scholarly journals Induksi kalus Artemisia vulgaris L. dengan Pemberian Beberapa Konsentrasi 2,4-Dichlorophenoxyacetic Acid(2,4-D)

2015 ◽  
Vol 4 (4) ◽  
pp. 216
Author(s):  
Nazhira - Fadhilah ◽  
Zozy Aneloi Noli ◽  
Suwirmen - Suwirmen
Keyword(s):  
Plant Physiology ◽  
Tissue Culture ◽  
Effective Concentration ◽  
Dichlorophenoxyacetic Acid ◽  
Artemisia Vulgaris ◽  
Fresh Weight ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Physiology Laboratory

The research about callus induction Artemisia vulgaris L. by giving several concentration 2,4-Dichlorophenoxyacetic acid (2,4-D), has been done from May to August 2015 in Plant Physiology Laboratory and Tissue Culture, Department of Biology, Faculty of Mathematics and Natural Science, University of Andalas. The aim of this studywas found the effective concentration of 2,4-D to induce callus of A. vulgaris. The research used Completely Randomized Design (CRD) with 7 treatments and 4 replications. The treatments were : without 2,4-D (control); 0.25 mg/L 2,4-D; 0.50 mg/L 2,4-D; 0.75 mg/L 2,4-D; 1.00 mg/L 2,4-D; 1.25 mg/L 2,4-D; 1.5 mg/L 2,4-D. The result showed that 0.25-1,5 mg/L 2,4-D were able induction callus of A. vulgaris, withcompactuntilthefriabletexture, color ofthe resultingcallusisyellowish green, brownish-green, yellow-brown, whiteyellowishandgreenishwhite. 2,4-D 1.5mg/Lwas the best concentrationto increase fresh weight of callus.

scholarly journals Callus Induction of Aerides odorata Lour. by Adding 2,4 Dichlorophenoxyacetic Acid (2,4-D)

2019 ◽  
Vol 7 (2) ◽  
pp. 109
Author(s):  
Azharia Khalida ◽  
Suwirmen Suwirmen ◽  
Zozy Aneloi Noli
Keyword(s):  
Plant Physiology ◽  
Tissue Culture ◽  
Natural Science ◽  
Callus Induction ◽  
Dichlorophenoxyacetic Acid ◽  
Fresh Weight ◽  
Greenish Yellow ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
2,4 Dichlorophenoxyacetic Acid

The research about callus induction of Aerides odorata L. by adding 2,4 Dichlorophenoxyacetic Acid (2,4-D), has been done from August to October 2018 in Plant Physiology and Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Natural Science, Andalas University, Padang. The aim of this research was found the effective consentration of 2,4-D to induce somatic embryo of A.odorata. The research used Completely Randomized Design (CRD) with 5 treatments and 6 replications. The treatments were: 0 mg/L 2,4-D; 0,25 mg/L 2,4-D; 0,5 mg/L 2,4-D; 0,75 mg/L 2,4-D; 1 mg/L 2,4-D. The result showed that the treatmeant were able induction callus of A.odorata, with compact until the friable texture, color of the resulting callus is yellowish green and greenish yellow. 2,4-D 1 mg/L was the best concentration to increase fresh weight of callus.

scholarly journals Pengaruh Naphthalene Asam Asetat (NAA) pada Pertumbuhan Akar Pisang Raja Kinalun Secara In Vitro

2018 ◽  
Vol 5 (1) ◽  
pp. 1
Author(s):  
Rahmi Rini Dwi Putri ◽  
Suwirmen Suwirmen ◽  
Nasril Nasir
Keyword(s):  
Plant Physiology ◽  
Tissue Culture ◽  
Root Growth ◽  
Natural Sciences ◽  
Effective Concentration ◽  
Randomized Design ◽  
Effective Doses ◽  
Completely Randomized Design

The influence of Naphthalene Acetate Acid (NAA) on the in vitro root growth of banana Raja Kinalun was carried out from April to September 2015 in The Laboratories of Plant Physiology and Tissue Culture, Faculty of Mathematics and Natural Sciences, University of Andalas, Padang. The aim of  this study was to find the effective concentration of NAA for initiation of root. This study used a Completely Randomized Design (CRD) with four treatments and three replications. The treatments were: without NAA (control); NAA 1 ppm, NAA 2 ppm and NAA 3 ppm. The result showed that the effective doses were NAA 1 and 2 ppm for each number of roots growth.

