scholarly journals O03.2 The short-term immune impact of bacterial vaginosis treatment is unrelated to Lactobacillus spp. abundance

2021 ◽  
Author(s):  
E Armstrong ◽  
A Hemmerling ◽  
S Miller ◽  
K Burke ◽  
S Newmann ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Short Term ◽  
Lactobacillus Spp

scholarly journals Bacterial vaginosis: epidemiologic, clinical and diagnostic updates

2018 ◽  
Vol 32 (4) ◽  
Author(s):  
Giorgio Dirani ◽  
Silvia Zannoli ◽  
Maria Federica Pedna ◽  
Francesco Congestrì ◽  
Patrizia Farabegoli ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Current Status ◽  
Gardnerella Vaginalis ◽  
Vaginal Fluid ◽  
Molecular Approach ◽  
Candida Spp ◽  
Kappa Test ◽  
Lactobacillus Spp ◽  
Standard Techniques ◽  
Atopobium Vaginae

Background and aims. Bacterial vaginosis (BV) is one the more frequently identified genital syndrome among childbearing aged women. The basic condition that generates this condition is a modification in the vaginal microbiota. The aim of this paper is to briefly review the current status of the art of BV and to report the results of a pilot study performed with an innovative PCR based technique. Materials and Methods. 36 samples of vaginal fluid routinely submitted for the diagnosis of BV to the Unit of Microbiology – GRHL were comparatively evaluated by standard techniques and with the HP-Vaginiti e Vaginosi NLM kit that simultaneously detects in a quantitative way specific DNA from Candida (albicans, glabrata; krusei, tropicalis), Gardnerella vaginalis, Lactobacillus spp. and Atopobium vaginae. Results and conclusions. Candida spp. has been identified in 8 samples with culture and in 15 with the molecular test. 29 G. vaginalis were found by PCR whereas only in 7 samples a specific prescription for this microbe was present (of which 4 positive). A. vaginae has been identified in 20 samples by the molecular approach and Lactobacillus spp. was identified in 19 samples (by culture) and in 32 by PCR. The overall diagnosis of BV was made in 9 patients by standard techniques and in 7 by applying the molecular approach. (Cohen’s kappa test: 0,84). The findings of this study clearly demonstrate that the joint use of the routine culture- based techniques with the multiplex PCR methods amplifies by far the sensitivity of the overall diagnostic workflow of BV.

scholarly journals Bacterial vaginosis.

1991 ◽  
Vol 4 (4) ◽  
pp. 485-502 ◽  
Author(s):  
C A Spiegel
Keyword(s):  
Urinary Tract ◽  
Bacterial Vaginosis ◽  
Urinary Tract Infections ◽  
Anaerobic Bacteria ◽  
Fetal Loss ◽  
Reproductive Age ◽  
Genital Tract Infection ◽  
Increased Risk ◽  
Tract Infections ◽  
Lactobacillus Spp

Bacterial vaginosis (BV) is the most common of the vaginitides affecting women of reproductive age. It appears to be due to an alteration in the vaginal ecology by which Lactobacillus spp., the predominant organisms in the healthy vagina, are replaced by a mixed flora including Prevotella bivia, Prevotella disiens, Porphyromonas spp., Mobiluncus spp., and Peptostreptococcus spp. All of these organisms except Mobiluncus spp. are also members of the endogenous vaginal flora. While evidence from treatment trials does not support the notion that BV is sexually transmitted, recent studies have shown an increased risk associated with multiple sexual partners. It has also been suggested that the pathogenesis of BV may be similar to that of urinary tract infections, with the rectum serving as a reservoir for some BV-associated flora. The organisms associated with BV have also been recognized as agents of female upper genital tract infection, including pelvic inflammatory disease, and the syndrome BV has been associated with adverse outcome of pregnancy, including premature rupture of membranes, chorioamnionitis, and fetal loss; postpartum endometritis; cuff cellulitis; and urinary tract infections. The mechanisms by which the BV-associated flora causes the signs of BV are not well understood, but a role for H2O2-producing Lactobacillus spp. in protecting against colonization by catalase-negative anaerobic bacteria has been recognized. These and other aspects of BV are reviewed.

scholarly journals Assessment of vaginal microbiota in Brazilian women with and without bacterial vaginosis and comparison with Nugent score

