The relationship between genetic and cytogenetic maps of pea. II. Physical maps of linkage mapping populations

Genome ◽  
1997 ◽  
Vol 40 (5) ◽  
pp. 755-769 ◽  
Author(s):  
K. J. Hall ◽  
J. S. Parker ◽  
T. H. N. Ellis ◽  
L. Turner ◽  
M. R. Knox ◽  
...  
Keyword(s):  
Linkage Map ◽  
Linkage Mapping ◽  
Molecular Data ◽  
Inbred Lines ◽  
Genetic Maps ◽  
Physical Maps ◽  
Structural Differences ◽  
Parental Lines ◽  
The Relationship ◽  
Mapping Populations

A cytogenetic analysis of inbred lines that have been used to generate genetic maps of pea is presented. Mitotic karyotyping of the inbred lines and meiotic studies of their F1 hybrids have been used to test the prediction that structural differences exist between the parental lines. The results are not compatible with the previously published molecular data. A reordered and updated linkage map of pea is presented that is consistent with the cytogenetic data.Key words: Pisum, linkage map, recombination, synaptonemal complex, chiasmata.

scholarly journals Heterogeneity in Rates of Recombination Across the Mouse Genome

Genetics ◽  
1996 ◽  
Vol 142 (2) ◽  
pp. 537-548 ◽  
Author(s):  
Michael W Nachman ◽  
Gary A Churchill
Keyword(s):  
Linkage Map ◽  
Genetic Map ◽  
Large Scale ◽  
Physical Map ◽  
Genetic Maps ◽  
Recombination Rates ◽  
Physical Maps ◽  
Genomic Patterns ◽  
Mary Lyon ◽  
Microsatellite Linkage

Abstract If loci are randomly distributed on a physical map, the density of markers on a genetic map will be inversely proportional to recombination rate. First proposed by MARY LYON, we have used this idea to estimate recombination rates from the Drosophila melanogaster linkage map. These results were compared with results of two other studies that estimated regional recombination rates in D. melanogaster using both physical and genetic maps. The three methods were largely concordant in identifying large-scale genomic patterns of recombination. The marker density method was then applied to the Mus musculus microsatellite linkage map. The distribution of microsatellites provided evidence for heterogeneity in recombination rates. Centromeric regions for several mouse chromosomes had significantly greater numbers of markers than expected, suggesting that recombination rates were lower in these regions. In contrast, most telomeric regions contained significantly fewer markers than expected. This indicates that recombination rates are elevated at the telomeres of many mouse chromosomes and is consistent with a comparison of the genetic and cytogenetic maps in these regions. The density of markers on a genetic map may provide a generally useful way to estimate regional recombination rates in species for which genetic, but not physical, maps are available.

scholarly journals QTL Analysis of Five Morpho-Physiological Traits in Bread Wheat Using Two Mapping Populations Derived from Common Parents

Genes ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 604
Author(s):  
Paolo Vitale ◽  
Fabio Fania ◽  
Salvatore Esposito ◽  
Ivano Pecorella ◽  
Nicola Pecchioni ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Recombinant Inbred Lines ◽  
Snp Array ◽  
Heading Date ◽  
Tiller Number ◽  
Genetic Maps ◽  
Yield Potential ◽  
Adaptation To Climate Change ◽  
Map Resolution ◽  
Mapping Populations

Traits such as plant height (PH), juvenile growth habit (GH), heading date (HD), and tiller number are important for both increasing yield potential and improving crop adaptation to climate change. In the present study, these traits were investigated by using the same bi-parental population at early (F2 and F2-derived F3 families) and late (F6 and F7, recombinant inbred lines, RILs) generations to detect quantitative trait loci (QTLs) and search for candidate genes. A total of 176 and 178 lines were genotyped by the wheat Illumina 25K Infinium SNP array. The two genetic maps spanned 2486.97 cM and 3732.84 cM in length, for the F2 and RILs, respectively. QTLs explaining the highest phenotypic variation were found on chromosomes 2B, 2D, 5A, and 7D for HD and GH, whereas those for PH were found on chromosomes 4B and 4D. Several QTL detected in the early generations (i.e., PH and tiller number) were not detected in the late generations as they were due to dominance effects. Some of the identified QTLs co-mapped to well-known adaptive genes (i.e., Ppd-1, Vrn-1, and Rht-1). Other putative candidate genes were identified for each trait, of which PINE1 and PIF4 may be considered new for GH and TTN in wheat. The use of a large F2 mapping population combined with NGS-based genotyping techniques could improve map resolution and allow closer QTL tagging.

