Changes in Blood Chemistry of Juvenile Steelhead Trout, Salmo gairdneri, Following Sublethal Exposure to Nitrogen Supersaturation

1974 ◽  
Vol 31 (12) ◽  
pp. 1953-1957 ◽  
Author(s):  
T. W. Newcomb
Keyword(s):  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Lactate Dehydrogenase ◽  
Total Protein ◽  
Total Bilirubin ◽  
Blood Chemistry ◽  
Salmo Gairdneri ◽  
Steelhead Trout ◽  
Serum Samples ◽  
Serum Glutamic Oxalacetic Transaminase

Groups of juvenile steelhead trout (Salmo gairdneri) were exposed for 35 days to various (103, 105, 110, and 116%) sublethal nitrogen plus argon saturations. Pooled serum samples were analyzed for Ca, Na, PO4, K, Cl, albumin, total protein, cholesterol, alkaline phosphatase, glucose, urea, uric acid, total bilirubin, lactate dehydrogenase, and serum glutamic oxalacetic transaminase. An increase in serum potassium and phosphate, and a decline in serum albumin, calcium, cholesterol, total protein and alkaline phosphatase were noted in steelhead exposed to 116% nitrogen (N2 + Ar) saturation (total atmospheric gas saturation 110%). No major changes in blood chemistry were observed at nitrogen saturations of 110% or less.

Correlation of Selected Serum Constituents: 1. Inter-Individual Variation and Analytical Error

1975 ◽  
Vol 21 (11) ◽  
pp. 1592-1600 ◽  
Author(s):  
Per Winkel ◽  
Bernard E Statland ◽  
Henning Bokelund ◽  
Eugene A Johnson
Keyword(s):  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Lactate Dehydrogenase ◽  
Aspartate Aminotransferase ◽  
Correlation Coefficients ◽  
Serum Samples ◽  
Significant Component ◽  
Healthy Men ◽  
Analytical Error ◽  
Analytical Errors

Abstract The intra-subject correlations of three clinically meaningful combinations of serum constituents—(a) potassium, calcium, and albumin; (b) urea, creatinine, and uric acid; and (c) aspartate aminotransferase, lactate dehydrogenase, and alkaline phosphatase—were studied in 11 healthy men. Duplicate serum samples were obtained at 800 h, 1100 h, and 1400 h on five different days. All assays were performed on the AutoChemist Multichannel Analyzer. Correlation coefficients differed significantly among the subjects for the following six pairs of serum constituents: urea and creatinine, urea and uric acid, creatinine and uric acid, aspartate aminotransferase and lactate dehydrogenase, aspartate aminotransferase and alkaline phosphatase, and lactate dehydrogenase and alkaline phosphatase. Nonbiological positive correlation between analytical errors (i.e., errors of two different assays performed on the same specimen) was demonstrated for two of the pairs: potassium and calcium, and aspartate aminotransferase and lactate dehydrogenase. The error correlations of these two pairs of constituents comprised a significant component of the observed intra-subject correlations. Probable reasons for these analytical error correlations are discussed

scholarly journals Blood plasma chemistry in White Sea bearded seals across different age groups

2019 ◽  
Vol 19 (4) ◽  
pp. 159-165
Author(s):  
IA Erokhina ◽  
NN Kavtsevich
Keyword(s):  
Alkaline Phosphatase ◽  
Creatine Kinase ◽  
Uric Acid ◽  
Lactate Dehydrogenase ◽  
White Sea ◽  
Blood Chemistry ◽  
Total Lipids ◽  
Bearded Seal ◽  
Γ Globulins ◽  
Glutamyl Transferase