2018 ◽  
Vol 12 (02) ◽  
pp. 127-136 ◽  
Author(s):  
Laura Maria Andrade Oliveira ◽  
Cláudio Galuppo Diniz ◽  
Aline Augusta Sampaio Fernandes ◽  
Daniele Maria Knupp Souza-Sotte ◽  
Michelle Cristine Ribeiro Freitas ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Inverse Correlation ◽  
Vaginal Microbiota ◽  
Gardnerella Vaginalis ◽  
Nugent Score ◽  
Gradient Gel Electrophoresis ◽  
Patient Groups ◽  
Lactobacillus Spp ◽  
Microbiota Structure

Introduction: Bacterial vaginosis (BV) is characterized by the depletion of Lactobacillus spp. population and increase of other species, especially Gardnerella vaginalis and Atopobium vaginae. This study aimed to investigate the vaginal microbiota structure of Brazilian women with and without BV according to Nugent Score and to assess the correlation among Nugent score and the quantification of BV-associated bacteria. Methodology: Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) assay was employed to characterize the vaginal microbiota structure. Quantification of Lactobacillus spp., G. vaginalis, A. vaginae, Mobiluncus sp. and M. hominis were determined by quantitative real-time PCR (qPCR). Results: Clustering by PCR-DGGE revealed differences in microbial structure of the different patient groups. Gardnerella vaginalis, A. vaginae, M. hominis and Mobiluncus sp. were detected at high loads in BV-associated microbiota. Quantification of Lactobacillus spp. showed an inverse correlation with Nugent score while the loads of G. vaginalis, A. vaginae, M. hominis and Mobiluncus sp. indicated a direct correlation with this method. Conclusions: Despite Nugent score is considered the gold standard for BV diagnosis, qPCR stands out as a useful tool for bacteria quantification and an alternative for BV diagnosis. Vaginal microbiota is a complex microbial community although there is a common core among BV and non-BV women. Investigation of vaginal microbiota structure may contribute to the development of tools for diagnosis improvement and therapeutic regimen optimization.

The Short-term Variability of Bacterial Vaginosis Diagnosed by Nugent Gram Stain Criteria Among Sexually Active Women in Rakai, Uganda

2011 ◽  
Vol 38 (2) ◽  
pp. 111-116 ◽  
Author(s):  
Marie E. Thoma ◽  
Ronald H. Gray ◽  
Noah Kiwanuka ◽  
Simon Aluma ◽  
Mei-Cheng Wang ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Sexually Active ◽  
Gram Stain ◽  
Short Term ◽  
Short Term Variability

scholarly journals Reactional changes in short-term levonorgestrel-releasing intrauterine system (lng-ius) use

2019 ◽  
Vol 65 (6) ◽  
pp. 857-863 ◽  
Author(s):  
Paulo César Giraldo ◽  
Thais Coelho de Souza ◽  
Guilherme Lindman Henrique ◽  
Ilza Monteiro ◽  
Rose Amaral ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Vaginal Discharge ◽  
Pap Smear ◽  
Cervical Mucus ◽  
Reproductive Age ◽  
Vaginal Candidiasis ◽  
Clinical Aspects ◽  
Vaginal Mucosa ◽  
Short Term ◽  
Quasi Experimental

SUMMARY OBJECTIVE: To evaluate endocervical and vaginal environment changes in women using a levonorgestrel-releasing intrauterine system (LNG-IUS). METHODS: A quasi-experimental study included sixty women who had an LNG-IUS inserted in the Family Planning Clinic of UNICAMP between April and November of 2016. Women in reproductive age, non-pregnant, without the use of antibiotics and contraceptives seeking for LNG-IUS insertion were selected for this study. All women were evaluated with regard to vaginal and endocervical pH, vaginal and endocervical Gram-stained bacterioscopy, and Pap-smear before and two months after LNG-IUS insertion. Clinical aspects such as cervical mucus, vaginal discharge, and cervical ectopy were also observed. RESULTS: After LNG-IUS insertion, there was an increase in the following parameters: endocervical pH>4.5 (p=0.02), endocervical neutrophil amount (p<0.0001), vaginal cytolysis (p=0.04). There was a decrease in vaginal discharge (p=0.01). No statistically significant changes were found in vaginal pH, neutrophils amount in the vaginal mucosa, vaginal discharge appearance, vaginal candidiasis, bacterial vaginosis, vaginal coccobacillary microbiota, cervical mucus appearance, or cervical ectopy size. CONCLUSIONS: Short-term LNG-IUS use did not increase vulvovaginal candidiasis or bacterial vaginosis, and led to diminished vaginal discharge. Notwithstanding, this device promoted reactional changes in the vaginal and endocervical environment, without modification on cervical ectopy size.

scholarly journals THE CLINICAL AND MICROBIOLOGICAL FEATURES OF BACTERIAL VAGINOSIS

