lipoprotein responses were investigated to clarify the role of MMP-8 in periodontitis and systemic inflammatory responses. P. gingivalis infection induced accelerated site-specific alveolar bone loss in both MMP8 −/− and WT mice relative to uninfected mice. The most extensive bone degradation took place in the P. gingivalis-infected MMP8 −/− group. Surprisingly, MMP-8 significantly attenuated (P < 0.05) P. gingivalis-induced site-specific alveolar bone loss. Increased alveolar bone loss in P. gingivalis-infected MMP8 −/− and WT mice was associated with increase in gingival neutrophil elastase production. Serum lipoprotein analysis demonstrated changes in the distribution of high-density lipoprotein (HDL) and very-low-density lipoprotein (VLDL) particles; unlike the WT mice, the MMP8 −/− mice underwent a shift toward a smaller HDL/VLDL particle sizes. P. gingivalis infection increased the HDL/VLDL particle size in the MMP8 −/− mice, which is an indicator of lipoprotein responses during systemic inflammation. Serum total lipopolysaccharide activity and the immunoglobulin G-class antibody level in response to P. gingivalis were significantly elevated in both infected mice groups. Thus, MMP-8 appears to act in a protective manner inhibiting the development of bacterium-induced periodontal tissue destruction, possibly through the processing anti-inflammatory cytokines and chemokines. Bacterium-induced periodontitis, especially in MMP8 −/− mice, is associated with systemic inflammatory and lipoprotein changes that are likely involved in early atherosclerosis.

scholarly journals Carotid sinus nerve stimulation attenuates alveolar bone loss and inflammation in experimental periodontitis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Aline Barbosa Ribeiro ◽  
Fernanda Brognara ◽  
Josiane Fernandes da Silva ◽  
Jaci Airton Castania ◽  
Patrícia Garani Fernandes ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Bone Loss ◽  
Alveolar Bone ◽  
Carotid Sinus ◽  
Inflammatory Responses ◽  
Periodontal Diseases ◽  
Alveolar Bone Loss ◽  
Carotid Sinus Nerve ◽  
Mandibular Molar ◽  
Inflammatory Processes

Abstract Baroreceptor and chemoreceptor reflexes modulate inflammatory responses. However, whether these reflexes attenuate periodontal diseases has been poorly examined. Thus, the present study determined the effects of electrical activation of the carotid sinus nerve (CSN) in rats with periodontitis. We hypothesized that activation of the baro and chemoreflexes attenuates alveolar bone loss and the associated inflammatory processes. Electrodes were implanted around the CSN, and bilateral ligation of the first mandibular molar was performed to, respectively, stimulate the CNS and induce periodontitis. The CSN was stimulated daily for 10 min, during nine days, in unanesthetized animals. On the eighth day, a catheter was inserted into the left femoral artery and, in the next day, the arterial pressure was recorded. Effectiveness of the CNS electrical stimulation was confirmed by hypotensive responses, which was followed by the collection of a blood sample, gingival tissue, and jaw. Long-term (9 days) electrical stimulation of the CSN attenuated bone loss and the histological damage around the first molar. In addition, the CSN stimulation also reduced the gingival and plasma pro-inflammatory cytokines induced by periodontitis. Thus, CSN stimulation has a protective effect on the development of periodontal disease mitigating alveolar bone loss and inflammatory processes.

The interleukin-10 knockout mouse is highly susceptible to Porphyromonas gingivalis-induced alveolar bone loss

2004 ◽  
Vol 39 (6) ◽  
pp. 432-441 ◽  
Author(s):  
Hajime Sasaki ◽  
Yoshimasa Okamatsu ◽  
Toshihisa Kawai ◽  
Ralph Kent ◽  
Martin Taubman ◽  
...  
Keyword(s):  
Bone Loss ◽  
Porphyromonas Gingivalis ◽  
Knockout Mouse ◽  
Alveolar Bone ◽  
Interleukin 10 ◽  
Alveolar Bone Loss

scholarly journals Micromolar sodium fluoride mediates anti-osteoclastogenesis in Porphyromonas gingivalis-induced alveolar bone loss

