scholarly journals Citrobacter Species Increase Energy Harvest by Modulating Intestinal Microbiota in Fish: Nondominant Species Play Important Functions

mSystems ◽  
2020 ◽  
Vol 5 (3) ◽  
Author(s):  
Mei-Ling Zhang ◽  
Miao Li ◽  
Yi Sheng ◽  
Fang Tan ◽  
Liqiao Chen ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Intestinal Microbiota ◽  
Lipid Accumulation ◽  
High Fat Diet ◽  
High Throughput Sequencing ◽  
Intestinal Bacteria ◽  
Absorption Efficiency ◽  
Energy Harvest ◽  
High Fat ◽  
Microbial Composition

ABSTRACT An efficient energy harvesting mechanism is likely critical for animals in their natural environment. Intestinal microbiota enriched by a high-fat diet aid in lipid accumulation, a strategy likely evolved for energy harvest in mammals. However, whether this strategy is conserved among vertebrate organisms remains unclear. A bacterial strain (S1), enriched on soybean oil rich medium, was isolated from the gut of Nile tilapia and demonstrated to be a member of the Citrobacter genus. Although a high-fat diet increased the number of Citrobacter spp., these bacteria were not abundant in the intestine by high-throughput sequencing. Addition of bacterium S1 to a high-fat diet modulated intestinal microbial composition and increased high-fat diet-induced lipid accumulation in mesenteric adipose tissue, accompanied by (i) increased triglyceride absorption efficiency and triglyceride reesterification and (ii) increased intestinal permeability. Collectively, our results provide evidence that specific intestinal bacteria aid the host in harvesting more energy from a high-fat diet in fish. Furthermore, the results from the present study also suggest that nondominant bacteria in the gut may play an important role in regulating host metabolism. IMPORTANCE This study shows that the ability of gut microbiota members to enhance host energy harvest from a high-fat diet is a conserved feature of host-microbe interactions in fish, as in mammals. It also underscores that gut microbiota members are able to significantly impact host biology even when at low abundance.

Uridine attenuates obesity, ameliorates hepatic lipid accumulation and modifies the gut microbiota composition in mice fed with a high-fat diet

2021 ◽  
Author(s):  
Yilin Liu ◽  
Chunyan Xie ◽  
Zhenya Zhai ◽  
Ze-yuan Deng ◽  
Hugo R. De Jonge ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Lipid Accumulation ◽  
High Fat Diet ◽  
Microbiota Composition ◽  
High Fat ◽  
Fat Accumulation ◽  
Hepatic Lipid ◽  
Hepatic Lipid Accumulation ◽  
Gut Microbiota Composition

This study aimed to investigate the effect of uridine on obesity, fat accumulation in liver, and gut microbiota composition in high-fat diet-fed mice.

scholarly journals Effect of A Polyphenol-Rich Canarium album Extract on the Composition of the Gut Microbiota of Mice Fed a High-Fat Diet

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2188 ◽  
Author(s):  
Ning-Ning Zhang ◽  
Wen-Hui Guo ◽  
Han Hu ◽  
A-Rong Zhou ◽  
Qing-Pei Liu ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
High Fat Diet ◽  
High Throughput Sequencing ◽  
Chow Diet ◽  
Microbiota Composition ◽  
High Fat ◽  
Canarium Album ◽  
Normal Chow ◽  
Gut Microbiota Composition ◽  
Medium Dose

This study investigated the influence of Canarium album extract (CAext) on intestinal microbiota composition of mice fed a high-fat diet (HFD). Kun Ming (KM) mice were fed either a normal chow diet or a HFD for six weeks. At the seventh week, HFD-fed mice were gavaged daily with saline, or a different dose of CAext for four weeks, respectively. Then, the composition of the gut microbiota was analyzed by high-throughput sequencing technology. Analysis of fecal microbial populations, grouped by phyla, showed significant increases of Firmicutes and Verrucomicrobia, but a decrease of Bacteroidetes in all CAext-fed mice. Particularly, CAext gavage in a low dose or a medium dose caused a significant increase in the proportion of Akkermansia. These findings suggested that CAext can alter the gut microbiota composition of HFD-fed mice, and had a potential prebiotic effects on Akkermansia.

scholarly journals Glucosamine Ameliorates Symptoms of High-Fat Diet-Fed Mice by Reversing Imbalanced Gut Microbiota

2021 ◽  
Vol 12 ◽  
Author(s):  
Xubing Yuan ◽  
Junping Zheng ◽  
Lishi Ren ◽  
Siming Jiao ◽  
Cui Feng ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
High Fat Diet ◽  
Inflammatory Responses ◽  
Fasting Blood Glucose ◽  
Intestinal Barrier ◽  
Intestinal Bacteria ◽  
High Fat ◽  
Lipid Metabolism Disorder ◽  
Metabolism Disorder ◽  
Rdna Sequencing

