Germination–lysis as a mechanism for biological control of Thielaviopsis basicola pathogenic on soybean

1976 ◽  
Vol 54 (13) ◽  
pp. 1499-1508 ◽  
Author(s):  
B. Sneh ◽  
B. F. Holdaway ◽  
G. R. Hooper ◽  
J. L. Lockwood
Keyword(s):  
Amino Acids ◽  
Green Plant ◽  
Water Soluble ◽  
Plant Residues ◽  
Thielaviopsis Basicola ◽  
Soybean Seedlings ◽  
Alfalfa Hay ◽  
Chloroform Methanol ◽  
Germ Tubes ◽  
Chlamydospore Germination

Amending soil with alfalfa hay or other dried, green plant residues stimulated chlamydospore germination of Thielaviopsis basicola. Within 3 to 6 days the germ tubes lysed resulting in the decline in viable chlamydospores and of disease severity of soybean seedlings planted in amended soil. When chlamydospores were added immediately after the amendment, maximum germination was recorded after 2 days. The number of spores with germ tubes declined to zero after 6 days as a result of lysis. Incubation of alfalfa in soil for 1 or more days before chlamydospores were added reduced or nullified the effect of the amendment. Water-soluble substances from alfalfa hay, including carbohydrates, amino acids, and organic acids, were more stimulatory and caused greater decline in chlamydospore population than ether or chloroform-methanol-soluble substances.

Interactions of water-soluble zinc porphyrin with amino acids

1988 ◽  
Vol 153 (4) ◽  
pp. 199-200 ◽  
Author(s):  
E. Mikros ◽  
A. Gaudemer ◽  
R. Pasternack
Keyword(s):  
Amino Acids ◽  
Water Soluble ◽  
Zinc Porphyrin ◽  
Soluble Zinc

scholarly journals Incorporation of alternative amino acids into cyanophycin by different cyanophycin synthetases heterologously expressed in Corynebacterium glutamicum

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ramona Wördemann ◽  
Lars Wiefel ◽  
Volker F. Wendisch ◽  
Alexander Steinbüchel
Keyword(s):  
Amino Acids ◽  
Dry Weight ◽  
Growth Conditions ◽  
Amino Acid Biosynthesis ◽  
Water Soluble ◽  
Lysine Content ◽  
Polyaspartic Acid ◽  
Acid Biosynthesis ◽  
Cyanophycin Synthetase ◽  
Potential Precursor

AbstractCyanophycin (multi-l-arginyl-poly-l-aspartic acid; also known as cyanophycin grana peptide [CGP]) is a biopolymer that could be used in various fields, for example, as a potential precursor for the synthesis of polyaspartic acid or for the production of CGP-derived dipeptides. To extend the applications of this polymer, it is therefore of interest to synthesize CGP with different compositions. A recent re-evaluation of the CGP synthesis in C. glutamicum has shown that C. glutamicum is a potentially interesting microorganism for CGP synthesis with a high content of alternative amino acids. This study shows that the amount of alternative amino acids can be increased by using mutants of C. glutamicum with altered amino acid biosynthesis. With the DM1729 mutant, the lysine content in the polymer could be increased up to 33.5 mol%. Furthermore, an ornithine content of up to 12.6 mol% was achieved with ORN2(Pgdh4). How much water-soluble or insoluble CGP is synthesized is strongly related to the used cyanophycin synthetase. CphADh synthesizes soluble CGP exclusively. However, soluble CGP could also be isolated from cells expressing CphA6308Δ1 or CphA6308Δ1_C595S in addition to insoluble CGP in all examined strains. The point mutation in CphA6308Δ1_C595S partially resulted in a higher lysine content. In addition, the CGP content could be increased to 36% of the cell dry weight under optimizing growth conditions in C. glutamicum ATCC13032. All known alternative major amino acids for CGP synthesis (lysine, ornithine, citrulline, and glutamic acid) could be incorporated into CGP in C. glutamicum.

scholarly journals A Water-Soluble Copper-Immobilized Covalent Organic Framework Functioning as an “OFF–ON” Fluorescent Sensor for Amino Acids