Blood chemistry values are reported for the bearded seal species (Erignathus barbatus barbatus Erxleben, 1777) from the White Sea. 27 blood plasma indices are used to describe the state of the metabolism of proteins, carbohydrates, lipids, minerals (total protein, albumin, α-, β-, γ-globulins, urea, creatinine, uric acid, glucose, lactic acid, total lipids, triglycerides, cholesterol, calcium, phosphorus, sodium, potassium, magnesium, iron, copper, chlorides, aspartate aminotransferase, alanine aminotransferase, γ-glutamyl transferase, creatine kinase, alkaline phosphatase, lactate dehydrogenase) in 3-, 5- and 10-year-old seals. The values of the studied parameters are similar to published data for bearded seal populations in other Arctic regions, as well as being in close agreement with indicators of other pinniped species of the same age. The plasma content of total protein, albumin, α-, β-, γ-globulins, urea, uric acid, total lipids, calcium, magnesium, sodium, and chlorides is invariable in animals of the studied ages. In common with other pinnipeds and land-based mammals, age-related changes in metabolic rates in the studied animal groups are most pronounced in the activity level of key metabolism enzymes. While aspartate aminotransferase, alanine aminotransferase and γ-glutamyl transferase activity is higher in adult seals than in immature animals, alkaline phosphatase, creatine kinase and lactate dehydrogenase activity is lower. Changes in biochemical parameters of bearded seal blood at the ages of 3, 5 and 10 years indicate a catabolic orientation in metabolism at all studied development stages. Although more research is needed to investigate the biological significance of a number of blood indices, the data presented in this study provide initial baseline blood chemistry parameters for use in assessing the condition of individual seals, as well as informing monitoring and management efforts for wild seal populations.

Serum-constituents analyses: effect of duration and temperature of storage of clotted blood.

1981 ◽  
Vol 27 (1) ◽  
pp. 35-38 ◽  
Author(s):  
T Ono ◽  
K Kitaguchi ◽  
M Takehara ◽  
M Shiiba ◽  
K Hayami
Keyword(s):  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Lactate Dehydrogenase ◽  
Total Protein ◽  
Zinc Sulfate ◽  
Leucine Aminopeptidase ◽  
Inorganic Phosphorus ◽  
Serum Urea ◽  
Gamma Glutamyltransferase ◽  
Thymol Turbidity

Abstract We studied the effects on 25 analytes of duration of contact of serum with non-anticoagulated blood and of temperature. Serum was separated after blood was allowed to stand, for 0, 2, 4, 6, 8, 24, or 48 h at 4, 23, or 30 degrees C. Results obtained for bilirubin, albumin, zinc sulfate turbidity, thymol turbidity, cholinesterase (EC 3.1.1.8), alkaline phosphatase (EC 3.1.3.1), leucine aminopeptidase (EC 3.4.11.1), amylase (EC 3.2.1.2), total cholesterol, triglycerides, beta-lipoprotein, serum urea nitrogen, creatinine, uric acid, and gamma-glutamyltransferase (EC 2.3.2.2) were not influenced by storage at 4, 24, or 30 degrees C for as long as 48 h. Negligible differences were seen for potassium in sera in contact with cells as long as 24 h at 23 degrees C and for inorganic phosphorus after 48 h at 4 degrees C. However, at 4 degrees C we noted an increase at 8 h, a slight decrease at 30 degrees C. Statistically significant changes were seen for total protein and calcium after 48 h at 30 degrees C; for aspartate aminotransferase (EC 2.6.1.1), and alanine aminotransferase (EC 2.6.1.2), between 8 and 24 h at 23 degrees C and as soon as 6 h at 30 degrees C; for lactate dehydrogenase (EC 1.1.1.27) after 8 h at 30 degrees C and between 8 and 24 h at 23 degrees C; for glucose at 24, 4, or 2 h of storage at 4, 23, or 30 degrees C, respectively; for inorganic phosphorus after 48 h at 23 degrees C or 8 h at 30 degrees C; for potassium after 4 h at 4 degrees C or 24 h at 30 degrees C; and for sodium after 48 h at 4 degrees C or 6 h at 23 or 30 degrees C.

scholarly journals Prognosis of chronic lymphocytic leukemia: a multivariate regression analysis of 325 untreated patients

Blood ◽  
1987 ◽  
Vol 69 (3) ◽  
pp. 929-936 ◽  
Author(s):  
JS Lee ◽  
DO Dixon ◽  
HM Kantarjian ◽  
MJ Keating ◽  
M Talpaz
Keyword(s):  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Regression Analysis ◽  
Lactate Dehydrogenase ◽  
Chronic Lymphocytic Leukemia ◽  
Regression Model ◽  
Multivariate Regression ◽  
Risk Groups ◽  
Multivariate Regression Analysis ◽  
Lymphocytic Leukemia