2015 ◽  
Vol 7 (4) ◽  
pp. 242-249 ◽  
Author(s):  
Ujjal K Bhawal ◽  
Hye-Jin Lee ◽  
Kazumune Arikawa ◽  
Michiharu Shimosaka ◽  
Masatoshi Suzuki ◽  
...  
Keyword(s):  
Bone Loss ◽  
Porphyromonas Gingivalis ◽  
Sodium Fluoride ◽  
Alveolar Bone ◽  
Alveolar Bone Loss

scholarly journals Kgp and RgpB, but Not RgpA, Are Important for Porphyromonas gingivalis Virulence in the Murine Periodontitis Model

2007 ◽  
Vol 75 (3) ◽  
pp. 1436-1442 ◽  
Author(s):  
Rishi D. Pathirana ◽  
Neil M. O'Brien-Simpson ◽  
Gail C. Brammar ◽  
Nada Slakeski ◽  
Eric C. Reynolds
Keyword(s):  
Bone Loss ◽  
Porphyromonas Gingivalis ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bacterial Cells ◽  
Wild Type ◽  
Isogenic Mutant ◽  
Specific Immunoglobulin ◽  
Isogenic Mutants

ABSTRACT The contributions of three proteinase genes (rgpA, rgpB, and kgp) to the virulence of Porphyromonas gingivalis W50 were investigated in the murine periodontitis model. Mice were orally inoculated with eight doses (1 × 1010 cells per dose) of rgpA, rgpB, kgp, rgpA rgpB, or rgpA rgpB kgp isogenic mutants, and the level of alveolar bone loss, immune response induced, and number of bacterial cells per half maxilla were compared with those of animals inoculated with wild-type P. gingivalis. The kgp, rgpB, rgpA rgpB, and rgpA rgpB kgp isogenic mutants induced significantly (P < 0.05) less bone loss than the rgpA isogenic mutant and the wild type did, and the virulence of the rgpA isogenic mutant and the wild type were not significantly different. Mice inoculated with the wild type or the rgpA isogenic mutant exhibited significantly (P < 0.01) more P. gingivalis cells per half maxilla than mice inoculated with rgpB, kgp, rgpA rgpB, and rgpA rgpB kgp isogenic mutants or nonchallenged mice did, as determined using real-time PCR. A significant positive correlation was found between the number of P. gingivalis cells detected per half maxilla and the amount of alveolar bone loss induced. Enzyme-linked immunosorbent assay results showed that each isogenic mutant and the wild type induced a predominant P. gingivalis antigen-specific immunoglobulin G3 (IgG3) response. Furthermore, the kgp and rgpA rgpB kgp isogenic mutants induced significantly (P < 0.05) lower IgG3 antibody responses than the responses induced by the wild type or the rgpA, rgpB, and rgpA rgpB isogenic mutants. The results suggest that the order in which the proteinases contribute to the virulence of P. gingivalis in the murine periodontitis model is Kgp ≥ RgpB ≫ RgpA.

Blockade of sympathetic β-receptors inhibits Porphyromonas gingivalis-induced alveolar bone loss in an experimental rat periodontitis model

2010 ◽  
Vol 55 (7) ◽  
pp. 502-508 ◽  
Author(s):  
Yuka Okada ◽  
Nobushiro Hamada ◽  
Yul Kim ◽  
Yusuke Takahashi ◽  
Kenichi Sasaguri ◽  
...  
Keyword(s):  
Bone Loss ◽  
Porphyromonas Gingivalis ◽  
Alveolar Bone ◽  
Alveolar Bone Loss

scholarly journals Mixed lineage kinase domain-like pseudokinase mediated necroptosis aggravates periodontitis progression

2021 ◽  
Author(s):  
Yanan Yang ◽  
Lingxia Wang ◽  
Haibing Zhang ◽  
Lijun Luo
Keyword(s):  
Bone Marrow ◽  
Bone Loss ◽  
Porphyromonas Gingivalis ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Mrna Levels ◽  
Genetic Ablation ◽  
Osteoclast Activation