2021 ◽  
Author(s):  
Lamiaa Reda Ahmed ◽  
Ahmed F. M. EL-Mahdy ◽  
Cheng-Tang Pan ◽  
Shiao-Wei Kuo
Keyword(s):  
Amino Acids ◽  
Fluorescent Sensor ◽  
Water Soluble ◽  
One Pot ◽  
Covalent Organic Framework ◽  
The One ◽  
Organic Framework

In this paper, we describe the construction of a new fluorescent hydroxyl- and hydrazone-based covalent organic framework (TFPB-DHTH COF) through the one-pot polycondensation of 1,3,5-tris(4-formylphenyl)benzene (TFPB) and 2,5-dihydroxyterephthalohydrazide (DHTH) under...

scholarly journals Erratum to: Antibacterial Effects of Amino Acids-grafted Water-soluble Chitosan against Drug-resistant Bacteria

2021 ◽  
Vol 26 (3) ◽  
pp. 501-501
Author(s):  
Jun-Ho Kim ◽  
Nam-Hong Kim ◽  
Eun-Ji Kim ◽  
Ji Ho Kim ◽  
Min-Young Lee ◽  
...  
Keyword(s):  
Amino Acids ◽  
Water Soluble ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Antibacterial Effects ◽  
Drug Resistant Bacteria

scholarly journals Nutritional Composition,α-Glucosidase Inhibitory and Antioxidant Activities ofOphiopogon japonicusTubers

2015 ◽  
Vol 2015 ◽  
pp. 1-7
Author(s):  
Yancui Wang ◽  
Feng Liu ◽  
Zongsuo Liang
Keyword(s):  
Amino Acids ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Nutritional Composition ◽  
Total Phenolic ◽  
Ophiopogon Japonicus ◽  
Nutritional Compounds ◽  
Chloroform Methanol ◽  
Phenolic And Flavonoid

Ophiopogon japonicustubers have been widely used as food and traditional Chinese medicine in China. However, their nutritional composition has not been fully reported yet. This study aimed to analyze the nutritional composition ofO. japonicustubers. Theα-glucosidase inhibitory and antioxidant activities of the extracts obtained fromO. japonicustubers were also evaluated byin vitroassays. The results indicated thatO. japonicustubers are rich in carbohydrates, proteins, minerals, and amino acids. Among four extracts, the n-butanol fraction (nBF) and chloroform/methanol extract (CME) ofO. japonicustubers had high amounts of total phenolic and flavonoid contents and exhibited goodα-glucosidase inhibitory and antioxidant activities. Theα-glucosidase inhibition of nBF was higher than acarbose. Overall,O. japonicustubers are full of nutritional compounds and have goodα-glucosidase inhibitory and antioxidant activities.

scholarly journals Water-Soluble Blue Fluorescent Nonconjugated Polymer Dots from Hyaluronic Acid and Hydrophobic Amino Acids

ACS Omega ◽  
2021 ◽  
Author(s):  
Deep S. Bhattacharya ◽  
Aishwarya Bapat ◽  
Denis Svechkarev ◽  
Aaron M. Mohs
Keyword(s):  
Amino Acids ◽  
Hyaluronic Acid ◽  
Water Soluble ◽  
Polymer Dots ◽  
Hydrophobic Amino Acids

scholarly journals Cyclodextrin Multicomponent Complexes: Pharmaceutical Applications

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1099
Author(s):  
Virginia Aiassa ◽  
Claudia Garnero ◽  
Marcela R. Longhi ◽  
Ariana Zoppi
Keyword(s):  
Amino Acids ◽  
Pharmaceutical Industry ◽  
Drug Effects ◽  
Water Soluble ◽  
Therapeutic Drug ◽  
Water Soluble Polymers ◽  
Soluble Polymers ◽  
Pharmaceutical Applications ◽  
Auxiliary Substance ◽  
Acids And Bases

Cyclodextrins (CDs) are naturally available water-soluble cyclic oligosaccharides widely used as carriers in the pharmaceutical industry for their ability to modulate several properties of drugs through the formation of drug–CD complexes. The addition of an auxiliary substance when forming multicomponent complexes is an adequate strategy to enhance complexation efficiency and to facilitate the therapeutic applicability of different drugs. This review discusses multicomponent complexation using amino acids; organic acids and bases; and water-soluble polymers as auxiliary excipients. Special attention is given to improved properties by including information on the solubility, dissolution, permeation, stability and bioavailability of several relevant drugs. In addition, the use of multicomponent CD complexes to enhance therapeutic drug effects is summarized.

