Scanning electron microscopy of the duodenal mucosa of lambs infected with Trichostrongylus colubriformis

Parasitology ◽  
1973 ◽  
Vol 67 (3) ◽  
pp. 307-314 ◽  
Author(s):  
I. K. Barker
Keyword(s):  
Electron Microscopy ◽  
Inflammatory Cells ◽  
Goblet Cells ◽  
Duodenal Mucosa ◽  
Regular Pattern ◽  
Cell Surfaces ◽  
Trichostrongylus Colubriformis ◽  
Thin Walled ◽  
Micro Organisms ◽  
Scanning Electron

Duodenal mucosae of uninfected lambs and lambs inoculated at least 16 days earlier with 85000–140000 Trichostrongylus colubriformis larvae were examined with the scanning electron microscope. Normal duodenum had tall spatulate villi with surface folds upon which goblet cells and a regular pattern of hexagonal enterocytes were seen. Micro villi on normal enterocytes were closely packed and imparted a granular surface texture. In heavily infected areas of gut the villi were atrophic, the mucosa sometimes being composed of irregular masses and ridges, with crypt mouths, often surrounded by collars of cells, opening into the surface. More severely affected mucosae were flat, with protuberant collars of cells surrounding crypt mouths. There were rounded bodies, interpreted as sloughing enterocytes, or inflammatory cells, on the mucosal surface. Apices of enterocytes were domed and microvilli were sparse and irregular. Micro-organisms were numerous on cell surfaces. Nematodes were located in sinuous thin-walled tunnels in the epithelium. The mucosal microtopography is compared with that of coeliac disease of humans, nippostrongylosis in rats and with villus atrophy in pigs.

Nematodirus battus: scanning electron microscope studies of the duodenal mucosa of infected lambs

Parasitology ◽  
1980 ◽  
Vol 81 (3) ◽  
pp. 573-578 ◽  
Author(s):  
Jean Martin ◽  
D. L. Lee
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Goblet Cells ◽  
Duodenal Mucosa ◽  
Intestinal Damage ◽  
Third Stage ◽  
Scanning Electron

SUMMARYLambs given 60000 third-stage larvae of Nematodirus battus were killed on days 16, 20, 22, 24 and 32 of the infection and the duodenal mucosa was examined by scanning electron microscopy (SEM). On day 16 of the infection the villi were long and finger-like and, although goblet cells were visible, the surface of the villi was not extensively folded, as is the surface of villi of uninfected control animals. By day 20 of the infection the villi had been reduced to flattened, plate-like structures or had formed low, irregular-shaped ridges. On this day of the infection the nematodes appeared to be enclosed by mucus-like material. As the infection progressed the intestinal damage was repaired, so that, by day 24 of the infection, the surface of the intestine was covered by short, smooth, finger-like projections and, by day 32 of the infection, the morphology of the intestine was somewhat similar to that of the control lamb. The possible role of villus atrophy and of mucus in the rejection of N. battus from the intestine is discussed.

Preservation of Plant Cell Surfaces For Scanning Electron Microscopy

1973 ◽  
Vol 31 ◽  
pp. 472-473
Author(s):  
Linda M. Sicko ◽  
Thomas E. Jensen
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Critical Point ◽  
Filter Paper ◽  
Freeze Drying ◽  
Cell Surfaces ◽  
Air Drying ◽  
Popular Method ◽  
Critical Point Drying ◽  
Scanning Electron

The use of critical point drying is rapidly becoming a popular method of preparing biological samples for scanning electron microscopy. The procedure is rapid, and produces consistent results with a variety of samples. The preservation of surface details is much greater than that of air drying, and the procedure is less complicated than that of freeze drying. This paper will present results comparing conventional air-drying of plant specimens to critical point drying, both of fixed and unfixed material. The preservation of delicate structures which are easily damaged in processing and the use of filter paper as a vehicle for drying will be discussed.

