scholarly journals Complete Genome Sequences of Igbo-Ora and Babanki Alphavirus Strains Isolated in the Central African Republic in the 1960s and 1970s

2019 ◽  
Vol 8 (41) ◽  
Author(s):  
Vianney Tricou ◽  
Benjamin Selekon ◽  
Ousmane Faye ◽  
Antoine Gessain ◽  
Mirdad Kazanji ◽  
...  
Keyword(s):  
Public Health ◽  
At Risk ◽  
Human Serum ◽  
Complete Genome ◽  
Central African Republic ◽  
Whole Genome ◽  
Genome Sequences ◽  
Vector Borne ◽  
1960S And 1970S ◽  
The 1960S

Vector-borne viruses are becoming increasingly important from a public health standpoint with the emergence or reemergence of viruses and extension of the areas at risk. Here, we report the whole-genome sequences of two alphaviruses, namely, one Igbo-Ora virus and one Babanki virus, that were isolated several decades ago in Africa from human serum.

scholarly journals Complete Genome Sequences of Two Chikungunya Viruses Isolated in the Central African Republic in the 1970s and 1980s

2017 ◽  
Vol 5 (9) ◽  
Author(s):  
Vianney Tricou ◽  
Marion Desdouits ◽  
Emmanuel Nakouné ◽  
Antoine Gessain ◽  
Mirdad Kazanji ◽  
...  
Keyword(s):  
Global Health ◽  
Complete Genome ◽  
Central African Republic ◽  
Complex Dynamics ◽  
Whole Genome ◽  
Genome Sequences

ABSTRACT Some arboviruses threaten human global health with potentially explosive emergence. Analysis of whole-genome sequences of decades-old isolates might contribute to the understanding of the complex dynamics which drive their circulation and emergence. Here, we report the whole-genome sequences of two Chikungunya viruses isolated in the Central African Republic in the 1970s and 1980s.

scholarly journals Complete Genome Sequences of Campylobacter hyointestinalis subsp. hyointestinalis Strain LMG 9260 and C. hyointestinalis subsp. lawsonii Strain LMG 15993

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
William G. Miller ◽  
Emma Yee ◽  
Mary H. Chapman
Keyword(s):  
Complete Genome ◽  
Whole Genome ◽  
Genome Sequences

Campylobacter hyointestinalis is isolated primarily from ruminants and swine, but is also occasionally isolated from humans. C. hyointestinalis is currently divided into two subspecies, C. hyointestinalis subsp. hyointestinalis and C. hyointestinalis subsp. lawsonii . This study describes the first closed whole-genome sequences of C. hyointestinalis subsp. hyointestinalis isolate LMG 9260 and C. hyointestinalis subsp. lawsonii isolate LMG 15993.

scholarly journals A Half-Day Genome Sequencing Protocol for Middle East Respiratory Syndrome Coronavirus

2021 ◽  
Vol 12 ◽  
Author(s):  
Kwan Woo Kim ◽  
Sungmi Choi ◽  
Su-Kyoung Shin ◽  
Imchang Lee ◽  
Keun Bon Ku ◽  
...  
Keyword(s):  
Public Health ◽  
Middle East ◽  
Genome Sequencing ◽  
Complete Genome ◽  
Middle East Respiratory Syndrome ◽  
Universal Primers ◽  
Clinical Samples ◽  
Primary Concern ◽  
Whole Genome ◽  
Serum Samples

Recent coronavirus (CoV) outbreaks, including that of Middle East respiratory syndrome (MERS), have presented a threat to public health worldwide. A primary concern in these outbreaks is the extent of mutations in the CoV, and the content of viral variation that can be determined only by whole genome sequencing (WGS). We aimed to develop a time efficient WGS protocol, using universal primers spanning the entire MERS-CoV genome. MERS and synthetic Neoromicia capensis bat CoV genomes were successfully amplified using our developed PCR primer set and sequenced with MinION. All experimental and analytical processes took 6 h to complete and were also applied to synthetic animal serum samples, wherein the MERS-CoV genome sequence was completely recovered. Results showed that the complete genome of MERS-CoV and related variants could be directly obtained from clinical samples within half a day. Consequently, this method will contribute to rapid MERS diagnosis, particularly in future CoV epidemics.

scholarly journals Whole-Genome Sequences of Three Plant Growth-Promoting Rhizobacteria Isolated from Solanum tuberosum L. Rhizosphere in Tanzania

2020 ◽  
Vol 9 (20) ◽  
Author(s):  
Becky N. Aloo ◽  
Ernest R. Mbega ◽  
Billy A. Makumba ◽  
Ines Friedrich ◽  
Robert Hertel ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Plant Growth ◽  
Complete Genome ◽  
Solanum Tuberosum L ◽  
Plant Growth Promoting Rhizobacteria ◽  
Whole Genome ◽  
Genome Sequences ◽  
Content Type ◽  
Plant Growth Promoting ◽  
Growth Promoting

We present here the complete genome sequences of plant growth-promoting Klebsiella sp. strain MPUS7, Serratia sp. strain NGAS9, and Citrobacter sp. strain LUTT5, isolated from rhizosphere soils and tubers of potato (Solanum tuberosum L.) plants growing in the northern and southern highlands of Tanzania.

scholarly journals Whole-Genome Sequences of Two Closely Related Bacteria, Actinomyces sp. Strain Chiba101 and Actinomyces denticolens DSM 20671T

2017 ◽  
Vol 5 (14) ◽  
Author(s):  
Yu Kanesaki ◽  
Taichiro Ishige ◽  
Yuriko Sekigawa ◽  
Tomoko Kobayashi ◽  
Yasushi Torii ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Whole Genome ◽  
Genome Sequences ◽  
High Quality ◽  
Content Type