scholarly journals An enhanced molecular marker based genetic map of perennial ryegrass (Lolium perenne) reveals comparative relationships with other Poaceae genomes

Genome ◽  
2002 ◽  
Vol 45 (2) ◽  
pp. 282-295 ◽  
Author(s):  
Elizabeth S Jones ◽  
Natalia L Mahoney ◽  
Michael D Hayward ◽  
Ian P Armstead ◽  
J Gilbert Jones ◽  
...  
Keyword(s):  
Molecular Marker ◽  
Linkage Map ◽  
Genetic Map ◽  
Lolium Perenne ◽  
Perennial Ryegrass ◽  
Genetic Linkage Map ◽  
Population Based ◽  
Genetic Maps ◽  
Linkage Groups ◽  
Integrated Genetic Map

A molecular-marker linkage map has been constructed for perennial ryegrass (Lolium perenne L.) using a one-way pseudo-testcross population based on the mating of a multiple heterozygous individual with a doubled haploid genotype. RFLP, AFLP, isoenzyme, and EST data from four collaborating laboratories within the International Lolium Genome Initiative were combined to produce an integrated genetic map containing 240 loci covering 811 cM on seven linkage groups. The map contained 124 codominant markers, of which 109 were heterologous anchor RFLP probes from wheat, barley, oat, and rice, allowing comparative relationships between perennial ryegrass and other Poaceae species to be inferred. The genetic maps of perennial ryegrass and the Triticeae cereals are highly conserved in terms of synteny and colinearity. This observation was supported by the general agreement of the syntenic relationships between perennial ryegrass, oat, and rice and those between the Triticeae and these species. A lower level of synteny and colinearity was observed between perennial ryegrass and oat compared with the Triticeae, despite the closer taxonomic affinity between these species. It is proposed that the linkage groups of perennial ryegrass be numbered in accordance with these syntenic relationships, to correspond to the homoeologous groups of the Triticeae cereals.Key words: Lolium perenne, genetic linkage map, RFLP, AFLP, conserved synteny.

scholarly journals A genetic linkage map for the domesticated silkworm, Bombyx mori, based on restriction fragment length polymorphisms

1995 ◽  
Vol 66 (2) ◽  
pp. 109-126 ◽  
Author(s):  
Jinrui Shi ◽  
David G. Heckel ◽  
Marian R. Goldsmith
Keyword(s):  
Linkage Map ◽  
Bombyx Mori ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Restriction Fragment Length Polymorphisms ◽  
Genetic Maps ◽  
Crossing Over ◽  
Linkage Groups ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

SummaryWe present data for the initial construction of a molecular linkage map for the domesticated silkworm, Bombyx mori, based on 52 progeny from an F2 cross from a pair mating of inbred strains p50 and C108, using restriction fragment length polymorphisms (RFLPs). The map contains 15 characterized single copy sequences, 36 anonymous sequences derived from a follicular cDNA library, and 10 loci corresponding to a low copy number retrotransposon, mag. The 15 linkage groups and 8 ungrouped loci account for 23 of the 28 chromosomes and span a total recombination length of 413 cM; 10 linkage groups were correlated with established classic genetic maps. Scoring data from Southern blots were analysed using two Pascal programs written specifically to analyse linkage data in Lepidoptera, where females are the heterogametic sex and have achiasmatic meiosis (no crossing-over). These first examine evidence for linkage by calculating the maximum lod score under the hypothesis that the two loci are linked over the likelihood under the hypothesis that the two loci assort independently, and then determine multilocus linkage maps for groups of putatively syntenic loci by calculating the maximum likelihood estimate of the recombination fractions and the log likelihood using the EM algorithm for a specified order of loci along the chromosome. In addition, the possibility of spurious linkage was exhaustively tested by searching for genotypes forbidden by the absence of crossing-over in one sex.

