Novel Centromeric and Subtelomeric Repetitive DNA Sequences for Karyotyping the Bambara Groundnut (Vigna subterranea L. Verdc.)

2022 ◽  
pp. 1-9
Author(s):  
Ahmet L. Tek ◽  
Sevim D. Kara Öztürk ◽  
Hümeyra Yıldız ◽  
Didem Karalar

Bambara groundnut (<i>Vigna subterranea</i> L. Verdc.) is an un­derutilized minor legume crop with climate resilience and great potential use in world agriculture. This study aimed to cytogenetically characterize the genome and chromosome properties of Bambara groundnut. We cloned, sequenced, and mapped a 50-bp centromere-specific tandem repeat on all chromosomes. In addition, a 400-bp subtelomeric repeat was discovered and mapped on a single pair of chromosomes. A Bambara groundnut karyotype was constructed using these novel repeats along with ribosomal RNA genes (45S and 5S) and telomeric DNA sequences. This study provides the first analysis of the genome and chromosome properties of Bambara groundnut. We discuss our findings in relation to genetic improvement of Bambara groundnut and centromere evolution in legume species.

Genome ◽  
2011 ◽  
Vol 54 (11) ◽  
pp. 898-910 ◽  
Author(s):  
P. Somta ◽  
S. Chankaew ◽  
O. Rungnoi ◽  
P. Srinives

Bambara groundnut ( Vigna subterranea (L.) Verdc.) is an important African legume crop. In this study, a collection consisting of 240 accessions was analyzed using 22 simple sequence repeat (SSR) markers. In total, 166 alleles were detected, with a mean of 7.59 alleles per locus. Allelic and gene diversities were higher in the west African and Cameroon/Nigeria regions with 6.68 and 6.18 alleles per locus, and 0.601 and 0.571, respectively. The genetic distance showed high similarity between west African and Cameroon/Nigeria accessions. Principal coordinate analyses and neighbor-joining analysis consistently revealed that the majority of west African accessions were grouped with Cameroon/Nigeria accessions, but they were differentiated from east African, central African, and southeast Asian accessions. Population structure analysis showed that two subpopulations existed, and most of the east African accessions were restricted to one subpopulation with some Cameroon/Nigeria accessions, whereas most of the west African accessions were associated with most of the Cameroon/Nigeria accessions in the other subpopulation. Comparison with SSR analysis of other Vigna cultigens, i.e., cultivated azuki bean ( Vigna angularis ) and mungbean ( Vigna radiata ), reveals that the mean gene diversity of Bambara groundnut was lower than azuki bean but higher than mungbean.


2008 ◽  
Vol 44 (2) ◽  
pp. 167-183 ◽  
Author(s):  
A. SESAY ◽  
C. N. MAGAGULA ◽  
A. B. MANSUETUS

SUMMARYBambara groundnut (Vigna subterranea) is a grain legume crop, which is increasingly popular as food in rural areas across the African continent. However, reliable information pertaining to management of the crop is limited. Field experiments were conducted in Swaziland in the 1998/99 and 1999/2000 cropping seasons to determine the influence of sowing date and environmental factors on the growth, development and yield of bambara groundnut. In the 1998/99 season, seeds of one local landrace were sown on six dates between mid-September 1998 and mid-February 1999. In 1999/2000, seeds of two local landraces were sown on six dates between mid-October 1999 and late January 2000. The highest pod yield and total dry matter production were achieved in November sowings, with maximum pod yields of 1.3 and 0.64 t ha−1 for the 1998/99 and 1999/2000 seasons, respectively. Earlier sowing and successive delays in sowing from November caused substantial yield declines of as much as 72–75%. Sowing date influenced yields of bambara groundnut through the effect of temperature and daylength on plant development. For the landraces used in the study, while the rate of progress from sowing to flowering was influenced by temperature, the rate of progress from flowering to podding was influenced largely by daylength. The practical implications of the results for increased bambara groundnut production are discussed.