Citrus tissue culture: regulation of stylar abscission in excised pistils

1980 ◽  
Vol 58 (11) ◽  
pp. 1257-1261 ◽  
Author(s):  
John W. Einset ◽  
Anne Cheng ◽  
Hamid Elhag
Keyword(s):  
Tissue Culture ◽  
Cell Division ◽  
Dichlorophenoxyacetic Acid ◽  
Navel Orange ◽  
Test Medium ◽  
Fresh Weight ◽  
Valencia Orange ◽  
Washington Navel ◽  
Citrus Tissue Culture ◽  
2,4 Dichlorophenoxyacetic Acid

Lemon pistil explants were obtained by cutting just above the region of the hypogynous disc (A type explant) or at the base of the pistil (B type explant) and cultured on test medium containing Murashige and Skoog salts, 50 g sucrose/L, 100 mg myo-inositol/L, 5 mg thiamine–HCl/L, and 0.5 mg kinetin/L, plus or minus supplements. Under appropriate conditions an abscission zone formed and styles abscised after 6–8 days of culture; in the field stylar abscission occurred 12–15 days postanthesis. Abscission in A type explants was markedly inhibited by 9 μM 2,4-dichlorophenoxyacetic acid but was unaffected by indole-3-acetic, 1-naphthaleneacetic, gibberellic, abscisic, caffeic, or p-coumaric acids. The response to 2,4-dichlorophenoxyacetic acid was reduced in B type explants. In an atmosphere containing 35–200 ppm ethylene, cell division occurred in the zone of stylar abscission producing a proliferating callus, and the content of cellulase increased from 0.6 to 53.7 enzyme units/g fresh weight compared with fresh explants. Stylar abscission was inhibited by 2,4-dichlorophenoxyacetic acid in A type explants of Washington navel orange, Valencia orange, and mandarin pistils, but not of grapefruit pistils. B type explants of Washington navel orange and mandarin pistils were less responsive to 2,4-dichlorophenoxyacetic acid.

scholarly journals Viability of Cyperus rotundus L. tubers after application of herbicide in pre- and postemergence

2020 ◽  
Vol 87 ◽  
Author(s):  
Ana Ligia Giraldeli ◽  
André Felipe Moreira Silva ◽  
Felipe Carrara de Brito ◽  
Robson Josadaque Nogueira de Lima ◽  
Beatriz Sizilio dos Santos ◽  
...  
Keyword(s):  
Active Ingredient ◽  
Dry Matter ◽  
Visual Control ◽  
Cyperus Rotundus ◽  
Dichlorophenoxyacetic Acid ◽  
Purple Nutsedge ◽  
Chemical Management ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
2,4 Dichlorophenoxyacetic Acid

ABSTRACT Chemical management is the most widely adopted technique to control weeds in sugarcane crops. Purple nutsedge (Cyperus rotundus L.) is among the most difficult species to control. Herbicides need to be absorbed and translocated in a sufficient amount for the tuber chain to be effective. Thus, this study aimed to evaluate the effectiveness of the herbicides sulfentrazone, diclosulam, imazapic, imazapyr, halosulfuron, ethoxysulfuron, monosodium methyl arsenate (MSMA), and 2,4-dichlorophenoxyacetic acid (2,4-D) in controlling C. rotundus and reducing the viability of its tubers. The experiment was carried out in a greenhouse in a completely randomized design, with nine treatments and four replications. The herbicides sulfentrazone (800 g active ingredient – ai·ha?1), diclosulam (193.17 g ai·ha?1), imazapic (133 g ai·ha?1), and imazapyr (500 g acid equivalent – ae·ha?1) were applied in preemergence at five days after planting the tubers, while halosulfuron (112.5 g ai·ha?1), ethoxysulfuron (135 g ai·ha?1), MSMA (1,975 g ai·ha?1), and 2,4-D (1,340 g ae·ha?1) were applied in postemergence (4 to 5 leaves). The parameters visual control, shoot dry matter, number of tubers and bulbs, weight of tubers + bulbs, number of epigeal manifestations, and tuber viability were analyzed. The herbicides diclosulam, halosulfuron, and ethoxysulfuron provided 100% control of the shoot at 90 days after application (DAA). All herbicides reduced the number of bulbs, weight of tubers + bulbs, and shoot dry matter. The herbicides sulfentrazone, imazapic, halosulfuron, ethoxysulfuron, and MSMA provided the highest reduction in tuber viability.

scholarly journals PERTUMBUHAN KALUS DAUN MELON (Cucumis melo) VARIETAS MAI 119 DENGAN PEMBERIAN BAP (BENZYL AMINO PURINE) DAN 2,4-D (2,4 DICHLOROPHENOXYACETIC ACID)

