The influences of chokeberry extract supplementation on redox status and body composition in handball players during competition phase

2020 ◽  
Author(s):  
Nikola Cikiriz ◽  
Isidora Milosavljevic ◽  
Biljana Jakovljevic ◽  
Stefani Bolevich ◽  
Jovana Jeremic ◽  
...  
Keyword(s):  
Body Composition ◽  
High Density Lipoprotein ◽  
Blood Cells ◽  
Biochemical Parameters ◽  
Density Lipoprotein ◽  
Redox Status ◽  
The Other ◽  
Beneficial Effects ◽  
Wide Range ◽  
Biochemical Testing

The aim of our study was to investigate the influence of twelve-week consumption of chokeberry extract on redox status, body composition, lipid profile and biochemical parameters in active handball players. The study included 16 handball players, aged 16-24 years (20.26±2.86 years). Every morning before training players received 30mL of liquid chokeberry extract for 12 weeks, during regular competition season. The research consisted of morphofunctional and biochemical testing, which was performed at three points (at the beginning of the study, 6 and 12 weeks after extract consumption). After the chokeberry extract treatment, we noticed significant changes in three main aspects. The 12-week supplementation with chokeberry extract decreased the levels of pro-oxidants (TBARS and nitrites) and increased catalase activity. Analyzing the dynamic of body composition showed a decrease in body fat (9.4±0.5 vs. 7.3±0.6kg), as well as its percent in a body (11.4±0.4 vs. 8.8±0.4%). On the other hand, the analysis showed an increase of high-density lipoprotein (1.3±0.3 vs. 1.6±0.2mmol/L) and hemoglobin (144.4±11.7 vs. 151.7±9.9g/L) after 6-week treatment. At the same time, a decrease in leukocytes (7.2±2.8 vs. 6.5±1.2 109/L) and an increase in red blood cells count (4.9±0.4 vs. 5.5±0.5 109/L) were noticed. Overall, these results emphatically show that the use of chokeberry extract dietary supplement induced a wide range of beneficial effects in the examined group of athletes.

MiR-145-5p modulates lipid metabolism and M2 macrophage polarization by targeting PAK7 and regulating β-catenin signaling in hyperlipidemia

2021 ◽  
pp. 1-7
Author(s):  
Huijun Chen ◽  
Jing Gao ◽  
Qian Xu ◽  
Dongmei Wan ◽  
Wenji Zhai ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
High Density Lipoprotein ◽  
Macrophage Polarization ◽  
Density Lipoprotein ◽  
High Density ◽  
M2 Macrophage ◽  
Cardiac Tissues ◽  
Potential Biomarker ◽  
Wide Range ◽  
M2 Macrophage Polarization

The present study aims to explore the role of microRNA 145-5p (miR-145-5p) in hyperlipidemia. Using bioinformatics tools and a wide range of function and mechanism assays, we attempted to understand the specific function and potential mechanism of miR-145-5p in hyperlipidemia. A cholesterol-enriched diet induced an increase of serum cholesterol and triacylglycerol but a decrease of serum high-density lipoprotein. MiR-145-5p level was decreased in hyperlipidemia rat models. MiR-145-5p regulated lipid metabolism by antagonizing the alteration of high-density lipoprotein, cholesterol, and triacylglycerol in serum mediated by a cholesterol-enriched diet. In mechanism, miR-145-5p directly bound with p21 protein (RAC1)-activated kinase 7 (PAK7) and negatively regulated mRNA and protein levels of PAK7 in THP-1 cells. Furthermore, miR-145-5p level was negatively associated with PAK7 level in rat cardiac tissues. Finally, overexpression of PAK7 reversed the effects of miR-145-5p on β-catenin activation and M2 macrophages polarization in THP-1 cells. In conclusion, MiR-145-5p modulated lipid metabolism and M2 macrophage polarization by targeting PAK7 and regulating β-catenin signaling in hyperlipidemia, which may provide a potential biomarker for the treatment of hyperlipidemia-induced cardiovascular diseases.

Beneficial effects of an intradialytic cycling training program in patients with end-stage kidney disease

2015 ◽  
Vol 40 (6) ◽  
pp. 550-556 ◽  
Author(s):  
Carole Groussard ◽  
Myriam Rouchon-Isnard ◽  
Céline Coutard ◽  
Fanny Romain ◽  
Ludivine Malardé ◽  
...  
Keyword(s):  
Body Composition ◽  
Kidney Disease ◽  
Physical Fitness ◽  
Lipid Profile ◽  
Antioxidant Status ◽  
Density Lipoprotein ◽  
Control Group ◽  
Beneficial Effects ◽  
Cycling Training ◽  
Training Protocol