Three hundred twenty-five previously untreated patients with chronic lymphocytic leukemia were analyzed to identify significant prognostic factors for survival. Univariate analysis identified the following characteristics associated with survival: (1) clinical characteristics: age, race, sex, performance status, lymphadenopathy, and hepatosplenomegaly; (2) hematologic parameters: WBC count, absolute lymphocyte and granulocyte counts, hemoglobin level, and platelet count; and (3) biochemical parameters: serum albumin, calcium, uric acid, lactate dehydrogenase, alkaline phosphatase, BUN, and creatinine. Multivariate regression analysis in a randomly selected training subset of 217 patients demonstrated that the combination of uric acid, alkaline phosphatase, lactate dehydrogenase, external lymphadenopathy, and age had the strongest predictive relation to survival time. The resulting model was validated in the remaining independent subset of 108 patients and led to classification of patients into low, intermediate, and high-risk groups with five-year survival rates of 75%, 59%, and 14%, respectively, and with distinctively different annual mortality rates (P less than .01). Both the regression model and Rai staging were highly effective in identifying risk groups among the entire patient population (P less than 0.001). Overall the regression model was superior to Rai staging in defining prognostic risk groups. In addition, it was able to separate patients into significantly different risk categories within each Rai stage, thus improving on the prognostic prediction of individual patients with chronic lymphocytic leukemia.

An Evaluation of the Greiner Selective Analyzer and its Role in the Clinical Laboratory

1975 ◽  
Vol 12 (1-6) ◽  
pp. 182-191 ◽  
Author(s):  
Andrew G. Skinner ◽  
Peter Wilding
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Creatinine ◽  
Total Protein ◽  
Total Bilirubin ◽  
Clinical Laboratory ◽  
Comparison Of Results ◽  
Operation Temperature ◽  
Accuracy And Precision ◽  
Analytical Range

The Greiner Selective Analyzer (GSA II) was evaluated over a period of six months. The evaluation assessed the reliability, accuracy, and precision of the analyser for six determinations. The methods evaluated were for glucose, urea, creatinine, total protein, total bilirubin, and alkaline phosphatase. Comparison of results was also made with those obtained for the same specimens using the Technicon SMA 12/60 Analyzer. Correlation and comparison of results indicate that the Greiner Selective Analyzer performed better for three of the methods but worse for serum creatinine determination. The role of the analyser as a routine tool in the clinical laboratory was also evaluated during analyses of approximately 900 patient specimens. Other features evaluated were analytical range of the six methods under study, the economics of operation, temperature control, and electrical and mechanical safety.

scholarly journals Prognosis of chronic lymphocytic leukemia: a multivariate regression analysis of 325 untreated patients

Blood ◽  
1987 ◽  
Vol 69 (3) ◽  
pp. 929-936 ◽  
Author(s):  
JS Lee ◽  
DO Dixon ◽  
HM Kantarjian ◽  
MJ Keating ◽  
M Talpaz
Keyword(s):  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Regression Analysis ◽  
Lactate Dehydrogenase ◽  
Chronic Lymphocytic Leukemia ◽  
Regression Model ◽  
Multivariate Regression ◽  
Risk Groups ◽  
Multivariate Regression Analysis ◽  
Lymphocytic Leukemia

Abstract Three hundred twenty-five previously untreated patients with chronic lymphocytic leukemia were analyzed to identify significant prognostic factors for survival. Univariate analysis identified the following characteristics associated with survival: (1) clinical characteristics: age, race, sex, performance status, lymphadenopathy, and hepatosplenomegaly; (2) hematologic parameters: WBC count, absolute lymphocyte and granulocyte counts, hemoglobin level, and platelet count; and (3) biochemical parameters: serum albumin, calcium, uric acid, lactate dehydrogenase, alkaline phosphatase, BUN, and creatinine. Multivariate regression analysis in a randomly selected training subset of 217 patients demonstrated that the combination of uric acid, alkaline phosphatase, lactate dehydrogenase, external lymphadenopathy, and age had the strongest predictive relation to survival time. The resulting model was validated in the remaining independent subset of 108 patients and led to classification of patients into low, intermediate, and high-risk groups with five-year survival rates of 75%, 59%, and 14%, respectively, and with distinctively different annual mortality rates (P less than .01). Both the regression model and Rai staging were highly effective in identifying risk groups among the entire patient population (P less than 0.001). Overall the regression model was superior to Rai staging in defining prognostic risk groups. In addition, it was able to separate patients into significantly different risk categories within each Rai stage, thus improving on the prognostic prediction of individual patients with chronic lymphocytic leukemia.