Scanning electron microscopy of the operculum of Garra lamta (Hamilton) (Cyprinidae:Cypriniformes), an Indian hill stream fish

2010 ◽  
Vol 58 (3) ◽  
pp. 182 ◽  
Author(s):  
Swati Mittal ◽  
Usha Kumari ◽  
Pinky Tripathi ◽  
Ajay Kumar Mittal
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Epithelial Cells ◽  
Goblet Cells ◽  
Stream Fish ◽  
Male And Female ◽  
Fish Breeding ◽  
Inner Surface ◽  
Garra Lamta ◽  
Scanning Electron

The surface architecture of the epidermis on the outer surface of the operculum (OE) and the epithelium on the inner surface of the operculum (EISO) of Garra lamta was examined by scanning electron microscopy. The surface appeared smooth on the OE and wavy on the EISO. A wavy epithelium is considered to facilitate an increase in its stretchability, during the expansion of the branchial chamber. The OE and the EISO were covered by a mosaic pavement of epithelial cells with characteristic patterns of microridges and microbridges. Interspersed between the epithelial cells were mucous goblet cell pores, which were not significantly different in number in the OE and the EISO. Nevertheless, their surface area in the EISO was significantly higher than in the OE. This could be an adaptation to secrete higher amounts of mucus on the EISO for keeping the branchial chamber lining clean, avoiding clogging, the increased slipperiness reducing friction from water flow and increased efficiency in protecting against microbial attachments. Rounded bulges on the OE and the EISO were associated with mucous goblet cells. The absence of the taste buds in the EISO, in contrast to the OE, suggests that their function in the branchial chamber may not be of much significance in this fish. Breeding tubercles on the OE are believed to facilitate better contact between the male and female during breeding.

scholarly journals Characterization of monoclonal IgG cryoglobulins: fine-structural and morphological analysis

Blood ◽  
1987 ◽  
Vol 69 (2) ◽  
pp. 677-681 ◽  
Author(s):  
DN Podell ◽  
CH Packman ◽  
J Maniloff ◽  
GN Abraham
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Morphological Analysis ◽  
Vascular Occlusion ◽  
Molecular Clusters ◽  
Morphological Properties ◽  
Transmission Electron ◽  
Thin Walled ◽  
Scanning Electron

Abstract The morphology of the amorphous, gelatinous, and crystalline varieties of monoclonal IgG cryoglobulins was analyzed by light and transmission and scanning electron microscopy. Each cryoglobulin had a characteristic fine structure that correlated with its gross morphology. Transmission electron microscopy showed that the amorphous precipitates were random and disorganized molecular clumps. In contrast, cryogels were thin-walled, well-organized, and hydrated strawlike clusters, whereas cryocrystals formed tightly compacted, highly structured molecular clusters. Crystals that formed in blood produced rouleaux, and analysis by scanning electron microscopy indicated that the crystals could form thick-walled, branching, macromolecular nets that could physically trap cells. The morphological properties provided visual impressions by which cryoglobulins could cause clinical disease secondary to vascular occlusion produced by self- associated IgG cryoglobulin molecules.

Study of the nasopharynx in man by scanning electron microscopy

1988 ◽  
Vol 102 (12) ◽  
pp. 1102-1106 ◽  
Author(s):  
M. Gulisano ◽  
G. Polli ◽  
G. Biondi ◽  
P. Pacini
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Healthy Volunteers ◽  
Squamous Epithelium ◽  
Goblet Cells ◽  
Transitional Epithelium ◽  
Ciliated Cells ◽  
Scanning Electron

AbstractTo date only few and often disagreeing studies about human nasopharynx are available.The present research has the purpose to give a contribution to the knowledge of nasopharyngeal epithelium using S.E.M The study was carried out on biopsies taken from 20 healthy volunteers.The surface of nasopharynx is covered by ciliated cells, microvilli provided cells and goblet cells.Areas covered with squamous epithelium and presumably transitional epithelium were observed.The possibility that ciliated become microvilli provided cells is discussed.

