Formation of the paratomic fission zone in freshwater oligochaetes

2006 ◽  
Vol 37 (6) ◽  
pp. 354-365 ◽  
Author(s):  
A. V. Kharin ◽  
I. V. Zagainova ◽  
R. P. Kostyuchenko
Keyword(s):  
Freshwater Oligochaetes

scholarly journals Simultaneous preservation of the DNA quality, the community composition and the density of freshwater oligochaetes for the development of genetically based biological indices

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e6050 ◽  
Author(s):  
Régis Vivien ◽  
Inge Werner ◽  
Benoit J.D. Ferrari
Keyword(s):  
Community Composition ◽  
Sediment Quality ◽  
Low Ph ◽  
Coi Gene ◽  
Absolute Ethanol ◽  
Sediment Samples ◽  
Biological Indices ◽  
Dna Quality ◽  
Freshwater Oligochaetes ◽  
Formalin Fixed

IntroductionOligochaetes are recognized as valuable bioindicators of sediment quality in streams and lakes. The development of an oligochaete index based on the identification of specimens using DNA barcodes requires a method for simultaneously preserving the DNA quality and information on the specimen density and oligochaete community composition. Absolute ethanol optimally preserves DNA but fixation of freshwater oligochaetes with this medium can cause disintegration and fragmentation of specimens. Here, we investigated the possibility to preserve oligochaete specimens in low-pH formalin and in neutral buffered formalin for up to four weeks before genetic analyses and tested if the addition of absolute ethanol to formalin-fixed oligochaetes resulted in a loss of specimens and/or species.MethodsWe performed guanidine extraction and polymerase chain reaction (PCR) amplification/sequencing of a fragment of the cytochrome c oxidase I (COI) gene on tissue fragments preserved in low-pH formalin for up to 3 weeks and in neutral buffered formalin for up to 4 weeks. In addition, we compared the density and taxonomic composition of formalin-fixed oligochaetes of several sieved sediment samples before and after the addition of absolute ethanol.ResultsThe COI fragment of all oligochaete specimens preserved in neutral buffered formalin for up to 28 days was successfully amplified by PCR and obtained sequences were complete and of high quality. The amplification success rate for low-pH formalin fixed specimens declined after 7 days of storage. The addition of absolute ethanol to formalin-fixed oligochaete communities did not alter density or diversity estimates.DiscussionOur results indicate that sediment samples can be stored in neutral buffered formalin for up to 4 weeks and the sieved material can then be transferred to absolute ethanol, without affecting DNA quality, density and community composition of oligochaetes. Based on these results, a protocol for preserving freshwater oligochaetes, describing all the steps from collection of sediments to preservation of the biological material in absolute ethanol, is proposed. This method of fixation/preservation is of relevance for establishing DNA barcode reference databases, inventories of genetic diversity and developing genetically based biological indices.

scholarly journals Freshwater oligochaetes (Annelida) from Western Ghats andthe west coast of Karnataka (India)

2015 ◽  
Vol 39 ◽  
pp. 523-526
Author(s):  
Prashantha Ramachandra HEGDE ◽  
Kanale Sreenivasappa SREEPADA
Keyword(s):  
West Coast ◽  
Western Ghats ◽  
Freshwater Oligochaetes

Three new species of Tubificinae, Oligochaeta, from two plateau lakes in Southwest China

Zootaxa ◽  
2009 ◽  
Vol 2143 (1) ◽  
pp. 45-54 ◽  
Author(s):  
YONGDE CUI ◽  
HONGZHU WANG
Keyword(s):  
New Species ◽  
Endemic Species ◽  
Yunnan Province ◽  
Southwest China ◽  
Middle Portion ◽  
Xingyun Lake ◽  
Three New Species ◽  
Freshwater Oligochaetes

Three new species of Tubificinae (Naididae, Oligochaeta), Varichaetadrilus vestibulatus n. sp., Aulodrilus apeniatus n. sp., and Ilyodrilus mesoprostatus n. sp., are reported from Fuxian Lake and Xingyun Lake of Yunnan Province, Southwest China. V. vestibulatus differs from its allies by possessing modified spermathecal chaetae and thinner cylindrical penial sheaths. A. apeniatus is unique in the genus by having no penis. I. mesoprostatus is distinguishable from congeners by its prostate glands joining middle portion of atria and having concave, cone-shaped cuticular penial sheaths. Twenty-eight species of freshwater oligochaetes have hitherto been recorded from Yunnan Province, including five endemic species from three plateau lakes.

scholarly journals Impact of secondary salinisation on the structure and diversity of oligochaete communities

2020 ◽  
pp. 6
Author(s):  
Agnieszka Sowa ◽  
Mariola Krodkiewska
Keyword(s):  
Salinity Gradient ◽  
Significant Loss ◽  
Taxa Richness ◽  
Secondary Salinisation ◽  
Upper Silesian Coal Basin ◽  
Saline Rivers ◽  
Elevated Water ◽  
Inland Water Bodies ◽  
Potamothrix Hammoniensis ◽  
Freshwater Oligochaetes