scholarly journals Effect of decocted turmeric on performance, hematological parameters and carcass traits of broiler chickens

2017 ◽  
Vol 42 (4) ◽  
pp. 263
Author(s):  
I. Isroli ◽  
T. Yudiarti ◽  
E. Widiastuti ◽  
S. Sugiharto
Keyword(s):  
Uric Acid ◽  
Stress Responses ◽  
Total Protein ◽  
Broiler Chickens ◽  
Hematological Parameters ◽  
Carcass Traits ◽  
Blood Chemistry ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Breast Meat

This study aimed to investigate the effect of decocted turmeric on performance, haematological parameters and carcass traits of broilers. Broiler chicks at 11 days of age were allotted to five groups, including T0 (birds drinking 100% water), T1 (25% decocted turmeric + 75% water), T2 (50% decocted turmeric + 50% water), T3 (75% decocted turmeric + 25% water) and T4 (100% decocted turmeric). The study was arranged based on a completely randomized design (CRD), parameters investigated was performance and blood chemistry (leukocyte, total protein, albumin, globulin, uric acid, glucose). The data were subjected to analysis of variance at 5% probability. Decocted turmeric did not affect weight gain, feed and water consumption and feed conversion ratio of broilers. Lymphocytes proportion was higher (P<0.05) in T1, T2 and T4 than in T0 and T3 birds. The heterophils to lymphocytes ratio was lower (P<0.05) in birds receiving decocted turmeric than control. Globulin concentration and albumin to globulin ratio were higher and lower (P<0.05), respectively, in serum of T2, T3 and T4 than T0 and T1 birds. The proportion of heterophils and concentration of serum total protein, albumin, uric acid and glucose were not different among treatments. The weight of breast meat was higher and lower (P<0.05) in T3 and T4 birds, respectively, compared to the control. In conclusion, providing decocted turmeric through drinking water helped to improve stress responses and increased the weight of breast meat of broiler chickens. 

scholarly journals Comparison of a full-spectrum multi-analyte clinical analyser with six reference instruments using canine and feline blood samples

2017 ◽  
Vol 62 (No. 6) ◽  
pp. 342-350
Author(s):  
CS Lin ◽  
GH Chiang ◽  
CH Liu ◽  
HC Tsai ◽  
CC Yang ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Bile Acid ◽  
Blood Urea Nitrogen ◽  
Total Protein ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Total Bilirubin ◽  
Total Bile Acid ◽  
Analytical Performance ◽  
Blood Samples

In this study, we report the characterisation of a novel centrifugation and spectrum-integrated veterinary clinical analyser, the AmiShield<sup>TM</sup>, which has been developed for the multiplex measurement of biochemical, electrolyte and immunoassay parameters in a point-of-care testing environment. The aims of this study were to evaluate the analytical performance of the AmiShield<sup>TM</sup> and to compare it with six reference instruments using clinical blood samples. Two hundred and four canine and 120 feline blood samples collected from veterinary teaching hospitals were analysed in parallel using the AmiShield and appropriate reference instruments. All results were evaluated separately for canine and feline specimens. The instrument’s analytical performance was evaluated initially for short- and long-term precision, bias, and observed total error using quality control material. This was followed by comparison of clinical specimens on the AmiShield analyser in parallel with the Vitros and Hitachi for biochemical parameters, VetScan and SNAPshot for total bile acids, and VetLyte and Biolyte for electrolytes. Overall, the AmiShield analyser’s performance met the standards of the American Society for Veterinary Clinical Pathology for total allowable error for most analytes, and can be considered suitable for use in veterinary clinical practices. Using canine samples, excellent correlation coefficients (r ≧ 0.92) were identified for 14 analytes of various categories including glucose, total protein, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total bilirubin, amylase, blood urea nitrogen, creatinine, phosphorus, Na<sup>+</sup>, K<sup>+</sup>, Cl<sup>–</sup> and total bile acid, while good correlations (0.91 ≧ r ≧ 0.80) were recorded for albumin (r = 0.91). Bland-Altman difference plots also showed agreement (greater than 95% within Limits of Agreement) for glucose, total protein, albumin, alanine aminotransferase, alkaline phosphatase, total bilirubin, amylase, blood urea nitrogen, creatinine, Na<sup>+</sup>, K<sup>+</sup>, Cl<sup>–</sup> and total bile acid between AmiShield and the reference instruments. However, aspartate aminotransferase and phosphorus exhibited higher outliers, implying potential problems associated with matrix interferences such as lipemic samples, which warrant further study. This study demonstrates that the AmiShield compares favourably with standard reference instruments, and the new device generated data of high quality for most analytes in clinical canine and feline samples. The capability of reliably measuring multi-category analytes in one device using minute amounts (170 μl) of whole blood and short turn-around times (&lt; 15 min) underlines the high potential of the device as a good alternative in-house diagnostic application.

