Evaluation of recombinant protein superoxide dismutase of Haemophilus parasuis strain SH0165 as vaccine candidate in a mouse model

Haemophilus parasuis can cause a severe membrane inflammation disorder. It has been documented that superoxide dismutase (SOD) is a potential target to treat systemic inflammatory diseases. Therefore, we constructed an experimental H. parasuis subunit vaccine SOD and determined the protective efficacy of SOD using a lethal dose challenge against H. parasuis serovar 4 strain MD0322 and serovar 5 strain SH0165 in a mouse model. The results demonstrated that SOD could induce a strong humoral immune response in mice and provide significant immunoprotection efficacy against a lethal dose of H. parasuis serovar 4 strain MD0322 or serovar 5 strain SH0165 challenge. IgG subtype analysis indicated SOD protein could trigger a bias toward a Th1-type immune response and induce the proliferation of splenocytes and secretion of IL-2 and IFN-γ of splenocytes. In addition, serum in mice from the SOD-immunized group could inhibit the growth of strain MD0322 and strain SH0165 in the whole-blood killing bacteria assay. This is the first report that immunization of mice with SOD protein could provide protective effect against a lethal dose of H. parasuis serovar 4 and serovar 5 challenge in mice, which may provide a novel approach against heterogeneous serovar infection of H. parasuis in future.

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Virulence of Listeria monocytogenes Propagated in NaCl Containing Media at 4, 25 and 37°C

Journal of Food Protection ◽

10.4315/0362-028x-57.6.475 ◽

1994 ◽

Vol 57 (6) ◽

pp. 475-478 ◽

Cited By ~ 9

Author(s):

ERIC R. MYERS ◽

SCOTT E. MARTIN

Keyword(s):

Superoxide Dismutase ◽

Listeria Monocytogenes ◽

Sodium Chloride ◽

Mouse Model ◽

Virulence Factor ◽

Growth Temperature ◽

Lethal Dose ◽

Listeriolysin O ◽

Sod Activity ◽

Approximate Lethal Dose

Virulence, as determined in a mouse model, and virulence factor activities of catalase (CA), superoxide dismutase (SOD) and listeriolysin O (LLO), was examined in Listeria monocytogenes 10403S. Cells were propagated in media containing various concentrations of sodium chloride (NaCl) at 4, 25 and 37°C. Strain 10403S exhibited significant increases in CA activity and LLO when grown in media containing 428 mM of NaCl at 37°C. The CA activities at 4 and 25°C were significantly less, and the cells exhibited similar increases and decreases as cells grown at 37°C. When comparing the growth temperatures, the CA activity decreased as the growth temperature decreased. The SOD activity was significantly increased only when cells were propagated in media containing either 428 or 1,112 mM of NaCl. The SOD activity increased as the growth temperature decreased. No LLO activity was detected when cells were grown at 4 and 25°C. The production of these enzymes appeared to be thermoregulated. In addition, approximate lethal dose (ALD50) values were determined after intragastric (i.g.) and intraperitoneal (i.p.) infection. Each method of infection indicated that LLO was required for virulence, while growth in salt containing media, growth at 4°C, or the production of higher levels of CA, SOD and LLO did not appear to influence the virulence of L. monocytogenes.

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Reversal by citrovorum factor of methotrexate-induced suppression of cell-mediated and humoral immune response in mouse model systems

Biochemical Pharmacology ◽

10.1016/0006-2952(82)90033-8 ◽

1982 ◽

Vol 31 (7) ◽

pp. 1387-1392 ◽

Cited By ~ 2

Author(s):

Dennis Bogyo ◽

M.Jane Ehrke ◽

Enrico Mihich

Keyword(s):

Immune Response ◽

Mouse Model ◽

Humoral Immune Response ◽

Model Systems ◽

Humoral Immune

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The Live Attenuated Actinobacillus pleuropneumoniae Triple-Deletion Mutant ΔapxICΔapxIICΔapxIV-ORF1Strain, SLW05, Immunizes Pigs against Lethal Challenge with Haemophilus parasuis

Clinical and Vaccine Immunology ◽

10.1128/cvi.00458-12 ◽

2012 ◽

Vol 20 (2) ◽

pp. 134-139 ◽

Cited By ~ 11

Author(s):

Shulin Fu ◽

Jiwen Ou ◽

Minmin Zhang ◽

Juan Xu ◽

Huazhen Liu ◽

...

Keyword(s):

Deletion Mutant ◽

Lethal Dose ◽

Interleukin 2 ◽

Actinobacillus Pleuropneumoniae ◽

Economic Losses ◽

Respiratory Pathogens ◽

Haemophilus Parasuis ◽

Lethal Challenge ◽

Content Type ◽

Novel Approach

ABSTRACTHaemophilus parasuisandActinobacillus pleuropneumoniaeboth belong to the familyPasteurellaceaeand are major respiratory pathogens that cause large economic losses in the pig industry worldwide. We previously constructed an attenuatedA. pleuropneumoniaeserovar 1 live vaccine prototype, SLW05 (ΔapxICΔapxIICΔapxIV-ORF1), which is able to produce nontoxic but immunogenic ApxIA, ApxIIA, and ApxIVA. This triple-deletion mutant strain was shown to elicit protective immunity against virulentA. pleuropneumoniae. In the present study, we investigated whether immunization with SLW05 could also protect against lethal challenge with virulentH. parasuisSH0165 (serovar 5) or MD0322 (serovar 4). The SLW05 strain was found to elicit a strong humoral antibody response in pigs and to confer significant protection against challenge with a lethal dose ofH. parasuisSH0165 or MD0322. IgG subtype analysis revealed that SLW05 induces a bias toward a Th1-type immune response and stimulates interleukin 2 (IL-2) and gamma interferon (IFN-γ) production. Moreover, antisera from SLW05-vaccinated pigs efficiently inhibited bothA. pleuropneumoniaeandH. parasuisgrowth in a whole-blood assay. This is the first report that a live attenuatedA. pleuropneumoniaevaccine with SLW05 can protect against lethalH. parasuisinfection, which provides a novel approach for developing an attenuatedH. parasuisvaccine.