ABSTRACT Actinomyces sp. strain Chiba101, isolated from an arthritic leg joint of a pig raised in Japan, is a bacterium closely related to Actinomyces denticolens. Here, we deciphered the complete genome sequence of Actinomyces sp. Chiba101 and the high-quality draft genome sequence of A. denticolens DSM 20671T.

scholarly journals Whole-genome analysis reveals the complex evolutionary dynamics of Kenyan G2P[4] human rotavirus strains

2011 ◽  
Vol 92 (9) ◽  
pp. 2201-2208 ◽  
Author(s):  
Souvik Ghosh ◽  
Noriaki Adachi ◽  
Zipporah Gatheru ◽  
James Nyangao ◽  
Dai Yamamoto ◽  
...  
Keyword(s):  
Genome Analysis ◽  
Complete Genome ◽  
Evolutionary Dynamics ◽  
Genomic Analysis ◽  
Whole Genome ◽  
Genome Sequences ◽  
Whole Genome Analysis ◽  
Human Rotavirus ◽  
Genotype Constellation ◽  
Common Causes

Although G2P[4] rotaviruses are common causes of acute childhood diarrhoea in Africa, to date there are no reports on whole genomic analysis of African G2P[4] strains. In this study, the nearly complete genome sequences of two Kenyan G2P[4] strains, AK26 and D205, detected in 1982 and 1989, respectively, were analysed. Strain D205 exhibited a DS-1-like genotype constellation, whilst strain AK26 appeared to be an intergenogroup reassortant with a Wa-like NSP2 genotype on the DS-1-like genotype constellation. The VP2-4, VP6-7, NSP1, NSP3 and NSP5 genes of strain AK26 and the VP2, VP4, VP7 and NSP1–5 genes of strain D205 were closely related to those of the prototype or other human G2P[4] strains. In contrast, their remaining genes were distantly related, and, except for NSP2 of AK26, appeared to originate from or share a common origin with rotavirus genes of artiodactyl (ruminant and camelid) origin. These observations highlight the complex evolutionary dynamics of African G2P[4] rotaviruses.

scholarly journals The first complete genome sequences of the acI lineage, the most abundant freshwater Actinobacteria, obtained by whole-genome-amplification of dilution-to-extinction cultures

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Ilnam Kang ◽  
Suhyun Kim ◽  
Md. Rashedul Islam ◽  
Jang-Cheon Cho
Keyword(s):  
Metabolic Pathways ◽  
Complete Genome ◽  
Whole Genome Amplification ◽  
Whole Genome ◽  
Bacterial Group ◽  
Freshwater Lakes ◽  
Genome Sequences ◽  
Genome Amplification ◽  
Axenic Cultures ◽  
Niche Diversification

Abstract The acI lineage of the phylum Actinobacteria is the most abundant bacterial group in most freshwater lakes. However, due to difficulties in laboratory cultivation, only two mixed cultures and some incomplete single-amplified or metagenome-derived genomes have been reported for the lineage. Here, we report the initial cultivation and complete genome sequences of four novel strains of the acI lineage from the tribes acI-A1, -A4, -A7, and -C1. The acI strains, initially isolated by dilution-to-extinction culturing, eventually failed to be maintained as axenic cultures. However, the first complete genomes of the acI lineage were successfully obtained from these initial cultures through whole genome amplification applied to more than hundreds of cultured acI cells. The genome sequences exhibited features of genome streamlining and showed that the strains are aerobic chemoheterotrophs sharing central metabolic pathways, with some differences among tribes that may underlie niche diversification within the acI lineage. Actinorhodopsin was found in all strains, but retinal biosynthesis was complete in only A1 and A4 tribes.

scholarly journals Complete Genome Sequences of Two Interactive Moderate Thermophiles,Paenibacillus napthalenovorans32O-Y andPaenibacillussp. 32O-W

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Robert R. Butler ◽  
Jia Wang ◽  
Benjamin C. Stark ◽  
Jean-François Pombert
Keyword(s):  
High Temperature ◽  
Complete Genome ◽  
Industrial Applications ◽  
Whole Genome ◽  
Genome Sequences ◽  
High Demand ◽  
Moderate Thermophiles

Microorganisms with the capability to desulfurize petroleum are in high demand with escalating restrictions currently placed on fuel purity. Thermophilic desulfurizers are particularly valuable in high-temperature industrial applications. We report the whole-genome sequences ofPaenibacillus napthalenovorans32O-Y andPaenibacillussp. 32O-W, which can and cannot, respectively, metabolize dibenzothiophene.

scholarly journals Complete Genome Sequences of Three Fish-Associated Streptococcus agalactiae Isolates

2018 ◽  
Vol 6 (6) ◽  
pp. e00025-18 ◽  
Author(s):  
Anita Jaglarz ◽  
Artur Gurgul ◽  
William J. Leigh ◽  
Janina Z. Costa ◽  
Kim D. Thompson
Keyword(s):  
United States ◽  
Costa Rica ◽  
Streptococcus Agalactiae ◽  
Oreochromis Niloticus ◽  
Complete Genome ◽  
The United States ◽  
Whole Genome ◽  
Genome Sequences ◽  
Group B

ABSTRACTThe whole-genome sequences are described here for three group B Streptococcus (GBS) (S. agalactiae) serotype Ib isolates obtained from tilapia (Oreochromis niloticus) farmed at sites in Honduras, Costa Rica, and the United States. The bacteria were isolated from the brains of fish displaying signs of streptococcosis.

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