scholarly journals Metabolic phenotyping of a model of adipocyte differentiation

2009 ◽  
Vol 39 (2) ◽  
pp. 109-119 ◽  
Author(s):  
Lee D. Roberts ◽  
Sam Virtue ◽  
Antonio Vidal-Puig ◽  
Andrew W. Nicholls ◽  
Julian L. Griffin
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acid ◽  
Cell Line ◽  
Tca Cycle ◽  
Acid Synthesis ◽  
Metabolic Phenotype ◽  
Gas Chromatography Mass Spectrometry ◽  
Resonance Spectroscopy ◽  
Polyamine Biosynthesis ◽  
Chromatography Mass Spectrometry

The 3T3-L1 murine cell line is a robust and widely used model for the study of adipogenesis and processes occurring in mature adipocytes. The fibroblastic like cells can be induced by hormones to differentiate into mature adipocytes. In this study, the metabolic phenotype associated with differentiation of the 3T3-L1 cell line has been studied using gas chromatography-mass spectrometry, 1H nuclear magnetic resonance spectroscopy, liquid chromatography-mass spectrometry, direct infusion-mass spectrometry, and 13C substrate labeling in conjunction with multivariate statistics. The changes in metabolite concentrations at distinct periods during differentiation have been defined including alterations in the TCA cycle, glycolysis, the production of odd chain fatty acids by α-oxidation, fatty acid synthesis, fatty acid desaturation, polyamine biosynthesis, and trans-esterification to produce complex lipids. The metabolic changes induced during differentiation of the 3T3-L1 cell line were then compared with the metabolic differences between pre- and postdifferentiation primary adipocytes. These metabolic alterations reflect the changing role of the 3T3-L1 cells during differentiation, as well as possibly providing metabolic triggers to stimulate the processes which occur during differentiation.

scholarly journals DIFFERENTIATION OF FATTY ACID COMPOSITION OF BUTTER ADULTERATED WITH LARD USING GAS CHROMATOGRAPHY MASS SPECTROMETRY COMBINED WITH PRINCIPAL COMPONENET ANALYSIS

2016 ◽  
Vol 78 (2) ◽  
Author(s):  
Nurrulhidayah Ahmad Fadzlillah ◽  
Abdul Rohman ◽  
Arieff Salleh Rosman ◽  
Farahwahida Mohd Yusof ◽  
Amin Ismail ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Principal Component Analysis ◽  
Gas Chromatography ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Principal Component ◽  
Food Samples ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Halal Authentication

Butter is high priced product; as a consequence, butter can be subjected for adulteration with low price components such as lard. The presence of lard in any products is not allowed for Muslim and Jewish, therefore, its presence must be identified. Gas chromatography-mass spectrometry was successfully used to detect and discriminate butter from adulterated with lard. Results were presented in the form of chromatogram. Principal component analysis (PCA) was used to interpret the data and provided a good grouping of samples with 55.8% of the variation accounted for by PC 1 and 21.5% were accounted for by PC 2. All the lard containing samples formed a separate group from the samples that were free of lard. This method can be developed into a rapid method for detecting the presence of lard in food samples for Halal authentication.

scholarly journals EVALUATION OF GAS CHROMATOGRAPHY-MASS SPECTROMETRY AND CYTOTOXICITY OF ETHANOLIC LEAF EXTRACT OF ACACIA CAESIA (L.) WILLD. AGAINST HELA CELL LINE

2019 ◽  
Vol 12 (1) ◽  
pp. 520
Author(s):  
Sharmila S ◽  
Ramya E K
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Leaf Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Wide Range ◽  
Ethanolic Leaf Extract