Author(s):  
Mahmudul Hasan ◽  
Md Kamal Uddin ◽  
Mahmud Tengku Muda Mohamed ◽  
Ali Tan Kee Zuan

Bambara groundnut (Vigna subterranea) has characteristics to grow in the marginal soil and also tolerant into the drought condition and also have potential of nitrogen fixation. Nitrogen is the key plant nutrient that stimulates root and shoot growth. Phosphorus application significantly improves many aspects of plant physiology including photosynthesis, flowering, fruiting and maturation which ultimately result in better yield. Symbiotic nitrogen fixation is a complex process, in which Rhizobium bacteria form a beneficial interaction with a legume crop to fix atmospheric nitrogen and convert it to ammonium for plant uptake. Biological N fixation (BNF) is the natural means to enhance soil fertility status and productivity. Application of nitrogen and phosphorus fertilizer exerted significant effect on root development, photosynthesis, yield contributing character and pod yield of the crop. Bambara Groundnut yields are low due to abiotic and biotic stresses. But with application of nitrogen and phosphorus increase the yield of this crop. It’s seeds contain 63 percent carbohydrate, 19 percent protein and 6.5 percent oil and good source of fibre, calcium, iron and potassium.


2017 ◽  
Vol 44 (3) ◽  
pp. 279
Author(s):  
Zikril Illahi ◽  
Ni Made Armini Wiendi ◽  
Dan Sudarsono

<em></em><em>ABSTRACT<br /><br />Bambara groundnut (Vigna subterranea L. Verdc.) is an important underutilized legume crop in Indonesia. The aim of this research is to study genetic diversity of bambara groundnut from Sukabumi and Sumedang, West Java, Indonesia. This study used 107 bambara groundnut accessions, which consisted of 57 accessions from Sukabumi and 50 accessions from Sumedang. We use five simple sequence repeat (SSR) to analyze the accessions. Totally nine alleles were detected, with a mean of 1.8 alleles per locus. Allelic and gene diversities were higher in Sumedang (1.8 alleles per locus and 0.119) than in Sukabumi population (1.4 alleles per locus and 0.020), respectively. We constructed a phylogenic tree by Neighbor-Joining analysis based on genetic distances (DA) and showed the tree divided bambara groundnut accessions into two broad groups according to the origin of samples (Sukabumi and Sumedang). Results from the phylogenic tree are in line with those from the population structure analysis.<br /> <br />Keywords: allele diversity, bambara groundnut, genetic distance, under utilized legume<br /><br /></em>


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Adré Minnaar-Ontong ◽  
Abe S. Gerrano ◽  
Maryke T. Labuschagne

AbstractWith its drought tolerant and protein-rich properties, Bambara groundnut [Vigna subterranea (L.) Verdc.], an indigenous African legume crop can contribute immensely to food security. This miracle crop is used as food and for the enhancement of soil fertility in South Africa. Knowledge on the genetic diversity and structure among the Bambara groundnut landraces can pave the way for the effective use and cultivation of this crop in southern Africa, especially South Africa. The aim of this study was to assess the genetic diversity and structure among Bambara groundnut landraces collected across South Africa and compared to a limited number of accessions from southern Africa using SSR markers. Seventy-eight Bambara groundnut accessions were genotyped using 19 Bambara specific SSR markers. SSR loci explored in this study, were all polymorphic. A total of 127 alleles were detected with a mean of 6.7 alleles per locus. Allele diversity and frequency among genotypes varied from 0.21 to 0.85 with an average of 0.62 per locus. Genetic variation as described by the analysis of molecular variance indicated higher genetic diversity (92%) within landraces than between (8%) different landraces. Population structure analysis showed that three subpopulations existed, and most of the South African accessions were restricted to one subpopulation, indicating that Bambara landraces has the ability to form unique haplotypes in different environments. Information harnessed in this study is helpful for further use in breeding programs for crop improvement.