Glucosamine (GlcN) is used as a supplement for arthritis and joint pain and has been proved to have effects on inflammation, cancer, and cardiovascular diseases. However, there are limited studies on the regulatory mechanism of GlcN against glucose and lipid metabolism disorder. In this study, we treated high-fat diet (HFD)-induced diabetic mice with GlcN (1 mg/ml, in drinking water) for five months. The results show that GlcN significantly reduced the fasting blood glucose of HFD-fed mice and improved glucose tolerance. The feces of intestinal contents in mice were analyzed using 16s rDNA sequencing. It was indicated that GlcN reversed the imbalanced gut microbiota in HFD-fed mice. Based on the PICRUSt assay, the signaling pathways of glucolipid metabolism and biosynthesis were changed in mice with HFD feeding. By quantitative real-time PCR (qPCR) and hematoxylin and eosin (H&E) staining, it was demonstrated that GlcN not only inhibited the inflammatory responses of colon and white adipose tissues, but also improved the intestinal barrier damage of HFD-fed mice. Finally, the correlation analysis suggests the most significantly changed intestinal bacteria were positively or negatively related to the occurrence of inflammation in the colon and fat tissues of HFD-fed mice. In summary, our studies provide a theoretical basis for the potential application of GlcN to glucolipid metabolism disorder through the regulation of gut microbiota.

scholarly journals Inulin from Jerusalem artichoke tubers alleviates hyperglycaemia in high-fat-diet-induced diabetes mice through the intestinal microflora improvement

2019 ◽  
Vol 123 (3) ◽  
pp. 308-318 ◽  
Author(s):  
Tianyun Shao ◽  
Qiuhong Yu ◽  
Tingshuo Zhu ◽  
Anhong Liu ◽  
Xiumei Gao ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Intestinal Microbiota ◽  
High Fat Diet ◽  
High Throughput Sequencing ◽  
Fasting Blood Glucose ◽  
Jerusalem Artichoke ◽  
Differentially Expressed ◽  
High Fat ◽  
Blood Concentrations ◽  
Linear Discriminant

AbstractThe rate of hyperglycaemia in people around the world is increasing at an alarming rate at present, and innovative methods of alleviating hyperglycaemia are needed. The effects of Jerusalem artichoke inulin on hyperglycaemia, liver-related genes and the intestinal microbiota in mice fed a high-fat diet (HFD) and treated with streptozotocin (STZ) to induce hyperglycaemia were investigated. Inulin-treated hyperglycaemic mice had decreased average daily food consumption, body weight, average daily water consumption and relative liver weight and blood concentrations of TAG, total cholesterol, HDL-cholesterol and fasting blood glucose. Liver-related gene expressions in hyperglycaemic (HFD-fed and STZ-treated) compared with control mice showed eighty-four differentially expressed genes (forty-nine up-regulated and thirty-five down-regulated). In contrast, hyperglycaemic mice treated with inulin had twenty-two differentially expressed genes compared with control ones. Using Illumina high-throughput sequencing technology, the rarefaction and the rank abundance curves as well as the α diversity indices showed the treatment-induced differences in bacterial diversity in intestine. The linear discriminant analysis of effect size showed that the inulin treatment improved intestinal microbiota; in particular, it significantly increased the number of Bacteroides in the intestine of mice. In conclusion, inulin is potentially an effective functional food for the prevention and/or treatment of hyperglycaemia.

scholarly journals Phosphatidylserine from Portunus trituberculatus Eggs Alleviates Insulin Resistance and Alters the Gut Microbiota in High-Fat-Diet-Fed Mice

Marine Drugs ◽  
2020 ◽  
Vol 18 (9) ◽  
pp. 483
Author(s):  
Shiwei Hu ◽  
Mengyu Du ◽  
Laijin Su ◽  
Huicheng Yang
Keyword(s):  
Insulin Resistance ◽  
Gut Microbiota ◽  
High Fat Diet ◽  
Glucose Transporter ◽  
Animal Experiments ◽  
Intestinal Bacteria ◽  
Food Supplement ◽  
Portunus Trituberculatus ◽  
Gut Health ◽  
High Fat

Portunus trituberculatus eggs contain phospholipids, whose components and bioactivity are unclear. Here, we investigated the fatty acid composition of phosphatidylserine from P. trituberculatus eggs (Pt-PS). Moreover, its effects on insulin resistance and gut microbiota were also evaluated in high-fat-diet-fed mice. Our results showed that Pt-PS accounted for 26.51% of phospholipids and contained abundant polyunsaturated fatty acids (more than 50% of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)). Animal experiments indicated that Pt-PS significantly decreased body weight and adipose weight gain, improved hyperglycemia and hyperinsulinemia, mitigated insulin resistance, and regulated circulatory cytokines. Pt-PS activated insulin receptor substrate 1 (IRS1) and increased the levels of IRS1-associated phosphatidylinositol 3-hydroxy kinase (PI3K), phosphorylated protein kinase B (Akt) protein, and plasma membrane glucose transporter 4 protein. Furthermore, Pt-PS modified the gut microbiota, inducing, especially, a dramatic decrease in the ratio of Firmicutes to Bacteroidetes at the phylum level, as well as a remarkable improvement in their subordinate categories. Pt-PS also reduced fecal lipopolysaccharide concentration and enhanced fecal acetate, propionate, and butyrate concentrations. Additionally, the effects of Pt-PS on alleviation of insulin resistance and regulation of intestinal bacteria were better than those of phosphatidylserine from soybean. These results suggest that Pt-PS mitigates insulin resistance by altering the gut microbiota. Therefore, Pt-PS may be developed as an effective food supplement for the inhibition of insulin resistance and the regulation of human gut health.

