scholarly journals The tyrosine kinase substrate p120cas binds directly to E-cadherin but not to the adenomatous polyposis coli protein or alpha-catenin.

1995 ◽  
Vol 15 (9) ◽  
pp. 4819-4824 ◽  
Author(s):  
J M Daniel ◽  
A B Reynolds
Keyword(s):  
Cell Adhesion ◽  
Cell Line ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Beta Catenin ◽  
Kinase Substrate ◽  
Yeast Two Hybrid System ◽  
Two Hybrid ◽  
E Cadherin

The tyrosine kinase substrate p120cas (CAS), which is structurally similar to the cell adhesion proteins beta-catenin and plakoglobin, was recently shown to associate with the E-cadherin-catenin cell adhesion complex. beta-catenin, plakoglobin, and CAS all have an Arm domain that consists of 10 to 13 repeats of a 42-amino-acid motif originally described in the Drosophila Armadillo protein. To determine if the association of CAS with the cadherin cell adhesion machinery is similar to that of beta-catenin and plakoglobin, we examined the CAS-cadherin-catenin interactions in a number of cell lines and in the yeast two-hybrid system. In the prostate carcinoma cell line PC3, CAS associated normally with cadherin complexes despite the specific absence of alpha-catenin in these cells. However, in the colon carcinoma cell line SW480, which has negligible E-cadherin expression, CAS did not associate with beta-catenin, plakoglobin, or alpha-catenin, suggesting that E-cadherin is the protein which bridges CAS to the rest of the complex. In addition, CAS did not associate with the adenomatous polyposis coli (APC) tumor suppressor protein in any of the cell lines analyzed. Interestingly, expression of the various CAS isoforms was quite heterogeneous in these tumor cell lines, and in the colon carcinoma cell line HCT116, which expresses normal levels of E-cadherin and the catenins, the CAS1 isoforms were completely absent. By using the yeast two-hybrid system, we confirmed the direct interaction between CAS and E-cadherin and determined that CAS Arm repeats 1 to 10 are necessary and sufficient for this interaction. Hence, like beta-catenin and plakoglobin, CAS interacts directly with E-cadherin in vivo; however, unlike beta-catenin and plakoglobin, CAS does not interact with APC or alpha-catenin.

Expression of the cell–cell adhesion molecule E-cadherin in tongue carcinoma cell lines

1994 ◽  
Vol 108 (11) ◽  
pp. 957-961 ◽  
Author(s):  
A. R. Kinsella ◽  
G. L. Bowie ◽  
J. K. Fields ◽  
A. S. Jones
Keyword(s):  
Cell Adhesion ◽  
Cell Line ◽  
Cell Lines ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Carcinoma Cell ◽  
Tumour Progression ◽  
Tongue Carcinoma ◽  
Carcinoma Cell Lines ◽  
E Cadherin

AbstractA reduction in cell adhesiveness and cell invasion are essential steps in tumour progression. In the present study six tongue carcinoma cell lines were compared with regard to their invasive potential in two in vitro invasion assay systems and for their patterns of expression of the cell–cell adhesion molecule E-cadherin. The three cell lines negative for E-cadherin expression were invasive in both assays. One cell line with strong E-cadherin expression was strongly invasive and one weakly invasive. One cell line with reduced E-cadherin expression was weakly invasive. There was no significant pattern to these findings (x2 = 0.375; p = 0.54). This supports previous studies from this group that suggest that E-cadherin is only one of the presumably many molecules involved in tumour progression in squamous cell carcinoma of the tongue.

scholarly journals A clear cancer cell line (150057) derived from human endometrial carcinoma harbors two novel mutations

BMC Cancer ◽  
2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Yu-Hsun Chang ◽  
Dah-Ching Ding
Keyword(s):  
Endometrial Cancer ◽  
Cell Carcinoma ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Clear Cell ◽  
Carcinoma Cell ◽  
Clear Cell Carcinoma ◽  
Carcinoma Cell Line ◽  
Novel Mutations

Abstract Background Cell lines are extremely useful for both basic and clinical research. Thus, establishing endometrial cancer cell lines with malignant histology is important. This study aimed to extensively characterize an endometrial clear cell carcinoma cell line. Methods This cell line, named 150,057, was derived from the endometrial clear cell cancer of a 63-year-old woman. The morphology, chromosomes, chemosensitivity, tumor markers, xenotransplantation characteristics, and cancer-related genes of the cell line were characterized. Results This cell line exhibited adequate growth, being passaged more than 70 times. The morphology of the cells was polygonal with a cobblestone-like appearance. Karyotyping of the cell line revealed a hypodiploid chromosomal number. 150057 cells expressed CA19–9 and CA125. The cell line was sensitive to doxorubicin, paclitaxel, carboplatin, and cisplatin. After the cells were transplanted into the subcutaneous region of non-obese diabetic-severe combined immunodeficiency mice, they generated xenograft tumors with similar histology as the original tumor. A total of 59 somatic nucleotide mutations were identified in 25 of the 53 examined tumor suppressor genes and oncogenes. Two novel mutations were found in FGFR3 and ARID1A. Conclusion We established and characterized an endometrial clear cell carcinoma cell line that may be useful in carcinogenesis and treatment research for endometrial cancer.

scholarly journals ID: 1012 Cytotoxicity of Corchorus olitorius Linn. aqueous leaf extract against human carcinoma cells

2017 ◽  
Vol 4 (S) ◽  
pp. 86
Author(s):  
John Paul Tosoc
Keyword(s):  
Cell Viability ◽  
Cell Line ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Liver Carcinoma ◽  
Human Carcinoma ◽  
Carcinoma Cell Lines ◽  
Viability Percentage

