scholarly journals Phylogenetic Analysis of Feline Immunodeficiency Virus in Feral and Companion Domestic Cats of New Zealand

2006 ◽  
Vol 81 (6) ◽  
pp. 2999-3004 ◽  
Author(s):  
Jessica J. Hayward ◽  
John Taylor ◽  
Allen G. Rodrigo
Keyword(s):  
Phylogenetic Analysis ◽  
New Zealand ◽  
Feline Immunodeficiency Virus ◽  
Nested Pcr ◽  
Phylogenetic Analyses ◽  
Domestic Cats ◽  
Intersubtype Recombination ◽  
Immunodeficiency Virus

ABSTRACT Nested PCR was used to amplify envelope V3-V6 gene fragments of feline immunodeficiency virus (FIV) from New Zealand cats. Phylogenetic analyses established that subtypes A and C predominate among New Zealand cats, with clear evidence of intersubtype recombination. In addition, 17 sequences were identified that were distinct from all known FIV clades, and we tentatively suggest these belong to a novel subtype.

Feline immunodeficiency virus subtypes in domestic cats in New Zealand

2007 ◽  
Vol 55 (6) ◽  
pp. 358-360 ◽  
Author(s):  
RKC Kann ◽  
JM Seddon ◽  
J Meers ◽  
RJG Zwijnenberg
Keyword(s):  
New Zealand ◽  
Feline Immunodeficiency Virus ◽  
Domestic Cats ◽  
Immunodeficiency Virus

scholarly journals Phylogenetic analysis to define feline immunodeficiency virus subtypes in 31 domestic cats in South Africa

2006 ◽  
Vol 77 (3) ◽  
Author(s):  
R. Kann ◽  
J. Seddon ◽  
M. Kyaw-Tanner ◽  
J.P. Schoeman ◽  
T. Schoeman ◽  
...  
Keyword(s):  
South Africa ◽  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Feline Immunodeficiency Virus ◽  
Envelope Gene ◽  
Domestic Cats ◽  
Blood Samples ◽  
Immunodeficiency Virus ◽  
The World ◽  
Subtype A

Feline immunodeficiency virus (FIV), a lentivirus, is an important pathogen of domestic cats around the world and has many similarities to human immunodeficiency virus (HIV). A characteristic of these lentiviruses is their extensive genetic diversity, which has been an obstacle in the development of successful vaccines. Of the FIV genes, the envelope gene is the most variable and sequence differences in a portion of this gene have been used to define 5 FIV subtypes (A, B, C, D and E). In this study, the proviral DNA sequence of the V3-V5 region of the envelope gene was determined in blood samples from 31 FIV positive cats from 4 different regions of South Africa. Phylogenetic analysis demonstrated the presence of both subtypes A and C, with subtype A predominating. These findings contribute to the understanding of the genetic diversity of FIV.

scholarly journals Prevalence of feline immunodeficiency virus and feline leukaemia virus in domestic cats in Hungary

2019 ◽  
Vol 5 (2) ◽  
pp. 205511691989209
Author(s):  
Anna Szilasi ◽  
Lilla Dénes ◽  
Eszter Krikó ◽  
Kristin Heenemann ◽  
Reinhard Ertl ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Statistical Analysis ◽  
Feline Immunodeficiency Virus ◽  
Domestic Cats ◽  
Gene Sequences ◽  
Leukaemia Virus ◽  
Apparent Prevalence ◽  
Subtype B ◽  
Immunodeficiency Virus ◽  
Feline Leukaemia Virus

Objectives Feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV) are retroviruses affecting cats worldwide. The objectives of the study were to estimate the prevalence of these retroviruses in domestic cats in Hungary and to characterise the phylogenetic relationships of FIV strains. Methods A total of 335 anticoagulated whole-blood samples obtained from both a healthy and ill cat population were examined for the presence of FIV and FeLV with two methods: ELISA and PCR. Statistical analysis was carried out to analyse the data obtained. Sequencing and phylogenetic analysis of partial polymerase ( pol) gene sequences was performed to describe circulating FIV subtypes. Results Statistical analysis showed 11.8% and 9.9% true prevalence of FeLV and FIV, respectively, with ELISA. The apparent prevalence calculated from the PCR results were 17.3% for FeLV and 13.1% for FIV. Phylogenetic analysis of partial pol gene sequences obtained from 22 FIV strains showed that all observed Hungarian strains belonged to FIV subtype B. The strains were grouped into several monophyletic subgroups reflecting the geographic locations of the origin of the samples. The overall mean genetic similarity between the analysed strains was 98.2%. Conclusions and relevance We report the first thorough overview of the prevalence of FeLV and FIV in Hungary, which is relatively high, and give insight into the genetic diversity of Hungarian strains of FIV.