In chronic kidney disease (CKD), oxidative stress (OS) plays a central role in the development of cardiovascular diseases. This pilot program aimed to determine whether an intradialytic aerobic cycling training protocol, by increasing physical fitness, could reduce OS and improve other CKD-related disorders such as altered body composition and lipid profile. Eighteen hemodialysis patients were randomly assigned to either an intradialytic training (cycling: 30 min, 55%–60% peak power, 3 days/week) group (EX; n = 8) or a control group (CON; n = 10) for 3 months. Body composition (from dual-energy X-ray absorptiometry), physical fitness (peak oxygen uptake and the 6-minute walk test (6MWT)), lipid profile (triglycerides (TG), total cholesterol, high-density lipoprotein, and low-density lipoprotein (LDL)), and pro/antioxidant status (15-F2α-isoprostanes (F2-IsoP) and oxidized LDL in plasma; superoxide dismutase, glutathione peroxidase, and reduced/oxidized glutathione in erythrocytes) were determined at baseline and 3 months later. The intradialytic training protocol did not modify body composition but had significant effects on physical fitness, lipid profile, and pro/antioxidant status. Indeed, at 3 months: (i) performance on the 6MWT was increased in EX (+23.4%, p < 0.001) but did not change in CON, (ii) plasma TG were reduced in EX (–23%, p < 0.03) but were not modified in CON, and (iii) plasma F2-IsoP concentrations were lower in EX than in CON (–35.7%, p = 0.02). In conclusion, our results show that 30 min of intradialytic training, 3 times per week for 3 months, are enough to exert beneficial effects on the most sensitive and reliable marker of lipid peroxidation (IsoP) while improving CKD-associated disorders (lipid profile and physical fitness). Intradialytic aerobic cycling training represents a useful and easy strategy to reduce CKD-associated disorders. These results need to be confirmed with a larger randomized study.

scholarly journals Three routine methods for measuring high-density lipoprotein cholesterol compared with the Reference Method

1996 ◽  
Vol 42 (5) ◽  
pp. 738-743 ◽  
Author(s):  
N Harris ◽  
V Galpchian ◽  
N Rifai
Keyword(s):  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Dextran Sulfate ◽  
Direct Method ◽  
Density Lipoprotein ◽  
Reference Method ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Direct Assay ◽  
Wide Range

Abstract We compared the performance of three methods for quantifying high-density lipoprotein cholesterol (HDL-C) with the Reference Method for HDL-C, using samples with a wide range of triglyceride (TG) concentrations (290-18000 mg/L). All three comparison assays-- utilizing a magnetic dextran sulfate precipitating reagent, a direct method, and a standard MgCl2-dextran sulfate reagent--were precise, with a run-to-run CV of less than or equal to 4.1%. However, the systematic error of these assays exceeded the National Cholesterol Education Program (NCEP) performance goal of less than or equal to 10% in half of the concentration ranges tested. Nevertheless, the total error of the assays generally meets the current 22% limit set by the NCEP. Although both the magnetic dextran sulfate precipitation reagent and the direct assay can be performed more rapidly than the MgCl2-dextran sulfate assay, the direct assay involves no sample preparation and requires only 4 microL of sample excluding the dead space. Although precipitation is frequently inadequate with the MgCl2-dextran sulfate reagent at TG concentrations &gt;6000 mg/L, both the magnetic and the direct reagent show no interference from high TG concentrations as great as 18 000 mg/L.

scholarly journals 3-D Structure of Serum Paraoxonase 1 Sheds Light on Its Activity, Stability, Solubility and Crystallizability

2007 ◽  
Vol 58 (3) ◽  
pp. 347-353 ◽  
Author(s):  
Michal Harel ◽  
Boris Brumshtein ◽  
Ran Meged ◽  
Hay Dvir ◽  
Raimond Ravelli ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Catalytic Activity ◽  
High Density Lipoprotein ◽  
Active Site ◽  
Density Lipoprotein ◽  
Paraoxonase 1 ◽  
Hydrolytic Activities ◽  
Wide Range ◽  
The Stability ◽  
Serum Paraoxonase

3-D Structure of Serum Paraoxonase 1 Sheds Light on Its Activity, Stability, Solubility and CrystallizabilitySerum paraoxonases (PONs) exhibit a wide range of physiologically important hydrolytic activities, including drug metabolism and detoxification of nerve gases. PON1 and PON3 reside on high-density lipoprotein (HDL) (the "good cholesterol"), and are involved in the alleviation of atherosclerosis. Members of the PON family have been identified not only in mammals and other vertebrates, but also in invertebrates. We earlier described the first crystal structure of a PON family member, a directly-evolved variant of PON1, at 2.2 Å resolution. PON1 is a 6-bladed beta-propeller with a unique active-site lid which is also involved in binding to HDL. The 3-D structure, taken together with directed evolution studies, permitted analysis of mutations which enhanced the stability, solubility and crystallizability of this PON1 variant. The structure permits a detailed description of PON1's active site and suggests possible mechanisms for its catalytic activity on certain substrates.

scholarly journals Triglyceride/High-Density Lipoprotein Cholesterol Ratio is associated with the mortality of COVID-19: An Observational Study

2021 ◽  
Author(s):  
Fei Peng ◽  
Shangjie Wu ◽  
Si Lei ◽  
Quan Zhang ◽  
Yanjun Zhong
Keyword(s):  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Density Lipoprotein ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Operating Characteristics ◽  
Cholesterol Ratio

