Honey as a bioindicator of environmental organochlorine insecticides contamination

Honey is a suitable matrix for the evaluation of environmental contaminants including organochlorine insecticides. The present study was conducted to evaluate residues of fifteen organochlorine insecticides in honey samples of unifloral and multifloral origins from Dir, Pakistan. Honey samples (5 g each) were extracted with GC grade organic solvents and then subjected to Rotary Evaporator till dryness. The extracts were then mixed with n-Hexane (5 ml) and purified through Column Chromatography. Purified extracts (1μl each) were processed through Gas Chromatograph coupled with Electron Capture Detector (GC-ECD) for identification and quantification of the insecticides. Of the 15 insecticides tested, 46.7% were detected while 53.3% were not detected in the honey samples. Heptachlor was the most prevalent insecticide with a mean level of 0.0018 mg/kg detected in 80% of the samples followed by β-HCH with a mean level of 0.0016 mg/kg detected in 71.4% of the honey samples. Honey samples from Acacia modesta Wall. were 100% positive for Heptachlor with a mean level of 0.0048 mg/kg followed by β-HCH with a mean level of 0.003 mg/kg and frequency of 83.3%. Minimum levels of the tested insecticides were detected in the unifloral honey from Ziziphus jujuba Mill. Methoxychlor, Endosulfan, Endrin and metabolites of DDT were not detected in the studied honey samples. Some of the tested insecticides are banned in Pakistan but are still detected in honey samples indicating their use in the study area. The detected levels of all insecticides were below the Maximum Residue Levels (MRLs) and safe for consumers. However, the levels detected can cause mortality in insect fauna. The use of banned insecticides is one of the main factors responsible for the declining populations of important insect pollinators including honeybees.

Selection of Restoration Material for Abies koreana Based on Its Genetic Diversity on Mt. Hallasan

The restoration of damaged or disrupted forests with genetically appropriate restoration planting material that can adapt to future environmental conditions will ensure the conservation of forest genetic resources. Abies koreana is endemic to the Republic of Korea, with declining populations under current environmental changes. In this study, we examined the genetic diversity of its largest population growing on Mt. Hallasan to determine the sampling size of planting material from the population that will ensure 95% coverage of alleles in the population. We evaluated the genetic diversity and spatial genetic structure of three subpopulations of A. koreana on Mt. Hallasan. A total of 456 samples were evaluated using 10 microsatellites. The observed heterozygosity and expected heterozygosity were 0.538 and 0.614 at the population level, respectively. The differences among the subpopulations accounted for 4% of the total variance. Intervals between individuals of the sample to be extracted were based on the two-target distance (5 and 10 m) inferred from the spatial genetic structure. Through random sampling methods considering the target distance, we showed that genetic diversity can be captured by obtaining at least 35 individuals in the population of A. koreana on Mt. Hallasan.

Avian Use of Dairy Farm Ponds and Landowners’ Perceptions of Their Management for Wildlife Conservation

Farm ponds are among the last remaining lentic wetland habitats in human-dominated agricultural and suburban landscapes. However, their wildlife value and farmers’ willingness to maintain them for the conservation of regionally declining biodiversity are often both poorly documented. The objectives of this study of 61 dairy farm ponds located in Southern Quebec (Canada) were to (1) describe their biophysical features and birds’ use, (2) determine habitat and landscape characteristics that influence the bird community, and (3) assess the willingness of farmers to support wildlife use. The studied ponds were small (0.17 ha) and had rectangular shape with rocky/muddy steeply slopes (21°), surrounded by 3 m wide riparian strips and pastures, hayfields, and fallows. They were located about 300 m from farmhouses, buildings, streams, and adjacent ponds. A total of 1963 individuals belonging to 46 bird species were observed. The abundance of all bird species, of species with declining populations, and of crop damaging species were positively related to the area of fallow land and to the width of riparian strips; the areas of cereals and of mixed-wood forest had a negative influence. Only two habitat variables had influence on species richness: the width of riparian strips (+) and the distance to the closest farm buildings (–). Most pond landowners (>80%) were in favour of increasing wildlife use if they were given access to associated financial support and logistical assistance. Wider riparian strips and adjacent uncultivated field margins are recommended.