Fermented Soy Paste Alleviates Lipid Accumulation in the Liver by Regulating the AMPK Pathway and Modulating Gut Microbiota in High-Fat-Diet-Fed Rats

2020 ◽  
Vol 68 (35) ◽  
pp. 9345-9357 ◽  
Author(s):  
Yen-Chen Tung ◽  
Zhi-Rong Liang ◽  
San-Fang Chou ◽  
Chi-Tang Ho ◽  
Yu-Lun Kuo ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Lipid Accumulation ◽  
High Fat Diet ◽  
High Fat ◽  
Ampk Pathway

scholarly journals Dietary Polysaccharide from Enteromorpha clathrata Attenuates Obesity and Increases the Intestinal Abundance of Butyrate-producing Bacterium, Eubacterium xylanophilum, in Mice Fed a High-Fat Diet

Polymers ◽  
2021 ◽  
Vol 13 (19) ◽  
pp. 3286
Author(s):  
Jiali Wei ◽  
Yiran Zhao ◽  
Chen Zhou ◽  
Qing Zhao ◽  
Hongqian Zhong ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
High Fat Diet ◽  
High Throughput Sequencing ◽  
Serum Levels ◽  
Bioinformatic Analysis ◽  
The Body ◽  
Therapeutic Effects ◽  
High Fat ◽  
Intestinal Dysbiosis ◽  
Treatment Of Obesity

Previous studies have suggested that polysaccharide from Enteromorpha clathrata (ECP) could be used as a potential prebiotic to treat dysbiosis-associated diseases. However, whether it has any therapeutic effects on obesity has not been investigated. In the present study, we explored the anti-obesity effect of ECP and illustrated that it can significantly reduce the body weight and decrease the serum levels of triacylglycerol and cholesterol in high-fat diet (HFD)-fed mice. As revealed by 16S rRNA high-throughput sequencing and bioinformatic analysis, HFD remarkably changed the composition of the gut microbiota and promoted the growth of opportunistic pathogens such as Mucispirillum, Desulfobacterota and Alphaproteobacteria in obese mice. Interestingly, ECP improved intestinal dysbiosis caused by HFD and reshaped the structure of the gut microbiota in diseased mice by increasing the abundance of butyrate-producing bacterium, Eubacterium xylanophilum, in the gut. Altogether, we demonstrate for the first time an anti-obesity effect of ECP and shed new light into its therapeutic mechanisms from the perspective of gut microbiota. Our study will pave the way for the development of ECP as new prebiotic for the treatment of obesity and its associated disorders.

scholarly journals Comparative analysis of gut bacterial communities in housefly larvae fed different diets using a high-throughput sequencing approach

2019 ◽  
Vol 366 (11) ◽  
Author(s):  
Zhijing Xue ◽  
Junli Zhang ◽  
Ruiling Zhang ◽  
Zhendong Huang ◽  
Qing Wan ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
High Throughput ◽  
Bacterial Communities ◽  
High Fat Diet ◽  
High Throughput Sequencing ◽  
Animal Feed ◽  
Intestinal Bacteria ◽  
High Protein ◽  
Fish Feed ◽  
High Fat

ABSTRACT Housefly larvae are a synanthropic host for various bacteria, including pathogens and commensals and an important protein source for monogastric animal feed. Many factors, such as diets, life stages, host habitats can influence microbial community structure. In this study, the diversity of bacterial communities in the gut of housefly larvae fed on different artificial diets was comprehensively characterized using high-throughput sequencing with the aim shedding light on an optimal larval diet. The results showed that the dominant bacteria belonging to Proteobacteria, Firmicutes and Bacteroidetes phyla were related to polysaccharide degradation. The comparative analysis indicated that the dominant intestinal bacteria of larvae fed on high-protein were similar to those on high-fat diet. The same was the case in larvae fed high-starch diet and wheat bran alone. In addition, the diversity of intestinal bacteria at genus level in larvae fed high-protein and high-fat diet was higher than in larvae fed the other two diets. Further analysis indicated that the increase of potential commensals and decrease of pathogens in larvae fed on high-fat diet contributed to the increase of housefly larvae immunity. It established a foundation for further research on improvement of nutrition of housefly larvae used for poultry and fish feed.

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