Abstract Necroptosis is a form of cell death that is reportedly involved in the pathogenesis of periodontitis. However, the role of Mlkl-involved necroptosis remains unclear. Herein, we aim to explore the role of MLKL-mediated necroptosis in periodontitis in vitro and in vivo. Expression of RIPK3, MLKL, and phosphorylated MLKL is observed in gingival tissues obtained from healthy subjects or patients with periodontitis. Viability of Porphyromonas gingivalis lipopolysaccharide (LPS-Pg)-treated cells was detected. In wild type or Mlkl deficiency mice with ligature-induced periodontitis, alveolar bone loss and osteoclast activation were assessed. mRNA levels of inflammatory cytokines in bone marrow-derived macrophages were tested by qRT-PCR. Increased expression of RIPK3, MLKL, and phosphorylated MLKL is observed in gingival tissues obtained from patients with periodontitis. Porphyromonas gingivalis lipopolysaccharide (LPS-Pg)-treated cells developed necroptosis after caspase inhibition and negatively regulated the NF-κB signaling pathway. In mice with ligature-induced periodontitis, Mlkl deficiency reduced alveolar bone loss and weakened osteoclast activation. Furthermore, genetic ablation of Mlkl in LPS-Pg-treated bone marrow-derived macrophages increased the mRNA levels of tumor necrosis factor-α, interleukin (Il)-1β, Il-6, cyclooxygenase 2, matrix metalloproteinase 9, and receptor activator of nuclear factor kappa-B ligand. Our data indicated that MLKL-mediated necroptosis aggravates the development of periodontitis in a Mlkl-deficient mouse. And this will provide a new sight for the understanding of etiology and therapies of periodontitis.

scholarly journals Mast cells contribute to alveolar bone loss in Spontaneously Hypertensive Rats with periodontal disease regulating cytokines production

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0247372
Author(s):  
Victor Gustavo Balera Brito ◽  
Mariana Sousa Patrocinio ◽  
Maria Carolina Linjardi Sousa ◽  
Ayná Emanuelli Alves Barreto ◽  
Sabrina Cruz Tfaile Frasnelli ◽  
...  
Keyword(s):  
Mast Cells ◽  
Bone Loss ◽  
Periodontal Disease ◽  
Spontaneously Hypertensive Rats ◽  
Alveolar Bone ◽  
Inflammatory Responses ◽  
Alveolar Bone Loss ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Inflammatory Status

Mast cells (MCs) play a pivotal role in inflammatory responses and had been studied in inflammatory bone disorders, however, their role in alveolar bone loss induced by periodontal disease (PD) is not yet fully understood. We, therefore, aimed to evaluate the effects of MCs depletion in the PD-induced alveolar bone loss in Wistar (W) and Spontaneously Hypertensive Rats (SHRs). PD was induced by ligating the lower first molars with silk thread one day after the MCs depletion, by the pre-treatment with compound 48/80 for 4 days. After 15 days of PD induction, the hemi-mandibles were surgically collected for qRT-PCR, histological analyses, immunostaining, and ELISA. Systolic blood pressure (SBP) was verified by tail plethysmography to confirm the hypertensive status, and SHR presented SBP >150 mmHg, and previous MC depletion alone or associated with PD did not alter this parameter. SHRs showed a more severe alveolar bone loss compared to W, and MC depletion significantly inhibited this response in both strains, with a more significant response in SHRs. MCs were less abundant in 48/80+PD groups, thus validating the previous MCs depletion in our model. PD increased the number of MC in the gingival tissue of SHR. Cytokine production (TNF-α, IL-6, IL-1β, and CXCL3) was constitutively higher in SHR and increased further after PD, which was also significantly reduced in the MCs-depleted animals. PD led to an increased expression of Opn, Rankl, Rank, Vtn, Itga5, Itgb5, Trap, and Ctsk in the mandible of W and SHRs, which was reversed in MCs-depleted animals. These results suggest that MCs significantly contributes to the PD-induced alveolar bone resorption, especially in the SHR, which is associated with a more severe PD progression compared to Wistar, partly explained by these cells contribution to the inflammatory status and mediator production, stimulating osteoclast-related response markers, which were reduced after MC depletion in our experimental model.

Spirulina maxima reduces inflammation and alveolar bone loss in Porphyromonas gingivalis-induced periodontitis

Phytomedicine ◽  
2021 ◽  
Vol 81 ◽  
pp. 153420
Author(s):  
Min Song Kang ◽  
Ji-Hoi Moon ◽  
Sang Cheol Park ◽  
Young Pyo Jang ◽  
Se Young Choung
Keyword(s):  
Bone Loss ◽  
Porphyromonas Gingivalis ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Spirulina Maxima
Sign in / Sign up

Export Citation Format

Share Document