Swimming Stamina Differences Between Genotypically Distinct Forms of Rainbow (Salmo gairdneri) and Steelhead Trout

1977 ◽  
Vol 34 (7) ◽  
pp. 996-1003 ◽  
Author(s):  
H. Tsuyuki ◽  
S. N. Williscroft
Keyword(s):  
Rainbow Trout ◽  
Lactate Dehydrogenase ◽  
Key Words ◽  
Low Frequency ◽  
Water Velocity ◽  
Salmo Gairdneri ◽  
Steelhead Trout ◽  
Very Low Frequency ◽  
Time Required

Rainbow trout (Salmo gairdneri) homozygous for liver lactate dehydrogenase alleles ldhHαA and ldhHαB were artificially propagated and their swimming stamina compared. The time required to fatigue 50% of the HαAHαA phenotypes in fixed water velocity tests was 2.3 times greater on the average than that of HαAHαB phenotypes.Likewise, LDH phenotypes HαAHαA, HαAHαB, and HαAHαB of steelhead trout from the Thompson River were artifically propagated and their swimming stamina compared. In contrast to the rainbow trout, significant differences in stamina among the three phenotypes of steelhead were not evident in the stocks from this river nor between phenotypes HαAHαB and HαBHαB from another stream, the Vedder River, which has a very low frequency of the ldhHαA allele. The stamina of young steelhead from the Thompson River was, however, 3.8 times greater than that of those from the Vedder River. Key words: stamina, LDH, rainbow trout, steelhead, phenotype, swimming, fatigue

scholarly journals Effect of Dietary Extruded Linseed and Walnut Meal Mixture (8:1) on Performance and Plasma Protein Profile in Weaned Piglets

2018 ◽  
Vol 75 (2) ◽  
pp. 121 ◽  
Author(s):  
Anca GHEORGHE ◽  
Mihaela HĂBEANU ◽  
Nicoleta Aurelia LEFTER ◽  
Daniela Mihaela GRIGORE
Keyword(s):  
Uric Acid ◽  
Plasma Protein ◽  
Soybean Meal ◽  
Total Protein ◽  
Total Bilirubin ◽  
Protein Profile ◽  
Weaned Piglets ◽  
Urea Nitrogen ◽  
Blood Samples ◽  
Dietary Nitrogen

The effects of dietary extruded linseed (ELS):walnut meal (WM) mixture (8:1) on performance and plasma protein profile in weaned piglets was evaluated for 21 d. Topigs piglets (n=40; BW=8.02±0.82 kg), age 30±3 days, were allotted into 2 groups and fed 2 diets: control [C, based on corn-triticale-soybean meal (SBM)] and experimental (ELS:WM, where the ELS:WM mixture (8:1) partially replace SBM). Blood samples were collected at 7d and 21d after weaning. The plasma protein profile (total protein, total bilirubin, albumin, creatinine, uric acid, urea nitrogen-BUN) were determined by a chemistry analyser. Dietary ELS:WM mixture improve the BW (P=0.047) and ADG (P=0.036) of piglets at 21d after weaning vs C, whereas ADFI and F:G ratio were increase (P>0.05). The plasma protein profile of piglets fed dietary mixture was not affected at 7d and 21d after weaning, except plasma BUN concentration that was decrease (P=0.027) at 21d after weaning. Lower BUN concentration indicated higher availability of dietary nitrogen reflected in a higher deposition of protein. We concluded that dietary ELS:WM mixture improve piglets performance and positively afect plasma protein profile, especially BUN in weaning period.

Sign in / Sign up

Export Citation Format

Share Document