Identification and Characterization of Glucosyltransferase That Forms 1-Galloyl-β-d-Glucogallin in Canarium album L., a Functional Fruit Rich in Hydrolysable Tannins

Hydrolysable tannins (HTs) are useful secondary metabolites that are responsible for pharmacological activities and astringent taste, flavor, and quality in fruits. They are also the main polyphenols in Canarium album L. (Chinese olive) fruit, an interesting and functional fruit that has been cultivated for over 2000 years. The HT content of C. album fruit was 2.3–13 times higher than that of berries with a higher content of HT. 1-galloyl-β-d-glucose (βG) is the first intermediate and the key metabolite in the HT biosynthesis pathway. It is catalyzed by UDP-glucosyltransferases (UGTs), which are responsible for the glycosylation of gallic acid (GA) to form βG. Here, we first reported 140 UGTs in C. album. Phylogenetic analysis clustered them into 14 phylogenetic groups (A, B, D–M, P, and Q), which are different from the 14 typical major groups (A~N) of Arabidopsis thaliana. Expression pattern and correlation analysis showed that UGT84A77 (Isoform0117852) was highly expressed and had a positive correlation with GA and βG content. Prokaryotic expression showed that UGT84A77 could catalyze GA to form βG. These results provide a theoretical basis on UGTs in C. album, which will be helpful for further functional research and availability on HTs and polyphenols.

Same useful fixative from resin in Vietnam

Today, fixatives from natural raw materials are gradually replacing fixatives synthesized from chemicals. The arm of this research is the study of same useful resin in Vietnam for fixative substance in blending fragrance. We obtained Benzoin resin from Styrax tonkenensis Pierre plant in Ha Giang province, Canarium resin from Canarium Album L. in Dak Nong district, Dak Lak province, and Agarwood pulp of Aquilaria crassna plant in Binh Thuan province Vietnam. The material used in the experiment for the natural fragrance was taken from the project of Vietnam essential oils and related natural products. The method of this process is resin extraction by volatile solvents. The resin is dissolved in alcohol 96% and the distilled alcohol is removed to obtain absolute. The method of assessment of product quality in this study is using the olfactory to assess the odour of samples over time. Benzoin resin, Canarium resin, and Agarwood resin of Vietnam are useful fixatives in blending fragrance. The fixative ability of Benzoin resin absolute is not equal to the ability of Agarwood resin absolute but better than the ability of Canarium resin absolute. Through research and experiment, we can see Benzoin resin, Canarium resin and Agarwood resin are precious. They can be used as a good fixative in aromatherapy. This is a natural resin, a kind of resource available in Vietnam. Therefore, it is recommended for further research, exploitation, and effective use of this resource.

Olive Fruit Extracts Supplement Improve Antioxidant Capacity via Altering Colonic Microbiota Composition in Mice

Oxidative stress, one of the most common biological dysfunctions, is usually associated with pathological conditions and multiple diseases in humans and animals. Chinese olive fruit (Canarium album L.) extracts (OE) are natural plant extracts rich in polyphenols (such as hydroxytyrosol, HT) and with antioxidant, anti-hyperlipidemia, and anti-inflammatory potentials. This study was conducted to investigate the antioxidant capacity of OE supplementation and its related molecular mechanism in mice. Mice (25.46 ± 1.65 g) were treated with 100 mg/kg body weight (BW) OE or saline solution for 4 weeks, and then the antioxidant and anti-inflammatory capacities of mice were examined. The results showed that OE supplement significantly increased the serum antioxidative enzyme activities of total antioxidant activity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase and decreased the serum malondialdehyde (MDA) level, indicating that OE treatment enhanced the antioxidant capacity in mice. qPCR results showed that the transcriptional expression of antioxidant SOD1, CAT, Gpx1, and Gpx2 were significantly down-regulated in the small intestine (jejunum and ileum) after OE administration. Meanwhile, OE treatment significantly decreased the T-AOC and increased the MDA level in the small intestine. Furthermore, OE administration dramatically reduced the mRNA expression of pro-inflammatory cytokines (TNF-α and IL-1β), which confirmed its antioxidant and anti-inflammatory capacities with OE administration. Using amplicon sequencing technology, 16S rRNA sequencing results showed that OE supplement significantly increased the colonic Firmicutes/Bacteroidetes ratio, which also had a negative correlation with the serum MDA level and positively correlated with serum GSH-Px activity through Pearson correlation analysis. Besides that, Alloprevotella was negatively correlated with serum T-AOC. Colidextribacter was positively correlated with serum MDA and negatively correlated with serum T-AOC, SOD, and GSH-Px levels. In summary, this study showed that treatment with 100 mg/kg BW polyphenol-rich OE could alter colonic microbiota community, which was strongly associated with improved antioxidant capacity in mice.