C. olitorius are used as herbal medicine and eaten as vegetable by local people in Philippines.  The T’boli tribe commonly uses this plant to treat common illnesses such as pimples, wounds, boils, and inflammations. According to studies, the leaves of C. olitorius has properties to treat demulcent, diuretic, febrifuge, chronic cystitis, dysuria and even tumor. The aim of this study is to evaluate the cytotoxicity properties of the crude aqueous extract of C. olitorius leaf against colon (HCT116), breast (MCF-7), and liver (HepG2) carcinoma cell-lines using the MTT cell proliferation assay. The results showed that the aqueous extract has potential cytotoxicity activity against the three-carcinoma cell-lines. The aqueous extract was most bioactive in the colon carcinoma cell-line (HCT116) with cell viability percentage of 23.53 and 32.48 in 100 and 10 µg/mL, respectively. It was then followed by the breast carcinoma cell-line (MCF7) with cell viability percentage of 16.23 and 30.25 in 100 and 10 µg/mL, respectively. The extract was least but still bioactive in the liver carcinoma cell-line (HepG2) with cell viability percentage of 91.20 and 132.76 in 100 and 10 µg/mL, respectively. This study illustrates the importance of toxicological screening to confirm the safety and efficacy of the medicinal plant commonly used in the traditional medicine system in the treatment and management of illnesses by the T’boli tribe in South Cotabato

scholarly journals Synthesis, spectroscopic characterization and biological application of copper complex of 5-carbethoxy-2-thiouracil

2020 ◽  
Vol 10 (6) ◽  
pp. 145-148
Author(s):  
Brajesh Kumar ◽  
Abhishek Suman
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Fetal Lung ◽  
In Vitro Cytotoxicity ◽  
Mixed Ligand ◽  
Carcinoma Cell Line ◽  
Mixed Ligand Complexes ◽  
High Concentration

5-carbethoxy-2-thiouracil (eitotH2) reacts with CuX (X= Cl, Br, I) halides to give the formula [CuX(eitotH)2]2 dinuclear complexes, while the formula [CuX(PPh3)2(eitotH)2] mononuclear mixed ligand complexes result when reaction is carried out in the presence of two equivalent of triphenylphosphine (PPh3). The new copper (I) complexes were studied against two tumor cell lines, A549 (human pulmonary carcinoma cell line) and HeLa (human epithelial carcinoma cell line) and one regular immortalized cell line, MRC5 (human fetal lung fibroblast). In comparison to the phosphine free ones that hindered cell proliferation only at relatively high concentration, the mixed ligand complexes with triphenylphosphine were found to be extremely cytotoxic. Keywords: Copper (I), 5-carbethoxy-2-thiouracil (eitotH2), Triphenylphosphine, in vitro cytotoxicity, carcinoma cell lines

scholarly journals Nonhematopoietic tumor cell lines express stem cell factor and display c-kit receptors

Blood ◽  
1992 ◽  
Vol 80 (2) ◽  
pp. 374-381 ◽  
Author(s):  
AM Turner ◽  
KM Zsebo ◽  
F Martin ◽  
FW Jacobsen ◽  
LG Bennett ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Melanoma Cell ◽  
Melanoma Cell Line ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Tumor Cell Lines ◽  
Cell Lung Carcinoma ◽  
High Affinity

Abstract Human stem cell factor (SCF) acts in the presence of other growth factors to stimulate the growth of primitive hematopoietic progenitor cells. These effects are performed by activation of the SCF receptor, c- kit. Because of the potential use of SCF in patients undergoing chemotherapy and bone marrow transplantation, the effect of SCF on nonhematopoietic tumors requires investigation. To determine whether human tumor cell lines display c-kit receptors, we performed binding experiments with 125I-SCF on a breast carcinoma cell line (Du4475), a gastric carcinoma cell line (KATO III), a melanoma cell line (HTT144), as well as two small cell lung carcinoma cell lines (H69 and H128). The biologic effect of SCF on tumor cell lines was assessed by its ability to stimulate tritiated thymidine uptake and to enhance colony growth in methylcellulose. The breast carcinoma cell line, Du4475, as well as two small cell lung carcinoma cell lines, H69 and H128, exhibit high- affinity c-kit receptors with approximate binding affinities of 40, 100, and 90 pmol/L, respectively. The number of high-affinity receptors per cell ranged from 700 to 9,500. The gastric carcinoma cell line, as well as the melanoma cell line, showed trace binding of 125I-SCF. In the presence of SCF alone, or in combination with granulocyte- macrophage colony-stimulating factor or interleukin-3, there was less than a 17% increase in the colony growth of Du4475, H69, or H128 cell lines. Postulating that the lack of growth response could be secondary to endogenous SCF production by the tumor cell lines, we used an RNAse protection assay to determine whether the tumor cell lines contain SCF messenger RNA (mRNA). In addition, we tested tumor cell line supernatants for the presence of secreted SCF protein by enzyme immunoassay, and analyzed the tumor cell lines for membrane-bound SCF by indirect immunofluorescence. Our results show that the Du4475, H69, and H128 cell lines, as well as a melanoma cell line (HTT144), have multiple copies of SCF mRNA. Soluble SCF protein was detected in the cell supernatants in the Du4475 and H69 cell lines and SCF was found on the surface of all four cell lines. These data show that some human solid tumor cell lines display high-affinity c-kit receptors and produce SCF, which can be detected on the cell surface. These results suggest the possibility that autocrine production of SCF by c-kit receptor-bearing tumor cells may enhance cell growth in tumor cell lines.

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