Abstract (1) Background: Triglyceride to high density lipoprotein cholesterol (TG/HDL-c) ratio is crucial when researching metabolic and vascular diseases, and its involvement in COVID-19 was sparsely elaborated on. The purpose of the study was to explore if there were any associations between the TG/HDL-c ratio and COVID-19 prognosis; (2) Methods: A total of 262 COVID-19 patients were retrospectively investigated. The clinical features and baseline hematological parameters were recorded and analyzed; (3) Results: Compared with the survivors, the non-survivors of COVID-19 had significantly higher levels of white blood cells (4.7 vs. 13.0 ×109/L; P < 0.001), neutrophils (3.0 vs. 11.6×109/L; P < 0.001), C-reactive proteins (15.7 vs. 76.7 mg/L; P < 0.001) and TG/HDL-c ratio (1.4 vs. 2.5; P = 0.001). The receiver operating characteristics curve [area under the curve, 0.731; 95% confidence interval, 0.609–0.853; P = 0.001] suggested that the TG/HDL-c ratio could predict the mortality of COVID-19. Moreover, the TG/HDL-c ratio was positively correlated with white blood cells (r = 0.255, P < 0.001), neutrophils (r = 0.243, P < 0.001) and C-reactive proteins (r = 0.170, P < 0.006); (4) Conclusions: Our study demonstrated that TG/HDL-c ratio may potentially be a predictive marker for mortality in COVID-19 patients.

249 BIOPHYSICAL AND BIOCHEMICAL CHARACTERISTICS OF ALPACA SEMEN AFTER COLLECTION BY ELECTROEJACULATION

2013 ◽  
Vol 25 (1) ◽  
pp. 272 ◽  
Author(s):  
J. Rodriguez ◽  
W. Huanca ◽  
M. Ramos ◽  
M. Vasquez ◽  
J. Espinoza
Keyword(s):  
Alkaline Phosphatase ◽  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Density Lipoprotein ◽  
Alanine Transaminase ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Biochemical Characteristics

The collection of alpaca semen presents difficulties because of the characteristics of mounts and copulation time in addition to the high viscosity of semen. The biophysical and biochemical characteristics of alpaca semen were investigated. Semen samples from four adult males from 6 to 7 years of age were collected using an electroejaculation technique. The biophysical parameters measured in seminal plasma included volume, color, sperm concentration, motility, and viscosity, and the biochemical parameters included pH and glucose, calcium, triglyceride, cholesterol, high-density lipoprotein cholesterol, total protein, albumin, alanine transaminase, alkaline phosphatase, and γ-glutamyltranspeptidase concentrations. The mean time for semen collection was 10.3 ± 1.5 min; the volume varied from 1.2 to 3.8 and the colour from clear to milky, and pH was 7.4 ± 0.03. Mean concentration of spermatozoa was 69.3 ± 193 million mL–1, and motility was 52.4 ± 9.7%. Biochemical parameters were glucose (mg dL–1) 8.22 ± 0.77; cholesterol (mg dL–1) 79.78 ± 5.64; triglycerides (mg dL–1) 44.12 ± 7.38; high-density lipoprotein cholesterol (mg dL–1) 4.73 ± 0.30; total protein (g dL–1) 2.36 ± 0.15; albumin (g dL–1) 0.97 ± 0.33; calcium (mg dL–1) 11.77 ± 1.74; alanine transaminase (U L–1) 17.92 ± 9.09; alkaline phosphatase (U L–1) 288.76 ± 279.59; and γ-glutamyltranspeptidase (U L–1) 89.62 ± 39.09. These results provide information on the biophysical and biochemical characteristics of alpaca semen collected by electroejaculation, with variable results in the enzymatic components.

scholarly journals CORRELATION OF NON-HIGH-DENSITY LIPOPROTEIN CHOLESTEROL WITH LIPOPROTEIN(A) IN DIABETIC PATIENTS

2018 ◽  
Vol 11 (9) ◽  
pp. 499
Author(s):  
Vasanthan M
Keyword(s):  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Density Lipoprotein ◽  
High Density ◽  
The Other ◽  
Lipoprotein Cholesterol ◽  
Diabetic Patients ◽  
Healthy Controls ◽  
Lipoprotein A ◽  
Artery Disease

Objective: The objective of this study is to estimate the level of lipoprotein(a) and lipid profile, especially non-high-density lipoprotein cholesterol (non-HDL-C) in diabetic patients, and to correlate the same with healthy controls (non-diabetics).Methods: A total of 30 non-diabetic subjects and 30 diabetic patients were included in the study. Lipoprotein(a) (Lp[a]) was estimated by immunoturbidimetry and the other parameters by their respective methods in biochemistry auto-analyzer.Result: From the study, it was found that both Lp(a) and non-HDLC were increased in diabetics when compared to the non-diabetic controls. It was also evident that there is a positive correlation of Lp(a) levels with non-HDL C.Conclusion: Lp(a) and non-HDL C are responsible for atherogenic events in uncontrolled diabetic patients, leading to risks such as coronary artery disease.

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