ĐÁNH GIÁ TÌNH HÌNH SINH TRƯỞNG CỦA CÁC LOÀI CÂY BẢN ĐỊA THEO CÁC DẠNG LẬP ĐỊA KHÁC NHAU: NGHIÊN CỨU TRƯỜNG HỢP VỚI 3 LOÀI CÂY LIM XANH, TRÁM TRẮNG VÀ HUỶNH TẠI TỈNH QUẢNG BÌNH

Bài báo trình bày kết quả đánh giá sự phù hợp, sinh trưởng và phát triển của các loài cây trồng bản địa trên các dạng lập địa khác nhau tại tỉnh Quảng Bình. Kết quả phân tích tại các ô theo dõi đo đếm và bố trí thí nghiệm trên các loài Lim xanh (Erythrophleum fordi Oliv), Trám trắng (Canarium album Raeusch), Huỷnh (Tarrietia javanica Blume) từ năm 1 đến năm 6 cho thấy tỷ lệ sống của các loài đạt khá cao, trên 82%; Lượng tăng trưởng bình quân tới tuổi 6 về đường kính của các loài cây dao động từ 1,46-1,95cm/năm; Biện pháp xử lý thự bì có ảnh hưởng rõ rệt tới sinh trưởng chiều cao và đường kính ngang ngực của các loài. Công thức xử lý thực bì phù hợp là xử lý thực bì theo băng. Các dạng lập địa khác nhau ảnh hưởng đến tỷ lệ sống và các chỉ tiêu sinh trưởng của các loài. Cây Lim xanh và cây Trám trắng thích hợp hơn với dạng lập địa B.

Using the Natural Fixative from Canarium Resin of Vietnam

Aim and Objectives: Nowadays, the fixative substance from natural materials is gradually replacing the fixative substance that has been synthesized from chemicals. The purpose of the study in this research is that we created a natural fragrance from Vietnamese raw material with a fixative from Canarium Album (Lour) as a natural fixative to replace artificial fixative. Canarium plant is scientifically known as Canarium Album (Lour) Raensch of the Burseraceae family. Canarium Album L. is distributed in most of Northern provinces, central Highlands, and Southeast of Vietnam. Materials and Methods: The method of this process is resin extraction by volatile solvents. We use a knife to cut into the trunk and let the resin run out, then harvest this resin to make fixative. The resin is dissolved in alcohol 96% and the distilled alcohol is removed to obtain absolute Canarium. Evaluation method of the ability of absolute Canarium is performed by comparing it with other fixatives in fragrances. Raw canarium resins is taken directly from canarium plants then refined absolute canarium as the natural fixative. We use this fixative substance in combinations of floral, woody smells and comparison with traditional artificial fixative as Musk ketone, Musk xylene, Musk ambrette. Results: The comparison results show that the ability to keep the fragrance of scent is lower than musk ambrette, musk ketone but it is better than musk xylene. Thus, canarium resin can be used as a natural fixative to replace artificial fixative in fragrance. Conclusions: Through research and experiment, we can see Canarium resin is precious. It can be used as a good fixative in aromatherapy. This is a natural resin, a kind of resource available in Vietnam. Therefore, it is recommended for further research, exploitation, and effective use of this resource.

Antioxidant phenolic compounds from Chinese white olive (Canarium album L.)

To find antioxidant compounds from Chinese white olive (CWO) based on bioassay-guided method, the ethanol extract and corresponding 4 fractions were evaluated for their antioxidant activities by DPPH and ABTS assay. The results revealed that the AcOEt fraction had the most potent antioxidant activity. Further, six compounds isolated from the AcOEt fraction, and their structure were identified as (-)-epicatechin gallate (i), ellagic acid (ii), gallic acid (iii), (-)-epigallocatechin (iv), (-)-epigallocatechin gallate (v) and kaempferol (VI) by MS and NMR spectrum. Compounds I, IV and V were isolated and identified from CWO for the first time. All the compounds (I-VI) exhibited potent antioxidant activity. Among them, compound III showed best antioxidant activity in both DPPH and ABTS+ assay. The phenolic compounds from CWO with potent antioxidant activities were responsible for the antioxidant activity of CWO extract.