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Nining Intan Toharah ◽  
Dwi Soelistya Dyah Jekti ◽  
Lalu Zulkifli
Keyword(s):  
Growth Regulators ◽  
Callus Induction ◽  
Cucumis Melo ◽  
Callus Formation ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Benzyl Amino Purine

This study aims to determine the concentration of growth regulators BAP and   2,4-D which have the highest effect in stimulating the formation of callus melon plants (Cucumis melo) Mai 119 variety. Completely randomized design (CRD) was used in this research. Media used on callus induction was MS medium with addition of several concentration of BAP  (0 mg/L, 1 mg/L, 2 mg/L, 3 mg/L) and 2,4-D (0 mg/L, 1 mg/L, 2 mg/L, 3 mg/L) either alone or in a combination of both. Parameters measured were the time appearing of callus, callus diameter, callus texture, and callus color. Anova followed by Tukey's test was used to the analyse of time appearing of callus. Data of callus diameter was analyzed using Kruskal Wallis test followed by Mann-Whitney test. In the analysis of parameter related to the callus texture and callus color, descriptive test were used. The results showed that there were differences in the effect of growth regulators on the callus formation. The fastest callus induction and the largest diameter of callus were obtained on media with concentration of 2 mg/L BAP and 3 mg/L BAP.Keywords: BAP (benzyl amino purine), 2,4-D (2,4-dichlorophenoxyacetic acid), callus induction, melon (Cucumis melo) varieties Mai 119

scholarly journals Effect of Different Substrate on Growth and Yield of Button Mushroom

2013 ◽  
Vol 5 (2) ◽  
pp. 177-180 ◽  
Author(s):  
MJ Uddin ◽  
S Haque ◽  
ME Haque ◽  
S Bilkis ◽  
AK Biswas
Keyword(s):  
Tissue Culture ◽  
Growth And Yield ◽  
Fruiting Bodies ◽  
Fresh Weight ◽  
Paddy Straw ◽  
Button Mushroom ◽  
Training Centre ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
And Training

An experiment was conducted at the Mushroom growth house and Tissue Culture Laboratory, Horticulture Demonstration and Training Centre (HDTC), Kewatkhali, Mymensingh during February to May, 2006 to investigate the effect of different substrate on growth and yield of Button mushroom (Agaricus biporus). The substrates which were used in the experiment were wheat: paddy (1:1) straw compost, paddy straw compost and decomposed cowdung. The parameters observed in first and second flush were number of primordia, number of fruiting bodies and fresh weight of mushroom. Data were statistically analyzed following Completely Randomized Design (CRD). Data revealed that different substrate significantly affected the production of number of primordia, number of fruiting bodies and fresh weight. In both the first and second flush, all the three observed parameters were obtained highest in wheat: paddy straw compost and the lowest were in decomposed cowdung.DOI: http://dx.doi.org/10.3329/jesnr.v5i2.14810 J. Environ. Sci. & Natural Resources, 5(2): 177-180 2012

scholarly journals Induksi Kalus Tanaman Puspa (Schima wallichii (DC.) Korth) dengan Penambahan Beberapa Konsentrasi Benzyl Amino Purin (BAP) dan 2,4-Diklorofenoksiasetat (2,4-D)

2019 ◽  
Vol 7 (1) ◽  
pp. 1
Author(s):  
Husri Meli ◽  
Zozy Aneloi Noli ◽  
Suwirmen Suwirmen
Keyword(s):  
Plant Physiology ◽  
Tissue Culture ◽  
Natural Science ◽  
Callus Induction ◽  
Design Method ◽  
Randomized Design ◽  
Schima Wallichii ◽  
Completely Randomized Design ◽  
Department Faculty ◽  
Benzyl Amino Purine

The research about Callus Induction of Puspa (Schima wallichii (DC.) Korth) with several concentrations addition of Benzyl Amino Purine (BAP) and 2,4-Dichlorophenoxyacetid acid  (2,4-D) had been done from October until November 2016 at Plant Physiology dan Tissue Culture Laboratory, Biology Department, Faculty of Mathematics and Natural Science, Andalas University. The aim of this researh is to get the combination of 2,4-D and BAP to induce the best callus of Schima wallichii. This research used a Completely Randomized Design Method with 10 treatments and 3 replications. The result showed that combination of 2 ppm 2,4-D + 0,75 ppm BAP was the best concentration to induce callus of Schima wallichii.

scholarly journals Pengaruh Konsentrasi Giberelin (GA3) terhadap Pertumbuhan Kailan (Brassica oleracea L. Var alboglabra) pada Berbagai Media Tanam dengan Hidroponik Wick System

