Citrus tissue culture: regulation of stylar abscission in excised pistils

1980 ◽  
Vol 58 (11) ◽  
pp. 1257-1261 ◽  
Author(s):  
John W. Einset ◽  
Anne Cheng ◽  
Hamid Elhag
Keyword(s):  
Tissue Culture ◽  
Cell Division ◽  
Dichlorophenoxyacetic Acid ◽  
Navel Orange ◽  
Test Medium ◽  
Fresh Weight ◽  
Valencia Orange ◽  
Washington Navel ◽  
Citrus Tissue Culture ◽  
2,4 Dichlorophenoxyacetic Acid

Lemon pistil explants were obtained by cutting just above the region of the hypogynous disc (A type explant) or at the base of the pistil (B type explant) and cultured on test medium containing Murashige and Skoog salts, 50 g sucrose/L, 100 mg myo-inositol/L, 5 mg thiamine–HCl/L, and 0.5 mg kinetin/L, plus or minus supplements. Under appropriate conditions an abscission zone formed and styles abscised after 6–8 days of culture; in the field stylar abscission occurred 12–15 days postanthesis. Abscission in A type explants was markedly inhibited by 9 μM 2,4-dichlorophenoxyacetic acid but was unaffected by indole-3-acetic, 1-naphthaleneacetic, gibberellic, abscisic, caffeic, or p-coumaric acids. The response to 2,4-dichlorophenoxyacetic acid was reduced in B type explants. In an atmosphere containing 35–200 ppm ethylene, cell division occurred in the zone of stylar abscission producing a proliferating callus, and the content of cellulase increased from 0.6 to 53.7 enzyme units/g fresh weight compared with fresh explants. Stylar abscission was inhibited by 2,4-dichlorophenoxyacetic acid in A type explants of Washington navel orange, Valencia orange, and mandarin pistils, but not of grapefruit pistils. B type explants of Washington navel orange and mandarin pistils were less responsive to 2,4-dichlorophenoxyacetic acid.

scholarly journals Induksi kalus Artemisia vulgaris L. dengan Pemberian Beberapa Konsentrasi 2,4-Dichlorophenoxyacetic Acid(2,4-D)

2015 ◽  
Vol 4 (4) ◽  
pp. 216
Author(s):  
Nazhira - Fadhilah ◽  
Zozy Aneloi Noli ◽  
Suwirmen - Suwirmen
Keyword(s):  
Plant Physiology ◽  
Tissue Culture ◽  
Effective Concentration ◽  
Dichlorophenoxyacetic Acid ◽  
Artemisia Vulgaris ◽  
Fresh Weight ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Physiology Laboratory

The research about callus induction Artemisia vulgaris L. by giving several concentration 2,4-Dichlorophenoxyacetic acid (2,4-D), has been done from May to August 2015 in Plant Physiology Laboratory and Tissue Culture, Department of Biology, Faculty of Mathematics and Natural Science, University of Andalas. The aim of this studywas found the effective concentration of 2,4-D to induce callus of A. vulgaris. The research used Completely Randomized Design (CRD) with 7 treatments and 4 replications. The treatments were : without 2,4-D (control); 0.25 mg/L 2,4-D; 0.50 mg/L 2,4-D; 0.75 mg/L 2,4-D; 1.00 mg/L 2,4-D; 1.25 mg/L 2,4-D; 1.5 mg/L 2,4-D. The result showed that 0.25-1,5 mg/L 2,4-D were able induction callus of A. vulgaris, withcompactuntilthefriabletexture, color ofthe resultingcallusisyellowish green, brownish-green, yellow-brown, whiteyellowishandgreenishwhite. 2,4-D 1.5mg/Lwas the best concentrationto increase fresh weight of callus.

scholarly journals Callus Induction of Aerides odorata Lour. by Adding 2,4 Dichlorophenoxyacetic Acid (2,4-D)

2019 ◽  
Vol 7 (2) ◽  
pp. 109
Author(s):  
Azharia Khalida ◽  
Suwirmen Suwirmen ◽  
Zozy Aneloi Noli
Keyword(s):  
Plant Physiology ◽  
Tissue Culture ◽  
Natural Science ◽  
Callus Induction ◽  
Dichlorophenoxyacetic Acid ◽  
Fresh Weight ◽  
Greenish Yellow ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
2,4 Dichlorophenoxyacetic Acid