Morphological Aspects of the Traumatic Chronic Subdural Hematoma Capsule: SEM Studies

2007 ◽  
Vol 13 (3) ◽  
pp. 211-219 ◽  
Author(s):  
Marek Moskała ◽  
Igor Gościński ◽  
Józef Kałuża ◽  
Jarosław Polak ◽  
Mariusz Krupa ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Vessels ◽  
Morphological Changes ◽  
Chronic Subdural Hematoma ◽  
Dynamic Changes ◽  
Inflammatory Reactions ◽  
Morphological Aspects ◽  
Thin Walled ◽  
Scanning Electron

The morphology of the outer and inner membranes of traumatic chronic subdural hematomas (CSDHs) surgically removed from eight patients was investigated by scanning electron microscopy (SEM). Hematomas were divided into three groups based on time that had passed from the initiation of trauma to surgery. Structure of the CSDHs showed gradual morphological changes of the developing hematoma capsule. They initially included angiogenic and aseptic inflammatory reactions followed by progressive involvement of fibroblasts—proliferating and producing collagen fibrils. Numerous capillaries suggesting formation of new blood vessels were observed mainly in young hematomas removed between 15 and 21 days after trauma. In “older” hematomas (40 days after trauma), more numerous capillaries and thin-walled sinusoids were accompanied by patent, larger diameter blood vessels. Within the fibrotic outer membrane of the “oldest” hematoma capsules (60 or more days after trauma), especially in the area over the hematoma cavity, blood vessels were frequently occluded by clots. The results suggest dynamic changes in cellular and vascular organization of traumatic CSDH capsules paralleling the progression in hematoma age.

scholarly journals Isolation of Enterococcus faecalis Clinical Isolates That Efficiently Adhere to Human Bladder Carcinoma T24 Cells and Inhibition of Adhesion by Fibronectin and Trypsin Treatment

1999 ◽  
Vol 67 (4) ◽  
pp. 1585-1592 ◽  
Author(s):  
Akihiko Shiono ◽  
Yasuyoshi Ike
Keyword(s):  
Electron Microscopy ◽  
Enterococcus Faecalis ◽  
Specific Protein ◽  
Cell Surfaces ◽  
Trypsin Treatment ◽  
Human Bladder ◽  
T24 Cells ◽  
Different Strains ◽  
Tract Infections ◽  
Scanning Electron

ABSTRACT The adherence of Enterococcus faecalis strains to human T24 cells was examined by scanning electron microscopy. Five highly adhesive strains were identified from 30 strains isolated from the urine of patients with urinary tract infections. No efficiently adhesive strains were found among the 30 strains isolated from the feces of healthy students. The five isolated strains also adhered efficiently to human bladder epithelial cells. Analysis of restriction endonuclease-digested plasmid DNAs and chromosome DNAs showed that the five strains were different strains isolated from different patients. The adhesiveness of these strains was inhibited by treatment with fibronectin or trypsin, implying that a specific protein (adhesin) on the bacterial cell surface mediates adherence to fibronectin on the host cell surfaces, and the adhesin differs from the reported adhesins.

Cellular projections seen by Scanning Electron Microscopy

1987 ◽  
Vol 45 ◽  
pp. 972-973
Author(s):  
Veronika Burmeister ◽  
Paul D. Millikin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Uranyl Acetate ◽  
Resolving Power ◽  
Pathological State ◽  
Depth Of Focus ◽  
Cell Surfaces ◽  
Transmission Electron ◽  
Ideal Tool ◽  
Scanning Electron

Scanning electron microscopy is an ideal tool for the visualization of projections in biological cell surfaces because it combines high resolving power with extraordinary depth of focus. To appreciate the inside structures of cellular projections transmission electron microscopy is ideal, since it enables the identification of intricate ultrastructures In this presentation we compare the size and ultrastructure of microvilli in a normal as well as pathological state in mesothelium, cilia of the nasal mucosa and pseudoprojections of spirochetes.Specimens were routinely processed: fixed in 2.5% Glutaraldehyde, rinsed in Millonig's Phosphate Buffer and carried through Ethanol to 100%; SEM specimens were then critical point dried and gold-coated. TEM specimens were put into Propylene Oxide and subsequently polymerized in Epon 812. Silversections were cut and stained in Uranyl Acetate and Lead Citrate. JEOL, JEM 100 C transmission and JSM 35 scanning microscopes were used.

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