Secondary salinisation has become one of the most important factors responsible for changes in the aquatic biota. Earlier research has focused on macroinvertebrates including oligochaetes in anthropogenically saline rivers and streams, but studies on oligochaetes in anthropogenically saline stagnant waters remain scarce. Therefore, this study was conducted to assess changes in the species composition as well as the abundance and biomass of oligochaete communities along a large salinity gradient in the anthropogenic inland water bodies located in the Upper Silesian Coal Basin (Southern Poland), which is one of the largest coal basins in Europe. Herein, a total of 27 oligochaete species including five alien species were assessed, namely, Potamothrix bavaricus, Potamothrix hammoniensis, Potamothrix moldaviensis, Psammoryctides albicola, and Psammoryctides barbatus. The results confirmed that the freshwater oligochaetes could tolerate elevated water salinity and showed highest densities and taxa richness in intermediate salinity. Moreover, the waters with the highest salinity had an extremely low number of oligochaete species. A salinity level above 2800 mg L−1 led to significant loss of diversity of the oligochaetes, and consequently, these habitats were colonized by halotolerant species, especially Paranais litoralis, whose abundance increased with increasing salinity gradient.

scholarly journals Global panorama of studies about freshwater oligochaetes: main trends and gaps

2018 ◽  
Vol 58 ◽  
pp. e20185838
Author(s):  
Luciana Falci Theza Rodrigues ◽  
Roberto Da Gama Alves
Keyword(s):  
United States ◽  
Molecular Biology ◽  
Southern Hemisphere ◽  
Web Of Science ◽  
The United States ◽  
Isi Web Of Science ◽  
Aquatic Oligochaetes ◽  
Wide Range ◽  
The World ◽  
Freshwater Oligochaetes

Freshwater oligochaetes have been studied over the years in a wide range of habitats around the world. To analyse the data published about freshwater oligochaetes in the 31 year period between 1985 and 2015, we searched for documents in the ISI Web of Science, Scopus and SciELO databases. A total of 979 works were evaluated from 184 periodicals. The United States is the country with the most publications about aquatic oligochaetes, followed by France, Germany and Italy. Works related to the ecology and ecotoxicology of these organisms are most abundant. Studies carried out in the laboratory are most frequent, consisting of investigations involving bioassays, morphology, genetics and molecular biology. The results also show that spring, cave water bodies and swamps have been studied less than any other aquatic habitat and that countries of the Southern Hemisphere are under-represented in the studies of aquatic oligochaetes. Hence, there is a need for increased efforts to fill in gaps in the knowledge about these oligochaetes.

Free Amino Acid Pools of Five Species of Freshwater Oligochaetes

1986 ◽  
Vol 43 (3) ◽  
pp. 600-607 ◽  
Author(s):  
R. L. Graney ◽  
T. J. Keilty ◽  
J. P. Giesy
Keyword(s):  
Amino Acid ◽  
Glutamic Acid ◽  
Free Amino ◽  
Lake Michigan ◽  
Tubifex Tubifex ◽  
Multivariate Statistical ◽  
Biochemical Processes ◽  
Limnodrilus Hoffmeisteri ◽  
Free Amino Acid Pools ◽  
Freshwater Oligochaetes

The concentrations of free amino acids (FAA) were measured in five species of freshwater Oligochaeta (Limnodrilus hoffmeisteri, Tubifex tubifex, Potamothrix moldaviensis, Potamothrix vejdovskyi, and Stylodrilus heringianus) collected from southeastern Lake Michigan. Alanine represented between 23.1 and 41.8% of the total FAA pool and was the most abundant amino acid in all species. Leucine, valine, and glutamic acid were, respectively, the next most abundant and had similar relative concentrations in all species except T. tubifex. In T. tubifex, glutamic acid, asparagine, and aspartate were the second, third, and fourth most abundant FAA, respectively. Relative to other freshwater invertebrates, the total FAA pool of Lake Michigan oligochaetes was small. Differences in innate physiological and/or biochemical processes, dietary intake, and reproductive status may have contributed to the quantitative and qualitative differences observed among species. Identification of individual oligochaetes to species was accomplished by multivariate statistical analysis of the FAA profiles. A potential exists for the application of this technique for identifying immature oligochaetes.

Genome size estimates for some oligochaete annelids

2002 ◽  
Vol 80 (8) ◽  
pp. 1485-1489 ◽  
Author(s):  
T Ryan Gregory ◽  
Paul D.N Hebert
Keyword(s):  
Life History ◽  
Genome Size ◽  
Dna Content ◽  
Life History Traits ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Interspecific Differences ◽  
Parthenogenetic Reproduction ◽  
Size Estimates ◽  
Freshwater Oligochaetes

Haploid genome sizes (C-values) were estimated for 12 species of freshwater oligochaetes (families Naididae and Tubificidae) and 15 species of earthworms (families Lumbricidae and Megascolecidae). Aquatic worms ranged in genome size from about 0.8 to 7.6 pg, while earthworms varied from approximately 0.4 to 1.2 pg. Interspecific differences in nuclear DNA content did not appear to be linked to variation in chromosome number, but there was evidence of cryptopolyploidy in the aquatic worms. No correlations were found between genome size and either body size or life-history traits, although there may be an association between higher DNA content and parthenogenetic reproduction in earthworms.

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