Indistinguishable patterns of recombination resulting from male and female meioses in Brassica napus (oilseed rape)

Genome ◽  
1997 ◽  
Vol 40 (1) ◽  
pp. 49-56 ◽  
Author(s):  
A. L. Kelly ◽  
A. G. Sharpe ◽  
J. H. Nixon ◽  
D. J. Lydiate ◽  
E. J. Evans
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Genetic Maps ◽  
Linkage Groups ◽  
Male Population ◽  
Female Population ◽  
Male And Female ◽  
Male Gametes ◽  
Genome Map ◽  
Backcross Populations

An F1 individual derived from a cross between two distinct lines of spring oilseed rape (Brassica napus) was used to produce a pair of complementary backcross populations, each consisting of 90 individuals. The F1 donated male gametes to the Male population and female gametes to the Female population. Genetic maps were generated from both populations and aligned using 117 common loci to form an integrated genome map of B. napus with 243 RFLP-defined loci. A comparison of the frequency and distribution of crossovers in the two populations of F1 gametes (assayed in the Male and Female populations) detected no differences. The genetic maps derived from the Male and Female populations each consisted of 19 linkage groups spanning 1544 and 1577 cM, respectively. The maps were aligned with other B. napus maps, and all 19 equivalent linkage groups were unambiguously assigned. The genetic size and general organisation of the new maps were comparable with those of pre-existing B. napus maps in most respects, except that the levels of polymorphism in the constituent A and C genomes were unusually similar in the new cross.Key words: genetic linkage map, sex differences, recombination frequency, segregation distortion.

Homoeologous loci control the accumulation of seed glucosinolates in oilseed rape (Brassica napus)

Genome ◽  
2003 ◽  
Vol 46 (3) ◽  
pp. 454-460 ◽  
Author(s):  
P M Howell ◽  
A G Sharpe ◽  
D J Lydiate
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Phenotypic Variation ◽  
Comparative Mapping ◽  
Genetic Maps ◽  
Glucosinolate Content ◽  
Marker Assisted Breeding ◽  
Brassica Napus L ◽  
Backcross Populations ◽  
Seed Glucosinolates

The genetic control of seed glucosinolate content in oilseed rape was investigated using two intervarietal backcross populations. Four QTLs segregating in the population derived from a Brassica napus L.'Victor' × Brassica napus L.'Tapidor' cross, together accounting for 76% of the phenotypic variation, were mapped. Three of these loci also appeared to control the accumulation of seed glucosinolates in a Brassica napus L. 'Bienvenu' × 'Tapidor' cross, and accounted for 86% of the phenotypic variation. The three QTLs common to both populations mapped to homoeologous regions of the B. napus genome, suggesting that seed glucosinolate accumulation is controlled by duplicate genes. It was possible to extend the comparative analysis of QTLs controlling seed glucosinolate accumulation by aligning the published genetic maps generated by several research groups. This comparative mapping demonstrated that high-glucosinolate varieties often carry low-glucosinolate alleles at one or more of the loci controlling seed glucosinolate accumulation.Key words: backcross, comparative mapping, genetic linkage map, marker-assisted breeding, QTL.

scholarly journals Population challenges from the gender perspective

2010 ◽  
pp. 23-32
Author(s):  
Ankica Sobot
Keyword(s):  
Gender Gap ◽  
Social Care ◽  
Comparative Approach ◽  
Gender Imbalance ◽  
Demographic Development ◽  
Male Population ◽  
Female Population ◽  
Male And Female ◽  
Never Married ◽  
Demographic Processes

In this paper, population challenges are recognized from the perspective of various demographic structures of the male and female population. The larger scope of female population in Vojvodina is mainly the result of a more emphasized gender imbalance among the population of 65 or over. There are more unmarried females but more males among the never-married persons. Among the middle-aged population, there are more women with higher education, but they are also economically independent to a greater extent than the male population. Gender equality is a challenge as a factor of demographic processes and as an aspect of policies directed towards the population, but adequate responses for demographic development and social care of the population are implied by both male and female positions. By comparative approach, the characteristics of gender positions and differences in Vojvodina are noted, as well as the space for promoting positions of sexes and mitigation of the gender gap. The necessity for a deeper socio-demographic analysis and empirical research of gender differences as a relevant population challenge are also noted. .

