Notes sur la production, in vitro, de sporophores par Pholiota aurivella

The influence of light and other environmental conditions on the sporophore production was tested for Pholiota aurivella in the laboratory. Among different light sources tested, only sunlight, through the window, led to the production of complete sporophores in culture. The light stimulus must be given within the first 14 days following mycelium implantation. The number and size of sporophores increase with increasing nutrient concentration in the solid culture medium up to a level of 7% malt extract. Larger containers plugged with cotton are more favorable for the production of sporophores of normal size than smaller, hermetically closed containers.

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Temporary immersion biorreators: efficient technique for the propagation of the ‘Pircinque’ strawberry

Revista Brasileira de Fruticultura ◽

10.1590/0100-29452019102 ◽

2019 ◽

Vol 41 (1) ◽

Author(s):

Samila Silva Camargo ◽

Leo Rufato ◽

Maicon Magro ◽

André Luiz Kulkamp de Souza

Keyword(s):

Liquid Medium ◽

Culture Medium ◽

Culture Media ◽

Time Factors ◽

Efficient Technique ◽

Control Treatment ◽

Solid Culture ◽

Temporary Immersion ◽

Solid Culture Medium

Abstract The in vitro propagation technique via temporary immersion bioreactors is a tool that, through the culture in a liquid medium, allows an increase in the efficiency of seedling production. Several researches with the strawberry crop have shown greater efficiency of the system compared to the conventional process of micropropagation in solid medium. In this sense, the objective herein was to establish a protocol of multiplication and rooting of the ‘Pircinque’ strawberry, in temporary immersion bioreactors. Two distinct and independent studies were carried out, characterized by the multiplication and rooting stages of strawberry explants, newly introduced and registered in Brazil. Two culture media (MS and KNOP) were studied and, as a control treatment, the growth of the explants in solid culture medium was evaluated with the addition of 5 g L-1 of agar. Different immersion times of the culture medium were explored: five or eight times a day, for 15 minutes. The study was composed of the culture medium and immersion time factors, as well as the control (solid) treatment. It was verified that the use of temporary immersion bioreactors system is an efficient technique for the multiplication and rooting of explants of strawberry cv. Pircinque, when compared to the conventional method of micropropagation with the use of solid culture medium, making it possible to optimize the production of seedlings in biofactories. The MS liquid medium, in contact with explants of ‘Pircinque’ strawberry five times a day, increased the growth of the aerial part and the root system.

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Use of temporary immersion bioreactors and solid culture medium in the in vitro propagation of pear rootstocks

Acta Horticulturae ◽

10.17660/actahortic.2021.1303.17 ◽

2021 ◽

pp. 113-120

Author(s):

A.L. Arruda ◽

F.R. Nerbass ◽

A.A. Kretzschmar ◽

L. Rufato ◽

A.J. Posser ◽

...

Keyword(s):

In Vitro Propagation ◽

Culture Medium ◽

Solid Culture ◽

Temporary Immersion ◽

Solid Culture Medium

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Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Plants ◽

10.3390/plants10051010 ◽

2021 ◽

Vol 10 (5) ◽

pp. 1010

Author(s):

Eucario Mancilla-Álvarez ◽

Juan Antonio Pérez-Sato ◽

Rosalía Núñez-Pastrana ◽

José L. Spinoso-Castillo ◽

Jericó J. Bello-Bello

Keyword(s):

Culture Medium ◽

Solid Medium ◽

Survival Rates ◽

Shoot Multiplication ◽

Control Treatment ◽

Multiplication Rate ◽

Solid Culture ◽

Solid Culture Medium ◽

Semi Solid

Taro is important for its nutritional content, medicinal use, and bioethanol production. The aim of the present study was to compare different semi-automated bioreactors (SABs) during in vitro multiplication of C. esculenta. The SABs used were temporary immersion bioreactors (TIBs), SETIS™ bioreactors and ebb-and-flow bioreactors; semi-solid culture medium was used as a control treatment. At 30 d of culture, different developmental variables, determination of chlorophyll, stomatal content, and survival percentage during acclimatization were evaluated. SABs increased the shoot multiplication rate relative to the semi-solid medium; however, the SETIS™ bioreactor showed the highest shoot production, with 36 shoots per explant, and the highest chlorophyll content. The stomatal index was higher in the semi-solid medium compared to the SABs, while the percentage of closed stomata was higher in the SABs than in the semi-solid culture medium. The survival rate during acclimatization showed no differences among the culture systems assessed, obtaining survival rates higher than 99%. In conclusion, the SETIS™ bioreactor showed the highest multiplication rate; however, other bioreactor alternatives are available for semi-automation and cost reduction for micropropagation of C. esculenta.

