scholarly journals Isolation of Enterococcus faecalis Clinical Isolates That Efficiently Adhere to Human Bladder Carcinoma T24 Cells and Inhibition of Adhesion by Fibronectin and Trypsin Treatment

1999 ◽  
Vol 67 (4) ◽  
pp. 1585-1592 ◽  
Author(s):  
Akihiko Shiono ◽  
Yasuyoshi Ike
Keyword(s):  
Electron Microscopy ◽  
Enterococcus Faecalis ◽  
Specific Protein ◽  
Cell Surfaces ◽  
Trypsin Treatment ◽  
Human Bladder ◽  
T24 Cells ◽  
Different Strains ◽  
Tract Infections ◽  
Scanning Electron

ABSTRACT The adherence of Enterococcus faecalis strains to human T24 cells was examined by scanning electron microscopy. Five highly adhesive strains were identified from 30 strains isolated from the urine of patients with urinary tract infections. No efficiently adhesive strains were found among the 30 strains isolated from the feces of healthy students. The five isolated strains also adhered efficiently to human bladder epithelial cells. Analysis of restriction endonuclease-digested plasmid DNAs and chromosome DNAs showed that the five strains were different strains isolated from different patients. The adhesiveness of these strains was inhibited by treatment with fibronectin or trypsin, implying that a specific protein (adhesin) on the bacterial cell surface mediates adherence to fibronectin on the host cell surfaces, and the adhesin differs from the reported adhesins.

Preservation of Plant Cell Surfaces For Scanning Electron Microscopy

1973 ◽  
Vol 31 ◽  
pp. 472-473
Author(s):  
Linda M. Sicko ◽  
Thomas E. Jensen
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Critical Point ◽  
Filter Paper ◽  
Freeze Drying ◽  
Cell Surfaces ◽  
Air Drying ◽  
Popular Method ◽  
Critical Point Drying ◽  
Scanning Electron

The use of critical point drying is rapidly becoming a popular method of preparing biological samples for scanning electron microscopy. The procedure is rapid, and produces consistent results with a variety of samples. The preservation of surface details is much greater than that of air drying, and the procedure is less complicated than that of freeze drying. This paper will present results comparing conventional air-drying of plant specimens to critical point drying, both of fixed and unfixed material. The preservation of delicate structures which are easily damaged in processing and the use of filter paper as a vehicle for drying will be discussed.

Scanning electron microscopy of the duodenal mucosa of lambs infected with Trichostrongylus colubriformis

Parasitology ◽  
1973 ◽  
Vol 67 (3) ◽  
pp. 307-314 ◽  
Author(s):  
I. K. Barker
Keyword(s):  
Electron Microscopy ◽  
Inflammatory Cells ◽  
Goblet Cells ◽  
Duodenal Mucosa ◽  
Regular Pattern ◽  
Cell Surfaces ◽  
Trichostrongylus Colubriformis ◽  
Thin Walled ◽  
Micro Organisms ◽  
Scanning Electron

Duodenal mucosae of uninfected lambs and lambs inoculated at least 16 days earlier with 85000–140000 Trichostrongylus colubriformis larvae were examined with the scanning electron microscope. Normal duodenum had tall spatulate villi with surface folds upon which goblet cells and a regular pattern of hexagonal enterocytes were seen. Micro villi on normal enterocytes were closely packed and imparted a granular surface texture. In heavily infected areas of gut the villi were atrophic, the mucosa sometimes being composed of irregular masses and ridges, with crypt mouths, often surrounded by collars of cells, opening into the surface. More severely affected mucosae were flat, with protuberant collars of cells surrounding crypt mouths. There were rounded bodies, interpreted as sloughing enterocytes, or inflammatory cells, on the mucosal surface. Apices of enterocytes were domed and microvilli were sparse and irregular. Micro-organisms were numerous on cell surfaces. Nematodes were located in sinuous thin-walled tunnels in the epithelium. The mucosal microtopography is compared with that of coeliac disease of humans, nippostrongylosis in rats and with villus atrophy in pigs.

Cellular projections seen by Scanning Electron Microscopy

1987 ◽  
Vol 45 ◽  
pp. 972-973
Author(s):  
Veronika Burmeister ◽  
Paul D. Millikin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Uranyl Acetate ◽  
Resolving Power ◽  
Pathological State ◽  
Depth Of Focus ◽  
Cell Surfaces ◽  
Transmission Electron ◽  
Ideal Tool ◽  
Scanning Electron

Scanning electron microscopy is an ideal tool for the visualization of projections in biological cell surfaces because it combines high resolving power with extraordinary depth of focus. To appreciate the inside structures of cellular projections transmission electron microscopy is ideal, since it enables the identification of intricate ultrastructures In this presentation we compare the size and ultrastructure of microvilli in a normal as well as pathological state in mesothelium, cilia of the nasal mucosa and pseudoprojections of spirochetes.Specimens were routinely processed: fixed in 2.5% Glutaraldehyde, rinsed in Millonig's Phosphate Buffer and carried through Ethanol to 100%; SEM specimens were then critical point dried and gold-coated. TEM specimens were put into Propylene Oxide and subsequently polymerized in Epon 812. Silversections were cut and stained in Uranyl Acetate and Lead Citrate. JEOL, JEM 100 C transmission and JSM 35 scanning microscopes were used.