scholarly journals A Modified Meiotic Recombination in Brassica napus Largely Improves Its Breeding Efficiency

Biology ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 771
Author(s):  
Franz Boideau ◽  
Alexandre Pelé ◽  
Coleen Tanguy ◽  
Gwenn Trotoux ◽  
Frédérique Eber ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Meiotic Recombination ◽  
Main Tool ◽  
Genetic Maps ◽  
Deleterious Mutations ◽  
Ploidy Levels ◽  
Per Se ◽  
Breeding Efficiency ◽  
Reference Genomes

Meiotic recombination is the main tool used by breeders to generate biodiversity, allowing genetic reshuffling at each generation. It enables the accumulation of favorable alleles while purging deleterious mutations. However, this mechanism is highly regulated with the formation of one to rarely more than three crossovers, which are not randomly distributed. In this study, we showed that it is possible to modify these controls in oilseed rape (Brassica napus, AACC, 2n = 4x = 38) and that it is linked to AAC allotriploidy and not to polyploidy per se. To that purpose, we compared the frequency and the distribution of crossovers along A chromosomes from hybrids carrying exactly the same A nucleotide sequence, but presenting three different ploidy levels: AA, AAC and AACC. Genetic maps established with 202 SNPs anchored on reference genomes revealed that the crossover rate is 3.6-fold higher in the AAC allotriploid hybrids compared to AA and AACC hybrids. Using a higher SNP density, we demonstrated that smaller and numerous introgressions of B. rapa were present in AAC hybrids compared to AACC allotetraploid hybrids, with 7.6 Mb vs. 16.9 Mb on average and 21 B. rapa regions per plant vs. nine regions, respectively. Therefore, this boost of recombination is highly efficient to reduce the size of QTL carried in cold regions of the oilseed rape genome, as exemplified here for a QTL conferring blackleg resistance.

scholarly journals Determinants and Prevalence of Metabolic Syndrome in Subjects Attending the General Outpatient Department of the Federal Medical Centre Katsina State, North West Nigeria

2020 ◽  
pp. 28-37
Author(s):  
A. I. Yaradua ◽  
A. J. Alhassan ◽  
M. K. Atiku ◽  
M. A. Wudil ◽  
K. I. Matazu ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Intervention Program ◽  
Medical Center ◽  
Individual Risk ◽  
Normal Range ◽  
Anthropometric Parameters ◽  
Male Population ◽  
Female Population ◽  
Male And Female ◽  
North West

Metabolic Syndrome (MS) is a term that describe the presence of conditions that increase an individual risk for heart disease and other disorders such as diabetes and stroke, and its occurrence is in the rise in residents of North-Western Nigeria, The aim of this study was to investigate the prevalence and determinants of metabolic syndrome in respondents from Katsina senatorial zone attending the Out-patient Department of the Federal Medical Center Katsina, Katsina State, Nigeria. A total of 211 (male 109; female 102) respondents aged 10 to 80 years were recruited for the study. Anthropometric parameters and blood pressure of respondents were determined using standard methods; serum lipid profile was determined using enzymatic methods. From the results, 11.8% of the male respondents were under weight, 51.6% were within the normal range, 25.4% of the study male population was found to be overweight and 12.8% were obese. 13.5% of the female respondents were under weight, 51.9% were within the normal range, 22.8% of the study female population was found to be overweight and 13.1% were obese. All serum lipids measurements with the exception of serum LDL-C concentration correlated positively with age. There were no statistically significant differences between the frequencies of total cholesterol, HDL, LDL and TRIG between the male and female respondents. The most common form of Dyslipidemia in the male and female respondents is low HDL-C. BMI, SBP, LDL-C, HDLC and TRIG were associated metabolic syndrome with the association being significant for SBP and HDL-C (0.05; 0.03) in the male respondent, in the female respondents BMI, SBP, DBP, LDL-C and TRIG were associated metabolic syndrome with the association being significant for SBP, DBP, and TRIG (0.04; 0.04; 0.04) respectively. The prevalence of metabolic syndrome was highest in male (31.75%) than in the female respondents (28.33).The prevalence of metabolic syndrome is common in the population under study with the male respondents having the highest prevalence. A robust and well design intervention program by concerned authorities is desirable to address complications of the risk factors for metabolic syndrome in the population.