scholarly journals Interspecies Transmission of Feline Immunodeficiency Virus from the Domestic Cat to the Tsushima Cat (Felis bengalensis euptilura) in the Wild

1999 ◽  
Vol 73 (9) ◽  
pp. 7916-7921 ◽  
Author(s):  
Yoshiaki Nishimura ◽  
Yuko Goto ◽  
Kumiko Yoneda ◽  
Yasuyuki Endo ◽  
Takuya Mizuno ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Cell Lines ◽  
Feline Immunodeficiency Virus ◽  
Lymphoid Cell ◽  
Domestic Cat ◽  
Interspecies Transmission ◽  
Domestic Cats ◽  
Immunodeficiency Virus ◽  
In The Wild ◽  
Lymphoid Cell Lines

ABSTRACT Feline immunodeficiency virus (FIV) was isolated from a wild-caught Tsushima cat (Felis bengalensis euptilura), an endangered Japanese nondomestic subspecies of leopard cat (F. bengalensis). Phylogenetic analysis of the env gene sequences indicated that the FIV from the Tsushima cat belonged to a cluster of subtype D FIVs from domestic cats. FIVs from both the Tsushima cat and the domestic cat showed similar levels of replication and cytopathicity in lymphoid cell lines derived from these two species. The results indicated the occurrence of interspecies transmission of FIV from the domestic cat to the Tsushima cat in the wild.

scholarly journals Quantification of Feline immunodeficiency virus (FIVpco) in peripheral blood mononuclear cells, lymph nodes and plasma of naturally infected cougars

2006 ◽  
Vol 87 (4) ◽  
pp. 967-975 ◽  
Author(s):  
David J. Blake ◽  
Jon Graham ◽  
Mary Poss
Keyword(s):  
Virus Replication ◽  
Peripheral Blood Mononuclear Cells ◽  
Feline Immunodeficiency Virus ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Domestic Cats ◽  
Peripheral Blood Mononuclear ◽  
Viral Loads ◽  
Immunodeficiency Virus ◽  
Blood Mononuclear Cells

Infection of domestic cats with Feline immunodeficiency virus (FIV) results in a fatal immunodeficiency disease, similar to Human immunodeficiency virus 1 (HIV-1) in humans. Elevated plasma viral loads in domestic cats are correlated to decreased survival time and disease progression. However, FIV is also maintained as an apathogenic infection in other members of the family Felidae including cougars, Puma concolor (FIVpco). It is not known whether the lack of disease in cougars is a result of diminished virus replication. A real-time PCR assay was developed to quantify both FIVpco proviral and plasma viral loads in naturally infected cougars. Proviral loads quantified from peripheral blood mononuclear cells (PBMC) ranged from 2·90×101 to 6·72×104 copies per 106 cells. Plasma viral loads ranged from 2·30×103 to 2·81×106 RNA copies ml−1. These data indicate that FIVpco viral loads are comparable to viral loads observed in endemic and epidemic lentivirus infections. Thus, the lack of disease in cougars is not due to low levels of virus replication. Moreover, significant differences observed among cougar PBMC proviral loads correlated to viral lineage and cougar age (P=0·014), which suggests that separate life strategies exist within FIVpco lineages. This is the first study to demonstrate that an interaction of lentivirus lineage and host age significantly effect proviral loads.

