Studying the Mechanism of Dioxidine Genotoxicity Using Lux Biosensors of Esсherichia coli

2021 ◽  
Vol 48 (12) ◽  
pp. 2174-2180
Author(s):  
D. A. Sviridova ◽  
E. A. Machigov ◽  
E. V. Igonina ◽  
B. S. Zhoshibekova ◽  
S. K. Abilev
Keyword(s):  
Lux Biosensors

Toxic Effect of 2-ethyl (bicyclo[2.2.1] heptane) on Bacterial Cells

2019 ◽  
Vol 35 (6) ◽  
pp. 67-72 ◽  
Author(s):  
I.V. Manukhov ◽  
L.S. Yaguzhinsky ◽  
M.V. Bermeshev ◽  
M.A. Zisman ◽  
V.G. Pevgov ◽  
...  
Keyword(s):  
Toxic Effect ◽  
Atmospheric Oxygen ◽  
Inducible Promoter ◽  
Oxygen Species ◽  
Bacterial Cells ◽  
Unique Identifier ◽  
E Coli ◽  
Lux Biosensors ◽  
High Level ◽  
Ministry Of Higher Education

Toxic effect of 2-ethylnorbornane (2-ethyl(bicyclo[2.2.1]heptane) (EBH)) on bacteria has been studied using the E. coli pRecA-lux and E. coli pKatG- lux cells as lux-biosensors. It was shown that the addition of EBH to the incubation medium leads to death and growth retardation, high level oxidative stress and DNA damage in E. coli cells. It is assumed that the oxidation of EBH with atmospheric oxygen causes the formation of reactive oxygen species in the medium, which makes a major contribution to the toxicity of this substance. biosensor, luciferase, bioluminescence, inducible promoter, PrecA, PkatG The authors are grateful to Stanislav Filippovich Chalkin for the development of interdisciplinary ties in the scientific community. The work was financially supported by the Ministry of Higher Education and Science of Russia (Project Unique Identifier RFMEFI60417X0181, Agreement No. 14.604.21.0181 of 26.09.2017).

scholarly journals Influence of the luxR Regulatory Gene Dosage and Expression Level on the Sensitivity of the Whole-Cell Biosensor to Acyl-Homoserine Lactone

Biosensors ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 166
Author(s):  
Sergey Bazhenov ◽  
Uliana Novoyatlova ◽  
Ekaterina Scheglova ◽  
Vadim Fomin ◽  
Svetlana Khrulnova ◽  
...  
Keyword(s):  
Gene Dosage ◽  
Regulatory Gene ◽  
Homoserine Lactone ◽  
Threshold Concentration ◽  
Expression Level ◽  
Whole Cell ◽  
Lux Biosensors ◽  
Order Of Magnitude ◽  
Whole Cell Biosensor ◽  
Cell Biosensor

Aliivibrio fischeri LuxR and Aliivibrio logei LuxR1 and LuxR2 regulatory proteins are quorum sensing transcriptional (QS) activators, inducing promoters of luxICDABEG genes in the presence of an autoinducer (3-oxo-hexanoyl-l-homoserine lactone). In the Aliivibrio cells, luxR genes are regulated by HNS, CRP, LitR, etc. Here we investigated the role of the luxR expression level in LuxI/R QS system functionality and improved the whole-cell biosensor for autoinducer detection. Escherichia coli-based bacterial lux-biosensors were used, in which Photorhabdus luminescensluxCDABE genes were controlled by LuxR-dependent promoters and luxR, luxR1, or luxR2 regulatory genes. We varied either the dosage of the regulatory gene in the cells using additional plasmids, or the level of the regulatory gene expression using the lactose operon promoter. It was shown that an increase in expression level, as well as dosage of the regulatory gene in biosensor cells, leads to an increase in sensitivity (the threshold concentration of AI is reduced by one order of magnitude) and to a two to threefold reduction in response time. The best parameters were obtained for a biosensor with an increased dosage of luxRA. fischeri (sensitivity to 3-oxo-hexanoyl-l-homoserine lactone reached 30–100 pM).