CHANGES IN MOISTURE, TEMPERATURE, AND QUALITY OF CORN SEED DURING HIGH-TEMPERATURE DRYING

1989 ◽  
Vol 69 (3) ◽  
pp. 749-761 ◽  
Author(s):  
ULRICH HERTER ◽  
JOSEPH S. BURRIS
Keyword(s):  
Seed Quality ◽  
Inbred Lines ◽  
Drying Time ◽  
Temperature Changes ◽  
Corn Seed ◽  
Seed Corn ◽  
Seed Moisture ◽  
The Relationship ◽  
High Temperature Drying

Mechanical drying has frequently caused injury in corn seed. Changes in seed moisture, temperature, and quality were determined for inbred lines A632, B73 and Mo17 to define the relationship between these variables. Ears harvested at ca. 48 and 38% seed moisture could be dried at 50 °C for 4–15 h and 18–24 h, respectively, before germination started to decline linearly with prolonged 50 °C drying. Drying time at 50 °C, seed moisture, or embryo moisture after 50 °C drying could be used equally well for prediction of seed quality. Seedling dry weights often declined even when seed was dried for only a few hours at 50 °C. Temperature measurements within seeds indicated that evaporation cooled the seed no more than 5 °C. Drying susceptibility of seed parents varied greatly between years.Key words: Moisture, temperature changes, seed corn, drying

Entomophilic nematodes, Diploscapter coronatus and Oscheius tipulae from Afghanistan

Zootaxa ◽  
2021 ◽  
Vol 4926 (3) ◽  
pp. 401-416
Author(s):  
MOHAMMAD HUSSAIN FALAHZADAH ◽  
EBRAHIM SHOKOOHI ◽  
GHOLAM HOSSEIN MORAVEJ ◽  
PHATU WILLIAM MASHELA ◽  
ABDUL KHALID MADADI ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Genetic Distance ◽  
Galleria Mellonella ◽  
Morphological Characters ◽  
Molecular Data ◽  
Soil Samples ◽  
The Body ◽  
Morphometric Data ◽  
Molecular Approaches ◽  
The Relationship

Several soil samples from different habitats in Badakhshan province of Afghanistan were collected to isolate and characterize bacteria feeding nematodes. The Galleria mellonella-baiting method was used for the isolation of the Afghan insect-associated nematodes. The nematodes were studied using morphological and morphometric data. The Oscheius specimen was characterized by a longer body (630–820 µm) and shorter pharynx (125–145 µm), whereas other morphological characters were not unusual. The Diploscapter specimen had an annulated cuticle, with lip region width 1.5 times shorter than the stoma, and had separated pharyngeal corpus from the isthmus and vulva located in the middle of the body. The molecular data were derived using three loci; 18S, 28S (D2/D3 segment), and ITS rRNA region, which were utilized to measure the genetic distance. The phylogenetic analysis was conducted to reconstruct the relationship tree. Both morphological and molecular approaches confirmed the identity of nematode isolates as Oscheius tipulae and Diploscapter coronatus. This is the first report of insect-associated nematodes from the soil of Afghanistan. Both species were capable of infecting and killing G. mellonella larvae in less than 96 h. 

scholarly journals High-resolution genetic maps ofLotus japonicusandL. burttiibased on re-sequencing of recombinant inbred lines

DNA Research ◽  
2016 ◽  
Vol 23 (5) ◽  
pp. 487-494 ◽  
Author(s):  
Niraj Shah ◽  
Hideki Hirakawa ◽  
Shohei Kusakabe ◽  
Niels Sandal ◽  
Jens Stougaard ◽  
...  
Keyword(s):  
High Resolution ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Genetic Maps

scholarly journals Quantitative Trait Loci for Resistance to Potato Dry Rot Caused by Fusarium sambucinum

Agronomy ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 203
Author(s):  
Sylwester Sobkowiak ◽  
Marta Janiszewska ◽  
Emil Stefańczyk ◽  
Iwona Wasilewicz-Flis ◽  
Jadwiga Śliwka
Keyword(s):  
Interval Mapping ◽  
Potato Cultivars ◽  
Genetic Maps ◽  
Dry Rot ◽  
Potato Genome ◽  
Polygenic Control ◽  
Efficient Breeding ◽  
Chromosome I ◽  
Mapping Populations ◽  
Important Disease