2018 ◽  
Vol 6 (2) ◽  
pp. 63
Author(s):  
Asih Maharani ◽  
Suwirmen Suwirmen ◽  
Zozy Aneloi Noli
Keyword(s):  
Plant Physiology ◽  
Brassica Oleracea ◽  
Natural Science ◽  
Growth And Yield ◽  
Randomized Design ◽  
Chinese Kale ◽  
Accelerate Growth ◽  
Completely Randomized Design ◽  
Physiology Laboratory ◽  
Brassica Oleracea L

Effect of giberelin (GA3) concentration on growth and yield of Chinese kale (Brassica oleracea L. Var alboglabra) in various medium using hydroponic wick system . This research conducted from April until July 2016 in Wire House and Plant Physiology Laboratory, Department of Biology, Faculty of  Mathematics and Natural Science, Andalas University. The purposes of this research were to obtain GA3 concentration and planting medium that accelerate growth and yield of Chinese kale and determine the interaction between GA3 and planting medium to the gowth and yield of Chinese kale planted using hydroponics wick system. This research used Completely Randomized Design (CRD) in factorial with three replications, as the first factor were concentration of GA3 (0 ppm, 20 ppm, 40 ppm and 60 ppm) and the second factor were medium (zeolite, sand, husk and sawdust). The best result in accelerated the growth of Chinese kale was the treatment at the concentration of 60 ppm GA3 and zeolite was suitable medium to Chinese kale growth. This research found the interaction between GA3 and medium on the length of the roots Chinese kale used hydroponic wick system.

scholarly journals Aplikasi Pupuk Organik Cair Menggunakan Bioaktivator Mikroorganisme Indigenous HPPB Untuk Pertumbuhan Desmodium heterophyllum pada Tanah Bekas Tambang Batu Kapur PT. Semen Padang

2018 ◽  
Vol 5 (1) ◽  
pp. 6
Author(s):  
Rizqa Zidna Chairafahmi ◽  
Suwirmen Suwirmen ◽  
Zozy Aneloi Noli
Keyword(s):  
Plant Physiology ◽  
Land Cover ◽  
Organic Fertilizers ◽  
Soil Science ◽  
Fresh Weight ◽  
Indigenous Microorganism ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
C 30

The research about application of liquid organic fertilizers with indigenous microorganism bioactivator of HPPB for growth of Desmodium heterophyllum on limestone mined land at PT. Semen Padang, had been conducted from May to August 2015 in Nursery and Reforestation, Laboratory of Plant Physiology Department of Biology, and the Laboratory of Soil Science Faculty of Agriculture, Andalas University, Padang. The research aimed to determine the effect and concentration of Liquid Organic Fertilizers (LOF) by using indigenous microorganism bioactivator originated from HPPB in limestone mined land ofPT. Semen Padang. This study used Completely Randomized Design (CRD) with five treatments and four replications. The treatments were control without LOF (A), 10% LOF (B), 20% LOF (C), 30% LOF (D) and 40% LOF (E). The results showed that 10% LOF was the best concentration to increase the number of leaves (36,44), percentage of land cover (6,71%) and fresh weight of plants (5,15g).

scholarly journals The Growth of Shoot Cutting of Ant-nest Plant (Myrmecodia pendens Merr. & L.M. Perry) that Planted in Various Planting Medium

2019 ◽  
Vol 7 (2) ◽  
pp. 91
Author(s):  
Suci Rahmadani Putri ◽  
Suwirmen Suwirmen ◽  
Zozy Aneloi Noli
Keyword(s):  
Plant Physiology ◽  
Natural Science ◽  
Root Length ◽  
Coconut Fiber ◽  
Randomized Design ◽  
Ant Nest ◽  
Completely Randomized Design ◽  
Physiology Laboratory

The research about The Growth of Shoot Cutting of Ant-nest Plant (Myrmecodia pendens Merr. & L.M. Perry) that Planted in various planting medium was held from May until August 2018 in Greenhouse and Plant Physiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Science, Andalas University, Padang. The aim of this research was to find out the effects of various planting medium to the growth of shoot cutting Ant-nest Plant. This research used Completely Randomized Design (CRD) with five treatments (sand, husk charcoal, media moss, coconut fiber and fern’s root) and six replications. The results showed that shoot cutting that planted in sand, husk charcoal and fern had a highest life percentage (100%). Shoot cutting that planted in coconut fiber showed a highest height accretion. Shoot cutting that planted in media moss showed the highest root amount, longest root length and containing clorophyl level.

Sign in / Sign up

Export Citation Format

Share Document