Effect of piliation on Klebsiella pneumoniae infection in rat bladders

1980 ◽  
Vol 30 (2) ◽  
pp. 554-561
Author(s):  
R C Fader ◽  
C P Davis
Keyword(s):  
Electron Microscopy ◽  
Urinary Tract ◽  
Klebsiella Pneumoniae ◽  
Mammalian Cells ◽  
Parent Strain ◽  
Single Parent ◽  
Integral Role ◽  
Tract Infections ◽  
Scanning Electron

The possible role of pili in the pathogenesis of urinary tract infection caused by Klebsiella pneumoniae was investigated in a rat model of cystitis by utilizing piliated- and nonpiliated-phase organisms derived from a single parent strain. Bladder surfaces were examined for evidence of infection by scanning electron microscopy. In animals infected with piliated-phase organisms, foci of infection were evident in the majority of bladders examined. Rat bladders associated with nonpiliated-phase bacteria showed little evidence of infection. The ability of methyl-D-mannoside, a known inhibitor of pilus-mediated adherence to mammalian cells, to protect the bladder surface from colonization was also tested. The results showed a significant decrease in the ability of piliated-phase K. pneumoniae to establish infection in bladders. These observations suggest that pili may play an integral role in the ability of K. pneumoniae to cause urinary tract infections by mediating the attachment of the bacteria to the uroepithelial surface.

Effect of Cooling Stimulus on Collection Efficiency of Calf Chondrocytes Cultivated on Metal Surface

2017 ◽  
Vol 11 (6) ◽  
pp. 925-931 ◽  
Author(s):  
Yuta Kurashina ◽  
◽  
Shogo Miyata ◽  
Jun Komotori
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Culture ◽  
Cell Proliferation ◽  
Collection Efficiency ◽  
Trypsin Treatment ◽  
Culture Substrate ◽  
A Cell ◽  
Number Of Cells ◽  
Scanning Electron

A cell culture module capable of cooling stimulus to collect cells efficiently on a metal culture substrate was developed. We evaluated the cell collection ratio and morphology of the collected cells. Following a cooling stimulus (0°C) for 20 min, the number of collected cells was increased by 50% compared to that collected after trypsin treatment without pipetting from the metal culture substrate. Following the cooling stimulus, cells were observed by fluorescence microscopy and scanning electron microscopy; the cell filopodia were shrunken compared to non-cooling-stimulated cells. Furthermore, the combination of collagenase and cooling stimulation resulted in the collection of a comparable number of cells as that obtained using only trypsin. Thus, cell proliferation was improved compared to that following trypsin treatment. Therefore, this method can be applied for culturing cells that are susceptible to trypsin damage.

AGGREGATION TO COLONIES OF THE GREEN ALGA PEDIASTRUM: EXPERIMENTS AND SIMULATIONS

2000 ◽  
Vol 08 (04) ◽  
pp. 373-398
Author(s):  
MARC GREWE ◽  
MARIO MARKUS
Keyword(s):  
Electron Microscopy ◽  
Green Alga ◽  
Cell Orientation ◽  
Cell Surfaces ◽  
Cell Numbers ◽  
Video Recordings ◽  
Cell Shapes ◽  
Natural Aggregates ◽  
Scanning Electron ◽  
Cell Cell

We simulate the aggregation of zoospores of the green alga Pediastrum simplex into a planar colony — including radially outward cell orientation. The model assumptions are: cells are randomly kicked by flagella, an ellipsoidal boundary repells cells and flagella, overlapping cell surfaces lead to dissipative cell-cell repulsion, cell-cell attractive forces occur around arches close to the cell's surfaces, there is friction of the cells with the medium; the process ends by an extension of the attractive arches followed by the stop of flagellar propulsion and finally a change of cell shapes from spherical to nearly triangular with contact-inhibited horn-like extensions. These assumptions are directly observed or indirectly inferred from the literature, light microscopy (video recordings) and scanning electron microscopy. We perform calculations with cell numbers ranging from 4 to 64. Additional simulations permit to discard alternative models, including planarization via cell-cell attraction only, chemotaxis or cohesive intercellular gliding. Our model yields patterns agreeing well with symmetrical as well as with disordered natural aggregates.

scholarly journals A Comparison of Er:YAG Laser with Photon-Initiated Photoacoustic Streaming, Nd:YAG Laser, and Conventional Irrigation on the Eradication of Root Dentinal Tubule Infection by Enterococcus faecalis Biofilms: A Scanning Electron Microscopy Study

Scanning ◽  
2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Burcu Ozses Ozkaya ◽  
Kamran Gulsahi ◽  
Mete Ungor ◽  
Julide Sedef Gocmen
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Enterococcus Faecalis ◽  
Nd:Yag Laser ◽  
Er:Yag Laser ◽  
Dentinal Tubule ◽  
Sem Images ◽  
Apical Position ◽  
Conventional Irrigation ◽  
Scanning Electron

This study evaluated the antimicrobial efficacy of Er:YAG laser activation with photon-initiated photoacoustic streaming (PIPS), Nd:YAG laser disinfection, and conventional irrigation on Enterococcus faecalis biofilms using scanning electron microscopy (SEM). Biofilms were grown on 110 root halves and divided into the following: Groups 1 and 2 (saline and 1% NaOCl with apical position of PIPS, resp.), Groups 3 and 4 (saline and 1% NaOCl with coronal position of PIPS, resp.), Groups 5 and 6 (Nd:YAG laser after saline and 1% NaOCl irrigation, resp.) and Groups 7, 8, and 9 (conventional irrigation with 1% NaOCl, 6% NaOCl, and saline, resp.). SEM images of the apical, middle, and coronal levels were examined using a scoring system. Score differences between Groups 1 and 2 were insignificant at all levels in the remaining biofilm. Group 4 had significantly greater bacterial elimination than Group 3 at all levels. Differences in Nd:YAG laser irradiation between Groups 5 and 6 were insignificant. Groups 7 and 8 were insignificantly different, except at the middle level. Saline group had a higher percentage of biofilms than the others. In this study, PIPS activation with NaOCl eliminates more E. faecalis biofilms in all root canals regardless of the position of the fiber tip.

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