The research about callus induction of Aerides odorata L. by adding 2,4 Dichlorophenoxyacetic Acid (2,4-D), has been done from August to October 2018 in Plant Physiology and Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Natural Science, Andalas University, Padang. The aim of this research was found the effective consentration of 2,4-D to induce somatic embryo of A.odorata. The research used Completely Randomized Design (CRD) with 5 treatments and 6 replications. The treatments were: 0 mg/L 2,4-D; 0,25 mg/L 2,4-D; 0,5 mg/L 2,4-D; 0,75 mg/L 2,4-D; 1 mg/L 2,4-D. The result showed that the treatmeant were able induction callus of A.odorata, with compact until the friable texture, color of the resulting callus is yellowish green and greenish yellow. 2,4-D 1 mg/L was the best concentration to increase fresh weight of callus.

scholarly journals The mode of action of 2,4-D in counteracting the elongation of carrot cells grown in culture

1980 ◽  
Vol 45 (1) ◽  
pp. 257-268
Author(s):  
C.W. Lloyd ◽  
S.B. Lowe ◽  
G.W. Peace
Keyword(s):  
Electron Microscopy ◽  
Cell Division ◽  
Dichlorophenoxyacetic Acid ◽  
Mitogenic Effect ◽  
Suspension Cells ◽  
Carrot Cells ◽  
Cytoplasmic Microtubules ◽  
Time Periods ◽  
Per Se ◽  
2,4 Dichlorophenoxyacetic Acid

The growth regulator 2,4-D (2,4-dichlorophenoxyacetic acid) has been used to investigate the inter-relationship between cell elongation and cell division in carrot suspension cells. Maintained in 1 mg/l. 2,4-D, dividing populations of cells remain spheroidal and in clusters. But when subcultured into lower levels or zero, 2,4-D they increasingly elongate at the expense of division. Over the range of 0 to 1.0 mg/l, 2,4-D, elongation and division are therefore inversely related. However, by suppressing the mitogenic effect with FUdR it can be shown that cells do elongate in 1 mg/l. 2,4-D—a concentration which otherwise produces dividing, spheroidal cells. This indicates that mitogenc levels of 2,4-D do not perturb structures which support cellular elongation. This conclusion is confirmed by immuno- and electron-microscopy which show that development of elaborate arrays of cytoplasmic microtubules is unaffected by 1 mg/l. 2,4-D when FUdR is present. It is concluded that over the time periods under study here, 2,4-D regulates cell size (and shape) by stimulating growing cells to enter the division cycle and not by inhibiting elongation per se.

scholarly journals Comparison of foliar fertilizers and growth regulators on pre-harvest drop and fruit quality of ‘Thompson Navel’ orange

2016 ◽  
Vol 1 (1) ◽  
Author(s):  
Yaser Mollapur ◽  
Seied Mehdi Miri ◽  
Ebrahim Hadavi
Keyword(s):  
Salicylic Acid ◽  
Citric Acid ◽  
Growth Regulators ◽  
Fruit Quality ◽  
Calcium Nitrate ◽  
Dichlorophenoxyacetic Acid ◽  
Navel Orange ◽  
Fruit Decay ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Old Trees

AbstractAn investigation was carried out to determine the ability of foliar fertilizers and growth regulators to prevent pre-harvest drop and enhance navel orange fruit quality. Fifteen year old trees of the Thompson Navel orange variety were sprayed with aqueous solutions of one of the following: methanol (0.13%), calcium nitrate (0.25%), zinc sulfate (1%) + urea (0.5%), fermented sugar- cane extract (2.8%), salicylic acid (1 or 3 mM), citric acid (5 mM), or 2,4-dichlorophenoxyacetic acid (2,4-D) (0.002%). Results indicated that foliar treatment with 2,4-D reduced fruit drop (2.6%) as compared to the control (15.2%). Fruit decay was delayed and flavor improved by 5 mM citric acid. Weight loss was reduced after 3mM salicylic acid applica- tion, while methanol (0.13%), salicylic acid (1 and 3 mM) and citric acid (5 mM) caused a delay in fruit coloring.

scholarly journals Callus Formation on Endosperm from Immature and Mature Fruits of Barringtonia Racemosa

2019 ◽  
Vol 7 (4.14) ◽  
pp. 107
Author(s):  
D S M Soder ◽  
D N A A Khalid ◽  
A Saleh ◽  
F Pardi ◽  
N J Sidik
Keyword(s):  
Callus Induction ◽  
Callus Formation ◽  
Chemical Compounds ◽  
Dichlorophenoxyacetic Acid ◽  
Maturity Stage ◽  
Ms Medium ◽  
Fresh Weight ◽  
Pharmacological Activities ◽  
Friable Callus ◽  
2,4 Dichlorophenoxyacetic Acid

