Genetic diversity of the Bambara groundnut (Vigna subterranea (L.) Verdc.) as assessed by SSR markers

Genome ◽  
2011 ◽  
Vol 54 (11) ◽  
pp. 898-910 ◽  
Author(s):  
P. Somta ◽  
S. Chankaew ◽  
O. Rungnoi ◽  
P. Srinives
Keyword(s):  
Ssr Markers ◽  
Gene Diversity ◽  
Bambara Groundnut ◽  
West African ◽  
Azuki Bean ◽  
Vigna Subterranea ◽  
East African ◽  
The West ◽  
Legume Crop ◽  
Population Structure Analysis

Bambara groundnut ( Vigna subterranea (L.) Verdc.) is an important African legume crop. In this study, a collection consisting of 240 accessions was analyzed using 22 simple sequence repeat (SSR) markers. In total, 166 alleles were detected, with a mean of 7.59 alleles per locus. Allelic and gene diversities were higher in the west African and Cameroon/Nigeria regions with 6.68 and 6.18 alleles per locus, and 0.601 and 0.571, respectively. The genetic distance showed high similarity between west African and Cameroon/Nigeria accessions. Principal coordinate analyses and neighbor-joining analysis consistently revealed that the majority of west African accessions were grouped with Cameroon/Nigeria accessions, but they were differentiated from east African, central African, and southeast Asian accessions. Population structure analysis showed that two subpopulations existed, and most of the east African accessions were restricted to one subpopulation with some Cameroon/Nigeria accessions, whereas most of the west African accessions were associated with most of the Cameroon/Nigeria accessions in the other subpopulation. Comparison with SSR analysis of other Vigna cultigens, i.e., cultivated azuki bean ( Vigna angularis ) and mungbean ( Vigna radiata ), reveals that the mean gene diversity of Bambara groundnut was lower than azuki bean but higher than mungbean.

scholarly journals Assessment of genetic diversity and structure of Bambara groundnut [Vigna subterranea (L.) verdc.] landraces in South Africa

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Adré Minnaar-Ontong ◽  
Abe S. Gerrano ◽  
Maryke T. Labuschagne
Keyword(s):  
South Africa ◽  
Genetic Diversity ◽  
Ssr Markers ◽  
Southern Africa ◽  
Crop Improvement ◽  
Bambara Groundnut ◽  
Vigna Subterranea ◽  
Legume Crop ◽  
Population Structure Analysis ◽  
Genetic Diversity And Structure

AbstractWith its drought tolerant and protein-rich properties, Bambara groundnut [Vigna subterranea (L.) Verdc.], an indigenous African legume crop can contribute immensely to food security. This miracle crop is used as food and for the enhancement of soil fertility in South Africa. Knowledge on the genetic diversity and structure among the Bambara groundnut landraces can pave the way for the effective use and cultivation of this crop in southern Africa, especially South Africa. The aim of this study was to assess the genetic diversity and structure among Bambara groundnut landraces collected across South Africa and compared to a limited number of accessions from southern Africa using SSR markers. Seventy-eight Bambara groundnut accessions were genotyped using 19 Bambara specific SSR markers. SSR loci explored in this study, were all polymorphic. A total of 127 alleles were detected with a mean of 6.7 alleles per locus. Allele diversity and frequency among genotypes varied from 0.21 to 0.85 with an average of 0.62 per locus. Genetic variation as described by the analysis of molecular variance indicated higher genetic diversity (92%) within landraces than between (8%) different landraces. Population structure analysis showed that three subpopulations existed, and most of the South African accessions were restricted to one subpopulation, indicating that Bambara landraces has the ability to form unique haplotypes in different environments. Information harnessed in this study is helpful for further use in breeding programs for crop improvement.

scholarly journals Keragaman Genetik Kacang Bogor (Vigna subterranea L. Verdc.) Berdasarkan Marka SSR (Simple Sequence Repeat)

2017 ◽  
Vol 44 (3) ◽  
pp. 279
Author(s):  
Zikril Illahi ◽  
Ni Made Armini Wiendi ◽  
Dan Sudarsono
Keyword(s):  
Simple Sequence Repeat ◽  
Genetic Distances ◽  
Bambara Groundnut ◽  
Vigna Subterranea ◽  
Sequence Repeat ◽  
Phylogenic Tree ◽  
Legume Crop ◽  
Population Structure Analysis ◽  
Simple Sequence ◽  
Underutilized Legume