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Effects of Preharvest Methyl Jasmonate Elicitation and Electrical Stimulation on Camptothecin Production by In Vitro Plants of Ophiorrhiza ridleyana Craib

Applied Sciences ◽

10.3390/app11104555 ◽

2021 ◽

Vol 11 (10) ◽

pp. 4555

Author(s):

Supakit Pisitpaibool ◽

Suchada Sukrong ◽

Kijchai Kanjanaprapakul ◽

Muenduen Phisalaphong

Keyword(s):

Tissue Culture ◽

Electrical Stimulation ◽

Methyl Jasmonate ◽

Culture Medium ◽

Solid Culture ◽

Direct Electric Current ◽

Solid Culture Medium ◽

The Mean ◽

Stems And Leaves

To enhance plant camptothecin (CPT) production in vitro, 5-month-old Ophiorrhiza ridleyana Craib plant cultures were treated with solutions of methyl jasmonate (MeJA) dissolved in ethanol, which were applied to the surface of the solid culture medium. It was demonstrated that the maximum CPT content in the tissue-cultured plants was achieved after 12 h elicitation with 50 µM MeJA. The mean CPT contents in roots and stems were 50.8 and 67.0 µg/g DW, respectively, which were approximately 1.8- and 2.6-fold higher, respectively, than those of the control. However, MeJA elicitation showed no significant effect on CPT accumulation in O. ridleyana leaves. Moreover, it was found that direct electric current (DC) stimulation also significantly increased CPT accumulation in O. ridleyana. The treatment with DC at 20 mA for 3 min of stimulation enhanced 3-fold the CPT content in roots, stems, and leaves to 41.9, 36.0 and 19.6 µg/g DW, respectively, which were approximately 1.5-, 1.7- and 1.4-fold higher, respectively, as compared to those of the control. The results demonstrate that preharvest treatment by MeJA elicitation and electrical stimulation can be beneficial for secondary metabolite production of CPT in tissue-culture plants of O. ridleyana.

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Immunopotentiating Activity of the Water-soluble Lignin Rich Fraction Prepared from LEM—The Extract of the Solid Culture Medium ofLentinus edodesMycelia—

Bioscience Biotechnology and Biochemistry ◽

10.1271/bbb.61.1909 ◽

1997 ◽

Vol 61 (11) ◽

pp. 1909-1912 ◽

Cited By ~ 29

Author(s):

Yoshiki Yamamoto ◽

Hiroyuki Shirono ◽

Keiko Kono ◽

Yasuhiro Ohashi

Keyword(s):

Culture Medium ◽

Water Soluble ◽

Solid Culture ◽

Solid Culture Medium ◽

Soluble Lignin

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Intracellular Biosynthesis of Fluorescent CdSe Quantum Dots inBacillus subtilis:A Strategy to Construct Signaling Bacterial Probes for Visually Detecting Interaction BetweenBacillus subtilisandStaphylococcus aureus

Microscopy and Microanalysis ◽

10.1017/s1431927615015548 ◽

2015 ◽

Vol 22 (1) ◽

pp. 13-21 ◽

Cited By ~ 5

Author(s):

Zheng-Yu Yan ◽

Xiao-Xia Ai ◽

Yi-Long Su ◽

Xin-Ying Liu ◽

Xiao-Hui Shan ◽

...

Keyword(s):

Quantum Dots ◽

Culture Medium ◽

Imaging Mass Spectrometry ◽

Cdse Quantum Dots ◽

Mass Spectrometry Detection ◽

New Paradigm ◽

Solid Culture ◽

Bacterial Interaction ◽

Solid Culture Medium ◽

Good Uniformity

AbstractIn this work, fluorescentBacillus subtilis(B. subtilis) cells were developed as probes for imaging applications and to explore behaviorial interaction betweenB. subtilisandStaphylococcus aureus(S. aureus). A novel biological strategy of coupling intracellular biochemical reactions for controllable biosynthesis of CdSe quantum dots by livingB. subtiliscells was demonstrated, through which highly luminant and photostable fluorescentB. subtiliscells were achieved with good uniformity. With the help of the obtained fluorescentB. subtiliscells probes,S. aureuscells responded to co-culturedB. subtilisand to aggregate. The degree of aggregation was calculated and nonlinearly fitted to a polynomial model. Systematic investigations of their interactions implied thatB. subtiliscells inhibit the growth of neighboringS. aureuscells, and this inhibition was affected by both the growth stage and the amount of surroundingB. subtiliscells. Compared to traditional methods of studying bacterial interaction between two species, such as solid culture medium colony observation and imaging mass spectrometry detection, the procedures were more simple, vivid, and photostable due to the efficient fluorescence intralabeling with less influence on the cells’ surface, which might provide a new paradigm for future visualization of microbial behavior.