Frequent nonreciprocal translocations in the amphidiploid genome of oilseed rape (Brassica napus)

Genome ◽  
1995 ◽  
Vol 38 (6) ◽  
pp. 1112-1121 ◽  
Author(s):  
A. G. Sharpe ◽  
I. A. P. Parkin ◽  
D. J. Keith ◽  
D. J. Lydiate
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Doubled Haploid ◽  
Microspore Culture ◽  
Homoeologous Recombination ◽  
Linkage Groups ◽  
Rflp Map ◽  
A Genome ◽  
C Genome ◽  
Marker Loci

A RFLP map of Brassica napus, consisting of 277 loci arranged in 19 linkage groups, was produced from genetic segregation in a combined population of 174 doubled-haploid microspore-derived lines. The integration of this map with a B. napus map derived from a resynthesized B. napus × oilseed rape cross allowed the 10 linkage groups of the B. napus A genome and the 9 linkage groups of the C genome to be identified. Collinear patterns of marker loci on different linkage groups suggested potential partial homoeologues. RFLP patterns consistent with aberrant chromosomes were observed in 9 of the 174 doubled-haploid lines. At least 4 of these lines carried nonreciprocal, homoeologous translocations. These translocations were probably the result of homoeologous recombination in the amphidiploid genome of oilseed rape, suggesting that domesticated B. napus is unable to control chromosome pairing completely. Evidence for genome homogenization in oilseed rape is presented and its implications on genetic mapping in amphidiploid species is discussed. The level of polymorphism in the A genome was higher than that in the C genome and this might be a general property of oilseed rape crosses.Key words: restriction fragment length polymorphism, genetic linkage map, homoeologous recombination, microspore culture, doubled haploid.

scholarly journals Identification of Quantitative Trait Loci for Resistance Against Verticillium longisporum in Oilseed Rape (Brassica napus)

2008 ◽  
Vol 98 (2) ◽  
pp. 215-221 ◽  
Author(s):  
W. Rygulla ◽  
R. J. Snowdon ◽  
W. Friedt ◽  
I. Happstadius ◽  
W. Y. Cheung ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Quantitative Trait Loci ◽  
Oilseed Rape ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Quantitative Trait ◽  
Fragment Length Polymorphism ◽  
Linkage Groups ◽  
Verticillium Longisporum ◽  
Dh Lines

Verticillium longisporum is one of the major pathogens of oilseed rape (Brassica napus; genome AACC, 2n = 38) in Europe. Current European cultivars possess only a low level of resistance against V. longisporum, meaning that heavy infection can cause major yield losses. The aim of this study was to identify quantitative trait loci (QTL) for resistance against V. longisporum as a starting point for marker-assisted breeding of resistant cultivars. Resistance QTL were localized in a segregating oilseed rape population of 163 doubled haploid (DH) lines derived by microspore culture from the F1 of a cross between two B. napus breeding lines, one of which exhibited V. longisporum resistance derived by pedigree selection from a resynthesized B. napus genotype. A genetic map was constructed comprising 165 restriction fragment length polymorphism, 94 amplified fragment length polymorphism and 45 simple sequence repeats (SSR) markers covering a total of 1,739 cM on 19 linkage groups. Seedlings of the DH lines and parents were inoculated with V. longisporum isolates in four greenhouse experiments performed in Sweden during autumn 1999. In three of the experiments the DH lines were inoculated with a mixture of five isolates, while in the fourth experiment only one of the isolates was used. The intention was to simulate four different environments with variable disease pressure, while still maintaining uniform conditions in each environment to enable reliable disease scoring. The disease index (DI) was calculated by scoring symptoms on a total of 21 inoculated plants per line in comparison to 21 noninoculated plants per line. Using the composite interval mapping procedure a total of four different chromosome regions could be identified that showed significant QTL for resistance in more than one environment. Two major QTL regions were identified on the C-genome linkage groups N14 and N15, respectively; each of these QTL consistently exhibited significant effects on resistance in multiple environments. The presence of flanking markers for the respective QTL was associated with a significant reduction in DI in the inoculated DH lines.