2017 ◽  
pp. 15-18
Author(s):  
Yu. A. Lyzikova
Keyword(s):  
Bacterial Vaginosis ◽  
Sexually Transmitted Infections ◽  
Reproductive Function ◽  
Gardnerella Vaginalis ◽  
Control Group ◽  
Sexually Transmitted ◽  
Female Patients ◽  
Microbiological Examination ◽  
Lactobacillus Spp ◽  
Atopobium Vaginae

Aim : to determine the clinical and microbiological features of bacterial vaginosis on the basis of the microbiological examination, assessment of the cytokine status in female patients. Material and methods . The article presents the results of the complex clinical and microbiological examination of 86 female patients of the fertile age. 30 (34.88 ± 5.14 %) patients were diagnosed bacterial vaginosis on the basis of revelation and identification of DNA of Gardnerella vaginalis, Atopobium vaginae, Lactobacillus spp. and the total number of bacteria. The control group consisted of 56 (65.12 ± 5.14 %) patients without bacterial vaginosis. The work also presents the results of the microbiological analysis of the material obtained from the cervical canal and endometrium. All the patients underwent blood tests for detection of the inflammatory reaction - interleukins IL-1, IL-2, tumor necrosis factor (TNF-α), interferon (γ-IFN). Results . The prevalence of bacterial vaginosis among the patients of the reproductive age was 34.8 %. The pathology of the reproductive function was found with equal frequency in the patients of both the groups. Disorders of the immune status in favor of pro-inflammatory cytokines were not diagnosed. The clinical and laboratory criteria made it possible to diagnose bacterial vaginosis in 3.49 % of the patients, the use of PCR diagnosis - in 34.88 %. The concentration of lactobacillus spp. is reliably lower in the patients with bacterial vaginosis, than in the control group (p = 0.0085). As for the concentrations of Gardnerella vaginalis and Atopobium vaginae the groups do not significantly differ. Only 4 (13.33 ± 6.21 %) patients (χ = 5.51, p = 0.02) in the main group detected sexually transmitted infections, which should be taken into account while performing the diagnostic activities. Conclusion. The identification of DNA of certain kinds of microorganisms give an opportunity to assess the state of vaginal microcenosis and the degree of its malfunction even in the absence of clinical and other laboratory signs of bacterial vaginosis. The malfunction of the biocenosis of the genital tract is not associated with disorders of the reproductive function and does not lead to a change in the cytokine status. Patients with bacterial vaginosis are in the risk group for development of sexually transmitted infections, which should be taken into account while performing the diagnostic activities.

scholarly journals In Vitro Evaluation of Prebiotic Properties of a Commercial Artichoke Inflorescence Extract Revealed Bifidogenic Effects

Nutrients ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1552 ◽  
Author(s):  
Pieter Van den Abbeele ◽  
Jonas Ghyselinck ◽  
Massimo Marzorati ◽  
Agusti Villar ◽  
Andrea Zangara ◽  
...  
Keyword(s):  
Short Chain Fatty Acids ◽  
Gas Pressure ◽  
Microbial Metabolites ◽  
Short Term ◽  
Dry Extract ◽  
Health Related ◽  
Lactate Levels ◽  
Lactobacillus Spp ◽  
Measuring Ph

Background: Prebiotics used as a dietary supplement, stimulate health-related gut microbiota (e.g., bifidobacteria, lactobacilli, etc.). This study evaluated potential prebiotic effects of an artichoke aqueous dry extract (AADE) using in vitro gut model based on the Simulator of Human Intestinal Microbial Ecosystem (SHIME®). Methods: Short-term colonic fermentations (48 h) of AADE, fructo-oligosaccharides (FOS), and a blank were performed. Microbial metabolites were assessed at 0, 6, 24, and 48 h of colonic incubation via measuring pH, gas pressure, lactate, ammonium, and short-chain fatty acids (SCFAs) levels. Community composition was assessed via targeted qPCRs. Results: After 24 and 48 h of incubation, bifidobacteria levels increased 25-fold with AADE (p < 0.05) and >100-fold with FOS (p < 0.05) compared to blank. Lactobacillus spp. levels only tended to increase with AADE, whereas they increased 10-fold with FOS. At 6 h, pH decreased with AADE and FOS and remained stable until 48 h; however, gas pressure increased significantly till the end of study. Acetate, propionate, and total SCFA production increased significantly with both at all time-points. Lactate levels initially increased but branched SCFA and ammonium levels remained low till 48 h. Conclusion: AADE displayed prebiotic potential by exerting bifidogenic effects that stimulated production of health-related microbial metabolites, which is potentially due to inulin in AADE.

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