2018 ◽  
Vol 30 (2) ◽  
pp. 19-28
Author(s):  
A. J. Oludare ◽  
J. I. Kioko ◽  
A. A. Akeem ◽  
A. T. Olumide ◽  
K. R. Justina ◽  
...  

Nine accessions of Bambara groundnut (Vigna subterranea (L.) Verdc.,syn. Voandzeia subterranea (L.) Thouars ex DC.)  obtained from National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan, Oyo state, were assessed for their genetic and phylogenetic relatedness through electrophoretic analysis of the seed proteins. 0.2g of the seeds were weighed and macerated with mortar and pestle in 0.2M phosphate buffer containing 0.133M of acid (NaH2PO4) and 0.067 of base (Na2HPO4) at pH 6.5. Protein characterization with standard marker revealed that the seeds of the nine accessions contained proteins (B.S.A, Oval Albumin, Pepsinogen, Trypsinogen and Lysozyme) with molecular weights ranging from 66kda and above, 45 – 65 kDa, 44 – 33 kda, 32-24 kDa and 23-14 kDa, respectively. The student T-test revealed that accessions B, C, E, F, H and I have molecular weights not significantly different from one another (P<0.05) while samples A, D and G showed significantly different values (P>0.05). All the accessions had at least two proteins and two major bands in common. The study revealed intra-specific similarities and genetic diversity in protein contents among the nine accessions of Bambara groundnut (Vigna subterraranea (L.) Verdc.syn


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Daniela Prušáková ◽  
Vratislav Peska ◽  
Stano Pekár ◽  
Michal Bubeník ◽  
Lukáš Čížek ◽  
...  

AbstractTelomeres are protective structures at the ends of eukaryotic chromosomes, and disruption of their nucleoprotein composition usually results in genome instability and cell death. Telomeric DNA sequences have generally been found to be exceptionally conserved in evolution, and the most common pattern of telomeric sequences across eukaryotes is (TxAyGz)n maintained by telomerase. However, telomerase-added DNA repeats in some insect taxa frequently vary, show unusual features, and can even be absent. It has been speculated about factors that might allow frequent changes in telomere composition in Insecta. Coleoptera (beetles) is the largest of all insect orders and based on previously available data, it seemed that the telomeric sequence of beetles varies to a great extent. We performed an extensive mapping of the (TTAGG)n sequence, the ancestral telomeric sequence in Insects, across the main branches of Coleoptera. Our study indicates that the (TTAGG)n sequence has been repeatedly or completely lost in more than half of the tested beetle superfamilies. Although the exact telomeric motif in most of the (TTAGG)n-negative beetles is unknown, we found that the (TTAGG)n sequence has been replaced by two alternative telomeric motifs, the (TCAGG)n and (TTAGGG)n, in at least three superfamilies of Coleoptera. The diversity of the telomeric motifs was positively related to the species richness of taxa, regardless of the age of the taxa. The presence/absence of the (TTAGG)n sequence highly varied within the Curculionoidea, Chrysomeloidea, and Staphylinoidea, which are the three most diverse superfamilies within Metazoa. Our data supports the hypothesis that telomere dysfunctions can initiate rapid genomic changes that lead to reproductive isolation and speciation.


2014 ◽  
Vol 955-959 ◽  
pp. 419-422
Author(s):  
Gui Lin Liu ◽  
Yan Ping Ding ◽  
Yan Ling Wu ◽  
Wen Zhang

Telomeric DNA of human chromosomes plays a significant role in physiological processes such as cell cycle, aging, cancer and genetic stability due to its special sequence and structure. The research on small molecule ligands targeting G-quadruplex formed by such special sequence has attracted considerable attention, and has achieved great breakthrough. In this paper, we summarize the DNA sequences and structures of three kinds of typical human telomeric G-quadruplex, providing an important reference for further research.


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