Lux-biosensors for detection of SOS-response, heat shock, and oxidative stress

2010 ◽  
Vol 46 (8) ◽  
pp. 781-788 ◽  
Author(s):  
V. Yu. Kotova ◽  
I. V. Manukhov ◽  
G. B. Zavilgelskii
Keyword(s):  
Oxidative Stress ◽  
Heat Shock ◽  
Sos Response ◽  
Lux Biosensors ◽  
And Oxidative Stress

Specific Lux Biosensors of Escherichia coli Containing pRecA::lux, pColD::lux, and pDinI::lux Plasmids for Detection of Genotoxic Agents

2020 ◽  
Vol 56 (6) ◽  
pp. 666-673
Author(s):  
S. K. Abilev ◽  
V. Y. Kotova ◽  
S. V. Smirnova ◽  
T. N. Shapiro ◽  
G. B. Zavilgelsky
Keyword(s):  
Escherichia Coli ◽  
Genotoxic Agents ◽  
Lux Biosensors

Inducible Specific Lux-Biosensors for the Detection of Antibiotics: Construction and Main Parameters

2014 ◽  
Vol 50 (1) ◽  
pp. 112-117 ◽  
Author(s):  
V. Yu. Kotova ◽  
K. V. Ryzhenkova ◽  
I. V. Manukhov ◽  
G. B. Zavilgelsky
Keyword(s):  
Lux Biosensors

Inducible specific lux-biosensors for the detection of antibiotics: Construction and main parameters

2013 ◽  
Vol 50 (1) ◽  
pp. 98-103 ◽  
Author(s):  
V. Yu. Kotova ◽  
K. V. Ryzhenkova ◽  
I. V. Manukhov ◽  
G. B. Zavilgelsky
Keyword(s):  
Lux Biosensors

scholarly journals Constructing of Bacillus subtilis-Based Lux-Biosensors with the Use of Stress-Inducible Promoters

2021 ◽  
Vol 22 (17) ◽  
pp. 9571
Author(s):  
Andrew G. Kessenikh ◽  
Uliana S. Novoyatlova ◽  
Sergey V. Bazhenov ◽  
Eugeniya A. Stepanova ◽  
Svetlana A. Khrulnova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Bacillus Subtilis ◽  
Specific Response ◽  
Mechanisms Of Toxicity ◽  
Inducible Promoters ◽  
E Coli ◽  
Lux Biosensors ◽  
Increased Sensitivity ◽  
Lux Biosensor ◽  
Stress Promoter

Here, we present a new lux-biosensor based on Bacillus subtilis for detecting of DNA-tropic and oxidative stress-causing agents. Hybrid plasmids pNK-DinC, pNK-AlkA, and pNK-MrgA have been constructed, in which the Photorhabdus luminescens reporter genes luxABCDE are transcribed from the stress-inducible promoters of B. subtilis: the SOS promoter PdinC, the methylation-specific response promoter PalkA, and the oxidative stress promoter PmrgA. The luminescence of B. subtilis-based biosensors specifically increases in response to the appearance in the environment of such common toxicants as mitomycin C, methyl methanesulfonate, and H2O2. Comparison with Escherichia coli-based lux-biosensors, where the promoters PdinI, PalkA, and Pdps were used, showed generally similar characteristics. However, for B. subtilis PdinC, a higher response amplitude was observed, and for B. subtilis PalkA, on the contrary, both the amplitude and the range of detectable toxicant concentrations were decreased. B. subtilis PdinC and B. subtilis PmrgA showed increased sensitivity to the genotoxic effects of the 2,2′-bis(bicyclo [2.2.1] heptane) compound, which is a promising propellant, compared to E. coli-based lux-biosensors. The obtained biosensors are applicable for detection of toxicants introduced into soil. Such bacillary biosensors can be used to study the differences in the mechanisms of toxicity against Gram-positive and Gram-negative bacteria.

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