Tuber dry rot is an important disease of potato caused by soil and seed-borne pathogens of the Fusarium genus leading to losses that may reach 60% of the yield. The goal of this work was to study the inheritance of the dry rot resistance in two diploid potato hybrid populations (11-36 and 12-3) with complex pedigrees, including several wild Solanum spp. We used an aggressive isolate of F. sambucinum for phenotyping both progenies, parents, and standard potato cultivars in laboratory tuber tests, in three subsequent years. The QTL for dry rot resistance were mapped by interval mapping on existing genetic maps of both mapping populations. The most important and reproducible QTL for this trait was mapped on chromosome I and additional year- and population-specific QTL were mapped on chromosomes II, VII, IX, XI, and XII, confirming polygenic control of this resistance. This is the first study mapping the loci affecting tuber dry rot resistance in potato genome that can contribute to better understanding of potato-F. sambucinum interaction and to more efficient breeding of resistant potato cultivars.

Linkage mapping in diploid alfalfa (Medicago sativa)

Genome ◽  
1994 ◽  
Vol 37 (1) ◽  
pp. 61-71 ◽  
Author(s):  
C. S. Echt ◽  
K. K. Kidwell ◽  
S. J. Knapp ◽  
T. C. Osborn ◽  
T. J. McCoy
Keyword(s):  
Linkage Map ◽  
Linkage Mapping ◽  
Rapd Markers ◽  
Genome Mapping ◽  
Recurrent Parent ◽  
Backcross Population ◽  
Length Polymorphisms ◽  
Combined Use ◽  
A Genome ◽  
Genome Map

A genome map of cultivated alfalfa was constructed using segregating restriction fragment length polymorphisms (RFLPs) and random amplified polymorphic DNAs (RAPDs) in a diploid backcross population generated from noninbred parents. Among the 153 loci scored in 87 progeny, four segregation ratios were observed for codominant and dominant markers: 1:1, 1:2:1, 1:1:1:1, and 3:1. Deviations from expected Mendelian ratios (p < 0.05) were observed for 34% of the loci studied. A genome map was assembled from two separate linkage maps, each constructed from a subset of the segregation data. One linkage map was constructed from 46 RFLP and 40 RAPD markers segregating 1:1 from the F1 parent of the backcross and the other linkage map was constructed from 33 RFLP and 28 RAPD markers segregating 1:1 from the recurrent parent. Sixteen loci with alleles segregating 1:1 from both parents were used as locus bridges to align individual linkage groups between the two maps. The combined use of RFLPs and RAPDs was an effective method for developing an alfalfa genome map.Key words: genome mapping, RAPD, RFLP, locus bridges.

The relationship between genetic and cytogenetic maps of pea. I. Standard and translocation karyotypes

Genome ◽  
1997 ◽  
Vol 40 (5) ◽  
pp. 744-754 ◽  
Author(s):  
K. J. Hall ◽  
J. S. Parker ◽  
T. H. N. Ellis
Keyword(s):  
Linkage Map ◽  
Synaptonemal Complex ◽  
Chromosome Rearrangement ◽  
Translocation Line ◽  
Mitotic Chromosomes ◽  
Cytogenetic Map ◽  
Cytogenetical Study ◽  
Meiotic Analysis ◽  
Translocation Karyotypes ◽  
The Relationship

A detailed cytogenetical study of inbred lines of pea and their F1 hybrids has been undertaken to study the relationship between the cytogenetic map and the molecular linkage map. The mitotic karyotypes of a standard pea line, JI15, a translocation line, JI61, and line JI281, a line used in the production of a mapping population, are given. A chromosome rearrangement detected by cytogenetic analysis of mitotic chromosomes has been further defined by synaptonemal complex (SC) analysis and the study of metaphase I chromosome behaviour. This meiotic analysis has allowed a comparison of SC physical lengths, observed chiasma frequencies, and recombination frequencies, as estimated from the genetic map, as a means of comparing physical and genetic distances.Key words: Pisum, linkage map, cytogenetics, chromosome rearrangement, synaptonemal complex.

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