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STUDIES ON CERTAIN GROWTH PARAMETERS OF DIFFERENT PROBIOTIC STRAINS

Journal of Engineering Studies and Research ◽

10.29081/jesr.v23i4.70 ◽

2017 ◽

Vol 23 (4) ◽

pp. 19-24

Author(s):

DUMITRA RĂDUCANU ◽

ANA-MARIA GEORGESCU

Keyword(s):

Nutrient Medium ◽

Growth And Development ◽

Culture Medium ◽

Growth Parameters ◽

Probiotic Bacteria ◽

Solid Culture ◽

Solid Culture Medium ◽

Probiotic Strains ◽

Direct Counting ◽

Better Than

The researchers found that probiotics contain microorganisms belonging to genus: Streptococcus, Lactobacillus, Bacillus, Aspergillus, Saccharomyces, Enterococcus, Pediococcus, enzymes (lactoperoxidase, gluconase, nonspecific enzymes) and rumen extracts. In this study, commercial probiotic bacteria known as "Linex" were used as samples. Cultural characteristics of these probiotic bacteria have been isolated and studied. It has been found that solid culture medium (nutritional gelose) favored the growth and development of bifidobacteria better than the liquid nutrient medium (nutrient broth). Thus, the number of bifidobacteria resulting from direct counting with Thoma chamber was of 7890 cells.

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Environmental conditions modulate the protein content and immunomodulatory activity of extracellular vesicles produced by the probiotic Propionibacterium freudenreichii

Applied and Environmental Microbiology ◽

10.1128/aem.02263-20 ◽

2020 ◽

Author(s):

Vinícius de Rezende Rodovalho ◽

Brenda Silva Rosa da Luz ◽

Aurélie Nicolas ◽

Fillipe Luiz Rosa do Carmo ◽

Julien Jardin ◽

...

Keyword(s):

Protein Content ◽

Extracellular Vesicles ◽

Environmental Conditions ◽

Culture Medium ◽

Surface Proteins ◽

Bioactive Molecules ◽

Propionibacterium Freudenreichii ◽

Therapeutic Applications ◽

Immunomodulatory Properties

Propionibacterium freudenreichii is a probiotic Gram-positive bacterium with promising immunomodulatory properties. It modulates regulatory cytokines, mitigates the inflammatory response in vitro and in vivo. These properties were initially attributed to specific bacterial surface proteins. Recently, we showed that extracellular vesicles (EVs) produced by P. freudenreichii CIRM-BIA129 mimic the immunomodulatory features of parent cells in vitro (i.e. modulating NF-κB transcription factor activity and IL-8 release) which underlies the role of EVs as mediators of the probiotic effects of the bacterium. The modulation of EV properties, and particularly of those with potential therapeutic applications such as the EVs produced by the probiotic P. freudenreichii, is one of the challenges in the field to achieve efficient yields with the desired optimal functionality. Here we evaluated whether the culture medium in which the bacteria are grown could be used as a lever to modulate the protein content and hence the properties of P. freudenreichii CIRM-BIA129 EVs. The physical, biochemical and functional properties of EVs produced from cells cultivated on laboratory Yeast Extract Lactate (YEL) medium and cow milk ultrafiltrate (UF) medium were compared. UF-derived EVs were more abundant, smaller in diameter and displayed more intense anti-inflammatory activity than YEL-derived EVs. Furthermore, the growth media modulated EV content in terms of both the identities and abundances of their protein cargos, suggesting different patterns of interaction with the host. Proteins involved in amino acid metabolism and central carbon metabolism were modulated, as were the key surface proteins mediating host-propionibacteria interactions. Importance Extracellular vesicles (EVs) are cellular membrane-derived nanosized particles that are produced by most cells in all three kingdoms of life. They play a pivotal role in cell-cell communication through their ability to transport bioactive molecules from donor to recipient cells. Bacterial EVs are important factors in host-microbe interactions. Recently we have shown that EVs produced by the probiotic P. freudenreichii exhibited immunomodulatory properties. We evaluate here the impact of environmental conditions, notably culture media, on P. freudenreichii EV production and function. We show that EVs display considerable differences in protein cargo and immunomodulation depending on the culture medium used. This work offers new perspectives for the development of probiotic EV-based molecular delivery systems, and reinforces the optimization of growth conditions as a tool to modulate the potential therapeutic applications of EVs.

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