MEIOSIS IN DIABROTICA (COLEOPTERA: CHRYSOMELIDAE): CHIASMA FREQUENCY AND VARIATION

1972 ◽  
Vol 14 (1) ◽  
pp. 113-128 ◽  
Author(s):  
Terry Ennis

Fifteen species of Diabrotica have been examined cytologically. Except for the variable presence of supernumeraries in three of the species, the karyotype is uniform throughout, with 18 meta- or submetacentric autosomes and an XX ♂: XO ♀ sex-determining mechanism. Chiasma frequency is quite variable; in addition to the basic nine per cell, formation of a second chiasma (in the other arm) results in ring frequencies ranging from zero to nine per cell. Analysis of chiasma frequency and distribution at diakinesis and metaphase reveals that the rings are bi-chiasmate and not the result of ectopic pairing. With this in mind, chiasma formation is discussed in terms of interference. Intra-arm interference is complete; the upper limit of chiasmata per arm is invariably one. The strength of interference across the centromere determines the probability of formation of a chiasma in the other arm. When interference is strong, a second chiasma is rarely formed; as interference weakens, a chiasma is more easily formed in the other arm, resulting in a higher chiasma frequency. Associated with decreasing interference and an elevated chiasma frequency is an increase in cell variance; chiasma formation is less rigorously controlled, and variability in the number of rings per cell increases. The variability of ring formation in the genus militates against distinguishing taxa on this basis. However, the importance of increased chiasma frequency (coupled as it is with greater variance) resides in its influence on the genetic plasticity and adaptive potential of the species.

Genetics ◽  
2002 ◽  
Vol 161 (1) ◽  
pp. 307-314 ◽  
Author(s):  
J King ◽  
L A Roberts ◽  
M J Kearsey ◽  
H M Thomas ◽  
R N Jones ◽  
...  

Abstract A single chromosome of the grass species Festuca pratensis has been introgressed into Lolium perenne to produce a diploid monosomic substitution line (2n = 2x = 14). The chromatin of F. pratensis and L. perenne can be distinguished by genomic in situ hybridization (GISH), and it is therefore possible to visualize the substituted F. pratensis chromosome in the L. perenne background and to study chiasma formation in a single marked bivalent. Recombination occurs freely in the F. pratensis/L. perenne bivalent, and chiasma frequency counts give a predicted map length for this bivalent of 76 cM. The substituted F. pratensis chromosome was also mapped with 104 EcoRI/Tru91 and HindIII/Tru91 amplified fragment length polymorphisms (AFLPs), generating a marker map of 81 cM. This map length is almost identical to the map length of 76 cM predicted from the chiasma frequency data. The work demonstrates a 1:1 correspondence between chiasma frequency and recombination and, in addition, the absence of chromatid interference across the Festuca and Lolium centromeres.


2021 ◽  
Vol 647 ◽  
pp. L3 ◽  
Author(s):  
J. Cernicharo ◽  
C. Cabezas ◽  
M. Agúndez ◽  
B. Tercero ◽  
N. Marcelino ◽  
...  

We present the discovery in TMC-1 of allenyl acetylene, H2CCCHCCH, through the observation of nineteen lines with a signal-to-noise ratio ∼4–15. For this species, we derived a rotational temperature of 7 ± 1 K and a column density of 1.2 ± 0.2 × 1013 cm−2. The other well known isomer of this molecule, methyl diacetylene (CH3C4H), has also been observed and we derived a similar rotational temperature, Tr = 7.0 ± 0.3 K, and a column density for its two states (A and E) of 6.5 ± 0.3 × 1012 cm−2. Hence, allenyl acetylene and methyl diacetylene have a similar abundance. Remarkably, their abundances are close to that of vinyl acetylene (CH2CHCCH). We also searched for the other isomer of C5H4, HCCCH2CCH (1.4-Pentadiyne), but only a 3σ upper limit of 2.5 × 1012 cm−2 to the column density can be established. These results have been compared to state-of-the-art chemical models for TMC-1, indicating the important role of these hydrocarbons in its chemistry. The rotational parameters of allenyl acetylene have been improved by fitting the existing laboratory data together with the frequencies of the transitions observed in TMC-1.