<em></em><em>ABSTRACT<br /><br />Bambara groundnut (Vigna subterranea L. Verdc.) is an important underutilized legume crop in Indonesia. The aim of this research is to study genetic diversity of bambara groundnut from Sukabumi and Sumedang, West Java, Indonesia. This study used 107 bambara groundnut accessions, which consisted of 57 accessions from Sukabumi and 50 accessions from Sumedang. We use five simple sequence repeat (SSR) to analyze the accessions. Totally nine alleles were detected, with a mean of 1.8 alleles per locus. Allelic and gene diversities were higher in Sumedang (1.8 alleles per locus and 0.119) than in Sukabumi population (1.4 alleles per locus and 0.020), respectively. We constructed a phylogenic tree by Neighbor-Joining analysis based on genetic distances (DA) and showed the tree divided bambara groundnut accessions into two broad groups according to the origin of samples (Sukabumi and Sumedang). Results from the phylogenic tree are in line with those from the population structure analysis.<br /> <br />Keywords: allele diversity, bambara groundnut, genetic distance, under utilized legume<br /><br /></em>

Genetic variability of Indonesian landraces of Vigna subterranea: Morphological characteristics and molecular analysis using SSR markers

2020 ◽  
Vol 21 (9) ◽  
Author(s):  
MUHAMMAD FAUZAN FARID ALHAMDI ◽  
Asep Setiawan ◽  
Satriyas Ilyas ◽  
Wai Kuan Ho
Keyword(s):  
Genetic Variability ◽  
Ssr Markers ◽  
Molecular Analysis ◽  
Block Design ◽  
Leaf Shape ◽  
Morphological Characteristics ◽  
Bambara Groundnut ◽  
Vigna Subterranea ◽  
Alternative Source ◽  
Randomized Block Design

Abstract. Alhamdi MFF, Setiawan A, Ilyas S, Ho WK. 2020. Genetic variability of Indonesian landraces of Vigna subterranea: The morphological characteristics and molecular analysis using SSR markers. Biodiversitas 21: 3929-3937. Bambara groundnut (Vigna subterranea (L.) Verdc.) is a potential grain, which can be considered as an alternative source of protein and carbohydrate. Due to unavailability of commercial bambara groundut cultivar in Indonesia, the characterization of bambara groundnut landraces is an important step before developing cultivar with traits of interest. The objective of the research was to access genetic variability of Indonesian landraces of bambara groundnut with different seed coat colors based on morphological and molecular markers. The experiment was arranged as split-plot in a complete randomized block design with the main plot was cultivation methods and the sub plot was landraces. There were differences in leaf shape and pod shape among the landraces. There were two main clusters of Indonesian landraces of bambara groundnut with 88.28% similarity. The first cluster was Cream, Brown Sumedang, Black Sumedang and Black Tasikmalaya, and the second cluster was Black Sukabumi, Brown Gresik, Black Madura, and Black Gresik. The result based on SSR marker with capillary electrophoresis indicated Black Gresik and Black Madura landraces were different from other Indonesian landraces.  Cream Sumedang or Brown Sumedang from the first cluster and Black Gresik or Brown Gresik from the second cluster have the farthest distances for developing improved variety of bambara groundnut.

Novel Centromeric and Subtelomeric Repetitive DNA Sequences for Karyotyping the Bambara Groundnut (Vigna subterranea L. Verdc.)

2022 ◽  
pp. 1-9
Author(s):  
Ahmet L. Tek ◽  
Sevim D. Kara Öztürk ◽  
Hümeyra Yıldız ◽  
Didem Karalar
Keyword(s):  
Dna Sequences ◽  
Bambara Groundnut ◽  
Single Pair ◽  
Vigna Subterranea ◽  
Telomeric Dna ◽  
Legume Crop ◽  
Climate Resilience ◽  
Subtelomeric Repeat ◽  
Rna Genes ◽  
Potential Use