scholarly journals Determination of gangliosides as 2,4-dinitrophenylhydrazides by high-performance liquid chromatography

1986 ◽  
Vol 235 (3) ◽  
pp. 755-761 ◽  
Author(s):  
K Miyazaki ◽  
N Okamura ◽  
Y Kishimoto ◽  
Y C Lee
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Normal Phase ◽  
Water Soluble ◽  
Acid System ◽  
Total Lipids ◽  
Acetylneuraminic Acid ◽  
Chloroform Methanol ◽  
Phase Column

A specific, sensitive and easily performed method for the determination of gangliosides in tissue was developed. After removal of water-soluble compounds, total lipids were extracted from tissue and then treated with 2,4-dinitrophenylhydrazine hydrochloride and dicyclohexylcarbodi-imide in dimethylformamide at 0 degrees C to form ganglioside hydrazides. After removal of excess reagents by column chromatography on silicic acid, the ganglioside 2,4-dinitrophenylhydrazides were eluted from the column and analysed by h.p.l.c. with the use of a silica-gel normal-phase column eluted with an isocratic chloroform/methanol/water/acetic acid system. The addition of CaCl2 improved the separation of GM3 ganglioside containing N-acetylneuraminic acid from that containing N-glycollylneuraminic acid. 2,4-Dinitrophenylhydrazide peaks were measured by the absorbance at 342 nm. Quantification of GM3, GM2, GM1, GD1a, GD1b, GT1b and LM1 gangliosides was linear in a range 0.02-1.6 nmol. GM4, GD3, GT1a and GQ1b gangliosides also yielded distinct peaks, although the range of linearity was not examined. This method was applied to the analysis of the total lipids of rat brain and hepatocytes.

scholarly journals Effects of Proteolytic and Lipolytic Enzyme Supplementations on Lipolysis and Proteolysis Characteristics of White Cheeses

Foods ◽  
2018 ◽  
Vol 7 (8) ◽  
pp. 125 ◽  
Author(s):  
Oya Berkay Karaca ◽  
Mehmet Güven
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Titratable Acidity ◽  
Water Soluble ◽  
Soluble Nitrogen ◽  
Lipolytic Enzyme ◽  
White Cheese ◽  
Amino Acid Contents ◽  
Mineral Levels

Effects of proteolytic (Neutrase, Bacillus subtilis-originate, 0.20 (P1) and 0.40 g 100 L−1 (P2)) and lipolytic (Piccantase A, Mucor miehei-originated, 0.05 (L1) and 0.10 g 100 L−1 (L2)) enzyme supplementations to cheese milk on lipolysis and proteolysis characteristics of 90-day ripened cheese samples were investigated in this study. While enzyme supplementation did not have significant effects on titratable acidity, fat and protease-peptone nitrogen ratios of cheese samples, dry matter, salt, protein, water soluble nitrogen, 12% trichloroacetic acid soluble nitrogen ratio (TCA-SN), 5% phosphotungstic acid soluble nitrogen (PTA-SN), casein nitrogen ratios, penetrometer value, total free fatty acids (TFFA) and total free amino acids (TFAA) were significantly influenced by enzyme supplementations. Individual free amino acids (15 of them) were also determined. Free amino acid contents of enzyme-supplemented cheeses were higher than the control cheese and the values increased in all cheese samples with the progress of ripening (p < 0.05). The highest amino acids in all periods of ripening were identified as glutamic acid, lysine, proline and aspartic acid. The major (Ca, P, Na, K, Mg) and minor (Zn, Fe, Cu, Mn) mineral levels of cheeses decreased with the progress of ripening and the effects of enzyme supplementations on these attributes (except for magnesium and manganese) were found to be significant (p < 0.01). As to conclude, enzyme supplementations increased proteolysis and lipolysis and accelerated ripening and thus reduced ripening durations. Especially the enzyme ratios in P1 and L1 cheeses were found to be suitable for reducing the ripening period in White cheese without any adverse effects.

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