Objective: The objective of this study is to analyze the bioactive compounds of the ethanolic leaf extract of Acacia caesia using gas chromatography-mass spectrometry (GC-MS) method and also screen the in vitro cytotoxic activity against HeLa-E 72 cancer cell line.Methods: The present research was carried out using GC-MS analysis, while mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology and Wiley library. Cytotoxicity was assessed with 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide assay, and cellular morphological alterations were studied using phase contrast inverted light microscope of 400×. The ethanol extract of A. caesia was screened for their cytotoxicity at different concentrations (12.5–200 μg/ml), to determine the mean percentage (%) cell viability.Results: The results of GC/MS analysis showed the presence of 41 major compounds. In terms of percentage amounts, 1,8-diphenyl-3,4,10,11- tetrahydro[1,4]dioxino[2,3-g:5,6-g’]diisoquinoline, 6-(chloromethyl)-4-(3,4-dimethoxy-2-(phenylmethoxy)-phenyl)-3-methyl-2-yridinecarboxylate, and 2’,4’,6’-Trinitro-5’-phenyl-1,1’:3’,1”-terphenyl were predominant in the extract and have the property of antioxidant, antidepressant potential, antibacterial activity, cytotoxic, diabetic, and induced brain activity. The results of cytotoxicity at highest concentration (200 μg/ml) of the cells became rounder, shrunken and showed signs of detachment from the surface of the wells denoting cell death.Conclusions: From this study, it is obvious that A. caesia leaf extracts contain various bioactive constituents with a wide range of medicinal properties which is used to treat multiple disorders and it also gives a detailed insight about the phytochemical profile which could be exploited for the development of plant-based drugs. Further, the ethanolic extract of A. caesia exhibits potent cytotoxic activity against HeLa-E 72 cell line.

scholarly journals Ultra-performance liquid chromatography-mass spectrometry for precise fatty acid profiling of oilseed crops

2019 ◽  
Author(s):  
Alina Chernova ◽  
Pavel Mazin ◽  
Svetlana Goryunova ◽  
Denis Goryunov ◽  
Yakov Demurin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Ultra Performance Liquid Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Liquid Chromatography Mass Spectrometry ◽  
Oilseed Crops ◽  
Oil Crops ◽  
Chromatography Mass Spectrometry

Oilseed crops are one of the most important sources of vegetable oils for food and industry. Nutritional and technical properties of vegetable oil are primarily determined by its fatty acid (FA) composition. The content and composition of FAs in plants are commonly determined using gas chromatography-mass spectrometry (GS-MS) or gas chromatography-flame ionization detection (GC-FID) techniques. In the present work, we applied ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) technique to FA profiling of sunflower and rapeseed seeds and compared this method with the GC-FID technique. GC-FID detected 11 FAs in sunflower and 13 FAs in rapeseed, UPLC-MS appeared to be more sensitive, detecting about 2.5 times higher number of FAs in both plants. In addition to even-chain FAs, UPLC-MS was able to detect odd-chain Fas. The longest FA detected using GC-FID was a FA with 24 carbon atoms, whereas UPLC-MS could reveal the presence of longer FAs with the tails of up to 28 carbon atoms. Based on our results, we may conclude that UPLC-MS has a great potential to be used for the assessment of the FA profile of oil crops.

Synthesis of Microcapsule by Staphylococcus aureus Is Not Responsive to Environmental Phosphate Concentrations

1998 ◽  
Vol 66 (8) ◽  
pp. 4004-4007 ◽  
Author(s):  
Karen F. Fox ◽  
George C. Stewart ◽  
Alvin Fox
Keyword(s):  
Mass Spectrometry ◽  
Staphylococcus Aureus ◽  
Teichoic Acid ◽  
Acid Synthesis ◽  
Growth Conditions ◽  
Phosphate Concentration ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Teichuronic Acid ◽  
Environmental Component

ABSTRACT The polysaccharide microcapsule of Staphylococcus aureus has been reported to be differentially expressed depending on growth conditions, with phosphate concentration being the critical environmental component. This study evaluated the effect of growth of a serotype 8 strain of S. aureus in phosphate-replete and phosphate-limiting media on microcapsule production. The presence of the cell wall polymers microcapsule and teichoic acid was measured by both gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry. Production of microcapsule was unaffected by changes in the environmental phosphate concentration. There was, additionally, no evidence for a shift from teichoic acid to teichuronic acid synthesis.

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