Conserved patterns of chromosome pairing and recombination in Brassica napus crosses

Genome ◽  
1997 ◽  
Vol 40 (4) ◽  
pp. 496-504 ◽  
Author(s):  
I. A. P. Parkin ◽  
D. J. Lydiate
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Chromosome Pairing ◽  
Recombination Frequency ◽  
Microspore Culture ◽  
Genetic Maps ◽  
Winter Oilseed Rape ◽  
Homologous Chromosomes ◽  
Novel Alleles ◽  
Two Populations

The patterns of chromosome pairing and recombination in two contrasting Brassica napus F1 hybrids were deduced. One hybrid was from a winter oilseed rape (WOSR) × spring oilseed rape cross, the other from a resynthesized B. napus × WOSR cross. Segregation at 211 equivalent loci assayed in the population derived from each hybrid produced two collinear genetic maps. Alignment of the maps indicated that B. napus chromosomes behaved reproducibly as 19 homologous pairs and that the 19 distinct chromosomes of B. napus each recombined with unique chromosomes from the interspecific hybrid between Brassica rapa and Brassica oleracea. This result indicated that the genomes of the diploid progenitors of amphidiploid B. napus have remained essentially unaltered since the formation of the species and that the progenitor genomes were similar to those of modern-day B. rapa and B. oleracea. The frequency and distribution of crossovers were almost indistinguishable in the two populations, suggesting that the recombination machinery of B. napus could cope easily with different degrees of genetic divergence between homologous chromosomes. Efficient recombination in wide crosses will facilitate the introgression of novel alleles into oilseed rape from B. rapa and B. oleracea (via resynthesized B. napus) and reduce linkage drag.Key words: integrating genetic maps, microspore culture, segregation distortion, recombination frequency, locus distribution.

scholarly journals Theoretical and practical consequences of imbalance in the coefficient of male and female population in modern Russia: impact of the factor of ratio of genders in the region upon individual level of financial wellbeing

2020 ◽  
pp. 30-45
Author(s):  
Olga Vladimirovna Semenova ◽  
Marina L'vovna Butovskaya
Keyword(s):  
Quantitative Research ◽  
Negative Impact ◽  
Subjective Assessment ◽  
Dispersion Analysis ◽  
Modern Concept ◽  
Male Population ◽  
Female Population ◽  
Male And Female ◽  
Individual Level ◽  
Parental Investments

This article presents a brief overview of the modern concept of gender selection and theory of parental investments, according to which the ration of genders in society manifests as a key forecaster of the level of polygyny on the marital market. The theory predicts that in shifting of the models of marital behavior from monogamous and polygamous, the level of matrimonial investment can decrease. The authors suggest that such targeted decrease of resources in the regions with low coefficient of genders can exert negative impact on the economic parameters of household in these regions. This research provides quantitative assessment of subjective satisfaction with financial position of the members of households in populated areas (N=10385) with various levels of coefficient of genders. The statistical analysis was conducted using the IBM SPSS software with application of the following methods: Pearson's χ2 criterion, Goodman and Kruskal's gamma (γ) criterion, and dispersion analysis. The acquired results confirm the presence of positive interconnection between the level of coefficient of genders in the region and financial wellbeing households. It was determined that in the regions of the Russian Federation with equal ration of male and female population, as well as in regions with predominant male population, the subjective assessment of own financial wellbeing was higher among both, men and women. The factor of sustained imbalance in the ratio of genders represents one of the relevant questions of Russian demography, but this phenomenon is rarely taken into consideration in quantitative research within social sciences.

scholarly journals SYSTEMIC INFLAMMATORY RESPONSE SYNDROME (SIRS)