Synthesis ◽  
2019 ◽  
Vol 51 (09) ◽  
pp. 1923-1934 ◽  
Author(s):  
Jean Rodriguez ◽  
Adrien Quintard

In order to circumvent reactivity or selectivity issues associated with the addition of enolates to electrophiles, chemists have devised innovative methods involving transient activating groups. One of these powerful methods consists of the use of activated ketones, such as α-nitroketones, β-dicarbonyl compounds or β-ketosulfones, with electrophiles possessing a latent hydroxy or amine function. In the presence of a suitable catalyst, an enantioselective addition to the electrophile is facilitated triggering a subsequent Claisen-type fragmentation resulting in an acyl transfer. This subsequent step unveils the desired mono-activated function while directly transferring the ketone, forming in situ on the other side an ester or an amide.1 Introduction2 Intramolecular Acyl Transfer with Acyclic Substrates2.1 Bifunctional Catalysis2.2 Aminocatalysis3 Intermolecular Acyl Transfer with Acyclic Substrates4 Medium-Sized-Ring Formation with Cyclic Substrates5 Conclusion


Genome ◽  
1987 ◽  
Vol 29 (2) ◽  
pp. 292-308 ◽  
Author(s):  
B. John

The existing data on the behaviour of multiple chromosome configurations arising from single interchanges between either metacentric–telocentric or telocentric–telocentric nonhomologues in 10 species of acridid grasshoppers are compared with data from four new cases. Two of these new cases involve metacentric–telocentric exchanges but the other two, for the first time in acridids, deal with a reciprocal translocation between two nonhomologous metacentrics. The combined data are used to evaluate the factors that influence multiple orientation in this family of grasshoppers and reemphasize the importance of chiasma frequency and chiasma distribution for multiple behaviour. This conclusion is reinforced by a consideration of the known cases of chain of three multiples originating from the Robertsonian fusion of nonhomologous telocentrics in acridoids. Key words: acridid grasshoppers, multiple chromosome configurations, chiasma distribution, orientation behaviour.


Genome ◽  
1992 ◽  
Vol 35 (4) ◽  
pp. 653-658 ◽  
Author(s):  
M. M. Kruleva ◽  
A. B. Korol ◽  
T. G. Dankov ◽  
V. G. Skorpan ◽  
I. A. Preygel

The effect of four isogenic cytoplasmic types, normal, Salvador, Texas, and Charrua (the latter three causing male sterility), on the process of chiasma formation has been studied using two different maize hybrids. The cytoplasmic male sterility determinants have been shown to reduce the rate of interstitial exchanges per nucleus and per bivalent and the frequency of univalents. Increased variation between plants and relative stability of the intercellular variation within a plant have been observed for the parameters studied. It is concluded that the determinants of cytoplasmic sterility lower the probability of additional exchanges (relative to the obligate one) and reduce the frequency of premature disruption of one-exchange chromosome associations.Key words: maize, chiasma frequency, male sterile cytoplasm, univalent formation, genotype × cytoplasm interaction.


1999 ◽  
Vol 08 (04) ◽  
pp. 507-517 ◽  
Author(s):  
DEEPAK JAIN ◽  
N. PANCHAPAKESAN ◽  
S. MAHAJAN ◽  
V. B. BHATIA

Identification of gravitationally lensed Gamma Ray Bursts (GRBs) in the BATSE 4B catalog can be used to constrain the average redshift <z> of the GRBs. In this paper we investigate the effect of evolving lenses on the <z> of GRBs in different cosmological models of the universe. The cosmological parameters Ω and Λ have an effect on the <z> of GRBs. The other factor which can change the <z> is the evolution of galaxies. We consider three evolutionary model of galaxies. In particular, we find that the upper limit on <z> of GRBs is higher in evolving model of galaxies as compared to non-evolving models of galaxies.


Genome ◽  
1995 ◽  
Vol 38 (3) ◽  
pp. 616-622 ◽  
Author(s):  
A. L. del Cerro ◽  
J. L. Santos

Analysis of surface-spread synaptonemal complexes of zygotene and pachytene spermatocytes was carried out on centric-shift heterozygotes of grasshoppers. These rearrangements affected the M7 chromosome in Chorthippus vagans and the M6 and S8 chromosomes in Chorthippus apricarius. The shifts in the latter two chromosomes were also associated with C-heterochromatin variations between homologous chromosomes. Rearranged chromosomes proceeded directly to heterosynapsis without an apparent intervening homosynaptic phase in M7 bivalents of Ch. vagans and M6 bivalents of Ch. apricarius. In the latter case, axial equalization of the heterochromatin polymorphism was also achieved. On the other hand, asynapsis of the intercentromeric regions throughout pachytene was the rule in the centric shift involving the S8 chromosome of Ch. apricarius. In the three cases analysed, the production of unbalanced gametes in the heterozygotes is precluded either by the lack of chiasma formation in heterosynapsed rearranged segments or by the lack of pairing between such segments. Chiasmata were limited to the homologous regions of the heteromorphic bivalents.Key words: synapsis, surface spreading, centric shift, chiasma distribution, meiosis.