Barringtonia racemosa is mangroves type of plant which had been extensively utilized in conventional practices for relieving ailments of pain and inflammation. Many studies have been done on ethnobotanical profiles, pharmacological activities and chemical compounds in Barringtonia racemosa. However, there is a limited study on callogenesis of this plant particularly from different maturity stage of fruits. The present study is to identify the callogenesis of Barringtonia racemosa from endosperm explants of immature and mature fruits in MS medium supplemented with different concentrations of hormones 2,4-Dichlorophenoxyacetic acid (2,4-D) (0, 0.5, 1.0, 1.5 and 2.0 mg/L) and Kinetin (KIN) (0, 0.5, 1.0, 1.5 and 2.0 mg/L). The optimum hormone combination was found in callus grown on endosperm of immature fruits in MS medium supplemented with 1.5 mg/L 2,4-D and 1.0 mg/L KIN. It was also found that the callus in this treatment grew profusely with highest fresh weight (0.513 ± 0.022 g), 100% callus induction and friable callus texture. The callus fresh weight on endosperm explants was higher in immature fruits compared to mature fruits for all the hormone combinations. Therefore, callogenesis were found more efficient from endosperm explant of immature fruits in Barringtonia racemosa species.   

scholarly journals The Effects of 2,4-Dichlorophenoxyacetic Acid and α-Naphthaleneacetic Acid on Biomass Increment, Rhizogenesis and Somatic Embryogenesis of Suspension-cultured Dinh Lang Cells [Polyscias fruticosa (L.) Harms]

2021 ◽  
Author(s):  
T.T.B. Phuong ◽  
V.P. Trung ◽  
N.H. An ◽  
N.D. Tuan ◽  
P.T.T. Nguyen
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryos ◽  
Bioactive Compound ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Fresh Weight ◽  
Triterpenoid Saponins ◽  
Cell Biomass ◽  
2,4 Dichlorophenoxyacetic Acid

Background: Dinh Lang [Polyscias fruticosa (L.) Harms] is a medicinal plant widely grown in Vietnam, with proven note-worthy health benefits. However, Dinh Lang’s amounts of triterpenoid saponins could not meet the need of the pharmaceutical industry. Thus, this study’s purpose is to figure out the optimal condition for raising Dinh Lang’s cell biomass, rhizogenesis and somatic embryogenesis to provide materials for bioactive compound productions. Methods: Different 2,4-dichlorophenoxyacetic acid and α-naphthaleneacetic acid concentrations (0.5, 1.0, 1.5 and 2.0 mg/L) were examined to determine the best amount of each plant growth regulator for raising cells’ biomass, rhizogenesis and somatic embryogenesis. In each treatment, two grams of eight-week-old calli were cultured in 50 mL of liquid MS medium. Result: It is demonstrated by the results that liquid MS medium containing 1.5 mg/L α-naphthaleneacetic acid has the capacity of producing the highest numbers of somatic embryos (489 embryos per flask) and rooted cells (259.5 cells per flask), while the fresh weight of cells cultured in the medium given 1.5 mg/L 2,4-dichlorophenoxyacetic acid reached its peak of 5.7 g.

Reduction of fertile regenerants from protoplasts of Triticum tauschii (Coss.) Schmal.

2000 ◽  
Vol 48 (4) ◽  
pp. 501
Author(s):  
Shoukat Afshar-Sterle ◽  
James F. Kollmorgen ◽  
Geoffrey B. Fincher
Keyword(s):  
Cell Division ◽  
Somatic Embryos ◽  
Dichlorophenoxyacetic Acid ◽  
Cell Aggregates ◽  
Ms Medium ◽  
Triticum Tauschii ◽  
Cell Divisions ◽  
Aegilops Squarrosa ◽  
Half Strength ◽  
2,4 Dichlorophenoxyacetic Acid

Four suspension cell lines generated from two accessions of Triticum tauschii (Coss.) Schmal. (Aegilops squarrosa, 2n = 2x = 14, DD genome) were used to develop an efficient protocol for producing fertile regenerants from protoplasts. Protoplasts were isolated from each cell line by incubating fine cell aggregates (<500 µm in diameter) in a solution containing 3% Cellulase ‘Onozuka’ RS, 0.5% Macerozyme R10 and 0.2% Pectolyase Y23. Cell division occurred when the protoplasts were cultured at a density of 1.0–1.5 x 106 protoplasts mL–1 in half-strength MS medium supplemented with 1.1 mg L–1 2,4-dichlorophenoxyacetic acid (2,4-D), 0.6 M glucose and 1.2% agarose. The first cell divisions were observed after 5–7 days. Cell colonies were observed after 14 days and these grew quickly into large clumps when transferred to half-strength MS medium supplemented with 2.2 mg L-1 2,4-D, 30 g L–1 sucrose and solidified with 0.25% Phytagel. The colonies produced somatic embryos within 21–28 days of transfer to this medium. Somatic embryos were transferred to hormone-free MS medium for regeneration into plantlets. Although many regenerants produced shrivelled seeds, 9 of 16 were fertile and produced normal seeds.

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