Re-orientating the Experience of a Small Town

<p>An experience by definition can imply that it leaves an impression. It can be how people experience our small towns that influence its viability and success. Small towns in Aotearoa face aging populations, growing social issues and tend to lack economic viability. Current statistics show population growth in our large cities and declining populations in our regions impacting the urban landscape and the experience of our regional towns. So, how can designing from an experiential approach make a difference to town life? A traditional approach to changing the experience of towns and cities is through consultation, a production of a master plan and then staging the design over time. Consider what could be gained if we used on-the-ground experience to inform the structure our towns? If we intensely engage with the ‘on the ground experience’ of the town, looking at how the town structures experience, we should be able to identify opportunities that could re-orientate how people interact with the town. These opportunities or potential design alterations, could reshape the way that the town is structured and therefore inform the development of a master plan or long term plan. Through an experience-based design approach the opportunities that can be identified from this method are often not visible from other techniques such as top down mapping and other common forms of data collection. Through a combination of; an understanding of the experience of the town, its history, influences, though various forms of mapping and the understanding the viability of proposed design alterations we could strategically transform the experience. Therefore, through an experiential design approach and understanding how our small towns work in Aotearoa; can we focus design actions in the public environment to develop potential future change?</p>

Re-orientating the Experience of a Small Town

<p>An experience by definition can imply that it leaves an impression. It can be how people experience our small towns that influence its viability and success. Small towns in Aotearoa face aging populations, growing social issues and tend to lack economic viability. Current statistics show population growth in our large cities and declining populations in our regions impacting the urban landscape and the experience of our regional towns. So, how can designing from an experiential approach make a difference to town life? A traditional approach to changing the experience of towns and cities is through consultation, a production of a master plan and then staging the design over time. Consider what could be gained if we used on-the-ground experience to inform the structure our towns? If we intensely engage with the ‘on the ground experience’ of the town, looking at how the town structures experience, we should be able to identify opportunities that could re-orientate how people interact with the town. These opportunities or potential design alterations, could reshape the way that the town is structured and therefore inform the development of a master plan or long term plan. Through an experience-based design approach the opportunities that can be identified from this method are often not visible from other techniques such as top down mapping and other common forms of data collection. Through a combination of; an understanding of the experience of the town, its history, influences, though various forms of mapping and the understanding the viability of proposed design alterations we could strategically transform the experience. Therefore, through an experiential design approach and understanding how our small towns work in Aotearoa; can we focus design actions in the public environment to develop potential future change?</p>

Experiences and Lessons Learned from Stakeholder Engagement in Transitioning to Use of Community Energy Business Entity - In the Case of Remote Islands, Nagasaki

Five years have passed since the liberalization of retail electricity sales in Japan. Consumers are now free to choose their own power company and can make a contract with power companies that better suit their lifestyles. As a result of this liberalization, many electric power companies have come up with their own plans. In this trend, emerging community energy business entities that aim for "local production for local consumption" have recently begun to increase. This paper attempts to identify the strategies and challenges from the experience of stakeholder engagement in community energy initiatives. Stakeholder engagement in this context is a form of community engagement used to develop a viable agenda and implementation plan in line with the interests and needs of key stakeholders and constituencies. It is important to seek strategies to involve more stakeholders in decision-making when building new energy systems within a community, especially for in the development of remote island areas with rapidly declining populations. This paper focuses the case study of Nagasaki Prefecture, which has the largest number of remote island municipalities in Japan. Based on semi-structured interviews and document analysis, this research identifies the scope and engagement strategy of the community energy initiatives, and discuss lessons learned and challenges from the experience of energy stakeholder engagement. In the context of remote islands, a geographically isolated and resource-constrained area, both theoretical and practical issues are presented. This paper also presents options and outreach issues to encourage the broader range of stakeholder participation than just energy projects. Keywords: Community Energy Business Entities, Stakeholder Engagement, Community Engagement, Community Energy, Remote Islands, Nagasaki