2015 ◽  
Vol 22 (03) ◽  
pp. 293-298
Author(s):  
Shuaib Ansari ◽  
Irfan Murtaza Shahwani ◽  
Zeeshan Ali ◽  
Syed Zulfiquar Ali Shah ◽  
Faisal Shahab
Keyword(s):  
Liver Cirrhosis ◽  
Systemic Inflammatory Response Syndrome ◽  
Inflammatory Response ◽  
Systemic Inflammatory Response ◽  
University Hospital ◽  
Male Population ◽  
Cross Sectional ◽  
Female Population ◽  
Male And Female ◽  
The Mean

Systemic inflammatory response syndrome (SIRS) is a generalized disorder,immune response to infection which results in disturbed microcirculation, visceral perfusion andultimately visceral failure. Objectives: To determine the frequency of systemic inflammatoryresponse syndrome in patients with liver cirrhosis. Design: Cross sectional descriptive.Period: Six months study. Setting: At Liaquat University Hospital Hyderabad. Patients andmethods: All the patients with liver cirrhosis for 06 months duration admitted in the ward werefurther evaluated for systemic inflammatory response syndrome. The data was analyzed inSPSS 16 and the frequency and percentage was calculated. Results: During six months studyperiod, total 100 cirrhotic subjects were studied for SIRS. The mean ± SD for age in all (100)cirrhotic patients was 45.74 ± 7.537. The mean temperatures was 40.42±0.32 where as it was39.72±0.43 and 38.92±0.11 in male and female population respectively. The mean heart beatwas 128.42±6.74 where as it was 115.83±8.93 and 120.62±5.53 in male and female populationrespectively. The mean respiratory rate was 25.31±3.52 where as it was 23.52±2.31 and26.63±3.21 in male and female population respectively. The mean white blood cell count was28.81±4.51 where as it was 23.74±4.73 and 30.83±5.73 respectively. The SIRS was observedin 70% subjects of which 47(67.1%) were males and 23(32.9%) were females (p=0.04). Majorityof SIRS subjects were 30-39 years of age and male population was predominant (p=0.03). Thegender distribution in relation to severity of liver disease was statistically significant (p=0.05)while the SIRS in relation statical analysis. Conclusions: The systemic inflammatory responsesyndrome occurs in patients with liver cirrhosis.

scholarly journals Comparison of Mortality by Gender and Regions in Tanzania using Direct Standardized Death Rates (DSDR) Method

2020 ◽  
Vol 21 (1) ◽  
pp. 1-7
Author(s):  
Shadrack E. Kibona ◽  
Christopher H. Mbotwa
Keyword(s):  
Health Status ◽  
National Bureau ◽  
Male Population ◽  
Female Population ◽  
Death Rates ◽  
Male And Female ◽  
And Gender ◽  
Almost All ◽  
Direct Standardization ◽  
Better Than

Background: Comparison of mortality is very useful in assessing population health. Crude rates can easily be computed from the mortality but they are not good for comparison across groups of the population. The aim of this paper was to compare mortality in Tanzania by region and gender using the 2012 Tanzania Population and Housing Census. Methods: Age-specific death rates for regions, Tanzania Mainland, Tanzania Zanzibar and entire Tanzania were obtained from Mortality and Health monograph data downloadable from the National Bureau of Statistics website. The direct standardization method was used to compare the mortality for male and female populations across all regions of Tanzania Mainland and Zanzibar. Results: Findings show that the mortality is low in Arusha, Manyara, and Kilimanjaro compared to other regions in Tanzania mainland implying that health status for both male and female population in those regions is better than the other regions while it is the worst in Njombe, Iringa, and Kagera implying the poor health status for those regions as compared to the rest regions in Tanzania Mainland. In Tanzania Zanzibar, high mortality was observed in Kusini Unguja and the lowest in Kaskazini Unguja for both male and female populations. By national wise and for almost all regions, the mortality for the male population is higher than that for the female population. Conclusion: Direct standardization methods can save as the best way for comparing mortality because it takes into consideration both the population at risk and the age structure. However, in estimating mortality, crude death rates should be used to give the magnitude while direct standardized death rates should be used for comparison purposes.

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