1984 ◽  
Vol 26 (3) ◽  
pp. 264-271 ◽  
Author(s):  
Kunio Kitada ◽  
Takeshi Omura

One complete asynaptic mutant, MM-19, and two partial ones, MM-4 and MM-16, of Oryza sativa L. induced by N-methyl-N-nitrosourea (MNU) were cytogenetically investigated. No chromosome pairing occurred from zygotene to pachytene and 24 univalents appeared at diakinesis and metaphase 1 in MM-19. On the other hand, a partial lack of chromosome pairing was observed from zygotene to pachytene and various numbers of univalents occurred at metaphase I in MM-4 and MM-16. The mean chiasma frequency per bivalent as well as per cell decreased to different extents in MM-4 and MM-16, and the correlation between both the amount of chromosome pairing from zygotene to pachytene and the chiasma frequency per cell at diakinesis was recognized. Judging from the development of anthers in each meiotic stage, the duration of the stage forming the synizetic knot, at which chromosome pairing took place, was longer in MM-4 and MM-16 than in the normal plant, and was in MM-19 almost as long as in the normal plant. The results of gene analyses indicate that each of the three asynaptic mutants is controlled by a recessive gene and that, at least for MM-4 and MM-16, these genes are located at different loci.Key words: asynaptic, rice, Oryza, chiasma frequency, synizesis.


Author(s):  
Teruyuki Kitabayashi

We study the effect of the scotogenic dark matter on the primordial black holes (PBHs) and vice versa. We show that if the PBHs evaporate in the radiation dominated era, the upper limit of the initial mass of the PBHs [Formula: see text] should be constrained as [Formula: see text] for [Formula: see text] TeV scotogenic dark matter [Formula: see text] TeV is the most appropriate energy scale in the scotogenic model). On the other hand, if the PBHs evaporate in the PBH dominated era, a quite heavy scotogenic dark matter ([Formula: see text] GeV) for [Formula: see text] may be allowed.


2020 ◽  
Vol 643 ◽  
pp. A31
Author(s):  
A. Kotrlová ◽  
E. Šrámková ◽  
G. Török ◽  
K. Goluchová ◽  
J. Horák ◽  
...  

We explore the influence of nongeodesic pressure forces present in an accretion disc on the frequencies of its axisymmetric and nonaxisymmetric epicyclic oscillation modes. We discuss its implications for models of high-frequency quasi-periodic oscillations (QPOs), which have been observed in the X-ray flux of accreting black holes (BHs) in the three Galactic microquasars, GRS 1915+105, GRO J1655−40, and XTE J1550−564. We focus on previously considered QPO models that deal with low-azimuthal-number epicyclic modes, |m| ≤ 2, and outline the consequences for the estimations of BH spin, a ∈ [0, 1]. For four out of six examined models, we find only small, rather insignificant changes compared to the geodesic case. For the other two models, on the other hand, there is a significant increase of the estimated upper limit on the spin. Regarding the falsifiability of the QPO models, we find that one particular model from the examined set is incompatible with the data. If the spectral spin estimates for the microquasars that point to a >  0.65 were fully confirmed, two more QPO models would be ruled out. Moreover, if two very different values of the spin, such as a ≈ 0.65 in GRO J1655−40 and a ≈ 1 in GRS 1915+105, were confirmed, all the models except one would remain unsupported by our results. Finally, we discuss the implications for a model that was recently proposed in the context of neutron star (NS) QPOs as a disc-oscillation-based modification of the relativistic precession model. This model provides overall better fits of the NS data and predicts more realistic values of the NS mass compared to the relativistic precession model. We conclude that it also implies a significantly higher upper limit on the microquasar’s BH spin (a ∼ 0.75 vs. a ∼ 0.55).


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