Primer design of D-loop region for wild population genetics of Rusa timorensis in Indonesia

Rusa timorensis (Javan deer) is endemic wildlife in Indonesia and is estimated at less than 10.000 individuals with continuously declining populations due to habitat loss and illegal hunting in the wild. This declining low population indicates a greater risk of extinction. Unfortunately, the genetic information of the wild Javan deer population for conservation management strategies still lacks data due to challenging sampling in the wild. Most recent studies were analysing the breeding populations outside Indonesia. Here, we propose the primer design of the D-loop genetic marker to determine the genetic population of wild Javan deer. We used metadata analysis of genetic sequences and new samples from five wild populations to design the specific primer of the D-loop region of the wild Javan deer in Indonesia. We used software, i.e.., Primer3 to design the primers, BLAST for specificity and Oligo Analyzer™ Tool for efficiency of the primer. The Annealing temperature optimisation started with pre-denaturation at 94 °C followed by 35 cycles of denaturation at 95°C; 51-56°C annealing for each one degree’s different per PCR treatment; and 72°C extensions. We successfully designed a specific primer (RL-3.1a) to amplify 235 bp of the D-loop region at 52°C annealing’s temperature.

Unexpected Discovery of Thelypteris palustris (Thelypteridaceae) in Sicily (Italy): Morphological, Ecological Analysis and Habitat Characterization

Thelypteris palustris Schott (Thelypteridaceae), known as “marsh fern”, is infrequent in the Mediterranean area. The occurrence of this species is known for almost all the Italian regions (except for Sardinia and Sicily), but with rare and declining populations. During floristic fieldwork on the Sicilian wetlands, a new unknown population was found. The aim of this paper is to analyze the morphological traits of the species, as well as its ecological features and the floristic composition of the plant communities where it lives. According to IUCN guidelines, here we provide the regional assessment (Sicily) of T. palustris. To analyze its morphological features, many living plants were examined, with particular attention to the spore structure. A total of 179 plots (110 species) and 34 pools were sampled. Our results highlight the relic character of the species which is at the southernmost border of its distribution range. The micro-morphological investigations on the spores show that the Sicilian population belongs to the subsp. palustris. The floristic analysis confirms the clear dominance of perennial temperate-cold zones Eurasian taxa. Finally, a new association, Thelypterido palustris-Caricetum paniculatae, within the Caricion gracilis alliance (Phragmito-Magnocaricetea class) is described.

Autumn migration of Ospreys from two distinct populations in Poland reveals partial migratory divide

Background Long-term ringing and telemetry studies show that the Osprey (Pandion haliaetus) is a broad-front migrant following different migratory flyways, depending on the geographical location of their breeding populations. We have investigated two distinct and declining populations of Osprey in Poland, separated by only a few hundred kilometres, and hypothesised they may exhibit two different migration routes. We followed mortality causes, comparing them between migration and stationary phases of annual cycle, as well as between two distinct populations. Methods Nineteen Ospreys, both juveniles and adults, were equipped with GPS loggers in 2017–2020 in two populations in western and eastern Poland and followed on their autumn migration. We calculated the distance they covered on the migration, number of stopover days, migration duration, daily distances covered and departure dates to compare them between age and sex classes and between the eastern and western populations. Results Ospreys from the western and eastern populations showed a partial migratory divide. While the first migrated through a western flyway, the second followed a central flyway, resulting in crossing the Mediterranean Sea in distant passes that affected the distance covered. Annual mortality reached at least 67% in juveniles and at least 57% in adults. Conclusions We showed that two distinct Osprey populations in Poland revealed a partial migratory divide, with one covering greater distances over sea and deserts over the central flyway. This might affect individual survival rates and contribute to a steeper decline in one of the populations. In order for this to be confirmed, more individuals still have to be followed.