Feline Immunodeficiency Virus Depletes Naïve (CD45RA+) CD4+ Lymphocytes from the Blood and Lymph Nodes of Domestic Cats during Acute Pediatric Infection.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 3896-3896
Author(s):  
Calvin M. Johnson ◽  
Ayalew Mergia ◽  
Janelle Novak ◽  
Nazareth Gengozian
Keyword(s):  
T Lymphocytes ◽  
Lymph Nodes ◽  
Feline Immunodeficiency Virus ◽  
Pediatric Hiv ◽  
Lymphoid Tissues ◽  
Domestic Cats ◽  
Color Flow ◽  
Cd8 Cells ◽  
Immunodeficiency Virus ◽  
Cd4 Lymphocytes

Abstract Feline immunodeficiency virus (FIV) is an immunosuppressive lentivirus of domestic cats that serves as an animal model for the pathogenesis of CD4+ lymphopenia and thymus dysfunction in HIV infected humans. During most cases of adult and pediatric HIV infection, naïve CD4+ T lymphocytes recognized by the expression of the RA isoform of the leukocyte common antigen (CD45RA) are infected at a lower level than memory CD4+ T− lymphocytes; however, children with rapidly progressive disease due to thymic insufficiency harbor high levels of HIV within the CD45RA+ subpopulation. In FIV infected cats, the fate of naïve CD4 lymphocytes is unknown due to the lack of specific markers. Recently, a mAb (755) was reported to recognize the feline homologue to CD45RA, allowing the enumeration of naïve CD4 and CD8 lymphocytes in cats. The purpose of this study was to characterize the fate of CD4+CD45RA+ blood cells eight weeks after FIV infection. One-day-old kittens (n=6) were infected with virions either from a wild type clone (JSY3) or mutant ORF-A clone at equivalent reverse transcriptase units and compared to historical control data. Eight weeks after inoculation, the percentages of CD4+ and CD8+ cells belonging to the CD45RA+ subpopulation were measured by two-color flow cytometry. Both FIV inocula were associated with a reduction in total CD4+ lymphocytes from a median of 13% in controls to 8% in infected cats (P=0.004), contributing to a reduction in the CD4:CD8 ratio from 2.45 in controls to 0.76 in infected cats (P=0.007). The decline in CD4+ lymphocytes was attributable to a disproportionate loss of CD4+CD45RA+ cells: 69% of CD4+ cells were CD45RA+ in controls, as compared to 7% in FIV infected cats (P=0.004). In contrast, naïve CD8+ lymphocytes did not change significantly with FIV infection (67% of CD8+ cells were CD45RA+ in FIV infected cats as compared to 80% in controls). The distribution of CD45RA+ cells in the lymph nodes of FIV infected cats mirrored those in the blood. Together, these data suggest that acute FIV infection results in a rapid depletion of naïve CD4 lymphocytes throughout the blood and secondary lymphoid tissues, while proportions of naïve CD8 lymphocytes remain unchanged. CD4+CD45RA+ cells may be depleted during pediatric FIV infection through lytic infection or a transition to a memory phenotype lacking CD45RA.

Analysis of the genus Sunamphitoe Spence Bate, 1857 (Amphipoda: Ampithoidae) with descriptions of eight new species

Zootaxa ◽  
2017 ◽  
Vol 4269 (3) ◽  
pp. 301 ◽  
Author(s):  
RACHAEL A. PEART
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
New Zealand ◽  
Phylogenetic Analyses ◽  
Senior Synonym ◽  
The Status ◽  
Molecular Phylogenetic ◽  
Algal Host

Sunamphitoe Spence Bate, 1857 is one of several ampithoid amphipod genera to be reasonably specific to its algal host. Sunamphitoe was recently shown to be a senior synonym of Peramphithoe Conlan & Bousfield, 1982 by both morphological and molecular phylogenetic analyses that included selected exemplars of both putative genera. Phylogenetic analysis considering all species of Sunamphitoe herein corroborates the validity of this synonymy and also delineates several morphological groups within the genus. Eight new species are descibed herein, Sunamphitoe angrox sp. nov., S. batavia sp. nov., S. dampierensis sp. nov., S. jonathani sp. nov., S. lehae sp. nov., S. mixtura sp. nov., S. naturaliste sp. nov., and S. stevesmithi sp. nov., all from Australia and New Zealand; the status of the problematic New Zealand species, Sunamphitoe aorangi is resolved.

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