Bambara groundnut (<i>Vigna subterranea</i> L. Verdc.) is an un­derutilized minor legume crop with climate resilience and great potential use in world agriculture. This study aimed to cytogenetically characterize the genome and chromosome properties of Bambara groundnut. We cloned, sequenced, and mapped a 50-bp centromere-specific tandem repeat on all chromosomes. In addition, a 400-bp subtelomeric repeat was discovered and mapped on a single pair of chromosomes. A Bambara groundnut karyotype was constructed using these novel repeats along with ribosomal RNA genes (45S and 5S) and telomeric DNA sequences. This study provides the first analysis of the genome and chromosome properties of Bambara groundnut. We discuss our findings in relation to genetic improvement of Bambara groundnut and centromere evolution in legume species.

scholarly journals The distribution of advanced tongue root harmony and interior vowels in the Macro-Sudan Belt

2017 ◽  
Vol 2 ◽  
pp. 10 ◽  
Author(s):  
Nicholas Revett Rolle ◽  
Matthew Faytak ◽  
Florian Lionnet
Keyword(s):  
Central Africa ◽  
Strong Relationship ◽  
West African ◽  
East African ◽  
The West ◽  
Language Varieties ◽  
Phonological Features ◽  
Vowel Systems ◽  
Chi Squared ◽  
Heterogeneous Region

In this paper we investigate the distribution of vowel systems in the Macro-Sudan Belt, an area of Western and Central Africa proposed in recent areal work (Güldemann 2008, 2011; Clements & Rialland 2008). We report on a survey of 615 language varieties with entries coded for two phonological features: advanced tongue root (ATR) harmony and the presence of interior vowels (i.e. non-peripheral vowels, such as [ɨ ɯ ɜ ə ʌ …]). Our results show that the presence of ATR harmony in the Macro-Sudan Belt is limited to three separated zones: an Atlantic ATR Zone, a West African ATR Zone, and an East African ATR Zone, all geographically unconnected to one another. We additionally show that between the West and East African ATR Zones is a geographically extensive, genetically heterogeneous region of Central Africa where ATR harmony is systematically absent which we term the Central African ATR-less Zone. Our results also show a large region where phonemic and allophonic interior vowels are disproportionately prevalent, which we term the Central African Interior Vowel Zone. This zone noticeably overlaps with the Central African ATR-less Zone, suggesting that ATR and interiority have an antagonistic relationship. Chi-squared tests support the presence of a strong relationship between the two types of vowel contrasts.

INFLUENCE OF SOWING DATE AND ENVIRONMENTAL FACTORS ON THE DEVELOPMENT AND YIELD OF BAMBARA GROUNDNUT (VIGNA SUBTERRANEA) LANDRACES IN A SUB-TROPICAL REGION

2008 ◽  
Vol 44 (2) ◽  
pp. 167-183 ◽  
Author(s):  
A. SESAY ◽  
C. N. MAGAGULA ◽  
A. B. MANSUETUS
Keyword(s):  
Environmental Factors ◽  
Rural Areas ◽  
Field Experiments ◽  
Sowing Date ◽  
Bambara Groundnut ◽  
Effect Of Temperature ◽  
Grain Legume ◽  
Tropical Region ◽  
Vigna Subterranea ◽  
Legume Crop

SUMMARYBambara groundnut (Vigna subterranea) is a grain legume crop, which is increasingly popular as food in rural areas across the African continent. However, reliable information pertaining to management of the crop is limited. Field experiments were conducted in Swaziland in the 1998/99 and 1999/2000 cropping seasons to determine the influence of sowing date and environmental factors on the growth, development and yield of bambara groundnut. In the 1998/99 season, seeds of one local landrace were sown on six dates between mid-September 1998 and mid-February 1999. In 1999/2000, seeds of two local landraces were sown on six dates between mid-October 1999 and late January 2000. The highest pod yield and total dry matter production were achieved in November sowings, with maximum pod yields of 1.3 and 0.64 t ha−1 for the 1998/99 and 1999/2000 seasons, respectively. Earlier sowing and successive delays in sowing from November caused substantial yield declines of as much as 72–75%. Sowing date influenced yields of bambara groundnut through the effect of temperature and daylength on plant development. For the landraces used in the study, while the rate of progress from sowing to flowering was influenced by temperature, the rate of progress from flowering to podding was influenced largely by daylength. The practical implications of the results for increased bambara groundnut production are discussed.

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