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Intraspleen Delivery of a DNA Vaccine Coding for Superoxide Dismutase (SOD) of Brucella abortus Induces SOD-Specific CD4+ and CD8+ T Cells

Infection and Immunity ◽

10.1128/iai.72.4.2081-2087.2004 ◽

2004 ◽

Vol 72 (4) ◽

pp. 2081-2087 ◽

Cited By ~ 41

Author(s):

Carola Muñoz-Montesino ◽

Edilia Andrews ◽

Rodolfo Rivers ◽

Andrés González-Smith ◽

Gustavo Moraga-Cid ◽

...

Keyword(s):

Immune Response ◽

T Cells ◽

Superoxide Dismutase ◽

T Cell ◽

Cytotoxic Activity ◽

Brucella Abortus ◽

Plasmid Dna ◽

Interleukin 4 ◽

Similar Amount ◽

Ifn Γ

ABSTRACT In the development of vaccines capable of providing immunity against brucellosis, Cu-Zn superoxide dismutase (SOD) has been demonstrated to be one of the protective immunogens of Brucella abortus. In an earlier study, we provided strong evidence that intramuscular injection with a plasmid DNA carrying the SOD gene (pcDNA-SOD) was able to induce a protective immune response. The present study was designed to characterize T-cell immune responses after an intraspleen (i.s.) vaccination of BALB/c mice with pcDNA-SOD. Animals vaccinated with pcDNA-SOD did not develop SOD-specific antibodies, at least until week 4 after immunization (the end of the experiment), and in vitro stimulation of their splenocytes with either recombinant Cu-Zn SOD or crude Brucella protein induced the secretion of gamma interferon (IFN-γ), but not interleukin-4, and elicited the induction of cytotoxic-T-lymphocyte activity. Upon analyzing the SOD-specific T-cell responses, the pcDNA-SOD vaccination was found to be stimulating both CD4+- and CD8+-T-cell populations. However, only the CD4+ population was able to produce IFN-γ and only the CD8+ population was able to induce cytotoxic activity. Nevertheless, although i.s. route vaccination induces a significant level of protection in BALB/c mice against challenge with the virulent B. abortus strain 2308, vaccination by the intramuscular route with a similar amount of plasmid DNA does not protect. Based on these results, we conclude that i.s. immunization with pcDNA-SOD vaccine efficiently induced a Th1 type of immune response and a protective response that could be related to IFN-γ production and cytotoxic activity against infected cells by SOD-specific CD4+ and CD8+ T cells, respectively.

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Comparison of gE/gI- and TK/gE/gI-Gene-Deleted Pseudorabies Virus Vaccines Mediated by CRISPR/Cas9 and Cre/Lox Systems

Viruses ◽

10.3390/v12040369 ◽

2020 ◽

Vol 12 (4) ◽

pp. 369

Author(s):

Jianglong Li ◽

Kui Fang ◽

Zhenxiang Rong ◽

Xinxin Li ◽

Xujiao Ren ◽

...

Keyword(s):

Immune Response ◽

Cd8 T Cells ◽

Humoral Immune Response ◽

Pseudorabies Virus ◽

Vaccine Candidate ◽

Clinical Signs ◽

Humoral Immune ◽

Viral Shedding ◽

Ifn Γ ◽

Efficacious Vaccine

Pseudorabies (PR), caused by pseudorabies virus (PRV), is an acute and febrile infectious disease in swine. To eradicate PR, a more efficacious vaccine needs to be developed. Here, the gE/gI- and TK/gE/gI-gene-deleted recombinant PRV (rGXΔgE/gI and rGXΔTK/gE/gI) are constructed through CRISPR/Cas9 and Cre/Lox systems. We found that the rGXΔTK/gE/gI was safer than rGXΔgE/gI in mice. Additionally, the effects of rGXΔgE/gI and rGXΔTK/gE/gI were further evaluated in swine. The rGXΔgE/gI and rGXΔTK/gE/gI significantly increased numbers of IFN-γ-producing CD4+ and CD8+ T-cells in swine, whereas there was no difference between rGXΔgE/gI and rGXΔTK/gE/gI. Moreover, rGXΔgE/gI and rGXΔTK/gE/gI promoted a PRV-specific humoral immune response. The PRV-specific humoral immune response induced by rGXΔgE/gI was consistent with that caused by rGXΔTK/gE/gI. After the challenge, swine vaccinated with rGXΔgE/gI and rGXΔTK/gE/gI showed no clinical signs and viral shedding. However, histopathological detection revealed that rGXΔgE/gI, not rGXΔTK/gE/gI, caused pathological lesions in brain and lung tissues. In summary, these results demonstrate that the TK/gE/gI-gene-deleted recombinant PRV was safer compared with rGXΔgE/gI in swine. The data imply that the TK/gE/gI-gene-deleted recombinant PRV may be a more efficacious vaccine candidate for the prevention of PR.

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