Synthesis of Microcapsule by Staphylococcus aureus Is Not Responsive to Environmental Phosphate Concentrations

1998 ◽  
Vol 66 (8) ◽  
pp. 4004-4007 ◽  
Author(s):  
Karen F. Fox ◽  
George C. Stewart ◽  
Alvin Fox
Keyword(s):  
Mass Spectrometry ◽  
Staphylococcus Aureus ◽  
Teichoic Acid ◽  
Acid Synthesis ◽  
Growth Conditions ◽  
Phosphate Concentration ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Teichuronic Acid ◽  
Environmental Component

ABSTRACT The polysaccharide microcapsule of Staphylococcus aureus has been reported to be differentially expressed depending on growth conditions, with phosphate concentration being the critical environmental component. This study evaluated the effect of growth of a serotype 8 strain of S. aureus in phosphate-replete and phosphate-limiting media on microcapsule production. The presence of the cell wall polymers microcapsule and teichoic acid was measured by both gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry. Production of microcapsule was unaffected by changes in the environmental phosphate concentration. There was, additionally, no evidence for a shift from teichoic acid to teichuronic acid synthesis.

Comprehensive analysis of metabolites in Corynebacterium glutamicum by gas chromatography/mass spectrometry

2004 ◽  
Vol 385 (9) ◽  
pp. 853-861 ◽  
Author(s):  
Sergey Strelkov ◽  
Mirko von Elstermann ◽  
Dietmar Schomburg
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Corynebacterium Glutamicum ◽  
Growth Conditions ◽  
Gas Chromatography Mass Spectrometry ◽  
Fast Method ◽  
Chromatography Mass Spectrometry ◽  
High Matrix ◽  
First Time ◽  
Derivatives Of

AbstractAn analytical method based on gas chromatography/mass spectrometry was developed for metabolome investigation ofCorynebacterium glutamicum. For the first time a fast method for metabolic screening that can be automated is described for this organism. More than 1000 compounds could be detected per experiment, ca. 330 of those showed a peak area significantly above background. Out of these 164 compounds were identified so far, representing derivatives of 121 different metabolites, which were quantified in one sample. In spite of the different chemical nature of metabolites and high matrix content, a measurement reproducibility in the range of 6% error was achieved. The application of this method for the analysis of the adaptation ofC. glutamicumto different growth conditions is demonstrated.

scholarly journals Metabolic phenotyping of a model of adipocyte differentiation

2009 ◽  
Vol 39 (2) ◽  
pp. 109-119 ◽  
Author(s):  
Lee D. Roberts ◽  
Sam Virtue ◽  
Antonio Vidal-Puig ◽  
Andrew W. Nicholls ◽  
Julian L. Griffin
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acid ◽  
Cell Line ◽  
Tca Cycle ◽  
Acid Synthesis ◽  
Metabolic Phenotype ◽  
Gas Chromatography Mass Spectrometry ◽  
Resonance Spectroscopy ◽  
Polyamine Biosynthesis ◽  
Chromatography Mass Spectrometry

The 3T3-L1 murine cell line is a robust and widely used model for the study of adipogenesis and processes occurring in mature adipocytes. The fibroblastic like cells can be induced by hormones to differentiate into mature adipocytes. In this study, the metabolic phenotype associated with differentiation of the 3T3-L1 cell line has been studied using gas chromatography-mass spectrometry, 1H nuclear magnetic resonance spectroscopy, liquid chromatography-mass spectrometry, direct infusion-mass spectrometry, and 13C substrate labeling in conjunction with multivariate statistics. The changes in metabolite concentrations at distinct periods during differentiation have been defined including alterations in the TCA cycle, glycolysis, the production of odd chain fatty acids by α-oxidation, fatty acid synthesis, fatty acid desaturation, polyamine biosynthesis, and trans-esterification to produce complex lipids. The metabolic changes induced during differentiation of the 3T3-L1 cell line were then compared with the metabolic differences between pre- and postdifferentiation primary adipocytes. These metabolic alterations reflect the changing role of the 3T3-L1 cells during differentiation, as well as possibly providing metabolic triggers to stimulate the processes which occur during differentiation.

Sign in / Sign up

Export Citation Format

Share Document